| metarhizium |
| ID: | 1490000216 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 43 (41) | ||
| Sequences: | 46 (42) | ||
| Seq/Len: | 1.122 | ||
| Nf(neff/√len): | 6.6 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.122).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 21_Q | 30_Q | 2.254 | 0.99 |
| 7_N | 31_F | 1.822 | 0.93 |
| 29_N | 33_G | 1.724 | 0.90 |
| 14_L | 21_Q | 1.596 | 0.85 |
| 6_N | 41_Q | 1.548 | 0.82 |
| 8_Y | 17_P | 1.409 | 0.73 |
| 12_D | 25_K | 1.353 | 0.69 |
| 20_T | 37_S | 1.341 | 0.68 |
| 33_G | 36_F | 1.321 | 0.66 |
| 23_I | 30_Q | 1.313 | 0.66 |
| 4_S | 7_N | 1.252 | 0.60 |
| 33_G | 38_G | 1.204 | 0.56 |
| 10_S | 41_Q | 1.158 | 0.51 |
| 9_G | 27_S | 1.154 | 0.51 |
| 29_N | 43_G | 1.111 | 0.47 |
| 18_S | 27_S | 1.103 | 0.46 |
| 36_F | 42_F | 1.076 | 0.44 |
| 35_T | 38_G | 1.071 | 0.43 |
| 25_K | 41_Q | 1.069 | 0.43 |
| 20_T | 34_A | 1.065 | 0.43 |
| 11_G | 14_L | 1.064 | 0.42 |
| 26_G | 36_F | 1.049 | 0.41 |
| 17_P | 42_F | 1.040 | 0.40 |
| 16_A | 43_G | 1.022 | 0.39 |
| 19_G | 28_G | 1.005 | 0.37 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3fewX | 1 | 0 | 0.8 | 0.884 | Contact Map |
| 3rlfF | 1 | 1 | 0.8 | 0.886 | Contact Map |
| 4j94A | 1 | 1 | 0.8 | 0.886 | Contact Map |
| 2b6nA | 1 | 1 | 0.7 | 0.887 | Contact Map |
| 2wq4A | 3 | 0.093 | 0.7 | 0.892 | Contact Map |
| 4h6xA | 2 | 1 | 0.6 | 0.893 | Contact Map |
| 4mzdA | 1 | 0.9767 | 0.6 | 0.897 | Contact Map |
| 1sh7A | 2 | 1 | 0.6 | 0.897 | Contact Map |
| 1gciA | 1 | 1 | 0.5 | 0.9 | Contact Map |
| 1r6vA | 1 | 1 | 0.5 | 0.901 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)