| TM_SLC26A4 resubmit |
| ID: | 1496226080 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 420 (395) | ||
| Sequences: | 21380 (11685.3) | ||
| Seq/Len: | 54.127 | ||
| Nf(neff/√len): | 588.0 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 54.127).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 152_V | 188_G | 3.271 | 1.00 |
| 107_L | 309_G | 2.739 | 1.00 |
| 117_G | 299_I | 2.618 | 1.00 |
| 41_F | 338_I | 2.501 | 1.00 |
| 401_V | 413_A | 2.443 | 1.00 |
| 36_P | 304_S | 2.200 | 1.00 |
| 38_L | 338_I | 2.109 | 1.00 |
| 122_G | 295_N | 2.075 | 1.00 |
| 331_G | 336_A | 2.067 | 1.00 |
| 186_T | 190_L | 2.000 | 1.00 |
| 38_L | 341_A | 1.959 | 1.00 |
| 4_G | 339_I | 1.958 | 1.00 |
| 195_C | 219_V | 1.951 | 1.00 |
| 107_L | 306_I | 1.948 | 1.00 |
| 190_L | 194_V | 1.936 | 1.00 |
| 192_I | 369_A | 1.927 | 1.00 |
| 176_N | 179_D | 1.893 | 1.00 |
| 392_A | 396_V | 1.877 | 1.00 |
| 39_T | 307_F | 1.876 | 1.00 |
| 110_G | 306_I | 1.862 | 1.00 |
| 342_A | 346_I | 1.846 | 1.00 |
| 32_S | 316_A | 1.814 | 1.00 |
| 180_T | 185_F | 1.797 | 1.00 |
| 114_L | 299_I | 1.772 | 1.00 |
| 225_A | 229_G | 1.758 | 1.00 |
| 397_F | 417_F | 1.752 | 1.00 |
| 223_A | 362_V | 1.735 | 1.00 |
| 47_R | 51_V | 1.728 | 1.00 |
| 255_P | 258_L | 1.712 | 1.00 |
| 108_L | 266_S | 1.709 | 1.00 |
| 308_S | 313_C | 1.658 | 1.00 |
| 34_F | 341_A | 1.646 | 1.00 |
| 54_F | 57_V | 1.641 | 1.00 |
| 13_L | 17_A | 1.634 | 1.00 |
| 326_Q | 336_A | 1.633 | 1.00 |
| 112_I | 267_F | 1.623 | 1.00 |
| 34_F | 345_M | 1.617 | 1.00 |
| 115_I | 119_L | 1.617 | 1.00 |
| 241_V | 245_P | 1.596 | 1.00 |
| 43_F | 303_I | 1.595 | 1.00 |
| 236_Y | 240_I | 1.591 | 1.00 |
| 251_P | 254_P | 1.589 | 1.00 |
| 13_L | 18_Y | 1.589 | 1.00 |
| 239_G | 242_K | 1.583 | 1.00 |
| 158_K | 176_N | 1.571 | 1.00 |
| 250_P | 254_P | 1.559 | 1.00 |
| 226_I | 230_A | 1.556 | 1.00 |
| 114_L | 302_G | 1.553 | 1.00 |
| 339_I | 343_I | 1.545 | 1.00 |
| 111_I | 115_I | 1.525 | 1.00 |
| 181_N | 184_D | 1.518 | 1.00 |
| 401_V | 405_L | 1.510 | 1.00 |
| 379_C | 382_P | 1.486 | 1.00 |
| 171_V | 175_Q | 1.483 | 1.00 |
| 393_V | 396_V | 1.466 | 1.00 |
| 114_L | 306_I | 1.461 | 1.00 |
| 165_S | 168_Y | 1.458 | 1.00 |
| 11_A | 14_Q | 1.446 | 1.00 |
| 284_Y | 323_T | 1.437 | 1.00 |
| 382_P | 386_R | 1.437 | 1.00 |
| 184_D | 361_S | 1.435 | 1.00 |
| 194_V | 198_V | 1.426 | 1.00 |
| 236_Y | 239_G | 1.424 | 1.00 |
| 111_I | 306_I | 1.422 | 1.00 |
| 10_V | 13_L | 1.419 | 1.00 |
| 183_A | 186_T | 1.416 | 1.00 |
| 182_L | 186_T | 1.415 | 1.00 |
| 118_G | 299_I | 1.414 | 1.00 |
| 3_S | 331_G | 1.381 | 0.99 |
| 396_V | 399_C | 1.379 | 0.99 |
| 34_F | 38_L | 1.376 | 0.99 |
| 190_L | 226_I | 1.369 | 0.99 |
| 307_F | 311_F | 1.368 | 0.99 |
| 406_G | 409_L | 1.362 | 0.99 |
| 39_T | 43_F | 1.361 | 0.99 |
| 195_C | 199_K | 1.359 | 0.99 |
| 221_I | 225_A | 1.357 | 0.99 |
| 191_T | 365_A | 1.356 | 0.99 |
| 37_I | 341_A | 1.350 | 0.99 |
| 200_E | 204_R | 1.349 | 0.99 |
| 18_Y | 21_L | 1.343 | 0.99 |
| 31_Y | 348_I | 1.339 | 0.99 |
| 48_H | 319_A | 1.336 | 0.99 |
| 14_Q | 30_L | 1.329 | 0.99 |
| 184_D | 188_G | 1.327 | 0.99 |
| 53_P | 57_V | 1.323 | 0.99 |
| 385_W | 389_K | 1.322 | 0.99 |
| 249_L | 252_E | 1.318 | 0.99 |
| 284_Y | 327_E | 1.316 | 0.99 |
| 235_N | 238_A | 1.312 | 0.99 |
| 223_A | 227_S | 1.312 | 0.99 |
| 393_V | 397_F | 1.310 | 0.99 |
| 231_N | 234_K | 1.304 | 0.99 |
| 194_V | 222_I | 1.304 | 0.99 |
| 23_A | 62_G | 1.303 | 0.99 |
| 398_T | 414_G | 1.297 | 0.99 |
| 117_G | 302_G | 1.293 | 0.99 |
| 405_L | 409_L | 1.289 | 0.99 |
| 94_R | 97_A | 1.288 | 0.99 |
| 199_K | 373_G | 1.286 | 0.99 |
| 112_I | 270_A | 1.282 | 0.99 |
| 46_S | 51_V | 1.279 | 0.99 |
| 41_F | 337_G | 1.279 | 0.99 |
| 250_P | 253_L | 1.277 | 0.99 |
| 188_G | 365_A | 1.277 | 0.99 |
| 385_W | 391_D | 1.276 | 0.99 |
| 12_T | 18_Y | 1.274 | 0.99 |
| 400_I | 404_I | 1.273 | 0.99 |
| 189_L | 193_V | 1.269 | 0.99 |
| 242_K | 245_P | 1.267 | 0.99 |
| 395_W | 398_T | 1.266 | 0.99 |
| 13_L | 19_A | 1.266 | 0.99 |
| 14_Q | 19_A | 1.250 | 0.99 |
| 237_N | 240_I | 1.240 | 0.98 |
| 218_I | 222_I | 1.239 | 0.98 |
| 146_V | 170_L | 1.230 | 0.98 |
| 243_S | 246_R | 1.228 | 0.98 |
| 192_I | 365_A | 1.225 | 0.98 |
| 32_S | 311_F | 1.219 | 0.98 |
| 225_A | 228_Y | 1.216 | 0.98 |
| 193_V | 197_A | 1.215 | 0.98 |
| 394_I | 399_C | 1.209 | 0.98 |
| 249_L | 254_P | 1.207 | 0.98 |
| 398_T | 401_V | 1.206 | 0.98 |
| 3_S | 336_A | 1.205 | 0.98 |
| 19_A | 23_A | 1.204 | 0.98 |
| 10_V | 14_Q | 1.201 | 0.98 |
| 44_G | 49_I | 1.199 | 0.98 |
| 49_I | 340_S | 1.199 | 0.98 |
| 396_V | 400_I | 1.198 | 0.98 |
| 191_T | 219_V | 1.197 | 0.98 |
| 141_A | 371_L | 1.197 | 0.98 |
| 156_S | 159_N | 1.196 | 0.98 |
| 53_P | 56_V | 1.196 | 0.98 |
| 303_I | 307_F | 1.196 | 0.98 |
| 281_G | 297_E | 1.196 | 0.98 |
| 116_F | 270_A | 1.195 | 0.98 |
| 14_Q | 18_Y | 1.191 | 0.98 |
| 346_I | 350_A | 1.190 | 0.98 |
| 13_L | 21_L | 1.187 | 0.98 |
| 389_K | 393_V | 1.181 | 0.98 |
| 14_Q | 17_A | 1.180 | 0.98 |
| 172_E | 175_Q | 1.178 | 0.98 |
| 229_G | 232_L | 1.173 | 0.98 |
| 290_Y | 293_D | 1.169 | 0.97 |
| 157_T | 160_Y | 1.161 | 0.97 |
| 192_I | 196_M | 1.159 | 0.97 |
| 166_I | 169_T | 1.154 | 0.97 |
| 62_G | 66_L | 1.151 | 0.97 |
| 19_A | 22_A | 1.151 | 0.97 |
| 171_V | 174_F | 1.146 | 0.97 |
| 168_Y | 172_E | 1.145 | 0.97 |
| 47_R | 50_S | 1.144 | 0.97 |
| 42_I | 338_I | 1.144 | 0.97 |
| 112_I | 266_S | 1.142 | 0.97 |
| 109_V | 113_Q | 1.135 | 0.97 |
| 122_G | 126_R | 1.133 | 0.97 |
| 52_G | 57_V | 1.126 | 0.97 |
| 138_T | 399_C | 1.126 | 0.97 |
| 338_I | 342_A | 1.125 | 0.97 |
| 395_W | 399_C | 1.124 | 0.97 |
| 187_A | 223_A | 1.123 | 0.97 |
| 112_I | 263_L | 1.119 | 0.96 |
| 5_V | 343_I | 1.119 | 0.96 |
| 219_V | 223_A | 1.116 | 0.96 |
| 243_S | 247_G | 1.116 | 0.96 |
| 63_S | 67_S | 1.112 | 0.96 |
| 246_R | 249_L | 1.109 | 0.96 |
| 204_R | 207_H | 1.103 | 0.96 |
| 168_Y | 171_V | 1.102 | 0.96 |
| 198_V | 218_I | 1.100 | 0.96 |
| 388_N | 391_D | 1.097 | 0.96 |
| 185_F | 189_L | 1.094 | 0.96 |
| 97_A | 100_L | 1.093 | 0.96 |
| 64_V | 265_A | 1.092 | 0.96 |
| 224_T | 227_S | 1.090 | 0.96 |
| 120_Q | 123_F | 1.085 | 0.96 |
| 191_T | 362_V | 1.085 | 0.96 |
| 335_V | 338_I | 1.083 | 0.96 |
| 397_F | 401_V | 1.080 | 0.95 |
| 10_V | 17_A | 1.080 | 0.95 |
| 8_G | 343_I | 1.080 | 0.95 |
| 106_T | 309_G | 1.079 | 0.95 |
| 105_L | 109_V | 1.079 | 0.95 |
| 195_C | 369_A | 1.078 | 0.95 |
| 131_P | 135_G | 1.078 | 0.95 |
| 115_I | 118_G | 1.077 | 0.95 |
| 226_I | 229_G | 1.076 | 0.95 |
| 306_I | 310_F | 1.075 | 0.95 |
| 66_L | 69_A | 1.073 | 0.95 |
| 224_T | 357_P | 1.071 | 0.95 |
| 145_L | 368_I | 1.070 | 0.95 |
| 100_L | 103_S | 1.067 | 0.95 |
| 170_L | 174_F | 1.065 | 0.95 |
| 198_V | 215_I | 1.063 | 0.95 |
| 14_Q | 21_L | 1.060 | 0.95 |
| 393_V | 398_T | 1.060 | 0.95 |
| 288_Y | 327_E | 1.055 | 0.95 |
| 392_A | 395_W | 1.055 | 0.95 |
| 396_V | 402_S | 1.054 | 0.95 |
| 134_G | 383_R | 1.053 | 0.95 |
| 110_G | 117_G | 1.051 | 0.94 |
| 182_L | 185_F | 1.049 | 0.94 |
| 131_P | 391_D | 1.048 | 0.94 |
| 52_G | 55_P | 1.045 | 0.94 |
| 186_T | 189_L | 1.045 | 0.94 |
| 60_M | 64_V | 1.045 | 0.94 |
| 52_G | 322_R | 1.042 | 0.94 |
| 399_C | 402_S | 1.040 | 0.94 |
| 335_V | 339_I | 1.037 | 0.94 |
| 167_I | 171_V | 1.035 | 0.94 |
| 206_R | 209_I | 1.035 | 0.94 |
| 108_L | 262_M | 1.030 | 0.94 |
| 194_V | 219_V | 1.029 | 0.94 |
| 252_E | 255_P | 1.029 | 0.94 |
| 11_A | 19_A | 1.025 | 0.93 |
| 120_Q | 295_N | 1.025 | 0.93 |
| 8_G | 13_L | 1.024 | 0.93 |
| 103_S | 309_G | 1.023 | 0.93 |
| 172_E | 176_N | 1.022 | 0.93 |
| 174_F | 177_I | 1.022 | 0.93 |
| 151_I | 360_K | 1.021 | 0.93 |
| 179_D | 182_L | 1.021 | 0.93 |
| 58_S | 314_F | 1.021 | 0.93 |
| 249_L | 253_L | 1.015 | 0.93 |
| 227_S | 232_L | 1.009 | 0.93 |
| 24_V | 314_F | 1.008 | 0.93 |
| 17_A | 20_L | 1.007 | 0.93 |
| 102_A | 106_T | 1.007 | 0.93 |
| 228_Y | 357_P | 1.007 | 0.93 |
| 151_I | 364_A | 1.004 | 0.93 |
| 188_G | 191_T | 1.002 | 0.92 |
| 109_V | 270_A | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- See coupling matrix (for protein design or mutagenesis)
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3qe7A | 1 | 0.8571 | 100 | 0.434 | Contact Map |
| 4us3A | 1 | 0.8071 | 20.3 | 0.958 | Contact Map |
| 1btsA | 1 | 0.05 | 13.6 | 0.961 | Contact Map |
| 4k0jA | 6 | 0.3429 | 7 | 0.966 | Contact Map |
| 4dx5A | 3 | 0.3905 | 5.8 | 0.967 | Contact Map |
| 3ne5A | 1 | 0.3762 | 3.2 | 0.971 | Contact Map |
| 1a87A | 1 | 0.1429 | 3.2 | 0.971 | Contact Map |
| 3w9iA | 6 | 0.3667 | 3 | 0.972 | Contact Map |
| 1colA | 2 | 0.1357 | 2.8 | 0.972 | Contact Map |
| 1bzkA | 1 | 0.0595 | 2.6 | 0.972 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)