| INN VH VL |
| ID: | 1494161302 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 225 (217) | ||
| Sequences: | 7704 (5287.2) | ||
| Seq/Len: | 35.502 | ||
| Nf(neff/√len): | 358.9 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 35.502).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 12_A | 86_L | 4.723 | 1.00 |
| 131_A | 196_L | 3.458 | 1.00 |
| 141_C | 206_C | 3.201 | 1.00 |
| 67_K | 90_D | 3.034 | 1.00 |
| 154_Y | 207_Q | 2.972 | 1.00 |
| 13_K | 16_A | 2.687 | 1.00 |
| 22_C | 96_C | 2.391 | 1.00 |
| 19_K | 82_E | 2.373 | 1.00 |
| 145_Q | 149_N | 2.372 | 1.00 |
| 18_V | 114_V | 2.296 | 1.00 |
| 20_V | 114_V | 2.247 | 1.00 |
| 155_Q | 202_A | 2.222 | 1.00 |
| 37_V | 97_A | 2.220 | 1.00 |
| 179_R | 200_D | 2.178 | 1.00 |
| 17_S | 84_S | 2.153 | 1.00 |
| 131_A | 137_V | 2.151 | 1.00 |
| 26_G | 32_F | 2.145 | 1.00 |
| 151_L | 189_Y | 2.135 | 1.00 |
| 14_P | 88_S | 2.101 | 1.00 |
| 17_S | 82_E | 2.090 | 1.00 |
| 138_T | 190_T | 2.032 | 1.00 |
| 93_V | 113_T | 1.999 | 1.00 |
| 155_Q | 204_Y | 1.958 | 1.00 |
| 138_T | 192_T | 1.924 | 1.00 |
| 143_A | 151_L | 1.914 | 1.00 |
| 136_R | 194_S | 1.902 | 1.00 |
| 36_W | 81_M | 1.880 | 1.00 |
| 132_S | 135_D | 1.866 | 1.00 |
| 152_A | 209_G | 1.839 | 1.00 |
| 38_R | 92_A | 1.799 | 1.00 |
| 154_Y | 205_Y | 1.771 | 1.00 |
| 28_T | 32_F | 1.731 | 1.00 |
| 24_A | 34_M | 1.706 | 1.00 |
| 37_V | 95_Y | 1.679 | 1.00 |
| 21_S | 80_Y | 1.664 | 1.00 |
| 17_S | 85_S | 1.661 | 1.00 |
| 136_R | 192_T | 1.625 | 1.00 |
| 100_L | 103_G | 1.584 | 1.00 |
| 95_Y | 111_G | 1.561 | 1.00 |
| 140_T | 190_T | 1.555 | 1.00 |
| 71_T | 82_E | 1.529 | 1.00 |
| 150_Y | 209_G | 1.523 | 1.00 |
| 12_A | 18_V | 1.522 | 1.00 |
| 93_V | 111_G | 1.503 | 1.00 |
| 19_K | 80_Y | 1.477 | 1.00 |
| 131_A | 135_D | 1.470 | 1.00 |
| 38_R | 94_Y | 1.447 | 1.00 |
| 21_S | 78_T | 1.443 | 1.00 |
| 143_A | 208_Q | 1.429 | 1.00 |
| 22_C | 141_C | 1.428 | 1.00 |
| 79_A | 189_Y | 1.417 | 1.00 |
| 156_Q | 159_G | 1.353 | 0.99 |
| 12_A | 16_A | 1.335 | 0.99 |
| 10_E | 18_V | 1.322 | 0.99 |
| 35_H | 97_A | 1.299 | 0.99 |
| 137_V | 193_I | 1.296 | 0.99 |
| 180_F | 193_I | 1.291 | 0.99 |
| 209_G | 214_Y | 1.290 | 0.99 |
| 34_M | 79_A | 1.259 | 0.99 |
| 142_R | 188_D | 1.255 | 0.99 |
| 122_M | 143_A | 1.250 | 0.99 |
| 32_F | 98_S | 1.240 | 0.98 |
| 68_A | 83_L | 1.240 | 0.98 |
| 99_F | 104_A | 1.239 | 0.98 |
| 39_Q | 95_Y | 1.234 | 0.98 |
| 193_I | 200_D | 1.233 | 0.98 |
| 140_T | 188_D | 1.215 | 0.98 |
| 35_H | 99_F | 1.214 | 0.98 |
| 137_V | 196_L | 1.207 | 0.98 |
| 11_L | 117_S | 1.181 | 0.98 |
| 156_Q | 162_P | 1.178 | 0.98 |
| 155_Q | 200_D | 1.169 | 0.97 |
| 152_A | 207_Q | 1.158 | 0.97 |
| 9_G | 112_T | 1.157 | 0.97 |
| 10_E | 20_V | 1.142 | 0.97 |
| 18_V | 83_L | 1.141 | 0.97 |
| 64_F | 68_A | 1.140 | 0.97 |
| 24_A | 98_S | 1.138 | 0.97 |
| 193_I | 196_L | 1.136 | 0.97 |
| 23_K | 78_T | 1.129 | 0.97 |
| 120_I | 210_N | 1.125 | 0.97 |
| 122_M | 125_S | 1.123 | 0.97 |
| 99_F | 102_R | 1.088 | 0.96 |
| 156_Q | 205_Y | 1.069 | 0.95 |
| 147_I | 208_Q | 1.066 | 0.95 |
| 91_T | 116_V | 1.063 | 0.95 |
| 217_G | 220_T | 1.047 | 0.94 |
| 136_R | 195_S | 1.039 | 0.94 |
| 181_S | 194_S | 1.032 | 0.94 |
| 156_Q | 161_A | 1.029 | 0.94 |
| 13_K | 117_S | 1.024 | 0.93 |
| 154_Y | 161_A | 1.021 | 0.93 |
| 122_M | 126_P | 1.019 | 0.93 |
| 10_E | 21_S | 1.016 | 0.93 |
| 129_L | 137_V | 1.012 | 0.93 |
| 18_V | 86_L | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1nqbA | 2 | 0.9956 | 100 | 0.151 | Contact Map |
| 3umtA | 1 | 1 | 100 | 0.154 | Contact Map |
| 1f3rB | 1 | 0.9911 | 100 | 0.168 | Contact Map |
| 2ghwB | 2 | 0.9956 | 100 | 0.171 | Contact Map |
| 5a2jH | 1 | 0 | 100 | 0.172 | Contact Map |
| 2gkiA | 2 | 1 | 100 | 0.175 | Contact Map |
| 3uzqA | 1 | 0.9822 | 100 | 0.176 | Contact Map |
| 4buhA | 2 | 0.9911 | 100 | 0.177 | Contact Map |
| 3juyB | 6 | 1 | 100 | 0.18 | Contact Map |
| 3gkzA | 1 | 0.9867 | 100 | 0.182 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)