| glucose transporter |
| ID: | 1486400559 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 530 (460) | ||
| Sequences: | 8519 (4584.6) | ||
| Seq/Len: | 18.520 | ||
| Nf(neff/√len): | 213.8 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 18.520).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 433_A | 464_V | 5.318 | 1.00 |
| 250_S | 253_E | 4.007 | 1.00 |
| 362_G | 365_K | 3.858 | 1.00 |
| 422_T | 465_I | 3.857 | 1.00 |
| 433_A | 460_F | 3.695 | 1.00 |
| 145_L | 378_L | 3.412 | 1.00 |
| 468_T | 472_T | 3.179 | 1.00 |
| 176_Y | 180_S | 3.177 | 1.00 |
| 426_G | 469_S | 3.087 | 1.00 |
| 415_L | 465_I | 2.902 | 1.00 |
| 423_T | 472_T | 2.889 | 1.00 |
| 353_Y | 361_S | 2.829 | 1.00 |
| 178_A | 278_A | 2.810 | 1.00 |
| 358_S | 365_K | 2.789 | 1.00 |
| 466_A | 470_L | 2.736 | 1.00 |
| 429_I | 468_T | 2.666 | 1.00 |
| 368_N | 372_T | 2.621 | 1.00 |
| 364_H | 368_N | 2.595 | 1.00 |
| 141_A | 382_C | 2.573 | 1.00 |
| 423_T | 426_G | 2.561 | 1.00 |
| 437_F | 460_F | 2.549 | 1.00 |
| 464_V | 468_T | 2.531 | 1.00 |
| 93_F | 422_T | 2.488 | 1.00 |
| 153_L | 247_A | 2.462 | 1.00 |
| 14_X | 19_G | 2.389 | 1.00 |
| 372_T | 376_V | 2.384 | 1.00 |
| 419_W | 422_T | 2.368 | 1.00 |
| 425_K | 472_T | 2.363 | 1.00 |
| 22_L | 157_V | 2.333 | 1.00 |
| 430_G | 465_I | 2.326 | 1.00 |
| 13_X | 20_K | 2.299 | 1.00 |
| 17_X | 20_K | 2.207 | 1.00 |
| 90_E | 98_K | 2.201 | 1.00 |
| 429_I | 464_V | 2.176 | 1.00 |
| 412_V | 427_A | 2.115 | 1.00 |
| 138_L | 142_L | 2.114 | 1.00 |
| 348_F | 373_A | 2.101 | 1.00 |
| 353_Y | 369_V | 2.088 | 1.00 |
| 46_G | 307_D | 2.080 | 1.00 |
| 249_K | 253_E | 2.066 | 1.00 |
| 252_S | 256_K | 2.033 | 1.00 |
| 415_L | 462_M | 2.028 | 1.00 |
| 350_M | 354_K | 2.019 | 1.00 |
| 88_F | 416_G | 2.010 | 1.00 |
| 426_G | 430_G | 1.998 | 1.00 |
| 323_G | 327_A | 1.996 | 1.00 |
| 253_E | 256_K | 1.994 | 1.00 |
| 354_K | 366_L | 1.989 | 1.00 |
| 426_G | 468_T | 1.946 | 1.00 |
| 419_W | 470_L | 1.931 | 1.00 |
| 132_P | 135_Y | 1.900 | 1.00 |
| 425_K | 429_I | 1.894 | 1.00 |
| 29_A | 161_V | 1.885 | 1.00 |
| 79_E | 251_V | 1.882 | 1.00 |
| 279_A | 309_A | 1.882 | 1.00 |
| 421_K | 470_L | 1.875 | 1.00 |
| 26_A | 161_V | 1.862 | 1.00 |
| 170_G | 328_A | 1.828 | 1.00 |
| 353_Y | 365_K | 1.821 | 1.00 |
| 38_A | 120_L | 1.818 | 1.00 |
| 348_F | 352_I | 1.803 | 1.00 |
| 172_F | 176_Y | 1.801 | 1.00 |
| 463_V | 467_F | 1.787 | 1.00 |
| 368_N | 371_R | 1.775 | 1.00 |
| 144_A | 337_L | 1.756 | 1.00 |
| 419_W | 466_A | 1.740 | 1.00 |
| 361_S | 365_K | 1.740 | 1.00 |
| 357_I | 361_S | 1.693 | 1.00 |
| 151_G | 155_A | 1.675 | 1.00 |
| 421_K | 473_S | 1.660 | 1.00 |
| 341_L | 378_L | 1.649 | 1.00 |
| 365_K | 369_V | 1.640 | 1.00 |
| 252_S | 255_Q | 1.630 | 1.00 |
| 27_V | 248_A | 1.627 | 1.00 |
| 87_E | 351_D | 1.626 | 1.00 |
| 24_W | 253_E | 1.621 | 1.00 |
| 379_I | 383_L | 1.616 | 1.00 |
| 82_I | 91_K | 1.608 | 1.00 |
| 423_T | 469_S | 1.599 | 1.00 |
| 344_T | 377_A | 1.593 | 1.00 |
| 94_N | 424_S | 1.592 | 1.00 |
| 353_Y | 358_S | 1.588 | 1.00 |
| 84_T | 346_T | 1.585 | 1.00 |
| 459_L | 462_M | 1.571 | 1.00 |
| 436_P | 440_F | 1.570 | 1.00 |
| 142_L | 146_V | 1.543 | 1.00 |
| 141_A | 378_L | 1.539 | 1.00 |
| 358_S | 361_S | 1.534 | 1.00 |
| 348_F | 377_A | 1.531 | 1.00 |
| 159_T | 338_A | 1.522 | 1.00 |
| 93_F | 465_I | 1.516 | 1.00 |
| 194_K | 197_D | 1.513 | 1.00 |
| 30_S | 339_S | 1.512 | 1.00 |
| 459_L | 463_V | 1.501 | 1.00 |
| 92_R | 416_G | 1.500 | 1.00 |
| 134_M | 386_P | 1.497 | 1.00 |
| 433_A | 437_F | 1.495 | 1.00 |
| 16_X | 370_G | 1.493 | 1.00 |
| 18_A | 153_L | 1.483 | 1.00 |
| 361_S | 366_L | 1.482 | 1.00 |
| 93_F | 416_G | 1.479 | 1.00 |
| 122_G | 137_I | 1.476 | 1.00 |
| 88_F | 92_R | 1.471 | 1.00 |
| 349_T | 370_G | 1.471 | 1.00 |
| 135_Y | 138_L | 1.468 | 1.00 |
| 121_G | 140_L | 1.467 | 1.00 |
| 34_A | 339_S | 1.467 | 1.00 |
| 90_E | 355_E | 1.444 | 1.00 |
| 95_K | 424_S | 1.430 | 1.00 |
| 87_E | 350_M | 1.427 | 1.00 |
| 469_S | 472_T | 1.419 | 1.00 |
| 28_G | 260_F | 1.414 | 1.00 |
| 429_I | 433_A | 1.391 | 0.99 |
| 353_Y | 357_I | 1.386 | 0.99 |
| 138_L | 382_C | 1.360 | 0.99 |
| 11_X | 14_X | 1.353 | 0.99 |
| 160_D | 338_A | 1.343 | 0.99 |
| 16_X | 346_T | 1.337 | 0.99 |
| 365_K | 368_N | 1.331 | 0.99 |
| 348_F | 353_Y | 1.329 | 0.99 |
| 163_Q | 338_A | 1.329 | 0.99 |
| 48_G | 277_I | 1.327 | 0.99 |
| 412_V | 431_V | 1.322 | 0.99 |
| 92_R | 419_W | 1.320 | 0.99 |
| 18_A | 22_L | 1.319 | 0.99 |
| 160_D | 244_R | 1.317 | 0.99 |
| 422_T | 426_G | 1.315 | 0.99 |
| 24_W | 260_F | 1.312 | 0.99 |
| 30_S | 244_R | 1.308 | 0.99 |
| 138_L | 386_P | 1.306 | 0.99 |
| 56_A | 60_W | 1.293 | 0.99 |
| 165_F | 169_L | 1.284 | 0.99 |
| 175_T | 271_L | 1.280 | 0.99 |
| 173_M | 177_M | 1.277 | 0.99 |
| 32_I | 264_L | 1.276 | 0.99 |
| 24_W | 28_G | 1.275 | 0.99 |
| 367_V | 371_R | 1.266 | 0.99 |
| 30_S | 33_A | 1.260 | 0.99 |
| 73_F | 414_L | 1.254 | 0.99 |
| 466_A | 469_S | 1.253 | 0.99 |
| 314_T | 325_V | 1.248 | 0.99 |
| 137_I | 382_C | 1.235 | 0.98 |
| 187_G | 190_A | 1.233 | 0.98 |
| 430_G | 461_T | 1.233 | 0.98 |
| 349_T | 369_V | 1.232 | 0.98 |
| 147_Y | 163_Q | 1.223 | 0.98 |
| 90_E | 95_K | 1.222 | 0.98 |
| 190_A | 193_S | 1.222 | 0.98 |
| 373_A | 376_V | 1.210 | 0.98 |
| 239_Q | 243_Q | 1.210 | 0.98 |
| 419_W | 426_G | 1.206 | 0.98 |
| 173_M | 321_V | 1.202 | 0.98 |
| 26_A | 157_V | 1.191 | 0.98 |
| 411_A | 462_M | 1.191 | 0.98 |
| 131_I | 136_S | 1.183 | 0.98 |
| 126_E | 133_L | 1.178 | 0.98 |
| 48_G | 55_I | 1.172 | 0.98 |
| 460_F | 464_V | 1.169 | 0.97 |
| 471_S | 474_I | 1.168 | 0.97 |
| 363_D | 366_L | 1.165 | 0.97 |
| 147_Y | 338_A | 1.162 | 0.97 |
| 464_V | 467_F | 1.156 | 0.97 |
| 424_S | 428_I | 1.154 | 0.97 |
| 134_M | 138_L | 1.149 | 0.97 |
| 103_V | 352_I | 1.146 | 0.97 |
| 419_W | 469_S | 1.142 | 0.97 |
| 462_M | 466_A | 1.139 | 0.97 |
| 98_K | 351_D | 1.139 | 0.97 |
| 408_G | 461_T | 1.137 | 0.97 |
| 33_A | 244_R | 1.135 | 0.97 |
| 239_Q | 343_S | 1.133 | 0.97 |
| 419_W | 423_T | 1.131 | 0.97 |
| 148_S | 338_A | 1.130 | 0.97 |
| 99_T | 352_I | 1.129 | 0.97 |
| 97_L | 428_I | 1.129 | 0.97 |
| 423_T | 468_T | 1.129 | 0.97 |
| 118_L | 137_I | 1.123 | 0.97 |
| 430_G | 434_S | 1.122 | 0.97 |
| 15_X | 374_A | 1.122 | 0.97 |
| 123_L | 305_N | 1.122 | 0.97 |
| 30_S | 34_A | 1.115 | 0.96 |
| 147_Y | 334_V | 1.110 | 0.96 |
| 415_L | 422_T | 1.107 | 0.96 |
| 372_T | 375_V | 1.100 | 0.96 |
| 194_K | 198_A | 1.090 | 0.96 |
| 135_Y | 139_G | 1.086 | 0.96 |
| 177_M | 181_F | 1.084 | 0.96 |
| 314_T | 317_L | 1.084 | 0.96 |
| 345_A | 349_T | 1.080 | 0.95 |
| 353_Y | 359_P | 1.078 | 0.95 |
| 156_V | 159_T | 1.075 | 0.95 |
| 149_I | 375_V | 1.069 | 0.95 |
| 118_L | 141_A | 1.069 | 0.95 |
| 100_I | 428_I | 1.057 | 0.95 |
| 248_A | 254_A | 1.057 | 0.95 |
| 259_V | 263_F | 1.053 | 0.95 |
| 369_V | 373_A | 1.048 | 0.94 |
| 312_W | 315_Q | 1.047 | 0.94 |
| 10_X | 14_X | 1.046 | 0.94 |
| 383_L | 387_M | 1.043 | 0.94 |
| 144_A | 338_A | 1.041 | 0.94 |
| 361_S | 369_V | 1.041 | 0.94 |
| 426_G | 429_I | 1.038 | 0.94 |
| 23_P | 253_E | 1.036 | 0.94 |
| 181_F | 282_I | 1.033 | 0.94 |
| 144_A | 334_V | 1.028 | 0.94 |
| 187_G | 191_G | 1.028 | 0.94 |
| 193_S | 196_V | 1.024 | 0.93 |
| 159_T | 163_Q | 1.022 | 0.93 |
| 37_S | 113_N | 1.021 | 0.93 |
| 416_G | 427_A | 1.015 | 0.93 |
| 320_G | 324_V | 1.013 | 0.93 |
| 29_A | 164_V | 1.013 | 0.93 |
| 80_K | 91_K | 1.011 | 0.93 |
| 428_I | 432_V | 1.010 | 0.93 |
| 376_V | 380_I | 1.001 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3dh4A | 4 | 0.9321 | 100 | 0.161 | Contact Map |
| 2xq2A | 1 | 0.9547 | 100 | 0.171 | Contact Map |
| 3l1lA | 1 | 0.7698 | 98.9 | 0.886 | Contact Map |
| 4wgvA | 2 | 0.7038 | 98.7 | 0.894 | Contact Map |
| 2jlnA | 1 | 0.783 | 98.6 | 0.896 | Contact Map |
| 3giaA | 1 | 0.7792 | 98.4 | 0.902 | Contact Map |
| 4djkA | 2 | 0.783 | 97.9 | 0.913 | Contact Map |
| 2wswA | 1 | 0.7377 | 96.1 | 0.935 | Contact Map |
| 4c7rA | 3 | 0.8 | 95 | 0.939 | Contact Map |
| 2a65A | 1 | 0.8453 | 60.9 | 0.961 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)