| ABCG5 |
| ID: | 1479323760 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 651 (610) | ||
| Sequences: | 5523 (3051.4) | ||
| Seq/Len: | 9.054 | ||
| Nf(neff/√len): | 123.5 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 9.054).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 42_H | 71_D | 4.085 | 1.00 |
| 145_R | 181_A | 3.873 | 1.00 |
| 173_A | 178_S | 3.773 | 1.00 |
| 327_S | 331_K | 3.653 | 1.00 |
| 80_Q | 262_K | 3.556 | 1.00 |
| 70_K | 270_E | 3.548 | 1.00 |
| 401_L | 489_T | 3.368 | 1.00 |
| 236_V | 240_R | 3.297 | 1.00 |
| 402_F | 405_L | 3.268 | 1.00 |
| 496_V | 500_G | 3.181 | 1.00 |
| 505_A | 574_I | 3.039 | 1.00 |
| 581_Y | 606_I | 2.897 | 1.00 |
| 145_R | 182_D | 2.860 | 1.00 |
| 185_I | 201_V | 2.858 | 1.00 |
| 282_D | 286_D | 2.708 | 1.00 |
| 67_Q | 70_K | 2.691 | 1.00 |
| 286_D | 334_I | 2.686 | 1.00 |
| 480_M | 503_S | 2.618 | 1.00 |
| 77_E | 341_N | 2.549 | 1.00 |
| 364_V | 461_W | 2.513 | 1.00 |
| 44_S | 71_D | 2.456 | 1.00 |
| 46_S | 67_Q | 2.407 | 1.00 |
| 282_D | 285_N | 2.357 | 1.00 |
| 76_V | 82_M | 2.334 | 1.00 |
| 519_V | 637_L | 2.322 | 1.00 |
| 626_I | 629_S | 2.319 | 1.00 |
| 44_S | 67_Q | 2.317 | 1.00 |
| 175_L | 204_A | 2.292 | 1.00 |
| 40_I | 74_L | 2.256 | 1.00 |
| 186_G | 192_G | 2.246 | 1.00 |
| 333_A | 336_H | 2.226 | 1.00 |
| 149_H | 165_Q | 2.225 | 1.00 |
| 573_E | 619_R | 2.184 | 1.00 |
| 285_N | 290_P | 2.174 | 1.00 |
| 422_L | 490_L | 2.163 | 1.00 |
| 68_I | 94_T | 2.159 | 1.00 |
| 335_C | 339_L | 2.152 | 1.00 |
| 362_P | 366_S | 2.118 | 1.00 |
| 560_K | 563_S | 2.103 | 1.00 |
| 280_M | 301_Y | 2.086 | 1.00 |
| 394_L | 485_V | 2.070 | 1.00 |
| 422_L | 492_L | 2.039 | 1.00 |
| 624_F | 628_Y | 2.035 | 1.00 |
| 495_E | 498_R | 2.030 | 1.00 |
| 422_L | 486_C | 2.009 | 1.00 |
| 286_D | 333_A | 2.004 | 1.00 |
| 143_T | 146_E | 1.992 | 1.00 |
| 508_A | 624_F | 1.972 | 1.00 |
| 273_F | 276_T | 1.971 | 1.00 |
| 501_Y | 574_I | 1.968 | 1.00 |
| 193_I | 201_V | 1.965 | 1.00 |
| 488_W | 494_P | 1.965 | 1.00 |
| 147_T | 185_I | 1.948 | 1.00 |
| 483_S | 502_F | 1.947 | 1.00 |
| 77_E | 80_Q | 1.926 | 1.00 |
| 504_A | 624_F | 1.924 | 1.00 |
| 419_R | 579_E | 1.921 | 1.00 |
| 493_H | 498_R | 1.900 | 1.00 |
| 148_L | 204_A | 1.883 | 1.00 |
| 484_S | 499_F | 1.866 | 1.00 |
| 373_R | 377_R | 1.856 | 1.00 |
| 333_A | 337_K | 1.854 | 1.00 |
| 364_V | 368_L | 1.836 | 1.00 |
| 279_E | 282_D | 1.830 | 1.00 |
| 405_L | 489_T | 1.815 | 1.00 |
| 239_A | 245_V | 1.812 | 1.00 |
| 402_F | 489_T | 1.799 | 1.00 |
| 290_P | 300_F | 1.786 | 1.00 |
| 558_P | 561_I | 1.771 | 1.00 |
| 476_V | 507_L | 1.766 | 1.00 |
| 287_C | 328_A | 1.750 | 1.00 |
| 369_G | 373_R | 1.739 | 1.00 |
| 142_L | 147_T | 1.738 | 1.00 |
| 41_L | 112_E | 1.733 | 1.00 |
| 387_I | 391_L | 1.732 | 1.00 |
| 180_V | 183_R | 1.724 | 1.00 |
| 73_S | 342_I | 1.713 | 1.00 |
| 509_P | 571_C | 1.712 | 1.00 |
| 555_M | 560_K | 1.703 | 1.00 |
| 292_P | 295_S | 1.701 | 1.00 |
| 112_E | 119_A | 1.700 | 1.00 |
| 463_M | 467_Y | 1.700 | 1.00 |
| 153_L | 165_Q | 1.697 | 1.00 |
| 604_Q | 607_Q | 1.693 | 1.00 |
| 169_E | 181_A | 1.693 | 1.00 |
| 557_I | 560_K | 1.691 | 1.00 |
| 264_A | 271_L | 1.688 | 1.00 |
| 408_R | 414_G | 1.665 | 1.00 |
| 508_A | 628_Y | 1.664 | 1.00 |
| 633_A | 637_L | 1.648 | 1.00 |
| 146_E | 381_R | 1.634 | 1.00 |
| 406_R | 494_P | 1.624 | 1.00 |
| 366_S | 370_V | 1.612 | 1.00 |
| 337_K | 340_K | 1.608 | 1.00 |
| 139_L | 448_V | 1.603 | 1.00 |
| 82_M | 262_K | 1.597 | 1.00 |
| 281_L | 291_C | 1.592 | 1.00 |
| 153_L | 162_G | 1.582 | 1.00 |
| 98_A | 103_L | 1.578 | 1.00 |
| 145_R | 169_E | 1.569 | 1.00 |
| 41_L | 343_E | 1.563 | 1.00 |
| 505_A | 624_F | 1.559 | 1.00 |
| 371_L | 462_Q | 1.542 | 1.00 |
| 272_I | 305_T | 1.540 | 1.00 |
| 582_G | 606_I | 1.534 | 1.00 |
| 607_Q | 610_E | 1.527 | 1.00 |
| 330_K | 335_C | 1.525 | 1.00 |
| 329_Y | 334_I | 1.524 | 1.00 |
| 157_R | 160_N | 1.521 | 1.00 |
| 486_C | 502_F | 1.514 | 1.00 |
| 397_G | 485_V | 1.499 | 1.00 |
| 281_L | 297_P | 1.491 | 1.00 |
| 563_S | 569_K | 1.490 | 1.00 |
| 619_R | 622_M | 1.483 | 1.00 |
| 619_R | 623_N | 1.480 | 1.00 |
| 97_D | 131_Y | 1.477 | 1.00 |
| 573_E | 577_V | 1.472 | 1.00 |
| 552_I | 563_S | 1.462 | 1.00 |
| 220_T | 228_A | 1.461 | 1.00 |
| 36_H | 116_N | 1.455 | 1.00 |
| 150_Y | 154_L | 1.448 | 1.00 |
| 74_L | 99_M | 1.446 | 1.00 |
| 584_N | 604_Q | 1.446 | 1.00 |
| 283_F | 287_C | 1.444 | 0.99 |
| 501_Y | 620_F | 1.443 | 0.99 |
| 262_K | 276_T | 1.435 | 0.99 |
| 232_V | 235_L | 1.431 | 0.99 |
| 368_L | 372_L | 1.424 | 0.99 |
| 483_S | 503_S | 1.421 | 0.99 |
| 629_S | 633_A | 1.408 | 0.99 |
| 407_V | 419_R | 1.403 | 0.99 |
| 552_I | 560_K | 1.401 | 0.99 |
| 100_S | 215_L | 1.395 | 0.99 |
| 610_E | 616_A | 1.392 | 0.99 |
| 145_R | 149_H | 1.388 | 0.99 |
| 501_Y | 578_N | 1.386 | 0.99 |
| 149_H | 153_L | 1.384 | 0.99 |
| 488_W | 496_V | 1.383 | 0.99 |
| 515_F | 635_V | 1.381 | 0.99 |
| 485_V | 489_T | 1.377 | 0.99 |
| 187_N | 190_L | 1.376 | 0.99 |
| 71_D | 330_K | 1.367 | 0.99 |
| 270_E | 326_E | 1.366 | 0.99 |
| 440_N | 443_P | 1.356 | 0.99 |
| 97_D | 103_L | 1.356 | 0.99 |
| 149_H | 169_E | 1.335 | 0.99 |
| 628_Y | 632_P | 1.334 | 0.99 |
| 452_E | 457_L | 1.330 | 0.99 |
| 179_H | 197_E | 1.329 | 0.99 |
| 152_A | 156_I | 1.326 | 0.99 |
| 271_L | 274_C | 1.325 | 0.99 |
| 398_L | 402_F | 1.325 | 0.99 |
| 622_M | 625_L | 1.324 | 0.99 |
| 479_T | 507_L | 1.324 | 0.99 |
| 502_F | 578_N | 1.322 | 0.99 |
| 372_L | 376_T | 1.317 | 0.99 |
| 406_R | 491_G | 1.314 | 0.99 |
| 340_K | 344_R | 1.310 | 0.99 |
| 172_M | 181_A | 1.308 | 0.99 |
| 622_M | 626_I | 1.307 | 0.99 |
| 368_L | 469_L | 1.305 | 0.99 |
| 491_G | 494_P | 1.303 | 0.99 |
| 126_Q | 456_G | 1.302 | 0.99 |
| 153_L | 373_R | 1.300 | 0.99 |
| 576_V | 609_I | 1.294 | 0.99 |
| 617_T | 620_F | 1.287 | 0.99 |
| 98_A | 109_F | 1.287 | 0.99 |
| 540_A | 562_I | 1.285 | 0.99 |
| 364_V | 462_Q | 1.285 | 0.99 |
| 376_T | 470_H | 1.283 | 0.99 |
| 394_L | 481_I | 1.283 | 0.99 |
| 497_A | 500_G | 1.282 | 0.99 |
| 45_Y | 98_A | 1.276 | 0.98 |
| 380_V | 383_K | 1.272 | 0.98 |
| 281_L | 285_N | 1.271 | 0.98 |
| 289_Y | 300_F | 1.259 | 0.98 |
| 48_S | 65_T | 1.254 | 0.98 |
| 72_V | 266_L | 1.254 | 0.98 |
| 90_S | 248_T | 1.241 | 0.98 |
| 587_C | 600_C | 1.231 | 0.98 |
| 484_S | 488_W | 1.228 | 0.98 |
| 329_Y | 338_T | 1.224 | 0.98 |
| 276_T | 279_E | 1.223 | 0.98 |
| 253_R | 303_D | 1.222 | 0.98 |
| 43_A | 113_V | 1.216 | 0.98 |
| 86_G | 91_G | 1.215 | 0.98 |
| 402_F | 406_R | 1.215 | 0.98 |
| 196_G | 200_R | 1.213 | 0.98 |
| 194_S | 197_E | 1.212 | 0.98 |
| 144_V | 201_V | 1.210 | 0.98 |
| 178_S | 181_A | 1.209 | 0.98 |
| 82_M | 246_V | 1.208 | 0.98 |
| 264_A | 274_C | 1.204 | 0.98 |
| 72_V | 95_L | 1.204 | 0.98 |
| 146_E | 377_R | 1.202 | 0.97 |
| 81_I | 239_A | 1.202 | 0.97 |
| 144_V | 193_I | 1.197 | 0.97 |
| 129_F | 215_L | 1.195 | 0.97 |
| 194_S | 200_R | 1.191 | 0.97 |
| 332_S | 335_C | 1.186 | 0.97 |
| 326_E | 330_K | 1.183 | 0.97 |
| 397_G | 482_F | 1.179 | 0.97 |
| 42_H | 339_L | 1.178 | 0.97 |
| 479_T | 503_S | 1.176 | 0.97 |
| 221_T | 250_H | 1.174 | 0.97 |
| 458_Y | 462_Q | 1.168 | 0.97 |
| 82_M | 274_C | 1.168 | 0.97 |
| 257_F | 277_P | 1.167 | 0.97 |
| 464_M | 467_Y | 1.164 | 0.97 |
| 419_R | 583_L | 1.156 | 0.97 |
| 365_F | 368_L | 1.153 | 0.97 |
| 172_M | 178_S | 1.152 | 0.97 |
| 280_M | 284_F | 1.147 | 0.96 |
| 73_S | 335_C | 1.145 | 0.96 |
| 263_I | 277_P | 1.143 | 0.96 |
| 41_L | 339_L | 1.141 | 0.96 |
| 91_G | 94_T | 1.137 | 0.96 |
| 177_L | 180_V | 1.134 | 0.96 |
| 363_G | 366_S | 1.133 | 0.96 |
| 193_I | 198_R | 1.130 | 0.96 |
| 138_L | 205_A | 1.126 | 0.96 |
| 82_M | 264_A | 1.126 | 0.96 |
| 520_L | 524_V | 1.125 | 0.96 |
| 428_G | 432_Y | 1.124 | 0.96 |
| 508_A | 631_I | 1.124 | 0.96 |
| 334_I | 338_T | 1.121 | 0.96 |
| 138_L | 201_V | 1.119 | 0.96 |
| 218_E | 221_T | 1.118 | 0.96 |
| 153_L | 164_F | 1.117 | 0.96 |
| 72_V | 271_L | 1.117 | 0.96 |
| 154_L | 362_P | 1.114 | 0.96 |
| 570_Y | 573_E | 1.113 | 0.96 |
| 429_A | 434_G | 1.113 | 0.96 |
| 270_E | 330_K | 1.111 | 0.95 |
| 82_M | 244_I | 1.109 | 0.95 |
| 576_V | 580_F | 1.109 | 0.95 |
| 45_Y | 107_G | 1.109 | 0.95 |
| 327_S | 330_K | 1.103 | 0.95 |
| 273_F | 283_F | 1.099 | 0.95 |
| 93_T | 97_D | 1.099 | 0.95 |
| 343_E | 347_H | 1.098 | 0.95 |
| 279_E | 337_K | 1.096 | 0.95 |
| 497_A | 501_Y | 1.095 | 0.95 |
| 398_L | 405_L | 1.093 | 0.95 |
| 49_H | 52_R | 1.093 | 0.95 |
| 501_Y | 505_A | 1.092 | 0.95 |
| 172_M | 204_A | 1.092 | 0.95 |
| 339_L | 343_E | 1.089 | 0.95 |
| 301_Y | 305_T | 1.087 | 0.95 |
| 238_L | 245_V | 1.086 | 0.95 |
| 115_V | 125_F | 1.084 | 0.95 |
| 86_G | 89_G | 1.079 | 0.94 |
| 468_A | 515_F | 1.078 | 0.94 |
| 254_S | 258_Q | 1.078 | 0.94 |
| 472_L | 507_L | 1.076 | 0.94 |
| 556_P | 559_F | 1.076 | 0.94 |
| 475_S | 478_A | 1.075 | 0.94 |
| 85_L | 263_I | 1.075 | 0.94 |
| 236_V | 261_D | 1.074 | 0.94 |
| 343_E | 346_K | 1.073 | 0.94 |
| 368_L | 465_L | 1.071 | 0.94 |
| 99_M | 215_L | 1.070 | 0.94 |
| 630_F | 634_L | 1.069 | 0.94 |
| 487_Y | 492_L | 1.065 | 0.94 |
| 526_N | 529_I | 1.063 | 0.94 |
| 571_C | 627_L | 1.062 | 0.94 |
| 273_F | 279_E | 1.059 | 0.94 |
| 329_Y | 332_S | 1.056 | 0.94 |
| 621_T | 625_L | 1.055 | 0.94 |
| 636_I | 640_V | 1.054 | 0.94 |
| 581_Y | 617_T | 1.053 | 0.94 |
| 235_L | 245_V | 1.049 | 0.93 |
| 328_A | 331_K | 1.049 | 0.93 |
| 502_F | 575_L | 1.045 | 0.93 |
| 426_F | 502_F | 1.045 | 0.93 |
| 112_E | 346_K | 1.044 | 0.93 |
| 331_K | 336_H | 1.044 | 0.93 |
| 493_H | 583_L | 1.043 | 0.93 |
| 551_N | 554_E | 1.041 | 0.93 |
| 73_S | 339_L | 1.041 | 0.93 |
| 83_C | 249_I | 1.040 | 0.93 |
| 147_T | 151_T | 1.035 | 0.93 |
| 336_H | 340_K | 1.033 | 0.93 |
| 512_I | 631_I | 1.032 | 0.93 |
| 637_L | 641_V | 1.031 | 0.93 |
| 149_H | 168_V | 1.029 | 0.92 |
| 92_K | 218_E | 1.029 | 0.92 |
| 187_N | 191_G | 1.024 | 0.92 |
| 626_I | 633_A | 1.024 | 0.92 |
| 95_L | 99_M | 1.024 | 0.92 |
| 505_A | 571_C | 1.018 | 0.92 |
| 138_L | 202_S | 1.015 | 0.92 |
| 89_G | 92_K | 1.012 | 0.92 |
| 548_F | 572_S | 1.012 | 0.92 |
| 134_Q | 218_E | 1.011 | 0.92 |
| 341_N | 344_R | 1.010 | 0.92 |
| 166_K | 170_A | 1.007 | 0.91 |
| 140_S | 187_N | 1.007 | 0.91 |
| 603_T | 607_Q | 1.005 | 0.91 |
| 572_S | 576_V | 1.005 | 0.91 |
| 134_Q | 221_T | 1.005 | 0.91 |
| 210_D | 243_R | 1.003 | 0.91 |
| 605_G | 609_I | 1.001 | 0.91 |
| 91_G | 96_L | 1.001 | 0.91 |
| 267_S | 272_I | 1.000 | 0.91 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4rvcA | 1 | 0.3656 | 100 | 0.744 | Contact Map |
| 4u00A | 1 | 0 | 100 | 0.746 | Contact Map |
| 2oljA | 2 | 0.3641 | 100 | 0.746 | Contact Map |
| 1vplA | 1 | 0.3625 | 100 | 0.747 | Contact Map |
| 4ymuJ | 2 | 0 | 100 | 0.747 | Contact Map |
| 4mkiB | 2 | 0.4194 | 100 | 0.747 | Contact Map |
| 4huqA | 1 | 0.4147 | 100 | 0.748 | Contact Map |
| 4huqB | 1 | 0.4101 | 100 | 0.748 | Contact Map |
| 4hluD | 2 | 0.381 | 100 | 0.748 | Contact Map |
| 2yz2A | 2 | 0.4025 | 100 | 0.75 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)