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AtRPM1

ID: 1470828282 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 926 (880)
Sequences: 3378 (2396.4)
Seq/Len: 3.839
Nf(neff/√len): 80.8

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.839).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
198_V210_S3.5061.00
317_M332_E3.2561.00
423_S427_L3.2431.00
654_Y688_V3.2011.00
126_Q130_D3.1771.00
424_F427_L3.1181.00
341_A380_I3.0151.00
363_E367_R2.9441.00
471_E653_R2.8841.00
424_F428_P2.8701.00
806_V829_K2.8531.00
853_Q877_Q2.8231.00
368_K371_E2.6421.00
390_K395_E2.5941.00
829_K853_Q2.5761.00
314_T320_A2.5691.00
338_E371_E2.5611.00
759_I762_L2.5161.00
825_F828_L2.4621.00
612_S633_N2.4281.00
833_I836_M2.3821.00
421_F425_N2.3601.00
876_L879_L2.3351.00
735_R760_E2.3301.00
860_C863_L2.3221.00
420_M424_F2.3151.00
224_E279_K2.2681.00
43_S66_T2.2541.00
447_M501_F2.2211.00
230_T444_N2.2201.00
46_E62_Q2.2181.00
420_M423_S2.1751.00
220_R226_Y2.1591.00
420_M427_L2.1341.00
180_G184_G2.0971.00
805_L808_L2.0891.00
66_T70_N2.0641.00
211_A215_K2.0261.00
342_W367_R2.0251.00
457_M462_V2.0251.00
423_S428_P2.0041.00
459_Q544_H1.9491.00
626_L629_L1.9361.00
314_T319_V1.9311.00
686_L689_M1.9251.00
828_L831_L1.9191.00
341_A344_L1.9191.00
313_T319_V1.8971.00
342_W346_S1.8931.00
180_G218_S1.8931.00
64_F67_F1.8841.00
420_M428_P1.8831.00
453_I474_A1.8831.00
397_K401_S1.8741.00
35_K39_L1.8691.00
755_A774_W1.8511.00
419_I423_S1.8491.00
451_R587_D1.8441.00
202_G505_D1.8431.00
36_K70_N1.8401.00
202_G315_R1.8401.00
852_L855_L1.8271.00
435_F511_A1.8160.99
449_R475_D1.8060.99
683_L689_M1.8000.99
449_R478_L1.7830.99
473_V477_Y1.7820.99
229_V244_M1.7750.99
130_D134_R1.7710.99
201_M376_L1.7610.99
416_V427_L1.7570.99
419_I424_F1.7500.99
624_H645_G1.7280.99
131_S134_R1.7190.99
652_L655_L1.7110.99
418_S424_F1.7000.99
315_R423_S1.6990.99
438_C487_L1.6850.99
286_D289_W1.6840.99
807_W830_I1.6840.99
423_S426_D1.6810.99
431_L477_Y1.6690.99
366_A369_L1.6640.99
629_L632_L1.6580.99
315_R319_V1.6540.99
76_Y80_D1.6490.99
630_E651_K1.6450.99
313_T320_A1.6340.99
48_T51_H1.6230.99
417_R424_F1.6150.99
626_L632_L1.6130.99
621_K645_G1.6100.99
833_I839_L1.6070.98
628_N651_K1.5950.98
633_N656_I1.5870.98
849_M852_L1.5850.98
428_P431_L1.5840.98
416_V423_S1.5790.98
266_R270_E1.5780.98
337_K340_E1.5780.98
443_V508_W1.5780.98
416_V424_F1.5760.98
422_L427_L1.5740.98
212_N215_K1.5600.98
857_V860_C1.5500.98
422_L426_D1.5480.98
817_P840_T1.5460.98
441_F455_M1.5460.98
30_E119_G1.5420.98
480_E484_R1.5420.98
422_L428_P1.5400.98
227_A283_V1.5330.98
362_L366_A1.5300.98
203_G206_K1.5290.98
641_E675_R1.5230.98
370_V380_I1.5170.98
816_G819_L1.5160.98
419_I427_L1.5130.98
238_E265_Y1.5060.97
47_D51_H1.5050.97
77_Q124_M1.5050.97
47_D61_T1.5030.97
344_L380_I1.5000.97
67_F71_T1.4980.97
200_G205_G1.4980.97
441_F451_R1.4970.97
350_F357_C1.4970.97
469_K472_E1.4910.97
340_E343_V1.4900.97
446_R502_K1.4850.97
178_P331_H1.4810.97
76_Y83_D1.4740.97
200_G203_G1.4740.97
416_V420_M1.4720.97
635_K656_I1.4720.97
1_M6_V1.4720.97
505_D509_E1.4660.97
202_G504_H1.4650.97
39_L69_A1.4640.97
478_L501_F1.4620.97
362_L365_I1.4600.97
708_L711_I1.4580.97
287_D444_N1.4570.97
33_K127_S1.4540.97
39_L66_T1.4500.97
457_M470_A1.4420.96
784_L808_L1.4300.96
442_P445_Y1.4270.96
603_M606_L1.4260.96
202_G289_W1.4250.96
337_K341_A1.4230.96
369_L383_L1.4230.96
376_L379_A1.4220.96
80_D562_H1.4210.96
393_E397_K1.4210.96
840_T864_E1.4120.96
587_D610_N1.4040.96
421_F427_L1.4000.96
623_F629_L1.3970.95
20_E112_H1.3970.95
37_E41_M1.3960.95
311_M314_T1.3870.95
459_Q585_A1.3860.95
227_A244_M1.3860.95
422_L425_N1.3860.95
736_F761_K1.3850.95
349_A384_G1.3840.95
654_Y687_Q1.3830.95
437_Y459_Q1.3780.95
231_I240_V1.3780.95
313_T317_M1.3780.95
380_I384_G1.3780.95
508_W512_L1.3750.95
178_P181_K1.3750.95
44_F68_V1.3750.95
203_G207_T1.3740.95
289_W505_D1.3740.95
701_N705_M1.3730.95
503_M511_A1.3730.95
280_R308_S1.3690.95
48_T68_V1.3610.95
311_M315_R1.3560.94
236_V239_D1.3560.94
630_E653_R1.3470.94
183_I216_S1.3400.94
227_A247_E1.3380.94
127_S131_S1.3360.94
368_K400_Y1.3330.94
208_T505_D1.3310.94
26_G30_E1.3170.93
204_S485_N1.3130.93
271_K274_E1.3080.93
471_E687_Q1.3030.93
610_N614_T1.3000.93
228_W284_V1.2970.93
205_G209_L1.2960.93
204_S333_I1.2920.92
813_A816_G1.2900.92
282_I311_M1.2900.92
342_W366_A1.2900.92
802_L805_L1.2900.92
37_E135_Y1.2860.92
290_T293_L1.2850.92
129_S132_M1.2800.92
425_N428_P1.2770.92
734_I737_L1.2720.92
129_S134_R1.2710.92
70_N131_S1.2680.91
241_F268_L1.2650.91
281_Y302_P1.2630.91
683_L686_L1.2620.91
446_R490_I1.2600.91
313_T423_S1.2590.91
313_T316_D1.2570.91
639_I642_L1.2540.91
214_F227_A1.2530.91
8_F11_G1.2530.91
606_L609_L1.2510.91
428_P477_Y1.2510.91
610_N633_N1.2500.91
468_V476_S1.2500.91
584_R605_N1.2490.91
2_A5_T1.2490.91
208_T231_I1.2480.91
183_I213_I1.2480.91
313_T318_N1.2450.90
244_M297_I1.2440.90
863_L867_P1.2430.90
276_L302_P1.2410.90
239_D243_T1.2400.90
430_P477_Y1.2360.90
421_F424_F1.2340.90
30_E33_K1.2340.90
336_L344_L1.2320.90
454_R631_T1.2310.90
399_V402_T1.2300.90
214_F226_Y1.2290.90
421_F426_D1.2270.90
312_M319_V1.2260.90
542_S545_L1.2250.89
805_L810_F1.2240.89
838_H862_G1.2240.89
228_W235_Y1.2210.89
200_G206_K1.2210.89
830_I854_K1.2200.89
437_Y441_F1.2200.89
179_K218_S1.2180.89
869_G876_L1.2180.89
231_I289_W1.2180.89
361_N393_E1.2170.89
177_A181_K1.2170.89
313_T424_F1.2160.89
458_A631_T1.2140.89
243_T247_E1.2130.89
345_F370_V1.2120.89
601_V622_N1.2080.89
373_C376_L1.2070.89
562_H584_R1.2010.88
379_A484_R1.1990.88
486_M507_I1.1980.88
88_H91_G1.1970.88
39_L70_N1.1970.88
444_N505_D1.1970.88
281_Y308_S1.1970.88
423_S435_F1.1880.87
307_G310_V1.1880.87
289_W315_R1.1850.87
232_S289_W1.1840.87
187_L196_V1.1830.87
891_R905_H1.1800.87
613_K636_H1.1790.87
40_I131_S1.1760.87
478_L482_V1.1760.87
264_G267_E1.1720.86
206_K209_L1.1710.86
33_K122_N1.1690.86
840_T862_G1.1690.86
270_E274_E1.1680.86
201_M204_S1.1680.86
852_L863_L1.1670.86
40_I63_L1.1670.86
876_L881_L1.1660.86
202_G287_D1.1600.86
869_G873_L1.1590.86
838_H841_E1.1540.85
81_I121_V1.1540.85
233_K443_V1.1490.85
231_I235_Y1.1450.85
227_A248_F1.1410.84
854_K878_E1.1400.84
67_F132_M1.1390.84
446_R500_A1.1390.84
125_I131_S1.1340.84
884_V888_L1.1340.84
375_G378_L1.1330.84
30_E122_N1.1310.84
688_V710_R1.1310.84
420_M425_N1.1300.84
781_L784_L1.1280.84
336_L345_F1.1270.83
15_S19_N1.1260.83
362_L369_L1.1180.83
287_D315_R1.1180.83
418_S423_S1.1180.83
72_R80_D1.1170.83
498_P501_F1.1160.83
197_A330_K1.1150.83
345_F350_F1.1130.82
869_G872_N1.1110.82
863_L866_V1.1100.82
74_L78_I1.1100.82
431_L480_E1.1050.82
444_N502_K1.1050.82
506_V510_I1.1040.82
900_R907_P1.1020.82
269_V273_V1.1020.82
586_L603_M1.1010.81
418_S425_N1.0980.81
295_R298_S1.0970.81
452_L501_F1.0970.81
184_G188_S1.0970.81
32_D70_N1.0960.81
34_M44_F1.0930.81
455_M459_Q1.0930.81
32_D35_K1.0930.81
888_L892_I1.0900.81
726_D731_I1.0900.81
20_E27_V1.0900.81
182_L213_I1.0890.80
202_G420_M1.0890.80
311_M427_L1.0880.80
785_G809_S1.0830.80
447_M502_K1.0830.80
315_R320_A1.0830.80
36_K131_S1.0830.80
164_S167_F1.0800.80
47_D63_L1.0790.80
48_T67_F1.0780.80
418_S421_F1.0760.79
48_T64_F1.0760.79
40_I70_N1.0750.79
84_E117_K1.0750.79
455_M565_L1.0750.79
230_T287_D1.0730.79
649_L652_L1.0720.79
34_M78_I1.0720.79
430_P480_E1.0710.79
179_K216_S1.0700.79
561_L564_L1.0700.79
37_E45_L1.0690.79
419_I428_P1.0680.79
15_S18_E1.0680.79
314_T424_F1.0670.79
459_Q520_F1.0650.78
631_T654_Y1.0630.78
80_D584_R1.0630.78
201_M484_R1.0620.78
443_V505_D1.0610.78
378_L385_S1.0610.78
3_S7_D1.0600.78
462_V477_Y1.0590.78
127_S134_R1.0550.77
396_W399_V1.0550.77
417_R421_F1.0530.77
80_D543_R1.0520.77
416_V428_P1.0520.77
1_M4_A1.0510.77
434_C442_P1.0500.77
37_E134_R1.0500.77
235_Y384_G1.0480.77
313_T427_L1.0460.77
417_R420_M1.0450.77
310_V313_T1.0450.77
449_R453_I1.0450.77
506_V509_E1.0440.76
490_I502_K1.0430.76
446_R491_L1.0420.76
281_Y307_G1.0420.76
292_G295_R1.0410.76
211_A228_W1.0410.76
276_L281_Y1.0390.76
81_I85_F1.0380.76
6_V13_I1.0370.76
10_I14_L1.0370.76
241_F269_V1.0360.76
163_S167_F1.0360.76
183_I186_L1.0340.75
319_V424_F1.0340.75
248_F275_Y1.0340.75
79_E562_H1.0330.75
382_S486_M1.0330.75
238_E242_R1.0320.75
469_K687_Q1.0320.75
383_L387_M1.0320.75
20_E24_L1.0310.75
205_G208_T1.0310.75
624_H648_K1.0300.75
281_Y304_G1.0290.75
900_R905_H1.0290.75
20_E26_G1.0280.75
417_R423_S1.0280.75
890_E893_R1.0270.75
362_L384_G1.0250.75
72_R518_E1.0230.74
132_M137_H1.0230.74
30_E111_R1.0220.74
312_M315_R1.0210.74
198_V203_G1.0190.74
4_A9_G1.0180.74
315_R427_L1.0170.74
41_M284_V1.0170.74
38_L385_S1.0160.74
799_I808_L1.0160.74
350_F358_R1.0140.73
182_L826_Q1.0120.73
373_C379_A1.0100.73
232_S444_N1.0100.73
202_G423_S1.0100.73
382_S385_S1.0100.73
705_M708_L1.0090.73
260_L263_L1.0080.73
33_K37_E1.0080.73
710_R783_Y1.0080.73
14_L18_E1.0070.73
623_F626_L1.0060.73
629_L634_T1.0060.73
201_M565_L1.0050.72
441_F444_N1.0040.72
335_L376_L1.0020.72
28_H32_D1.0020.72
79_E83_D1.0010.72
646_M655_L1.0010.72
441_F452_L1.0000.72
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4kxfK80.77651000.699Contact Map
2a5yB20.50321000.758Contact Map
1vt4I80.5681000.766Contact Map
3sfzA10.65771000.767Contact Map
1z6tA40.59721000.844Contact Map
4u09A201000.857Contact Map
4u06A101000.858Contact Map
4u08A201000.858Contact Map
4mn8A10.42661000.864Contact Map
1o6vA20.41041000.865Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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