| lntEco |
| ID: | 1488188808 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 512 (497) | ||
| Sequences: | 3379 (2847.6) | ||
| Seq/Len: | 6.799 | ||
| Nf(neff/√len): | 127.7 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.799).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 167_M | 171_A | 5.682 | 1.00 |
| 451_V | 472_T | 4.180 | 1.00 |
| 453_G | 457_E | 4.112 | 1.00 |
| 426_F | 450_A | 3.646 | 1.00 |
| 462_I | 468_E | 3.549 | 1.00 |
| 436_L | 484_A | 3.009 | 1.00 |
| 161_K | 434_L | 2.952 | 1.00 |
| 316_I | 410_I | 2.740 | 1.00 |
| 411_S | 444_T | 2.619 | 1.00 |
| 152_G | 169_V | 2.612 | 1.00 |
| 157_D | 430_R | 2.492 | 1.00 |
| 229_G | 249_Y | 2.413 | 1.00 |
| 314_N | 319_L | 2.396 | 1.00 |
| 407_L | 440_L | 2.189 | 1.00 |
| 432_R | 435_E | 2.182 | 1.00 |
| 247_K | 251_N | 2.161 | 1.00 |
| 245_T | 249_Y | 2.081 | 1.00 |
| 483_Y | 488_N | 2.080 | 1.00 |
| 225_S | 260_S | 2.046 | 1.00 |
| 209_R | 212_Q | 2.029 | 1.00 |
| 315_T | 334_N | 2.028 | 1.00 |
| 382_L | 406_Y | 1.972 | 1.00 |
| 71_G | 149_L | 1.920 | 1.00 |
| 298_G | 301_D | 1.912 | 1.00 |
| 249_Y | 252_A | 1.898 | 1.00 |
| 165_P | 434_L | 1.892 | 1.00 |
| 226_M | 443_S | 1.887 | 1.00 |
| 249_Y | 253_T | 1.876 | 1.00 |
| 248_I | 252_A | 1.865 | 1.00 |
| 245_T | 248_I | 1.846 | 1.00 |
| 164_A | 431_M | 1.845 | 1.00 |
| 225_S | 471_T | 1.840 | 1.00 |
| 423_W | 448_I | 1.837 | 1.00 |
| 288_L | 295_L | 1.798 | 1.00 |
| 440_L | 452_I | 1.793 | 1.00 |
| 51_R | 55_Q | 1.790 | 1.00 |
| 433_A | 440_L | 1.782 | 1.00 |
| 35_P | 38_I | 1.776 | 1.00 |
| 25_T | 70_S | 1.765 | 1.00 |
| 26_L | 31_Y | 1.761 | 1.00 |
| 252_A | 255_P | 1.749 | 1.00 |
| 292_G | 298_G | 1.726 | 1.00 |
| 20_F | 43_G | 1.721 | 1.00 |
| 439_P | 454_P | 1.715 | 1.00 |
| 18_L | 62_C | 1.713 | 1.00 |
| 164_A | 172_I | 1.705 | 1.00 |
| 348_E | 364_S | 1.702 | 1.00 |
| 226_M | 449_T | 1.697 | 1.00 |
| 253_T | 265_W | 1.680 | 1.00 |
| 224_V | 441_L | 1.667 | 1.00 |
| 365_F | 368_G | 1.664 | 1.00 |
| 52_R | 55_Q | 1.640 | 1.00 |
| 62_C | 66_G | 1.613 | 1.00 |
| 482_P | 486_T | 1.607 | 1.00 |
| 284_L | 288_L | 1.605 | 1.00 |
| 294_S | 298_G | 1.594 | 1.00 |
| 437_A | 481_T | 1.590 | 1.00 |
| 242_L | 245_T | 1.581 | 1.00 |
| 27_A | 37_A | 1.578 | 1.00 |
| 314_N | 317_I | 1.577 | 1.00 |
| 314_N | 318_T | 1.572 | 1.00 |
| 271_T | 274_E | 1.564 | 1.00 |
| 390_I | 440_L | 1.550 | 0.99 |
| 60_G | 64_G | 1.545 | 0.99 |
| 54_L | 57_A | 1.540 | 0.99 |
| 364_S | 368_G | 1.536 | 0.99 |
| 406_Y | 474_V | 1.532 | 0.99 |
| 24_G | 40_S | 1.532 | 0.99 |
| 453_G | 459_Q | 1.529 | 0.99 |
| 284_L | 287_E | 1.528 | 0.99 |
| 138_L | 142_V | 1.523 | 0.99 |
| 21_G | 24_G | 1.523 | 0.99 |
| 352_R | 359_D | 1.520 | 0.99 |
| 125_A | 129_P | 1.517 | 0.99 |
| 223_Q | 473_N | 1.500 | 0.99 |
| 298_G | 302_A | 1.498 | 0.99 |
| 417_G | 465_F | 1.497 | 0.99 |
| 449_T | 462_I | 1.490 | 0.99 |
| 385_A | 396_V | 1.479 | 0.99 |
| 94_V | 97_L | 1.466 | 0.99 |
| 153_Y | 431_M | 1.460 | 0.99 |
| 162_G | 165_P | 1.450 | 0.99 |
| 426_F | 458_I | 1.450 | 0.99 |
| 295_L | 298_G | 1.447 | 0.99 |
| 243_L | 247_K | 1.437 | 0.99 |
| 248_I | 253_T | 1.433 | 0.99 |
| 97_L | 101_L | 1.433 | 0.99 |
| 241_Q | 244_N | 1.428 | 0.99 |
| 165_P | 437_A | 1.428 | 0.99 |
| 189_K | 193_R | 1.426 | 0.99 |
| 460_A | 472_T | 1.424 | 0.99 |
| 439_P | 475_T | 1.416 | 0.99 |
| 285_D | 289_R | 1.414 | 0.99 |
| 100_Y | 104_Y | 1.406 | 0.99 |
| 337_H | 395_Q | 1.400 | 0.99 |
| 383_T | 407_L | 1.399 | 0.99 |
| 133_Q | 174_F | 1.398 | 0.99 |
| 78_S | 416_F | 1.391 | 0.99 |
| 321_K | 324_P | 1.389 | 0.99 |
| 98_A | 102_S | 1.387 | 0.99 |
| 253_T | 256_L | 1.387 | 0.99 |
| 347_L | 351_L | 1.377 | 0.98 |
| 207_P | 210_Y | 1.373 | 0.98 |
| 110_G | 114_R | 1.372 | 0.98 |
| 55_Q | 58_A | 1.367 | 0.98 |
| 244_N | 247_K | 1.367 | 0.98 |
| 94_V | 98_A | 1.361 | 0.98 |
| 273_L | 276_N | 1.350 | 0.98 |
| 97_L | 100_Y | 1.348 | 0.98 |
| 86_P | 90_N | 1.345 | 0.98 |
| 89_V | 92_F | 1.343 | 0.98 |
| 413_D | 425_H | 1.341 | 0.98 |
| 155_Q | 176_L | 1.330 | 0.98 |
| 426_F | 452_I | 1.323 | 0.98 |
| 61_F | 99_A | 1.319 | 0.98 |
| 316_I | 384_A | 1.318 | 0.98 |
| 315_T | 319_L | 1.317 | 0.98 |
| 101_L | 105_T | 1.313 | 0.98 |
| 244_N | 248_I | 1.304 | 0.98 |
| 230_D | 467_R | 1.304 | 0.98 |
| 151_F | 177_M | 1.289 | 0.97 |
| 61_F | 65_F | 1.287 | 0.97 |
| 137_F | 141_W | 1.273 | 0.97 |
| 60_G | 63_W | 1.266 | 0.97 |
| 245_T | 252_A | 1.265 | 0.97 |
| 480_L | 484_A | 1.262 | 0.97 |
| 348_E | 352_R | 1.257 | 0.97 |
| 499_G | 502_A | 1.254 | 0.97 |
| 87_G | 90_N | 1.254 | 0.97 |
| 208_L | 213_W | 1.254 | 0.97 |
| 123_R | 127_A | 1.250 | 0.97 |
| 226_M | 441_L | 1.248 | 0.97 |
| 429_A | 440_L | 1.246 | 0.97 |
| 264_I | 408_L | 1.242 | 0.97 |
| 458_I | 461_M | 1.241 | 0.96 |
| 92_F | 97_L | 1.240 | 0.96 |
| 280_F | 284_L | 1.237 | 0.96 |
| 93_L | 97_L | 1.237 | 0.96 |
| 294_S | 297_T | 1.236 | 0.96 |
| 225_S | 256_L | 1.231 | 0.96 |
| 271_T | 275_I | 1.228 | 0.96 |
| 383_T | 404_T | 1.227 | 0.96 |
| 97_L | 104_Y | 1.224 | 0.96 |
| 102_S | 105_T | 1.223 | 0.96 |
| 311_D | 314_N | 1.222 | 0.96 |
| 355_A | 358_F | 1.221 | 0.96 |
| 190_R | 193_R | 1.215 | 0.96 |
| 241_Q | 245_T | 1.213 | 0.96 |
| 211_I | 214_F | 1.206 | 0.96 |
| 122_L | 126_I | 1.201 | 0.96 |
| 250_Y | 253_T | 1.197 | 0.95 |
| 206_F | 210_Y | 1.196 | 0.95 |
| 300_V | 365_F | 1.194 | 0.95 |
| 33_V | 36_A | 1.194 | 0.95 |
| 92_F | 100_Y | 1.190 | 0.95 |
| 27_A | 154_S | 1.188 | 0.95 |
| 390_I | 409_T | 1.188 | 0.95 |
| 209_R | 213_W | 1.187 | 0.95 |
| 291_K | 294_S | 1.186 | 0.95 |
| 207_P | 211_I | 1.186 | 0.95 |
| 31_Y | 420_I | 1.186 | 0.95 |
| 83_G | 86_P | 1.183 | 0.95 |
| 24_G | 27_A | 1.175 | 0.95 |
| 235_L | 238_D | 1.173 | 0.95 |
| 89_V | 93_L | 1.173 | 0.95 |
| 288_L | 291_K | 1.169 | 0.95 |
| 50_N | 54_L | 1.168 | 0.95 |
| 43_G | 47_L | 1.161 | 0.94 |
| 35_P | 39_I | 1.156 | 0.94 |
| 246_L | 249_Y | 1.156 | 0.94 |
| 206_F | 209_R | 1.154 | 0.94 |
| 272_D | 275_I | 1.153 | 0.94 |
| 233_Q | 236_K | 1.152 | 0.94 |
| 80_A | 83_G | 1.150 | 0.94 |
| 59_I | 62_C | 1.149 | 0.94 |
| 211_I | 215_T | 1.145 | 0.94 |
| 248_I | 467_R | 1.143 | 0.94 |
| 253_T | 263_I | 1.142 | 0.94 |
| 45_Q | 48_T | 1.142 | 0.94 |
| 112_L | 115_L | 1.141 | 0.94 |
| 49_F | 52_R | 1.141 | 0.94 |
| 296_V | 408_L | 1.140 | 0.94 |
| 284_L | 289_R | 1.140 | 0.94 |
| 14_L | 62_C | 1.139 | 0.94 |
| 100_Y | 103_L | 1.137 | 0.94 |
| 480_L | 485_R | 1.136 | 0.93 |
| 100_Y | 105_T | 1.129 | 0.93 |
| 314_N | 320_G | 1.128 | 0.93 |
| 56_S | 59_I | 1.126 | 0.93 |
| 114_R | 118_K | 1.121 | 0.93 |
| 224_V | 406_Y | 1.115 | 0.93 |
| 202_F | 206_F | 1.113 | 0.93 |
| 64_G | 67_L | 1.112 | 0.93 |
| 163_L | 175_L | 1.112 | 0.93 |
| 374_P | 377_A | 1.110 | 0.92 |
| 101_L | 104_Y | 1.107 | 0.92 |
| 236_K | 268_S | 1.106 | 0.92 |
| 54_L | 58_A | 1.105 | 0.92 |
| 452_I | 458_I | 1.104 | 0.92 |
| 242_L | 249_Y | 1.104 | 0.92 |
| 282_K | 285_D | 1.103 | 0.92 |
| 275_I | 278_Q | 1.102 | 0.92 |
| 495_T | 499_G | 1.101 | 0.92 |
| 268_S | 445_N | 1.098 | 0.92 |
| 397_R | 487_G | 1.098 | 0.92 |
| 133_Q | 173_N | 1.093 | 0.92 |
| 226_M | 470_L | 1.090 | 0.92 |
| 246_L | 253_T | 1.090 | 0.92 |
| 167_M | 175_L | 1.090 | 0.92 |
| 87_G | 91_I | 1.089 | 0.91 |
| 104_Y | 108_F | 1.089 | 0.91 |
| 91_I | 94_V | 1.085 | 0.91 |
| 323_A | 327_Y | 1.083 | 0.91 |
| 153_Y | 428_M | 1.082 | 0.91 |
| 113_S | 117_P | 1.082 | 0.91 |
| 52_R | 56_S | 1.082 | 0.91 |
| 406_Y | 441_L | 1.082 | 0.91 |
| 138_L | 141_W | 1.078 | 0.91 |
| 22_A | 26_L | 1.078 | 0.91 |
| 320_G | 323_A | 1.078 | 0.91 |
| 77_V | 419_S | 1.076 | 0.91 |
| 235_L | 241_Q | 1.074 | 0.91 |
| 240_G | 244_N | 1.072 | 0.91 |
| 65_F | 69_G | 1.070 | 0.90 |
| 40_S | 63_W | 1.068 | 0.90 |
| 90_N | 94_V | 1.068 | 0.90 |
| 460_A | 470_L | 1.067 | 0.90 |
| 208_L | 212_Q | 1.067 | 0.90 |
| 249_Y | 256_L | 1.059 | 0.90 |
| 318_T | 321_K | 1.055 | 0.90 |
| 358_F | 361_P | 1.054 | 0.90 |
| 503_V | 508_R | 1.052 | 0.89 |
| 82_F | 86_P | 1.050 | 0.89 |
| 380_I | 405_D | 1.049 | 0.89 |
| 88_P | 91_I | 1.048 | 0.89 |
| 84_G | 87_G | 1.047 | 0.89 |
| 108_F | 112_L | 1.043 | 0.89 |
| 297_T | 301_D | 1.037 | 0.89 |
| 347_L | 350_I | 1.031 | 0.88 |
| 72_I | 146_F | 1.030 | 0.88 |
| 106_G | 109_A | 1.030 | 0.88 |
| 86_P | 91_I | 1.027 | 0.88 |
| 60_G | 106_G | 1.027 | 0.88 |
| 296_V | 299_I | 1.025 | 0.88 |
| 441_L | 451_V | 1.024 | 0.88 |
| 285_D | 288_L | 1.022 | 0.88 |
| 459_Q | 472_T | 1.022 | 0.88 |
| 281_L | 285_D | 1.021 | 0.88 |
| 57_A | 106_G | 1.020 | 0.87 |
| 114_R | 117_P | 1.020 | 0.87 |
| 306_K | 309_R | 1.016 | 0.87 |
| 295_L | 299_I | 1.015 | 0.87 |
| 226_M | 451_V | 1.012 | 0.87 |
| 413_D | 442_R | 1.011 | 0.87 |
| 73_N | 419_S | 1.007 | 0.87 |
| 401_R | 404_T | 1.004 | 0.86 |
| 400_F | 403_D | 1.004 | 0.86 |
| 42_M | 46_A | 1.001 | 0.86 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1uf5A | 2 | 0.5098 | 100 | 0.713 | Contact Map |
| 3p8kA | 2 | 0.4766 | 100 | 0.736 | Contact Map |
| 2w1vA | 2 | 0.4805 | 100 | 0.738 | Contact Map |
| 4lf0A | 1 | 0.5254 | 100 | 0.741 | Contact Map |
| 1f89A | 2 | 0.4805 | 100 | 0.742 | Contact Map |
| 2e11A | 4 | 0.4629 | 100 | 0.744 | Contact Map |
| 2dyuA | 2 | 0.498 | 100 | 0.746 | Contact Map |
| 4izuA | 1 | 0.4434 | 100 | 0.746 | Contact Map |
| 3ivzA | 2 | 0.4512 | 100 | 0.747 | Contact Map |
| 4f4hA | 2 | 0.5078 | 100 | 0.748 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)