| mATGL300 |
| ID: | 1494590616 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 300 (254) | ||
| Sequences: | 1629 (1131.2) | ||
| Seq/Len: | 6.413 | ||
| Nf(neff/√len): | 71.0 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.413).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 28_C | 242_Y | 3.663 | 1.00 |
| 120_R | 162_V | 3.461 | 1.00 |
| 28_C | 246_L | 3.340 | 1.00 |
| 54_T | 142_N | 3.102 | 1.00 |
| 31_E | 242_Y | 2.879 | 1.00 |
| 27_S | 242_Y | 2.867 | 1.00 |
| 95_R | 140_Q | 2.800 | 1.00 |
| 53_A | 114_L | 2.776 | 1.00 |
| 120_R | 123_D | 2.515 | 1.00 |
| 28_C | 245_G | 2.497 | 1.00 |
| 239_K | 243_R | 2.363 | 1.00 |
| 27_S | 66_G | 2.272 | 1.00 |
| 9_N | 178_L | 2.182 | 1.00 |
| 95_R | 99_L | 2.105 | 1.00 |
| 233_V | 236_E | 2.099 | 1.00 |
| 9_N | 42_H | 2.025 | 1.00 |
| 44_Y | 178_L | 1.928 | 1.00 |
| 51_L | 142_N | 1.923 | 1.00 |
| 50_A | 145_S | 1.866 | 1.00 |
| 242_Y | 246_L | 1.812 | 1.00 |
| 126_N | 171_D | 1.811 | 1.00 |
| 118_L | 129_I | 1.788 | 1.00 |
| 14_G | 172_N | 1.778 | 1.00 |
| 28_C | 31_E | 1.760 | 1.00 |
| 70_I | 238_C | 1.743 | 1.00 |
| 173_L | 184_V | 1.728 | 1.00 |
| 11_S | 178_L | 1.724 | 1.00 |
| 9_N | 44_Y | 1.681 | 1.00 |
| 32_H | 253_G | 1.644 | 1.00 |
| 35_F | 253_G | 1.643 | 1.00 |
| 21_Y | 244_D | 1.623 | 1.00 |
| 122_S | 162_V | 1.609 | 1.00 |
| 70_I | 235_R | 1.571 | 0.99 |
| 13_A | 173_L | 1.542 | 0.99 |
| 20_V | 52_T | 1.536 | 0.99 |
| 241_G | 245_G | 1.534 | 0.99 |
| 129_I | 164_Y | 1.529 | 0.99 |
| 63_G | 67_A | 1.486 | 0.99 |
| 32_H | 249_L | 1.466 | 0.99 |
| 105_D | 108_E | 1.449 | 0.99 |
| 31_E | 246_L | 1.442 | 0.99 |
| 46_A | 117_S | 1.431 | 0.99 |
| 235_R | 239_K | 1.427 | 0.99 |
| 19_G | 48_A | 1.418 | 0.99 |
| 234_L | 238_C | 1.402 | 0.98 |
| 32_H | 35_F | 1.401 | 0.98 |
| 151_Y | 155_I | 1.387 | 0.98 |
| 95_R | 143_V | 1.383 | 0.98 |
| 8_W | 40_A | 1.382 | 0.98 |
| 116_I | 142_N | 1.370 | 0.98 |
| 25_V | 185_S | 1.351 | 0.98 |
| 98_L | 139_I | 1.328 | 0.98 |
| 171_D | 177_E | 1.315 | 0.97 |
| 125_E | 162_V | 1.313 | 0.97 |
| 171_D | 176_Y | 1.306 | 0.97 |
| 43_I | 53_A | 1.299 | 0.97 |
| 30_R | 56_L | 1.295 | 0.97 |
| 46_A | 142_N | 1.294 | 0.97 |
| 236_E | 240_Q | 1.288 | 0.97 |
| 122_S | 163_R | 1.279 | 0.97 |
| 183_T | 221_R | 1.278 | 0.97 |
| 120_R | 164_Y | 1.271 | 0.97 |
| 69_I | 98_L | 1.267 | 0.97 |
| 8_W | 181_T | 1.257 | 0.96 |
| 117_S | 126_N | 1.255 | 0.96 |
| 105_D | 109_R | 1.244 | 0.96 |
| 50_A | 146_T | 1.242 | 0.96 |
| 10_I | 25_V | 1.240 | 0.96 |
| 95_R | 139_I | 1.239 | 0.96 |
| 30_R | 59_G | 1.238 | 0.96 |
| 227_F | 244_D | 1.238 | 0.96 |
| 26_A | 56_L | 1.236 | 0.96 |
| 60_A | 68_N | 1.213 | 0.95 |
| 99_L | 139_I | 1.213 | 0.95 |
| 94_I | 143_V | 1.194 | 0.95 |
| 71_E | 235_R | 1.190 | 0.95 |
| 240_Q | 243_R | 1.177 | 0.94 |
| 129_I | 159_L | 1.175 | 0.94 |
| 28_C | 32_H | 1.164 | 0.94 |
| 127_V | 164_Y | 1.155 | 0.94 |
| 165_V | 169_I | 1.145 | 0.93 |
| 51_L | 94_I | 1.145 | 0.93 |
| 50_A | 156_P | 1.140 | 0.93 |
| 73_S | 231_P | 1.139 | 0.93 |
| 10_I | 26_A | 1.133 | 0.93 |
| 11_S | 15_C | 1.131 | 0.93 |
| 140_Q | 159_L | 1.125 | 0.92 |
| 42_H | 178_L | 1.124 | 0.92 |
| 147_F | 158_T | 1.122 | 0.92 |
| 54_T | 206_R | 1.115 | 0.92 |
| 64_E | 67_A | 1.106 | 0.91 |
| 45_G | 183_T | 1.102 | 0.91 |
| 104_A | 135_K | 1.100 | 0.91 |
| 58_T | 106_C | 1.082 | 0.90 |
| 67_A | 247_R | 1.078 | 0.90 |
| 28_C | 249_L | 1.077 | 0.90 |
| 24_G | 47_S | 1.073 | 0.90 |
| 181_T | 193_I | 1.069 | 0.90 |
| 142_N | 145_S | 1.069 | 0.90 |
| 67_A | 71_E | 1.056 | 0.89 |
| 243_R | 250_R | 1.054 | 0.89 |
| 19_G | 172_N | 1.043 | 0.88 |
| 53_A | 116_I | 1.042 | 0.88 |
| 127_V | 136_D | 1.041 | 0.88 |
| 25_V | 29_L | 1.036 | 0.87 |
| 68_N | 71_E | 1.033 | 0.87 |
| 14_G | 24_G | 1.022 | 0.87 |
| 88_F | 106_C | 1.021 | 0.86 |
| 119_T | 170_S | 1.019 | 0.86 |
| 32_H | 246_L | 1.019 | 0.86 |
| 120_R | 127_V | 1.013 | 0.86 |
| 107_H | 134_S | 1.010 | 0.86 |
| 20_V | 237_M | 1.008 | 0.85 |
| 159_L | 164_Y | 1.007 | 0.85 |
| 7_K | 41_T | 1.006 | 0.85 |
| 107_H | 110_A | 1.006 | 0.85 |
| 25_V | 245_G | 1.005 | 0.85 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4qmkA | 2 | 0.79 | 100 | 0.541 | Contact Map |
| 4akfA | 1 | 0.98 | 100 | 0.549 | Contact Map |
| 3tu3B | 1 | 0.82 | 100 | 0.553 | Contact Map |
| 4pk9A | 1 | 0.9 | 100 | 0.595 | Contact Map |
| 1cjyA | 2 | 0.7433 | 99.1 | 0.833 | Contact Map |
| 2qc3A | 1 | 0.77 | 91.4 | 0.931 | Contact Map |
| 4qbuA | 1 | 0.7967 | 91.3 | 0.931 | Contact Map |
| 4rr5A | 1 | 0 | 81.3 | 0.94 | Contact Map |
| 3im9A | 1 | 0.79 | 75 | 0.944 | Contact Map |
| 1nm2A | 1 | 0.7633 | 71.5 | 0.945 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)