| PRSS21 |
| ID: | 1497288360 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 314 (264) | ||
| Sequences: | 4793 (3571.7) | ||
| Seq/Len: | 18.155 | ||
| Nf(neff/√len): | 219.8 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 18.155).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 171_C | 244_C | 4.750 | 1.00 |
| 52_R | 193_E | 3.872 | 1.00 |
| 69_V | 77_A | 3.645 | 1.00 |
| 67_C | 83_C | 3.632 | 1.00 |
| 122_S | 142_K | 3.549 | 1.00 |
| 71_L | 147_V | 3.325 | 1.00 |
| 76_W | 140_L | 3.141 | 1.00 |
| 78_L | 252_Q | 2.997 | 1.00 |
| 53_W | 193_E | 2.969 | 1.00 |
| 100_F | 147_V | 2.959 | 1.00 |
| 47_D | 192_Q | 2.896 | 1.00 |
| 58_S | 65_H | 2.783 | 1.00 |
| 136_Y | 277_H | 2.775 | 1.00 |
| 56_Q | 174_T | 2.663 | 1.00 |
| 128_P | 284_L | 2.540 | 1.00 |
| 120_F | 144_S | 2.429 | 1.00 |
| 258_W | 270_V | 2.395 | 1.00 |
| 194_V | 231_K | 2.323 | 1.00 |
| 70_S | 252_Q | 2.310 | 1.00 |
| 75_R | 142_K | 2.305 | 1.00 |
| 57_G | 100_F | 2.296 | 1.00 |
| 79_T | 84_F | 2.262 | 1.00 |
| 123_N | 142_K | 2.253 | 1.00 |
| 57_G | 69_V | 2.231 | 1.00 |
| 84_F | 139_A | 2.205 | 1.00 |
| 121_V | 141_V | 2.200 | 1.00 |
| 76_W | 142_K | 2.177 | 1.00 |
| 99_Q | 118_R | 2.109 | 1.00 |
| 70_S | 156_I | 2.062 | 1.00 |
| 143_L | 147_V | 2.039 | 1.00 |
| 156_I | 243_A | 2.004 | 1.00 |
| 135_P | 220_D | 1.950 | 1.00 |
| 173_V | 194_V | 1.946 | 1.00 |
| 156_I | 252_Q | 1.919 | 1.00 |
| 122_S | 144_S | 1.889 | 1.00 |
| 232_D | 269_G | 1.868 | 1.00 |
| 220_D | 276_H | 1.858 | 1.00 |
| 174_T | 243_A | 1.851 | 1.00 |
| 173_V | 233_A | 1.849 | 1.00 |
| 42_I | 45_G | 1.843 | 1.00 |
| 75_R | 146_P | 1.828 | 1.00 |
| 82_H | 238_S | 1.826 | 1.00 |
| 181_E | 264_R | 1.781 | 1.00 |
| 71_L | 100_F | 1.771 | 1.00 |
| 173_V | 256_V | 1.771 | 1.00 |
| 60_R | 65_H | 1.758 | 1.00 |
| 119_Y | 147_V | 1.710 | 1.00 |
| 197_A | 225_G | 1.677 | 1.00 |
| 73_S | 76_W | 1.661 | 1.00 |
| 234_C | 262_C | 1.659 | 1.00 |
| 125_Y | 284_L | 1.656 | 1.00 |
| 278_F | 282_Q | 1.642 | 1.00 |
| 33_C | 157_C | 1.541 | 1.00 |
| 138_I | 272_T | 1.531 | 1.00 |
| 171_C | 198_I | 1.495 | 1.00 |
| 221_M | 258_W | 1.492 | 1.00 |
| 173_V | 196_V | 1.489 | 1.00 |
| 239_G | 242_L | 1.479 | 1.00 |
| 99_Q | 102_Q | 1.473 | 1.00 |
| 119_Y | 144_S | 1.470 | 1.00 |
| 51_G | 103_L | 1.461 | 1.00 |
| 273_N | 276_H | 1.455 | 1.00 |
| 70_S | 255_V | 1.453 | 1.00 |
| 201_N | 273_N | 1.436 | 1.00 |
| 53_W | 250_W | 1.436 | 1.00 |
| 149_Y | 155_P | 1.433 | 1.00 |
| 74_H | 146_P | 1.427 | 1.00 |
| 199_I | 268_P | 1.423 | 1.00 |
| 135_P | 218_F | 1.414 | 1.00 |
| 136_Y | 220_D | 1.400 | 0.99 |
| 106_M | 189_H | 1.395 | 0.99 |
| 82_H | 137_D | 1.387 | 0.99 |
| 56_Q | 191_L | 1.379 | 0.99 |
| 100_F | 153_I | 1.374 | 0.99 |
| 200_N | 203_M | 1.352 | 0.99 |
| 179_I | 192_Q | 1.345 | 0.99 |
| 204_C | 223_C | 1.343 | 0.99 |
| 196_V | 271_Y | 1.343 | 0.99 |
| 71_L | 77_A | 1.342 | 0.99 |
| 188_P | 192_Q | 1.332 | 0.99 |
| 138_I | 277_H | 1.328 | 0.99 |
| 51_G | 152_H | 1.319 | 0.99 |
| 47_D | 190_T | 1.315 | 0.99 |
| 77_A | 98_V | 1.310 | 0.99 |
| 272_T | 277_H | 1.308 | 0.99 |
| 127_S | 136_Y | 1.298 | 0.99 |
| 170_D | 195_Q | 1.291 | 0.99 |
| 125_Y | 142_K | 1.287 | 0.99 |
| 34_G | 55_W | 1.283 | 0.99 |
| 220_D | 277_H | 1.265 | 0.99 |
| 47_D | 103_L | 1.253 | 0.99 |
| 48_A | 53_W | 1.250 | 0.99 |
| 56_Q | 68_G | 1.239 | 0.98 |
| 172_W | 195_Q | 1.238 | 0.98 |
| 158_L | 278_F | 1.231 | 0.98 |
| 53_W | 174_T | 1.226 | 0.98 |
| 80_A | 255_V | 1.213 | 0.98 |
| 197_A | 226_N | 1.212 | 0.98 |
| 171_C | 253_I | 1.207 | 0.98 |
| 104_T | 189_H | 1.202 | 0.98 |
| 226_N | 229_G | 1.194 | 0.98 |
| 79_T | 139_A | 1.192 | 0.98 |
| 255_V | 272_T | 1.187 | 0.98 |
| 57_G | 77_A | 1.183 | 0.98 |
| 222_V | 242_L | 1.179 | 0.98 |
| 43_V | 263_G | 1.178 | 0.98 |
| 233_A | 256_V | 1.159 | 0.97 |
| 256_V | 259_G | 1.150 | 0.97 |
| 98_V | 121_V | 1.148 | 0.97 |
| 84_F | 124_I | 1.147 | 0.97 |
| 57_G | 98_V | 1.141 | 0.97 |
| 60_R | 105_S | 1.134 | 0.97 |
| 234_C | 237_D | 1.125 | 0.97 |
| 137_D | 238_S | 1.123 | 0.97 |
| 46_E | 193_E | 1.118 | 0.96 |
| 55_W | 243_A | 1.114 | 0.96 |
| 59_L | 98_V | 1.102 | 0.96 |
| 219_G | 276_H | 1.102 | 0.96 |
| 84_F | 141_V | 1.101 | 0.96 |
| 256_V | 271_Y | 1.096 | 0.96 |
| 126_L | 139_A | 1.090 | 0.96 |
| 71_L | 149_Y | 1.083 | 0.96 |
| 261_G | 264_R | 1.078 | 0.95 |
| 117_T | 153_I | 1.068 | 0.95 |
| 119_Y | 143_L | 1.068 | 0.95 |
| 125_Y | 140_L | 1.056 | 0.95 |
| 104_T | 190_T | 1.052 | 0.95 |
| 80_A | 83_C | 1.050 | 0.94 |
| 274_I | 281_I | 1.040 | 0.94 |
| 129_R | 136_Y | 1.032 | 0.94 |
| 172_W | 250_W | 1.026 | 0.94 |
| 237_D | 240_G | 1.014 | 0.93 |
| 55_W | 157_C | 1.009 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4nzqA | 1 | 0.8662 | 100 | 0.317 | Contact Map |
| 3nxpA | 1 | 0.8631 | 100 | 0.323 | Contact Map |
| 4kkdA | 2 | 0.879 | 100 | 0.325 | Contact Map |
| 2xxlA | 2 | 0.8822 | 100 | 0.327 | Contact Map |
| 2f83A | 1 | 0.7803 | 100 | 0.327 | Contact Map |
| 2b9lA | 1 | 0.9172 | 100 | 0.333 | Contact Map |
| 2vntA | 6 | 0.7834 | 100 | 0.335 | Contact Map |
| 1z8gA | 1 | 0.8981 | 100 | 0.336 | Contact Map |
| 2bdyA | 1 | 0.828 | 100 | 0.338 | Contact Map |
| 4durA | 2 | 0.8471 | 100 | 0.339 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)