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OPENSEQ.org

MLKL

ID: 1490793830 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 471 (465)
Sequences: 503 (438.1)
Seq/Len: 1.082
Nf(neff/√len): 20.3

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.082).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
331_K336_N2.3680.99
317_L459_V2.0890.97
333_R336_N2.0860.97
333_R349_G2.0740.97
395_I459_V2.0380.97
291_L295_L2.0250.96
312_G345_V2.0210.96
284_E291_L1.9430.95
459_V462_I1.9330.95
284_E346_K1.9140.95
448_C457_P1.9110.95
316_G347_L1.9060.95
447_E465_K1.8780.94
25_K31_L1.8240.93
310_V402_L1.8130.92
313_A331_K1.7730.91
376_Y449_R1.7510.91
227_V230_K1.7470.90
258_E261_N1.7360.90
294_L343_Y1.7350.90
292_R334_S1.7350.90
339_V345_V1.6840.88
259_S319_R1.6830.88
263_L348_A1.6490.87
340_T344_Q1.6370.86
404_E411_P1.6340.86
210_R217_L1.6250.86
309_L339_V1.6210.85
21_M25_K1.6170.85
314_A317_L1.6170.85
265_I281_I1.6070.85
217_L349_G1.6020.85
389_Y397_S1.5540.82
212_N349_G1.5400.81
294_L445_I1.5310.81
250_E351_E1.5260.80
76_I399_G1.5150.80
441_L448_C1.5110.79
212_N215_S1.5040.79
448_C462_I1.5030.79
337_F347_L1.4870.78
321_H348_A1.4730.77
347_L459_V1.4520.76
439_S442_R1.4360.74
337_F345_V1.4340.74
254_M330_G1.4220.73
313_A337_F1.4180.73
424_V430_Q1.4170.73
290_T319_R1.4150.73
445_I449_R1.4140.73
210_R333_R1.3990.72
317_L332_I1.3990.72
224_R257_F1.3950.71
317_L347_L1.3930.71
212_N331_K1.3900.71
433_L439_S1.3870.71
329_H350_F1.3870.71
230_K349_G1.3800.70
310_V405_I1.3740.70
400_I442_R1.3730.70
456_R466_L1.3680.69
453_P456_R1.3650.69
333_R396_Y1.3580.68
321_H329_H1.3560.68
450_A455_V1.3520.68
333_R372_K1.3490.68
378_S412_F1.3480.68
76_I211_E1.3420.67
250_E331_K1.3390.67
68_A397_S1.3340.66
331_K349_G1.3280.66
264_R284_E1.3180.65
430_Q449_R1.3130.65
259_S310_V1.3110.64
433_L442_R1.3060.64
290_T294_L1.3000.63
406_A441_L1.2960.63
266_F280_S1.2940.63
452_D455_V1.2940.63
290_T293_E1.2920.63
217_L333_R1.2890.63
316_G320_L1.2860.62
254_M442_R1.2830.62
291_L339_V1.2820.62
334_S376_Y1.2810.62
85_I317_L1.2780.62
160_A462_I1.2740.61
213_E349_G1.2740.61
71_E104_K1.2580.60
320_L329_H1.2450.59
317_L398_F1.2450.59
220_G247_F1.2410.58
376_Y405_I1.2380.58
34_R291_L1.2340.58
28_C35_V1.2340.58
262_I337_F1.2310.57
376_Y396_Y1.2300.57
431_E449_R1.2280.57
291_L334_S1.2250.57
210_R349_G1.2240.57
83_S145_R1.2220.56
76_I79_F1.2170.56
254_M339_V1.2140.56
378_S453_P1.2120.55
62_M65_F1.2100.55
259_S372_K1.2080.55
424_V428_R1.2050.55
333_R373_S1.2030.55
399_G402_L1.2020.55
82_R285_Y1.1920.54
430_Q459_V1.1920.54
412_F449_R1.1890.53
331_K373_S1.1890.53
254_M400_I1.1880.53
261_N314_A1.1870.53
340_T448_C1.1810.53
306_R408_G1.1800.52
210_R213_E1.1780.52
209_L217_L1.1760.52
144_D462_I1.1750.52
254_M294_L1.1690.51
334_S339_V1.1680.51
258_E347_L1.1680.51
173_K179_Y1.1670.51
332_I376_Y1.1640.51
258_E264_R1.1630.51
380_Q404_E1.1610.51
334_S401_V1.1490.50
257_F351_E1.1490.50
10_L32_G1.1470.49
88_F94_D1.1420.49
333_R378_S1.1400.49
212_N338_L1.1400.49
253_T350_F1.1360.48
313_A347_L1.1350.48
101_V376_Y1.1340.48
16_K398_F1.1320.48
343_Y442_R1.1320.48
6_H10_L1.1310.48
201_L334_S1.1310.48
87_R290_T1.1290.48
395_I398_F1.1270.47
294_L316_G1.1270.47
254_M334_S1.1260.47
349_G372_K1.1200.47
11_G21_M1.1180.47
180_L183_K1.1170.46
279_F400_I1.1160.46
310_V441_L1.1150.46
398_F402_L1.1140.46
212_N217_L1.1100.46
10_L18_C1.1090.46
88_F408_G1.1080.46
331_K372_K1.1070.46
80_S265_I1.1060.45
329_H336_N1.1000.45
128_S131_A1.0980.45
245_Q347_L1.0920.44
286_C351_E1.0900.44
430_Q446_D1.0860.44
393_S397_S1.0860.44
267_G282_V1.0850.43
212_N336_N1.0850.43
262_I316_G1.0830.43
334_S375_A1.0820.43
444_I469_F1.0780.43
401_V433_L1.0760.43
150_M262_I1.0740.42
291_L401_V1.0720.42
68_A104_K1.0710.42
257_F319_R1.0620.41
376_Y400_I1.0620.41
67_A70_E1.0590.41
109_W112_L1.0590.41
444_I448_C1.0560.41
212_N372_K1.0540.41
217_L220_G1.0530.41
430_Q451_H1.0520.40
310_V403_W1.0500.40
83_S252_K1.0500.40
152_R179_Y1.0490.40
69_L95_K1.0480.40
210_R215_S1.0480.40
426_V455_V1.0470.40
261_N264_R1.0460.40
261_N398_F1.0440.40
406_A448_C1.0430.40
253_T436_D1.0430.40
20_E23_Y1.0430.40
15_H22_K1.0420.40
169_M397_S1.0410.39
334_S405_I1.0390.39
162_L353_R1.0390.39
35_V98_F1.0390.39
334_S438_P1.0340.39
217_L230_K1.0310.39
330_G373_S1.0310.39
322_H328_L1.0290.38
194_K268_I1.0290.38
291_L466_L1.0270.38
28_C253_T1.0240.38
32_G36_L1.0230.38
389_Y403_W1.0220.38
84_N88_F1.0210.38
10_L20_E1.0200.38
228_A285_Y1.0200.38
375_A439_S1.0190.37
314_A321_H1.0180.37
321_H394_E1.0150.37
217_L372_K1.0140.37
377_L393_S1.0140.37
223_H339_V1.0140.37
222_Y438_P1.0130.37
246_T284_E1.0130.37
12_Q16_K1.0110.37
205_P332_I1.0110.37
254_M401_V1.0110.37
213_E217_L1.0080.36
291_L442_R1.0050.36
330_G400_I1.0050.36
212_N351_E1.0030.36
321_H448_C1.0030.36
102_N105_L1.0020.36
230_K353_R1.0020.36
46_Q399_G1.0010.36
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4btfA10.87471000.372Contact Map
4gt4A20.56051000.505Contact Map
4aszA10.56261000.509Contact Map
5a46A201000.511Contact Map
4fyoA10.53931000.513Contact Map
4rt7A101000.514Contact Map
4aojA30.52871000.516Contact Map
4mwiA10.56481000.522Contact Map
4aseA10.6031000.522Contact Map
4otwA10.54141000.522Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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