| GLP_1R |
| ID: | 1488050193 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 426 (387) | ||
| Sequences: | 676 (392.4) | ||
| Seq/Len: | 1.747 | ||
| Nf(neff/√len): | 19.9 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.747).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 366_C | 369_N | 3.531 | 1.00 |
| 366_C | 371_E | 3.053 | 1.00 |
| 289_R | 292_C | 2.698 | 1.00 |
| 365_Y | 371_E | 2.524 | 1.00 |
| 365_Y | 369_N | 2.402 | 1.00 |
| 41_G | 47_V | 2.366 | 1.00 |
| 33_D | 37_C | 2.279 | 1.00 |
| 367_F | 371_E | 2.215 | 0.99 |
| 369_N | 372_V | 2.212 | 0.99 |
| 28_C | 37_C | 2.180 | 0.99 |
| 126_T | 144_L | 2.175 | 0.99 |
| 30_R | 40_D | 2.092 | 0.99 |
| 28_C | 33_D | 2.070 | 0.99 |
| 28_C | 38_W | 2.009 | 0.99 |
| 114_G | 122_L | 1.971 | 0.98 |
| 366_C | 372_V | 1.894 | 0.98 |
| 36_A | 49_V | 1.814 | 0.97 |
| 33_D | 38_W | 1.800 | 0.97 |
| 315_S | 350_E | 1.778 | 0.96 |
| 198_Y | 210_E | 1.768 | 0.96 |
| 368_I | 371_E | 1.740 | 0.96 |
| 37_C | 52_P | 1.738 | 0.96 |
| 114_G | 128_I | 1.651 | 0.94 |
| 149_S | 203_N | 1.645 | 0.94 |
| 33_D | 52_P | 1.640 | 0.94 |
| 358_G | 366_C | 1.630 | 0.93 |
| 287_F | 312_L | 1.623 | 0.93 |
| 366_C | 370_N | 1.617 | 0.93 |
| 28_C | 52_P | 1.599 | 0.93 |
| 358_G | 369_N | 1.556 | 0.91 |
| 114_G | 118_S | 1.556 | 0.91 |
| 38_W | 51_C | 1.521 | 0.90 |
| 215_Y | 289_R | 1.488 | 0.88 |
| 37_C | 51_C | 1.488 | 0.88 |
| 121_A | 359_L | 1.484 | 0.88 |
| 361_V | 369_N | 1.484 | 0.88 |
| 270_L | 274_L | 1.476 | 0.88 |
| 198_Y | 206_W | 1.474 | 0.88 |
| 317_L | 321_P | 1.448 | 0.87 |
| 272_I | 276_I | 1.447 | 0.87 |
| 28_C | 51_C | 1.434 | 0.86 |
| 312_L | 315_S | 1.424 | 0.85 |
| 140_N | 153_R | 1.422 | 0.85 |
| 260_W | 275_P | 1.417 | 0.85 |
| 361_V | 371_E | 1.415 | 0.85 |
| 76_W | 92_C | 1.407 | 0.84 |
| 139_R | 143_H | 1.406 | 0.84 |
| 375_E | 380_W | 1.395 | 0.84 |
| 319_L | 324_G | 1.382 | 0.83 |
| 86_W | 92_C | 1.377 | 0.83 |
| 362_A | 366_C | 1.372 | 0.82 |
| 196_M | 323_L | 1.371 | 0.82 |
| 251_K | 257_E | 1.369 | 0.82 |
| 367_F | 372_V | 1.364 | 0.82 |
| 358_G | 371_E | 1.353 | 0.81 |
| 365_Y | 368_I | 1.350 | 0.81 |
| 378_K | 381_E | 1.348 | 0.81 |
| 73_E | 245_I | 1.346 | 0.81 |
| 115_Y | 125_A | 1.346 | 0.81 |
| 215_Y | 220_L | 1.344 | 0.80 |
| 134_H | 140_N | 1.321 | 0.79 |
| 138_T | 141_Y | 1.320 | 0.79 |
| 118_S | 122_L | 1.314 | 0.78 |
| 362_A | 371_E | 1.314 | 0.78 |
| 352_S | 360_M | 1.313 | 0.78 |
| 212_M | 286_I | 1.313 | 0.78 |
| 33_D | 51_C | 1.310 | 0.78 |
| 250_V | 271_I | 1.305 | 0.78 |
| 298_L | 362_A | 1.304 | 0.78 |
| 15_L | 30_R | 1.302 | 0.77 |
| 203_N | 326_H | 1.297 | 0.77 |
| 207_L | 287_F | 1.290 | 0.77 |
| 86_W | 90_S | 1.277 | 0.76 |
| 365_Y | 372_V | 1.272 | 0.75 |
| 215_Y | 292_C | 1.264 | 0.74 |
| 114_G | 147_F | 1.263 | 0.74 |
| 158_F | 315_S | 1.262 | 0.74 |
| 67_Y | 91_E | 1.261 | 0.74 |
| 207_L | 315_S | 1.254 | 0.74 |
| 212_M | 222_V | 1.253 | 0.74 |
| 114_G | 121_A | 1.249 | 0.73 |
| 134_H | 287_F | 1.240 | 0.72 |
| 206_W | 210_E | 1.239 | 0.72 |
| 71_T | 75_T | 1.221 | 0.71 |
| 140_N | 247_W | 1.221 | 0.71 |
| 114_G | 125_A | 1.220 | 0.71 |
| 291_I | 295_I | 1.220 | 0.71 |
| 203_N | 206_W | 1.219 | 0.71 |
| 144_L | 370_N | 1.215 | 0.70 |
| 210_E | 214_L | 1.209 | 0.70 |
| 129_L | 243_F | 1.208 | 0.70 |
| 137_C | 150_F | 1.205 | 0.69 |
| 359_L | 363_I | 1.205 | 0.69 |
| 211_G | 214_L | 1.204 | 0.69 |
| 38_W | 52_P | 1.203 | 0.69 |
| 47_V | 221_S | 1.201 | 0.69 |
| 211_G | 232_Y | 1.195 | 0.68 |
| 358_G | 361_V | 1.192 | 0.68 |
| 363_I | 378_K | 1.191 | 0.68 |
| 213_Y | 236_G | 1.190 | 0.68 |
| 138_T | 149_S | 1.186 | 0.68 |
| 321_P | 357_Q | 1.184 | 0.67 |
| 205_Y | 235_I | 1.174 | 0.66 |
| 227_R | 328_V | 1.174 | 0.66 |
| 192_V | 224_S | 1.172 | 0.66 |
| 269_W | 273_R | 1.165 | 0.66 |
| 70_C | 76_W | 1.161 | 0.65 |
| 232_Y | 289_R | 1.160 | 0.65 |
| 28_C | 32_F | 1.160 | 0.65 |
| 147_F | 154_A | 1.159 | 0.65 |
| 190_R | 316_T | 1.158 | 0.65 |
| 129_L | 363_I | 1.153 | 0.64 |
| 283_N | 297_K | 1.152 | 0.64 |
| 32_F | 56_P | 1.152 | 0.64 |
| 375_E | 378_K | 1.147 | 0.64 |
| 279_A | 288_I | 1.147 | 0.64 |
| 365_Y | 370_N | 1.147 | 0.64 |
| 364_L | 367_F | 1.145 | 0.64 |
| 136_H | 140_N | 1.145 | 0.64 |
| 249_T | 253_L | 1.138 | 0.63 |
| 88_N | 92_C | 1.138 | 0.63 |
| 206_W | 237_W | 1.136 | 0.63 |
| 357_Q | 369_N | 1.134 | 0.62 |
| 313_A | 320_I | 1.133 | 0.62 |
| 284_F | 292_C | 1.132 | 0.62 |
| 111_Y | 115_Y | 1.131 | 0.62 |
| 198_Y | 237_W | 1.129 | 0.62 |
| 319_L | 357_Q | 1.129 | 0.62 |
| 150_F | 153_R | 1.127 | 0.62 |
| 154_A | 160_K | 1.122 | 0.61 |
| 11_C | 15_L | 1.120 | 0.61 |
| 159_I | 167_M | 1.120 | 0.61 |
| 189_C | 210_E | 1.119 | 0.61 |
| 151_I | 362_A | 1.118 | 0.61 |
| 28_C | 39_P | 1.116 | 0.61 |
| 197_Q | 202_A | 1.113 | 0.60 |
| 9_L | 14_Y | 1.113 | 0.60 |
| 210_E | 237_W | 1.112 | 0.60 |
| 209_V | 235_I | 1.111 | 0.60 |
| 229_F | 232_Y | 1.110 | 0.60 |
| 145_N | 225_E | 1.109 | 0.60 |
| 74_G | 77_L | 1.107 | 0.60 |
| 287_F | 315_S | 1.105 | 0.60 |
| 139_R | 147_F | 1.103 | 0.59 |
| 140_N | 154_A | 1.102 | 0.59 |
| 143_H | 146_L | 1.101 | 0.59 |
| 41_G | 49_V | 1.101 | 0.59 |
| 135_L | 198_Y | 1.099 | 0.59 |
| 207_L | 218_L | 1.098 | 0.59 |
| 32_F | 35_Y | 1.098 | 0.59 |
| 209_V | 247_W | 1.096 | 0.59 |
| 203_N | 279_A | 1.092 | 0.58 |
| 312_L | 350_E | 1.091 | 0.58 |
| 152_L | 199_C | 1.091 | 0.58 |
| 74_G | 86_W | 1.091 | 0.58 |
| 329_I | 349_T | 1.090 | 0.58 |
| 111_Y | 142_I | 1.090 | 0.58 |
| 112_T | 161_D | 1.086 | 0.58 |
| 31_T | 40_D | 1.085 | 0.57 |
| 363_I | 368_I | 1.084 | 0.57 |
| 362_A | 369_N | 1.083 | 0.57 |
| 138_T | 142_I | 1.081 | 0.57 |
| 211_G | 215_Y | 1.078 | 0.57 |
| 141_Y | 161_D | 1.076 | 0.56 |
| 114_G | 119_F | 1.076 | 0.56 |
| 358_G | 362_A | 1.069 | 0.56 |
| 70_C | 92_C | 1.067 | 0.56 |
| 240_P | 247_W | 1.064 | 0.55 |
| 207_L | 312_L | 1.062 | 0.55 |
| 32_F | 217_L | 1.057 | 0.54 |
| 118_S | 132_F | 1.054 | 0.54 |
| 155_I | 159_I | 1.053 | 0.54 |
| 135_L | 143_H | 1.051 | 0.54 |
| 249_T | 252_Y | 1.045 | 0.53 |
| 128_I | 135_L | 1.043 | 0.53 |
| 124_I | 148_T | 1.042 | 0.53 |
| 190_R | 256_D | 1.041 | 0.53 |
| 155_I | 377_R | 1.040 | 0.53 |
| 325_T | 333_I | 1.040 | 0.53 |
| 198_Y | 203_N | 1.036 | 0.52 |
| 44_G | 51_C | 1.033 | 0.52 |
| 194_V | 248_G | 1.027 | 0.51 |
| 32_F | 344_F | 1.024 | 0.51 |
| 198_Y | 214_L | 1.023 | 0.51 |
| 203_N | 237_W | 1.023 | 0.51 |
| 323_L | 351_L | 1.023 | 0.51 |
| 229_F | 364_L | 1.020 | 0.50 |
| 259_C | 275_P | 1.020 | 0.50 |
| 140_N | 160_K | 1.017 | 0.50 |
| 327_E | 355_S | 1.017 | 0.50 |
| 364_L | 369_N | 1.012 | 0.50 |
| 125_A | 140_N | 1.005 | 0.49 |
| 150_F | 203_N | 1.003 | 0.49 |
| 13_K | 16_Y | 1.002 | 0.49 |
| 240_P | 243_F | 1.000 | 0.48 |
| 290_V | 294_I | 1.000 | 0.48 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4k5yA | 3 | 0.5939 | 100 | 0.584 | Contact Map |
| 4l6rA | 1 | 0.8592 | 100 | 0.642 | Contact Map |
| 4jkvA | 2 | 0.8052 | 100 | 0.811 | Contact Map |
| 4rwfA | 1 | 0 | 99.9 | 0.818 | Contact Map |
| 3c5tA | 1 | 0.2254 | 99.9 | 0.819 | Contact Map |
| 4ersA | 1 | 0.2136 | 99.9 | 0.825 | Contact Map |
| 4hj0A | 2 | 0.2042 | 99.9 | 0.829 | Contact Map |
| 4qinA | 1 | 0.7207 | 99.9 | 0.829 | Contact Map |
| 2qkhA | 1 | 0.2042 | 99.9 | 0.832 | Contact Map |
| 3n94A | 1 | 0.216 | 99.9 | 0.838 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)