| NLS1-MFSD2A |
| ID: | 1492992281 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 50% gaps before running GREMLIN. |
| Length: | 543 (449) | ||
| Sequences: | 6610 (4688) | ||
| Seq/Len: | 14.722 | ||
| Nf(neff/√len): | 221.2 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 14.722).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 48_C | 276_G | 3.895 | 1.00 |
| 105_W | 169_T | 3.431 | 1.00 |
| 109_T | 172_T | 3.030 | 1.00 |
| 120_S | 131_W | 2.864 | 1.00 |
| 466_A | 482_T | 2.617 | 1.00 |
| 369_K | 421_D | 2.606 | 1.00 |
| 511_R | 514_Q | 2.605 | 1.00 |
| 94_S | 157_T | 2.544 | 1.00 |
| 299_R | 303_S | 2.489 | 1.00 |
| 428_L | 518_A | 2.485 | 1.00 |
| 140_V | 267_I | 2.463 | 1.00 |
| 395_I | 399_A | 2.452 | 1.00 |
| 100_F | 457_G | 2.444 | 1.00 |
| 427_H | 523_R | 2.435 | 1.00 |
| 113_V | 116_C | 2.397 | 1.00 |
| 46_K | 194_S | 2.353 | 1.00 |
| 362_W | 365_T | 2.282 | 1.00 |
| 295_F | 299_R | 2.238 | 1.00 |
| 110_D | 449_K | 2.211 | 1.00 |
| 271_V | 275_L | 2.208 | 1.00 |
| 142_A | 166_L | 2.201 | 1.00 |
| 363_F | 372_A | 2.164 | 1.00 |
| 43_V | 47_L | 2.141 | 1.00 |
| 514_Q | 518_A | 2.121 | 1.00 |
| 135_S | 174_F | 2.114 | 1.00 |
| 128_L | 184_F | 2.102 | 1.00 |
| 73_D | 468_Y | 2.046 | 1.00 |
| 500_L | 504_M | 2.011 | 1.00 |
| 381_V | 492_I | 2.004 | 1.00 |
| 427_H | 435_G | 1.986 | 1.00 |
| 306_P | 506_P | 1.976 | 1.00 |
| 117_I | 128_L | 1.973 | 1.00 |
| 133_I | 274_I | 1.955 | 1.00 |
| 512_R | 516_K | 1.940 | 1.00 |
| 97_I | 101_V | 1.938 | 1.00 |
| 144_F | 260_G | 1.929 | 1.00 |
| 379_S | 407_S | 1.927 | 1.00 |
| 45_N | 280_Q | 1.924 | 1.00 |
| 457_G | 461_L | 1.912 | 1.00 |
| 68_Q | 460_T | 1.910 | 1.00 |
| 517_K | 521_A | 1.899 | 1.00 |
| 105_W | 109_T | 1.896 | 1.00 |
| 71_L | 163_F | 1.888 | 1.00 |
| 293_A | 296_R | 1.886 | 1.00 |
| 113_V | 172_T | 1.882 | 1.00 |
| 363_F | 367_F | 1.859 | 1.00 |
| 518_A | 522_L | 1.823 | 1.00 |
| 135_S | 173_C | 1.819 | 1.00 |
| 142_A | 170_M | 1.819 | 1.00 |
| 370_K | 374_Y | 1.803 | 1.00 |
| 334_Y | 468_Y | 1.803 | 1.00 |
| 113_V | 173_C | 1.773 | 1.00 |
| 253_K | 257_L | 1.769 | 1.00 |
| 508_D | 511_R | 1.761 | 1.00 |
| 477_E | 481_F | 1.755 | 1.00 |
| 381_V | 499_L | 1.733 | 1.00 |
| 93_F | 464_D | 1.730 | 1.00 |
| 305_G | 309_K | 1.723 | 1.00 |
| 476_P | 479_V | 1.722 | 1.00 |
| 72_L | 330_L | 1.688 | 1.00 |
| 496_L | 500_L | 1.687 | 1.00 |
| 190_T | 365_T | 1.680 | 1.00 |
| 157_T | 161_L | 1.654 | 1.00 |
| 125_L | 274_I | 1.649 | 1.00 |
| 98_I | 161_L | 1.640 | 1.00 |
| 374_Y | 499_L | 1.639 | 1.00 |
| 485_M | 489_M | 1.631 | 1.00 |
| 312_T | 501_L | 1.631 | 1.00 |
| 368_G | 371_T | 1.631 | 1.00 |
| 46_K | 191_E | 1.627 | 1.00 |
| 338_F | 341_E | 1.610 | 1.00 |
| 120_S | 123_T | 1.608 | 1.00 |
| 481_F | 485_M | 1.598 | 1.00 |
| 383_F | 400_V | 1.587 | 1.00 |
| 110_D | 172_T | 1.586 | 1.00 |
| 333_T | 339_R | 1.584 | 1.00 |
| 123_T | 130_P | 1.579 | 1.00 |
| 296_R | 299_R | 1.548 | 1.00 |
| 68_Q | 95_A | 1.542 | 1.00 |
| 272_I | 276_G | 1.533 | 1.00 |
| 97_I | 461_L | 1.531 | 1.00 |
| 367_F | 375_V | 1.527 | 1.00 |
| 418_M | 505_Y | 1.524 | 1.00 |
| 383_F | 407_S | 1.524 | 1.00 |
| 305_G | 506_P | 1.514 | 1.00 |
| 359_I | 362_W | 1.511 | 1.00 |
| 426_F | 436_T | 1.501 | 1.00 |
| 256_L | 260_G | 1.500 | 1.00 |
| 386_L | 400_V | 1.486 | 1.00 |
| 50_A | 202_V | 1.484 | 1.00 |
| 42_S | 45_N | 1.468 | 1.00 |
| 475_Q | 479_V | 1.467 | 1.00 |
| 214_Q | 262_I | 1.466 | 1.00 |
| 469_Q | 472_G | 1.462 | 1.00 |
| 368_G | 372_A | 1.448 | 1.00 |
| 428_L | 515_N | 1.437 | 1.00 |
| 138_L | 170_M | 1.433 | 1.00 |
| 76_Q | 91_G | 1.420 | 1.00 |
| 252_Q | 256_L | 1.414 | 1.00 |
| 506_P | 511_R | 1.413 | 1.00 |
| 335_T | 480_K | 1.410 | 1.00 |
| 145_L | 163_F | 1.398 | 0.99 |
| 268_I | 272_I | 1.397 | 0.99 |
| 53_G | 199_R | 1.393 | 0.99 |
| 316_F | 501_L | 1.392 | 0.99 |
| 114_G | 438_P | 1.388 | 0.99 |
| 518_A | 521_A | 1.388 | 0.99 |
| 46_K | 198_Y | 1.385 | 0.99 |
| 107_A | 446_F | 1.377 | 0.99 |
| 106_D | 449_K | 1.377 | 0.99 |
| 257_L | 260_G | 1.369 | 0.99 |
| 309_K | 504_M | 1.368 | 0.99 |
| 118_S | 438_P | 1.364 | 0.99 |
| 489_M | 493_V | 1.363 | 0.99 |
| 158_Y | 162_L | 1.354 | 0.99 |
| 517_K | 520_Q | 1.346 | 0.99 |
| 188_E | 191_E | 1.343 | 0.99 |
| 316_F | 497_L | 1.343 | 0.99 |
| 148_F | 256_L | 1.338 | 0.99 |
| 103_R | 106_D | 1.331 | 0.99 |
| 214_Q | 258_A | 1.328 | 0.99 |
| 386_L | 390_M | 1.326 | 0.99 |
| 43_V | 46_K | 1.321 | 0.99 |
| 376_G | 410_A | 1.317 | 0.99 |
| 507_I | 512_R | 1.316 | 0.99 |
| 374_Y | 503_K | 1.314 | 0.99 |
| 520_Q | 524_D | 1.311 | 0.99 |
| 155_G | 158_Y | 1.307 | 0.99 |
| 369_K | 417_S | 1.306 | 0.99 |
| 54_A | 206_G | 1.298 | 0.99 |
| 73_D | 333_T | 1.292 | 0.99 |
| 362_W | 366_R | 1.291 | 0.99 |
| 481_F | 484_N | 1.284 | 0.99 |
| 500_L | 503_K | 1.268 | 0.99 |
| 135_S | 170_M | 1.266 | 0.99 |
| 308_I | 312_T | 1.263 | 0.99 |
| 51_L | 272_I | 1.262 | 0.99 |
| 109_T | 169_T | 1.255 | 0.99 |
| 466_A | 479_V | 1.249 | 0.99 |
| 186_S | 191_E | 1.248 | 0.99 |
| 516_K | 520_Q | 1.246 | 0.98 |
| 43_V | 198_Y | 1.234 | 0.98 |
| 260_G | 264_C | 1.233 | 0.98 |
| 52_G | 178_Y | 1.229 | 0.98 |
| 117_I | 184_F | 1.229 | 0.98 |
| 120_S | 130_P | 1.228 | 0.98 |
| 159_W | 163_F | 1.225 | 0.98 |
| 313_G | 502_F | 1.224 | 0.98 |
| 125_L | 134_F | 1.220 | 0.98 |
| 307_Y | 440_F | 1.210 | 0.98 |
| 55_P | 269_C | 1.207 | 0.98 |
| 480_K | 484_N | 1.188 | 0.98 |
| 367_F | 372_A | 1.182 | 0.98 |
| 513_R | 517_K | 1.179 | 0.98 |
| 112_L | 116_C | 1.175 | 0.98 |
| 479_V | 482_T | 1.173 | 0.98 |
| 257_L | 261_V | 1.171 | 0.98 |
| 393_N | 396_I | 1.168 | 0.97 |
| 512_R | 515_N | 1.167 | 0.97 |
| 129_M | 279_E | 1.166 | 0.97 |
| 129_M | 277_V | 1.166 | 0.97 |
| 428_L | 522_L | 1.163 | 0.97 |
| 94_S | 161_L | 1.161 | 0.97 |
| 306_P | 425_D | 1.157 | 0.97 |
| 129_M | 274_I | 1.156 | 0.97 |
| 352_S | 402_V | 1.155 | 0.97 |
| 129_M | 133_I | 1.147 | 0.97 |
| 48_C | 272_I | 1.145 | 0.97 |
| 96_S | 460_T | 1.143 | 0.97 |
| 485_M | 493_V | 1.141 | 0.97 |
| 125_L | 133_I | 1.139 | 0.97 |
| 106_D | 172_T | 1.133 | 0.97 |
| 493_V | 497_L | 1.128 | 0.97 |
| 341_E | 344_N | 1.124 | 0.97 |
| 40_Q | 191_E | 1.122 | 0.97 |
| 144_F | 148_F | 1.121 | 0.97 |
| 361_Q | 365_T | 1.121 | 0.97 |
| 69_I | 74_V | 1.121 | 0.97 |
| 186_S | 195_A | 1.119 | 0.96 |
| 462_S | 486_L | 1.117 | 0.96 |
| 427_H | 522_L | 1.112 | 0.96 |
| 93_F | 96_S | 1.112 | 0.96 |
| 135_S | 138_L | 1.109 | 0.96 |
| 343_Q | 346_L | 1.109 | 0.96 |
| 511_R | 515_N | 1.103 | 0.96 |
| 56_Y | 175_H | 1.100 | 0.96 |
| 50_A | 198_Y | 1.095 | 0.96 |
| 378_S | 499_L | 1.092 | 0.96 |
| 71_L | 160_Y | 1.083 | 0.96 |
| 521_A | 524_D | 1.080 | 0.95 |
| 132_I | 181_L | 1.080 | 0.95 |
| 467_G | 470_T | 1.080 | 0.95 |
| 394_L | 398_Y | 1.077 | 0.95 |
| 305_G | 308_I | 1.075 | 0.95 |
| 40_Q | 188_E | 1.073 | 0.95 |
| 253_K | 256_L | 1.072 | 0.95 |
| 477_E | 480_K | 1.071 | 0.95 |
| 190_T | 509_E | 1.071 | 0.95 |
| 69_I | 333_T | 1.068 | 0.95 |
| 304_H | 426_F | 1.067 | 0.95 |
| 311_I | 447_F | 1.064 | 0.95 |
| 376_G | 407_S | 1.061 | 0.95 |
| 92_P | 464_D | 1.060 | 0.95 |
| 478_R | 481_F | 1.060 | 0.95 |
| 54_A | 205_L | 1.060 | 0.95 |
| 98_I | 157_T | 1.059 | 0.95 |
| 356_T | 406_I | 1.058 | 0.95 |
| 92_P | 460_T | 1.058 | 0.95 |
| 520_Q | 523_R | 1.056 | 0.95 |
| 492_I | 496_L | 1.055 | 0.95 |
| 106_D | 110_D | 1.053 | 0.95 |
| 250_E | 253_K | 1.053 | 0.95 |
| 136_T | 140_V | 1.053 | 0.95 |
| 474_S | 477_E | 1.051 | 0.94 |
| 515_N | 519_L | 1.049 | 0.94 |
| 299_R | 302_M | 1.038 | 0.94 |
| 59_T | 167_F | 1.037 | 0.94 |
| 264_C | 268_I | 1.037 | 0.94 |
| 327_N | 459_S | 1.033 | 0.94 |
| 73_D | 334_Y | 1.031 | 0.94 |
| 423_I | 441_F | 1.027 | 0.94 |
| 91_G | 94_S | 1.026 | 0.94 |
| 53_G | 178_Y | 1.022 | 0.93 |
| 182_T | 441_F | 1.018 | 0.93 |
| 492_I | 499_L | 1.017 | 0.93 |
| 316_F | 494_L | 1.016 | 0.93 |
| 509_E | 513_R | 1.011 | 0.93 |
| 519_L | 523_R | 1.011 | 0.93 |
| 117_I | 177_P | 1.010 | 0.93 |
| 93_F | 97_I | 1.005 | 0.93 |
| 319_L | 494_L | 1.005 | 0.93 |
| 102_G | 165_C | 1.002 | 0.92 |
| 182_T | 196_T | 1.002 | 0.92 |
| 367_F | 371_T | 1.001 | 0.92 |
| 142_A | 146_I | 1.001 | 0.92 |
| 203_E | 207_T | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4m64A | 4 | 0.8122 | 100 | 0.49 | Contact Map |
| 3wdoA | 1 | 0.7772 | 100 | 0.652 | Contact Map |
| 1pw4A | 1 | 0.7643 | 100 | 0.666 | Contact Map |
| 4j05A | 2 | 0.7403 | 100 | 0.673 | Contact Map |
| 2cfqA | 1 | 0.744 | 100 | 0.676 | Contact Map |
| 3o7qA | 1 | 0.7551 | 100 | 0.68 | Contact Map |
| 4tphA | 2 | 0.7182 | 100 | 0.683 | Contact Map |
| 4u4vA | 1 | 0 | 100 | 0.685 | Contact Map |
| 4pypA | 1 | 0.7164 | 100 | 0.686 | Contact Map |
| 4w6vA | 1 | 0 | 100 | 0.69 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)