May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org

GLP_1R

ID: 1488050730 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 463 (396)
Sequences: 424 (241.4)
Seq/Len: 1.071
Nf(neff/√len): 12.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.071).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
403_C406_N3.2731.00
403_C408_E3.1061.00
402_Y406_N2.5560.99
402_Y408_E2.3680.99
326_R329_C2.1650.98
404_F408_E2.0990.97
406_N409_V2.0440.97
75_G81_V2.0090.96
403_C407_N1.9750.96
67_D71_C1.9280.95
235_Y247_E1.9150.95
62_C71_C1.8820.94
403_C409_V1.8790.94
324_F349_L1.8710.94
151_G159_L1.8550.93
163_S181_L1.8520.93
64_R74_D1.8360.93
247_E274_W1.7460.90
352_S387_E1.7070.89
405_V408_E1.6730.88
395_G408_E1.6630.87
186_S240_N1.5850.83
233_M360_L1.5710.83
402_Y407_N1.5680.83
62_C67_D1.5620.82
120_W126_C1.5560.82
151_G155_S1.5190.80
402_Y405_V1.5100.79
62_C72_W1.5100.79
158_A396_L1.4900.78
247_E251_L1.4880.78
402_Y409_V1.4850.78
243_W247_E1.4730.77
395_G403_C1.4430.75
175_T178_Y1.4420.75
324_F352_S1.4300.74
244_L324_F1.4230.73
271_S319_V1.4140.73
227_R293_D1.4090.72
226_C247_E1.4090.72
404_F409_V1.4010.72
395_G406_N1.3980.71
122_D126_C1.3860.70
149_T198_D1.3850.70
349_L352_S1.3780.70
177_N190_R1.3760.70
287_V308_I1.3740.69
288_K294_E1.3680.69
177_N284_W1.3660.69
175_T179_I1.3640.69
196_I204_M1.3640.69
173_H177_N1.3630.69
398_V406_N1.3620.68
172_L235_Y1.3550.68
67_D86_P1.3540.68
190_R197_K1.3440.67
235_Y274_W1.3440.67
195_F352_S1.3400.67
352_S384_L1.3300.66
71_C86_P1.3250.65
247_E273_G1.3230.65
70_A83_V1.3220.65
244_L352_S1.3100.64
324_F412_E1.3070.64
152_Y392_S1.2970.63
162_A190_R1.2930.63
250_Y273_G1.2620.60
150_V366_I1.2570.59
335_L399_A1.2560.59
251_L274_W1.2520.59
110_W122_D1.2520.59
171_H324_F1.2510.59
176_R180_H1.2460.58
32_L35_T1.2340.57
235_Y243_W1.2310.57
336_K420_W1.2270.57
166_L280_F1.2220.56
62_C86_P1.2210.56
101_Y125_E1.2210.56
386_T389_S1.2180.56
398_V408_E1.2150.55
171_H190_R1.2090.55
171_H177_N1.2050.54
186_S363_H1.2020.54
108_G126_C1.1960.54
354_L358_P1.1900.53
300_N310_R1.1890.53
286_I290_L1.1880.53
389_S397_M1.1830.52
356_L361_G1.1830.52
321_F393_F1.1740.52
412_E417_W1.1650.51
187_F348_R1.1650.51
140_Q143_F1.1630.51
187_F198_D1.1480.49
230_F238_A1.1460.49
184_F191_A1.1450.49
234_Q239_A1.1430.49
252_Y326_R1.1420.49
191_A197_K1.1420.49
361_G394_Q1.1390.48
306_W310_R1.1390.48
244_L349_L1.1370.48
227_R353_T1.1370.48
241_Y288_K1.1360.48
197_K324_F1.1310.48
107_E282_V1.1300.47
108_G120_W1.1280.47
366_I386_T1.1260.47
166_L400_I1.1250.47
174_C178_Y1.1240.47
226_C235_Y1.1240.47
148_Y152_Y1.1220.47
197_K284_W1.1210.47
396_L400_I1.1180.46
104_C126_C1.1150.46
50_L64_R1.1150.46
266_F269_Y1.1150.46
242_Y272_I1.1130.46
395_G398_V1.1130.46
175_T186_S1.1120.46
120_W124_S1.1100.46
31_S35_T1.1080.45
187_F353_T1.1080.45
40_R366_I1.1060.45
349_L412_E1.1000.45
228_L411_L1.0990.45
235_Y251_L1.0980.44
154_L414_R1.0970.44
67_D72_W1.0920.44
327_V335_L1.0920.44
187_F240_N1.0900.44
258_S382_I1.0860.43
179_I246_V1.0850.43
275_G312_P1.0840.43
31_S34_E1.0770.42
149_T178_Y1.0760.42
163_S318_G1.0720.42
78_G92_A1.0720.42
321_F367_F1.0710.42
190_R284_W1.0690.42
246_V284_W1.0690.42
350_A357_I1.0680.42
240_N274_W1.0670.42
349_L388_L1.0650.41
148_Y179_I1.0630.41
240_N243_W1.0620.41
155_S159_L1.0620.41
177_N197_K1.0610.41
62_C73_P1.0600.41
178_Y198_D1.0590.41
167_L181_L1.0590.41
160_V417_W1.0580.41
85_C394_Q1.0580.41
246_V272_I1.0570.41
85_C110_W1.0530.40
247_E250_Y1.0510.40
66_F90_P1.0470.40
162_A177_N1.0460.40
152_Y174_C1.0460.40
415_K418_E1.0420.39
180_H183_L1.0390.39
69_Y383_K1.0360.39
352_S412_E1.0360.39
308_I396_L1.0350.39
72_W86_P1.0290.38
254_L288_K1.0290.38
190_R324_F1.0250.38
154_L282_V1.0250.38
66_F381_F1.0240.38
110_W218_L1.0230.38
235_Y254_L1.0220.37
328_I332_V1.0210.37
78_G195_F1.0210.37
245_L254_L1.0190.37
356_L394_Q1.0160.37
235_Y269_Y1.0150.37
298_T352_S1.0150.37
321_F384_L1.0150.37
143_F209_A1.0150.37
404_F407_N1.0150.37
174_C187_F1.0130.37
248_G251_L1.0120.37
226_C274_W1.0100.36
71_C85_C1.0090.36
179_I307_L1.0080.36
317_I352_S1.0080.36
150_V153_A1.0070.36
400_I416_S1.0060.36
162_A281_V1.0060.36
110_W123_L1.0050.36
86_P252_Y1.0050.36
152_Y162_A1.0040.36
198_D324_F1.0030.36
172_L327_V1.0030.36
245_L323_I1.0010.36
235_Y327_V1.0000.36
226_C251_L1.0000.36
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4k5yA30.54641000.635Contact Map
4l6rA10.82721000.69Contact Map
3c5tA10.22461000.836Contact Map
4jkvA20.81641000.841Contact Map
4ersA10.207399.90.849Contact Map
4qinA10.671799.90.851Contact Map
4rwfA1099.90.852Contact Map
2qkhA10.20399.90.857Contact Map
4hj0A20.198799.90.859Contact Map
3n94A10.272199.90.865Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.7995 seconds.