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OPENSEQ.org

mglu

ID: 1496427509 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 822 (787)
Sequences: 1331 (900.6)
Seq/Len: 1.691
Nf(neff/√len): 32.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.691).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
145_G407_M3.4111.00
175_T192_V2.9471.00
530_C537_C2.4111.00
710_A714_M2.3711.00
531_C537_C2.2891.00
382_N385_L2.2610.99
64_Y67_Q2.2390.99
445_N453_T2.1550.99
515_C537_C2.1390.99
346_P359_W2.1390.99
231_F235_S2.1230.99
549_C552_C2.1080.99
405_Y409_Y2.0980.99
155_A174_A2.0840.99
171_A188_F2.0730.99
530_C534_C2.0470.99
359_W363_F2.0450.99
71_A337_F2.0300.99
79_I86_L2.0040.99
182_K189_M1.9920.98
180_S461_D1.9820.98
818_F821_K1.9520.98
175_T195_D1.9520.98
446_F454_I1.9400.98
148_G152_S1.9330.98
86_L91_L1.9050.98
511_C515_C1.8940.98
534_C537_C1.8900.98
181_D184_L1.8780.97
223_Y227_G1.8590.97
515_C530_C1.8280.97
409_Y412_H1.8170.97
524_R527_E1.8140.97
400_V404_I1.8140.97
272_R299_F1.8110.97
456_F460_G1.7920.96
294_G297_G1.7830.96
755_T775_A1.7730.96
404_I408_A1.7380.95
195_D466_Y1.7200.95
785_W788_F1.6850.94
531_C534_C1.6730.94
198_Q223_Y1.6660.94
485_G500_W1.6640.94
33_G38_G1.6610.94
79_I405_Y1.6460.93
676_S679_K1.6290.93
799_I802_M1.6280.93
457_D460_G1.6250.93
675_G678_K1.6170.93
629_Y811_T1.6160.93
500_W507_I1.6160.93
711_L746_Y1.6130.93
289_A293_L1.6070.92
709_V713_I1.5960.92
289_A292_R1.5940.92
216_A231_F1.5890.92
509_S515_C1.5860.92
87_P412_H1.5680.91
182_K442_M1.5620.91
38_G41_F1.5610.91
524_R533_T1.5600.91
149_P174_A1.5520.91
80_N91_L1.5490.90
549_C565_C1.5420.90
79_I87_P1.5410.90
39_A72_M1.5380.90
736_T802_M1.5220.89
515_C534_C1.5200.89
466_Y485_G1.5200.89
775_A779_Y1.5200.89
92_G97_D1.5140.89
147_I407_M1.5000.88
607_T610_V1.4970.88
359_W365_C1.4950.88
706_G710_A1.4910.88
515_C531_C1.4850.88
552_C565_C1.4850.88
339_D342_L1.4850.88
406_S456_F1.4730.87
765_P770_E1.4670.87
686_R689_S1.4610.87
207_K238_E1.4600.87
403_A408_A1.4600.87
146_V162_L1.4550.86
77_E81_S1.4520.86
704_Q711_L1.4520.86
78_R401_I1.4420.86
623_A656_A1.4340.85
806_V809_S1.4300.85
511_C530_C1.4250.85
511_C537_C1.4220.85
242_I587_A1.4110.84
77_E82_D1.4100.84
596_F600_V1.4090.84
171_A190_R1.4080.84
34_D37_I1.4040.84
758_A762_R1.4000.83
402_N448_G1.3930.83
34_D38_G1.3910.83
75_T86_L1.3870.82
508_R513_E1.3870.82
704_Q746_Y1.3860.82
216_A228_M1.3840.82
243_A271_A1.3810.82
261_L290_M1.3800.82
203_V206_V1.3750.82
405_Y408_A1.3710.81
365_C381_C1.3700.81
40_L98_S1.3700.81
611_K673_L1.3580.81
180_S190_R1.3560.80
590_G629_Y1.3520.80
581_I585_V1.3460.80
511_C531_C1.3450.80
179_L195_D1.3400.79
408_A411_L1.3370.79
92_G99_C1.3370.79
224_G466_Y1.3280.79
80_N87_P1.3270.78
35_I38_G1.3250.78
150_G173_S1.3220.78
188_F195_D1.3190.78
34_D39_A1.3180.78
515_C518_G1.3150.78
468_I485_G1.3150.78
37_I40_L1.3120.77
679_K802_M1.3090.77
403_A410_G1.3090.77
456_F461_D1.3080.77
492_L495_D1.3070.77
788_F792_Y1.3070.77
174_A190_R1.2970.76
80_N86_L1.2930.76
688_M692_A1.2930.76
180_S188_F1.2930.76
507_I511_C1.2930.76
594_T597_V1.2880.76
78_R82_D1.2840.75
227_G277_F1.2840.75
666_T669_I1.2830.75
64_Y68_R1.2820.75
74_H78_R1.2820.75
767_N770_E1.2810.75
751_I781_T1.2780.75
507_I515_C1.2760.75
152_S168_P1.2720.74
148_G159_Q1.2720.74
147_I170_I1.2710.74
666_T768_F1.2610.73
360_Q366_R1.2600.73
625_I637_A1.2600.73
679_K737_N1.2530.73
71_A75_T1.2460.72
512_S537_C1.2400.72
457_D461_D1.2380.71
509_S537_C1.2350.71
679_K736_T1.2300.71
337_F340_Y1.2290.71
446_F456_F1.2270.70
758_A769_N1.2270.70
530_C544_F1.2260.70
415_Q418_L1.2210.70
518_G534_C1.2190.70
673_L765_P1.2170.69
517_K543_V1.2170.69
36_I39_A1.2160.69
155_A159_Q1.2110.69
762_R769_N1.2070.69
693_Q754_C1.2040.68
287_L290_M1.2010.68
406_S446_F1.2010.68
440_S443_K1.2010.68
175_T227_G1.2000.68
546_E800_I1.1990.68
179_L190_R1.1980.68
195_D223_Y1.1980.68
148_G168_P1.1970.68
785_W809_S1.1950.67
185_F190_R1.1950.67
264_L771_A1.1950.67
708_I745_G1.1920.67
658_S661_A1.1920.67
509_S530_C1.1920.67
157_Q162_L1.1910.67
467_E483_N1.1910.67
647_Q651_I1.1900.67
403_A406_S1.1890.67
220_E224_G1.1880.67
72_M400_V1.1870.67
541_E551_A1.1860.67
79_I408_A1.1820.66
189_M438_L1.1820.66
73_L93_C1.1820.66
149_P155_A1.1810.66
678_K802_M1.1810.66
785_W792_Y1.1760.66
516_E519_Q1.1730.65
511_C534_C1.1700.65
612_S790_P1.1690.65
615_R668_R1.1650.65
755_T779_Y1.1640.64
556_S567_L1.1640.64
174_A273_V1.1640.64
470_N498_E1.1560.64
614_S668_R1.1560.64
79_I91_L1.1560.64
635_L779_Y1.1530.63
159_Q163_Q1.1530.63
675_G802_M1.1520.63
578_P582_A1.1520.63
37_I41_F1.1520.63
193_P203_V1.1500.63
530_C540_N1.1490.63
426_C434_G1.1470.63
86_L89_I1.1460.63
762_R773_Y1.1450.63
290_M295_L1.1440.63
235_S275_A1.1430.62
522_V535_T1.1430.62
214_V703_I1.1420.62
330_Q467_E1.1420.62
198_Q722_D1.1410.62
414_M440_S1.1400.62
360_Q367_L1.1380.62
666_T774_I1.1380.62
209_Y507_I1.1370.62
531_C544_F1.1370.62
182_K457_D1.1360.62
685_P688_M1.1350.62
676_S800_I1.1350.62
80_N88_N1.1350.62
264_L274_V1.1340.62
302_L334_V1.1330.61
175_T188_F1.1330.61
201_A327_I1.1330.61
65_G68_R1.1330.61
214_V231_F1.1300.61
444_T463_P1.1280.61
206_V302_L1.1280.61
175_T223_Y1.1270.61
382_N387_L1.1270.61
412_H416_M1.1250.61
334_V337_F1.1250.61
175_T179_L1.1250.61
57_C99_C1.1240.60
257_F289_A1.1230.60
482_I485_G1.1230.60
803_C812_V1.1230.60
758_A768_F1.1220.60
33_G37_I1.1190.60
512_S515_C1.1190.60
360_Q365_C1.1190.60
540_N543_V1.1180.60
666_T673_L1.1170.60
218_H228_M1.1160.60
660_S663_V1.1110.59
632_T807_S1.1110.59
537_C544_F1.1100.59
41_F72_M1.1080.59
34_D40_L1.1080.59
344_L353_P1.1070.59
66_I70_E1.1040.58
188_F227_G1.1040.58
77_E593_A1.1020.58
76_L460_G1.1010.58
179_L307_W1.1010.58
675_G679_K1.1000.58
129_C132_G1.1000.58
536_P542_Y1.0990.58
44_H57_C1.0990.58
176_S223_Y1.0990.58
614_S669_I1.0980.58
766_A775_A1.0980.58
269_P274_V1.0950.57
614_S621_I1.0950.57
42_S68_R1.0940.57
86_L412_H1.0930.57
147_I152_S1.0920.57
437_L442_M1.0920.57
405_Y412_H1.0910.57
469_M492_L1.0910.57
360_Q363_F1.0910.57
264_L284_R1.0900.57
597_V608_P1.0890.57
97_D105_A1.0880.57
74_H401_I1.0870.57
704_Q785_W1.0850.56
326_T341_Y1.0840.56
309_D328_K1.0790.56
92_G95_I1.0770.56
75_T79_I1.0760.55
676_S682_T1.0750.55
395_S398_G1.0750.55
195_D227_G1.0750.55
769_N776_F1.0710.55
231_F277_F1.0700.55
587_A590_G1.0690.55
765_P768_F1.0690.55
33_G36_I1.0670.55
205_I772_K1.0670.55
77_E80_N1.0660.54
705_L709_V1.0660.54
676_S736_T1.0660.54
610_V622_L1.0660.54
518_G530_C1.0650.54
677_K799_I1.0650.54
203_V240_I1.0640.54
631_C636_I1.0630.54
619_Y648_R1.0630.54
635_L647_Q1.0620.54
81_S487_W1.0610.54
651_I764_V1.0610.54
209_Y273_V1.0610.54
122_E131_D1.0600.54
780_T808_L1.0600.54
202_M206_V1.0560.53
582_A587_A1.0550.53
570_V638_K1.0540.53
600_V604_Y1.0540.53
655_P773_Y1.0530.53
206_V210_N1.0520.53
605_R619_Y1.0520.53
235_S240_I1.0520.53
417_S426_C1.0510.53
95_I99_C1.0510.53
164_L169_Q1.0500.53
83_P88_N1.0500.53
75_T80_N1.0500.53
792_Y795_S1.0500.53
717_P785_W1.0490.53
214_V302_L1.0480.53
400_V408_A1.0480.53
86_L408_A1.0470.52
703_I707_I1.0470.52
612_S652_G1.0460.52
621_I652_G1.0430.52
544_F550_K1.0430.52
559_T563_T1.0430.52
618_C768_F1.0410.52
718_D734_N1.0410.52
573_L580_P1.0410.52
188_F191_V1.0400.52
76_L93_C1.0400.52
37_I42_S1.0390.52
188_F192_V1.0360.51
232_K245_S1.0360.51
36_I43_V1.0350.51
771_A775_A1.0330.51
689_S692_A1.0320.51
195_D198_Q1.0320.51
48_T56_K1.0320.51
751_I761_T1.0300.51
531_C542_Y1.0300.51
748_G777_T1.0290.51
209_Y620_I1.0290.51
400_V403_A1.0280.50
246_Y263_K1.0270.50
152_S158_V1.0250.50
758_A778_M1.0250.50
761_T775_A1.0250.50
614_S666_T1.0230.50
379_K382_N1.0230.50
519_Q536_P1.0230.50
234_M237_K1.0220.50
185_F726_I1.0210.50
33_G39_A1.0210.50
106_L443_K1.0200.50
598_T602_I1.0180.49
341_Y344_L1.0180.49
191_V403_A1.0160.49
195_D199_A1.0160.49
518_G537_C1.0150.49
470_N484_V1.0150.49
242_I268_L1.0140.49
567_L686_R1.0140.49
526_G533_T1.0120.49
624_G775_A1.0100.48
545_D678_K1.0100.48
717_P776_F1.0100.48
74_H86_L1.0090.48
537_C540_N1.0080.48
201_A216_A1.0080.48
223_Y305_D1.0070.48
629_Y701_I1.0060.48
265_T293_L1.0060.48
193_P198_Q1.0060.48
70_E73_L1.0050.48
53_H56_K1.0050.48
609_V766_A1.0050.48
271_A484_V1.0050.48
155_A162_L1.0020.48
146_V169_Q1.0020.48
203_V300_L1.0010.48
224_G227_G1.0000.47
171_A192_V1.0000.47
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2e4uA20.61071000.511Contact Map
3ks9A20.54261000.661Contact Map
4xaqA20.53041000.664Contact Map
3sm9A10.52311000.666Contact Map
5c5cA101000.67Contact Map
4or2A20.42821000.694Contact Map
1dp4A20.48781000.696Contact Map
4oo9A10.28831000.712Contact Map
1jdpA20.44891000.722Contact Map
4ms4B10.47811000.739Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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