| FUT5 |
| ID: | 1486891776 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 374 (305) | ||
| Sequences: | 305 (231.1) | ||
| Seq/Len: | 1.000 | ||
| Nf(neff/√len): | 13.2 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.000).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 233_D | 254_Y | 2.942 | 1.00 |
| 273_L | 298_L | 2.642 | 1.00 |
| 222_Y | 312_P | 2.503 | 0.99 |
| 255_K | 323_D | 2.398 | 0.99 |
| 225_S | 228_A | 2.340 | 0.99 |
| 289_P | 305_H | 2.187 | 0.98 |
| 215_P | 220_V | 2.150 | 0.97 |
| 151_E | 262_N | 2.009 | 0.96 |
| 222_Y | 260_F | 1.976 | 0.95 |
| 145_W | 165_Y | 1.940 | 0.94 |
| 151_E | 272_K | 1.934 | 0.94 |
| 151_E | 271_E | 1.813 | 0.91 |
| 262_N | 268_Y | 1.806 | 0.91 |
| 262_N | 272_K | 1.796 | 0.91 |
| 304_I | 332_Y | 1.747 | 0.89 |
| 268_Y | 271_E | 1.724 | 0.88 |
| 103_D | 358_Q | 1.717 | 0.88 |
| 262_N | 271_E | 1.710 | 0.88 |
| 85_F | 122_H | 1.694 | 0.87 |
| 81_W | 135_P | 1.678 | 0.86 |
| 288_G | 305_H | 1.675 | 0.86 |
| 238_S | 265_H | 1.671 | 0.86 |
| 213_W | 238_S | 1.665 | 0.86 |
| 211_S | 262_N | 1.655 | 0.85 |
| 288_G | 315_L | 1.648 | 0.85 |
| 207_A | 254_Y | 1.629 | 0.84 |
| 288_G | 293_N | 1.623 | 0.84 |
| 124_W | 155_N | 1.581 | 0.82 |
| 321_E | 328_R | 1.572 | 0.81 |
| 328_R | 331_S | 1.572 | 0.81 |
| 229_H | 316_A | 1.570 | 0.81 |
| 318_Y | 322_L | 1.564 | 0.81 |
| 295_E | 303_F | 1.542 | 0.79 |
| 139_R | 169_T | 1.511 | 0.77 |
| 230_L | 316_A | 1.483 | 0.76 |
| 118_A | 357_L | 1.483 | 0.76 |
| 124_W | 152_S | 1.471 | 0.75 |
| 111_S | 285_V | 1.468 | 0.75 |
| 287_L | 306_V | 1.430 | 0.72 |
| 109_D | 114_P | 1.429 | 0.72 |
| 259_A | 309_F | 1.426 | 0.71 |
| 116_A | 303_F | 1.418 | 0.71 |
| 118_A | 146_I | 1.417 | 0.71 |
| 151_E | 211_S | 1.400 | 0.69 |
| 152_S | 212_N | 1.398 | 0.69 |
| 289_P | 307_D | 1.379 | 0.68 |
| 202_K | 270_T | 1.366 | 0.67 |
| 206_V | 285_V | 1.365 | 0.67 |
| 259_A | 277_A | 1.358 | 0.66 |
| 176_S | 325_D | 1.356 | 0.66 |
| 183_G | 259_A | 1.355 | 0.66 |
| 152_S | 155_N | 1.353 | 0.66 |
| 205_L | 323_D | 1.347 | 0.65 |
| 298_L | 301_D | 1.342 | 0.65 |
| 322_L | 332_Y | 1.328 | 0.64 |
| 302_A | 329_Y | 1.325 | 0.63 |
| 213_W | 220_V | 1.316 | 0.62 |
| 233_D | 239_H | 1.316 | 0.62 |
| 321_E | 325_D | 1.314 | 0.62 |
| 168_L | 366_V | 1.312 | 0.62 |
| 168_L | 356_K | 1.303 | 0.61 |
| 309_F | 318_Y | 1.302 | 0.61 |
| 213_W | 265_H | 1.299 | 0.61 |
| 147_W | 220_V | 1.290 | 0.60 |
| 313_K | 317_R | 1.289 | 0.60 |
| 152_S | 263_S | 1.286 | 0.60 |
| 151_E | 268_Y | 1.286 | 0.60 |
| 268_Y | 272_K | 1.279 | 0.59 |
| 151_E | 212_N | 1.271 | 0.58 |
| 217_S | 264_L | 1.270 | 0.58 |
| 302_A | 335_W | 1.231 | 0.55 |
| 144_R | 168_L | 1.231 | 0.55 |
| 202_K | 329_Y | 1.228 | 0.55 |
| 110_S | 173_R | 1.223 | 0.54 |
| 176_S | 254_Y | 1.223 | 0.54 |
| 235_Y | 331_S | 1.221 | 0.54 |
| 213_W | 353_A | 1.214 | 0.53 |
| 258_L | 315_L | 1.213 | 0.53 |
| 264_L | 289_P | 1.209 | 0.53 |
| 179_F | 185_L | 1.204 | 0.52 |
| 153_P | 172_Y | 1.199 | 0.52 |
| 286_V | 304_I | 1.199 | 0.52 |
| 293_N | 305_H | 1.198 | 0.52 |
| 269_I | 295_E | 1.197 | 0.52 |
| 76_L | 357_L | 1.196 | 0.52 |
| 175_D | 208_W | 1.193 | 0.51 |
| 215_P | 218_A | 1.192 | 0.51 |
| 286_V | 294_Y | 1.183 | 0.50 |
| 230_L | 319_L | 1.181 | 0.50 |
| 146_I | 353_A | 1.178 | 0.50 |
| 255_K | 278_L | 1.174 | 0.50 |
| 219_R | 268_Y | 1.171 | 0.49 |
| 182_Y | 270_T | 1.170 | 0.49 |
| 176_S | 207_A | 1.162 | 0.48 |
| 289_P | 292_S | 1.160 | 0.48 |
| 174_S | 296_R | 1.157 | 0.48 |
| 288_G | 292_S | 1.153 | 0.48 |
| 139_R | 167_N | 1.151 | 0.47 |
| 288_G | 294_Y | 1.151 | 0.47 |
| 153_P | 270_T | 1.144 | 0.47 |
| 145_W | 258_L | 1.140 | 0.46 |
| 167_N | 366_V | 1.138 | 0.46 |
| 169_T | 208_W | 1.129 | 0.45 |
| 145_W | 349_A | 1.123 | 0.45 |
| 178_I | 278_L | 1.123 | 0.45 |
| 291_R | 295_E | 1.117 | 0.44 |
| 145_W | 180_T | 1.116 | 0.44 |
| 209_A | 217_S | 1.116 | 0.44 |
| 325_D | 328_R | 1.111 | 0.44 |
| 256_F | 299_P | 1.111 | 0.44 |
| 212_N | 262_N | 1.103 | 0.43 |
| 325_D | 332_Y | 1.102 | 0.43 |
| 274_W | 369_I | 1.101 | 0.43 |
| 211_S | 272_K | 1.100 | 0.43 |
| 256_F | 284_P | 1.087 | 0.42 |
| 257_Y | 271_E | 1.083 | 0.41 |
| 302_A | 333_F | 1.082 | 0.41 |
| 318_Y | 321_E | 1.074 | 0.40 |
| 305_H | 353_A | 1.072 | 0.40 |
| 305_H | 308_D | 1.069 | 0.40 |
| 182_Y | 263_S | 1.063 | 0.39 |
| 207_A | 233_D | 1.063 | 0.39 |
| 207_A | 323_D | 1.059 | 0.39 |
| 250_T | 254_Y | 1.057 | 0.39 |
| 219_R | 262_N | 1.056 | 0.39 |
| 287_L | 303_F | 1.055 | 0.39 |
| 219_R | 272_K | 1.054 | 0.39 |
| 122_H | 211_S | 1.052 | 0.38 |
| 182_Y | 211_S | 1.049 | 0.38 |
| 226_L | 316_A | 1.049 | 0.38 |
| 121_V | 202_K | 1.047 | 0.38 |
| 117_D | 167_N | 1.035 | 0.37 |
| 79_L | 104_C | 1.034 | 0.37 |
| 322_L | 331_S | 1.032 | 0.37 |
| 207_A | 239_H | 1.030 | 0.37 |
| 212_N | 272_K | 1.029 | 0.36 |
| 238_S | 373_F | 1.028 | 0.36 |
| 224_Q | 227_Q | 1.027 | 0.36 |
| 129_N | 283_V | 1.024 | 0.36 |
| 310_Q | 320_Q | 1.023 | 0.36 |
| 318_Y | 367_R | 1.012 | 0.35 |
| 94_C | 101_A | 1.011 | 0.35 |
| 289_P | 293_N | 1.011 | 0.35 |
| 226_L | 258_L | 1.011 | 0.35 |
| 334_R | 337_E | 1.011 | 0.35 |
| 121_V | 178_I | 1.009 | 0.35 |
| 293_N | 373_F | 1.004 | 0.34 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2nzwA | 3 | 0.7647 | 100 | 0.547 | Contact Map |
| 3qhpA | 2 | 0.4171 | 57.6 | 0.972 | Contact Map |
| 2f9fA | 1 | 0.4332 | 47.2 | 0.974 | Contact Map |
| 3h16A | 4 | 0.3155 | 26.1 | 0.977 | Contact Map |
| 4lzpB | 4 | 0.3957 | 12.8 | 0.98 | Contact Map |
| 3j7aY | 1 | 0.1684 | 9.9 | 0.981 | Contact Map |
| 3k4hA | 2 | 0.4305 | 9.3 | 0.981 | Contact Map |
| 3ab3B | 2 | 0.1524 | 9.1 | 0.982 | Contact Map |
| 2qu7A | 2 | 0.4091 | 8.7 | 0.982 | Contact Map |
| 3c3kA | 2 | 0.4171 | 7.7 | 0.982 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)