| 1aoz0 |
| ID: | 1479987395 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 542 (535) | ||
| Sequences: | 6059 (4129.4) | ||
| Seq/Len: | 11.325 | ||
| Nf(neff/√len): | 178.5 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 11.325).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 37_R | 122_D | 4.348 | 1.00 |
| 45_V | 92_N | 4.169 | 1.00 |
| 43_S | 92_N | 3.738 | 1.00 |
| 214_T | 266_L | 3.574 | 1.00 |
| 7_K | 47_E | 3.498 | 1.00 |
| 9_E | 51_K | 3.443 | 1.00 |
| 15_W | 24_V | 3.341 | 1.00 |
| 35_T | 118_S | 3.299 | 1.00 |
| 47_E | 90_F | 3.171 | 1.00 |
| 210_S | 213_K | 3.118 | 1.00 |
| 7_K | 49_T | 3.027 | 1.00 |
| 241_E | 264_S | 3.015 | 1.00 |
| 219_I | 265_V | 3.000 | 1.00 |
| 484_V | 492_T | 2.969 | 1.00 |
| 261_E | 481_R | 2.840 | 1.00 |
| 219_I | 230_F | 2.801 | 1.00 |
| 13_M | 26_G | 2.793 | 1.00 |
| 49_T | 88_T | 2.780 | 1.00 |
| 48_L | 83_I | 2.772 | 1.00 |
| 214_T | 268_T | 2.669 | 1.00 |
| 217_I | 265_V | 2.658 | 1.00 |
| 453_H | 524_E | 2.650 | 1.00 |
| 236_Q | 253_S | 2.621 | 1.00 |
| 237_L | 265_V | 2.619 | 1.00 |
| 232_I | 267_I | 2.590 | 1.00 |
| 212_K | 269_T | 2.537 | 1.00 |
| 236_Q | 251_Y | 2.478 | 1.00 |
| 14_F | 21_E | 2.455 | 1.00 |
| 5_H | 47_E | 2.384 | 1.00 |
| 5_H | 45_V | 2.361 | 1.00 |
| 423_K | 426_E | 2.343 | 1.00 |
| 219_I | 255_I | 2.334 | 1.00 |
| 245_N | 454_D | 2.249 | 1.00 |
| 216_R | 240_V | 2.244 | 1.00 |
| 27_I | 103_Y | 2.209 | 1.00 |
| 279_V | 297_L | 2.176 | 1.00 |
| 206_I | 298_N | 2.170 | 1.00 |
| 141_L | 222_T | 2.135 | 1.00 |
| 72_A | 89_F | 2.051 | 1.00 |
| 257_I | 263_Y | 2.022 | 1.00 |
| 118_S | 220_A | 1.974 | 1.00 |
| 84_N | 87_E | 1.967 | 1.00 |
| 18_N | 147_H | 1.967 | 1.00 |
| 216_R | 264_S | 1.935 | 1.00 |
| 101_F | 244_G | 1.932 | 1.00 |
| 26_G | 31_F | 1.899 | 1.00 |
| 432_L | 448_W | 1.889 | 1.00 |
| 11_E | 51_K | 1.887 | 1.00 |
| 14_F | 23_I | 1.885 | 1.00 |
| 9_E | 49_T | 1.823 | 1.00 |
| 431_I | 493_A | 1.821 | 1.00 |
| 38_A | 44_V | 1.815 | 1.00 |
| 210_S | 300_L | 1.810 | 1.00 |
| 6_Y | 38_A | 1.809 | 1.00 |
| 66_Q | 69_T | 1.771 | 1.00 |
| 255_I | 265_V | 1.758 | 1.00 |
| 44_V | 119_L | 1.752 | 1.00 |
| 11_E | 26_G | 1.746 | 1.00 |
| 209_V | 269_T | 1.743 | 1.00 |
| 258_Y | 485_V | 1.734 | 1.00 |
| 39_N | 122_D | 1.727 | 1.00 |
| 429_D | 458_L | 1.699 | 1.00 |
| 525_G | 528_K | 1.681 | 1.00 |
| 43_S | 94_T | 1.664 | 1.00 |
| 455_F | 494_I | 1.658 | 1.00 |
| 427_V | 497_V | 1.654 | 1.00 |
| 233_G | 280_S | 1.652 | 1.00 |
| 116_Y | 143_S | 1.651 | 1.00 |
| 432_L | 506_F | 1.650 | 1.00 |
| 56_G | 84_N | 1.643 | 1.00 |
| 508_C | 513_H | 1.625 | 1.00 |
| 40_A | 123_P | 1.608 | 1.00 |
| 238_L | 251_Y | 1.603 | 1.00 |
| 430_V | 494_I | 1.595 | 1.00 |
| 223_T | 228_L | 1.594 | 1.00 |
| 509_H | 513_H | 1.588 | 1.00 |
| 176_R | 205_Y | 1.587 | 1.00 |
| 503_V | 523_A | 1.576 | 1.00 |
| 487_F | 490_G | 1.571 | 1.00 |
| 459_G | 491_W | 1.554 | 1.00 |
| 429_D | 495_R | 1.553 | 1.00 |
| 10_V | 57_V | 1.538 | 1.00 |
| 262_S | 482_N | 1.535 | 1.00 |
| 136_G | 213_K | 1.530 | 1.00 |
| 149_S | 152_K | 1.524 | 1.00 |
| 248_Q | 477_N | 1.522 | 1.00 |
| 432_L | 494_I | 1.513 | 1.00 |
| 242_A | 263_Y | 1.494 | 1.00 |
| 344_F | 347_R | 1.492 | 1.00 |
| 47_E | 88_T | 1.489 | 1.00 |
| 112_S | 224_A | 1.487 | 1.00 |
| 140_L | 219_I | 1.486 | 1.00 |
| 232_I | 279_V | 1.483 | 1.00 |
| 346_R | 350_L | 1.477 | 1.00 |
| 230_F | 279_V | 1.471 | 1.00 |
| 323_R | 326_N | 1.468 | 1.00 |
| 103_Y | 117_G | 1.464 | 1.00 |
| 324_S | 327_F | 1.463 | 1.00 |
| 431_I | 491_W | 1.457 | 1.00 |
| 430_V | 522_F | 1.446 | 1.00 |
| 83_I | 89_F | 1.445 | 1.00 |
| 230_F | 255_I | 1.438 | 1.00 |
| 237_L | 267_I | 1.436 | 1.00 |
| 70_P | 79_S | 1.426 | 1.00 |
| 148_Q | 152_K | 1.425 | 1.00 |
| 15_W | 26_G | 1.422 | 1.00 |
| 25_M | 115_L | 1.415 | 1.00 |
| 58_V | 76_A | 1.412 | 1.00 |
| 12_Y | 23_I | 1.408 | 1.00 |
| 411_E | 414_R | 1.404 | 0.99 |
| 57_V | 110_Q | 1.401 | 0.99 |
| 173_L | 295_T | 1.390 | 0.99 |
| 505_A | 519_G | 1.379 | 0.99 |
| 35_T | 139_N | 1.378 | 0.99 |
| 276_N | 298_N | 1.374 | 0.99 |
| 16_A | 21_E | 1.371 | 0.99 |
| 45_V | 90_F | 1.370 | 0.99 |
| 40_A | 124_P | 1.369 | 0.99 |
| 511_E | 514_L | 1.363 | 0.99 |
| 448_W | 518_M | 1.361 | 0.99 |
| 232_I | 237_L | 1.346 | 0.99 |
| 424_I | 525_G | 1.344 | 0.99 |
| 255_I | 263_Y | 1.342 | 0.99 |
| 209_V | 297_L | 1.332 | 0.99 |
| 118_S | 141_L | 1.329 | 0.99 |
| 3_I | 43_S | 1.314 | 0.99 |
| 171_I | 293_G | 1.313 | 0.99 |
| 227_A | 485_V | 1.312 | 0.99 |
| 474_N | 477_N | 1.310 | 0.99 |
| 105_G | 110_Q | 1.308 | 0.99 |
| 320_D | 323_R | 1.304 | 0.99 |
| 208_H | 300_L | 1.302 | 0.99 |
| 12_Y | 53_H | 1.301 | 0.99 |
| 456_W | 480_L | 1.299 | 0.99 |
| 24_V | 145_W | 1.297 | 0.99 |
| 450_L | 496_F | 1.297 | 0.99 |
| 507_H | 513_H | 1.293 | 0.99 |
| 428_V | 496_F | 1.280 | 0.99 |
| 409_T | 414_R | 1.273 | 0.99 |
| 452_G | 504_W | 1.267 | 0.99 |
| 422_F | 498_A | 1.264 | 0.99 |
| 450_L | 506_F | 1.262 | 0.99 |
| 410_N | 414_R | 1.260 | 0.99 |
| 64_I | 121_V | 1.259 | 0.99 |
| 323_R | 327_F | 1.257 | 0.99 |
| 331_I | 334_A | 1.252 | 0.99 |
| 459_G | 493_A | 1.251 | 0.99 |
| 376_P | 381_M | 1.251 | 0.99 |
| 113_A | 150_I | 1.247 | 0.99 |
| 80_Q | 89_F | 1.247 | 0.99 |
| 446_H | 509_H | 1.246 | 0.98 |
| 327_F | 330_R | 1.244 | 0.98 |
| 328_T | 331_I | 1.242 | 0.98 |
| 448_W | 506_F | 1.241 | 0.98 |
| 8_W | 36_I | 1.239 | 0.98 |
| 100_T | 218_R | 1.226 | 0.98 |
| 209_V | 215_Y | 1.224 | 0.98 |
| 27_I | 115_L | 1.224 | 0.98 |
| 330_R | 334_A | 1.222 | 0.98 |
| 342_V | 345_N | 1.217 | 0.98 |
| 514_L | 519_G | 1.214 | 0.98 |
| 10_V | 48_L | 1.214 | 0.98 |
| 196_L | 199_S | 1.210 | 0.98 |
| 76_A | 107_L | 1.207 | 0.98 |
| 365_I | 370_L | 1.205 | 0.98 |
| 378_L | 381_M | 1.204 | 0.98 |
| 346_R | 349_F | 1.204 | 0.98 |
| 131_P | 246_Y | 1.201 | 0.98 |
| 353_T | 356_V | 1.201 | 0.98 |
| 136_G | 215_Y | 1.198 | 0.98 |
| 209_V | 267_I | 1.198 | 0.98 |
| 336_G | 339_K | 1.194 | 0.98 |
| 242_A | 247_V | 1.190 | 0.98 |
| 336_G | 340_P | 1.188 | 0.98 |
| 457_V | 492_T | 1.185 | 0.98 |
| 142_L | 230_F | 1.182 | 0.98 |
| 346_R | 426_E | 1.176 | 0.98 |
| 455_F | 484_V | 1.173 | 0.98 |
| 329_Y | 335_M | 1.172 | 0.98 |
| 295_T | 299_Y | 1.167 | 0.97 |
| 457_V | 484_V | 1.156 | 0.97 |
| 239_V | 252_T | 1.154 | 0.97 |
| 527_E | 530_G | 1.151 | 0.97 |
| 362_K | 365_I | 1.150 | 0.97 |
| 422_F | 428_V | 1.146 | 0.97 |
| 328_T | 332_T | 1.138 | 0.97 |
| 328_T | 334_A | 1.136 | 0.97 |
| 332_T | 335_M | 1.132 | 0.97 |
| 333_A | 336_G | 1.129 | 0.97 |
| 38_A | 95_V | 1.126 | 0.97 |
| 326_N | 329_Y | 1.124 | 0.97 |
| 329_Y | 332_T | 1.124 | 0.97 |
| 247_V | 250_F | 1.123 | 0.97 |
| 324_S | 328_T | 1.122 | 0.97 |
| 272_N | 275_E | 1.121 | 0.97 |
| 366_N | 370_L | 1.119 | 0.96 |
| 273_P | 277_Y | 1.113 | 0.96 |
| 298_N | 302_N | 1.110 | 0.96 |
| 93_F | 119_L | 1.109 | 0.96 |
| 366_N | 369_S | 1.108 | 0.96 |
| 347_R | 429_D | 1.107 | 0.96 |
| 48_L | 59_I | 1.104 | 0.96 |
| 446_H | 508_C | 1.099 | 0.96 |
| 466_S | 469_E | 1.096 | 0.96 |
| 446_H | 513_H | 1.094 | 0.96 |
| 235_H | 299_Y | 1.089 | 0.96 |
| 377_Y | 381_M | 1.087 | 0.96 |
| 332_T | 336_G | 1.087 | 0.96 |
| 106_H | 509_H | 1.084 | 0.96 |
| 25_M | 113_A | 1.081 | 0.95 |
| 219_I | 257_I | 1.081 | 0.95 |
| 10_V | 27_I | 1.080 | 0.95 |
| 146_W | 153_Q | 1.072 | 0.95 |
| 503_V | 521_V | 1.067 | 0.95 |
| 235_H | 277_Y | 1.066 | 0.95 |
| 315_T | 318_W | 1.061 | 0.95 |
| 450_L | 455_F | 1.059 | 0.95 |
| 294_L | 297_L | 1.058 | 0.95 |
| 314_Q | 317_A | 1.055 | 0.95 |
| 217_I | 267_I | 1.051 | 0.94 |
| 299_Y | 303_S | 1.048 | 0.94 |
| 332_T | 337_S | 1.048 | 0.94 |
| 46_V | 61_W | 1.048 | 0.94 |
| 20_N | 147_H | 1.047 | 0.94 |
| 365_I | 368_V | 1.043 | 0.94 |
| 241_E | 246_Y | 1.041 | 0.94 |
| 106_H | 508_C | 1.039 | 0.94 |
| 421_Q | 523_A | 1.039 | 0.94 |
| 377_Y | 521_V | 1.038 | 0.94 |
| 506_F | 522_F | 1.034 | 0.94 |
| 44_V | 93_F | 1.031 | 0.94 |
| 274_S | 301_P | 1.031 | 0.94 |
| 317_A | 320_D | 1.027 | 0.94 |
| 39_N | 42_D | 1.025 | 0.93 |
| 78_I | 505_A | 1.024 | 0.93 |
| 65_L | 454_D | 1.023 | 0.93 |
| 75_T | 79_S | 1.021 | 0.93 |
| 278_W | 296_L | 1.021 | 0.93 |
| 430_V | 496_F | 1.019 | 0.93 |
| 62_H | 509_H | 1.019 | 0.93 |
| 69_T | 72_A | 1.019 | 0.93 |
| 118_S | 139_N | 1.018 | 0.93 |
| 62_H | 508_C | 1.018 | 0.93 |
| 385_L | 388_A | 1.016 | 0.93 |
| 192_E | 195_K | 1.015 | 0.93 |
| 128_K | 266_L | 1.015 | 0.93 |
| 359_G | 362_K | 1.015 | 0.93 |
| 218_R | 264_S | 1.015 | 0.93 |
| 39_N | 124_P | 1.012 | 0.93 |
| 401_D | 404_I | 1.011 | 0.93 |
| 128_K | 131_P | 1.003 | 0.93 |
| 35_T | 120_I | 1.003 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1aozA | 2 | 0.9945 | 100 | 0.138 | Contact Map |
| 1zpuA | 6 | 0.8764 | 100 | 0.219 | Contact Map |
| 3sqrA | 1 | 0.8856 | 100 | 0.225 | Contact Map |
| 2q9oA | 2 | 0.8856 | 100 | 0.241 | Contact Map |
| 1v10A | 1 | 0.8579 | 100 | 0.254 | Contact Map |
| 3pxlA | 1 | 0.8653 | 100 | 0.257 | Contact Map |
| 3t6vA | 3 | 0.8708 | 100 | 0.257 | Contact Map |
| 1hfuA | 1 | 0.8561 | 100 | 0.259 | Contact Map |
| 2xu9A | 1 | 0.7417 | 100 | 0.325 | Contact Map |
| 3gyrA | 12 | 0.8782 | 100 | 0.329 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)