| btGlut5 |
| ID: | 1468251269 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 501 (471) | ||
| Sequences: | 13780 (7843.3) | ||
| Seq/Len: | 29.257 | ||
| Nf(neff/√len): | 361.4 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 29.257).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 226_D | 229_A | 6.652 | 1.00 |
| 126_E | 130_V | 4.731 | 1.00 |
| 231_K | 244_D | 4.442 | 1.00 |
| 227_E | 251_L | 4.397 | 1.00 |
| 351_V | 386_G | 3.956 | 1.00 |
| 114_L | 118_F | 3.639 | 1.00 |
| 353_F | 450_L | 3.593 | 1.00 |
| 350_S | 453_T | 3.368 | 1.00 |
| 123_K | 127_M | 2.809 | 1.00 |
| 346_L | 453_T | 2.725 | 1.00 |
| 348_G | 390_G | 2.723 | 1.00 |
| 328_I | 385_I | 2.705 | 1.00 |
| 115_L | 131_A | 2.574 | 1.00 |
| 403_F | 411_A | 2.566 | 1.00 |
| 357_C | 446_A | 2.547 | 1.00 |
| 227_E | 231_K | 2.524 | 1.00 |
| 231_K | 247_I | 2.328 | 1.00 |
| 360_T | 443_V | 2.304 | 1.00 |
| 374_Y | 378_A | 2.269 | 1.00 |
| 190_E | 194_I | 2.251 | 1.00 |
| 92_L | 101_T | 2.237 | 1.00 |
| 287_G | 452_T | 2.218 | 1.00 |
| 124_S | 127_M | 2.217 | 1.00 |
| 105_N | 141_L | 2.149 | 1.00 |
| 429_V | 445_F | 2.145 | 1.00 |
| 286_A | 290_L | 2.140 | 1.00 |
| 346_L | 457_F | 2.092 | 1.00 |
| 466_K | 470_E | 2.063 | 1.00 |
| 353_F | 453_T | 2.062 | 1.00 |
| 266_L | 270_K | 2.043 | 1.00 |
| 179_I | 319_A | 2.039 | 1.00 |
| 83_F | 417_T | 2.038 | 1.00 |
| 175_L | 323_A | 2.009 | 1.00 |
| 103_L | 210_L | 2.009 | 1.00 |
| 443_V | 447_V | 1.980 | 1.00 |
| 160_G | 337_E | 1.972 | 1.00 |
| 330_V | 334_F | 1.955 | 1.00 |
| 342_R | 457_F | 1.920 | 1.00 |
| 433_I | 444_I | 1.909 | 1.00 |
| 357_C | 361_G | 1.889 | 1.00 |
| 349_F | 453_T | 1.887 | 1.00 |
| 425_T | 429_V | 1.880 | 1.00 |
| 118_F | 127_M | 1.879 | 1.00 |
| 335_V | 339_M | 1.878 | 1.00 |
| 231_K | 235_R | 1.868 | 1.00 |
| 267_K | 270_K | 1.833 | 1.00 |
| 283_V | 455_Y | 1.824 | 1.00 |
| 238_R | 246_E | 1.823 | 1.00 |
| 282_I | 414_V | 1.819 | 1.00 |
| 94_N | 409_P | 1.810 | 1.00 |
| 103_L | 207_L | 1.804 | 1.00 |
| 117_G | 197_G | 1.797 | 1.00 |
| 337_E | 340_G | 1.789 | 1.00 |
| 455_Y | 459_I | 1.782 | 1.00 |
| 286_A | 422_S | 1.782 | 1.00 |
| 100_G | 103_L | 1.763 | 1.00 |
| 96_L | 100_G | 1.761 | 1.00 |
| 81_G | 133_V | 1.756 | 1.00 |
| 467_T | 470_E | 1.743 | 1.00 |
| 275_R | 279_I | 1.738 | 1.00 |
| 251_L | 255_R | 1.732 | 1.00 |
| 341_R | 401_E | 1.726 | 1.00 |
| 194_I | 198_L | 1.722 | 1.00 |
| 30_F | 176_V | 1.719 | 1.00 |
| 115_L | 127_M | 1.714 | 1.00 |
| 290_L | 422_S | 1.690 | 1.00 |
| 99_K | 103_L | 1.682 | 1.00 |
| 16_V | 236_R | 1.673 | 1.00 |
| 248_E | 252_E | 1.667 | 1.00 |
| 319_A | 323_A | 1.662 | 1.00 |
| 355_A | 382_S | 1.651 | 1.00 |
| 282_I | 418_V | 1.649 | 1.00 |
| 398_L | 460_I | 1.643 | 1.00 |
| 342_R | 346_L | 1.639 | 1.00 |
| 450_L | 454_V | 1.615 | 1.00 |
| 93_V | 145_V | 1.614 | 1.00 |
| 178_Q | 322_G | 1.613 | 1.00 |
| 349_F | 391_P | 1.601 | 1.00 |
| 355_A | 379_C | 1.598 | 1.00 |
| 154_A | 162_L | 1.590 | 1.00 |
| 466_K | 474_I | 1.589 | 1.00 |
| 433_I | 437_L | 1.578 | 1.00 |
| 106_N | 207_L | 1.566 | 1.00 |
| 267_K | 406_S | 1.565 | 1.00 |
| 456_I | 460_I | 1.553 | 1.00 |
| 94_N | 98_R | 1.546 | 1.00 |
| 378_A | 382_S | 1.528 | 1.00 |
| 220_L | 233_A | 1.518 | 1.00 |
| 112_P | 131_A | 1.511 | 1.00 |
| 433_I | 441_S | 1.510 | 1.00 |
| 437_L | 444_I | 1.500 | 1.00 |
| 340_G | 464_K | 1.495 | 1.00 |
| 247_I | 251_L | 1.482 | 1.00 |
| 248_E | 251_L | 1.471 | 1.00 |
| 244_D | 248_E | 1.460 | 1.00 |
| 340_G | 343_F | 1.455 | 1.00 |
| 361_G | 365_L | 1.445 | 1.00 |
| 234_L | 250_I | 1.441 | 1.00 |
| 281_I | 403_F | 1.439 | 1.00 |
| 283_V | 287_G | 1.435 | 1.00 |
| 77_M | 133_V | 1.434 | 1.00 |
| 232_S | 236_R | 1.433 | 1.00 |
| 92_L | 96_L | 1.432 | 1.00 |
| 353_F | 357_C | 1.418 | 1.00 |
| 103_L | 211_P | 1.415 | 1.00 |
| 171_T | 325_N | 1.409 | 1.00 |
| 291_S | 449_C | 1.395 | 0.99 |
| 398_L | 402_I | 1.391 | 0.99 |
| 110_I | 207_L | 1.391 | 0.99 |
| 23_I | 209_F | 1.390 | 0.99 |
| 94_N | 410_A | 1.388 | 0.99 |
| 116_M | 131_A | 1.386 | 0.99 |
| 343_F | 347_L | 1.379 | 0.99 |
| 232_S | 235_R | 1.371 | 0.99 |
| 143_S | 420_W | 1.363 | 0.99 |
| 125_F | 129_I | 1.359 | 0.99 |
| 190_E | 197_G | 1.359 | 0.99 |
| 229_A | 232_S | 1.355 | 0.99 |
| 346_L | 456_I | 1.341 | 0.99 |
| 228_A | 232_S | 1.340 | 0.99 |
| 155_P | 158_W | 1.338 | 0.99 |
| 300_A | 304_Y | 1.335 | 0.99 |
| 89_V | 141_L | 1.330 | 0.99 |
| 346_L | 350_S | 1.327 | 0.99 |
| 429_V | 432_F | 1.325 | 0.99 |
| 454_V | 458_L | 1.321 | 0.99 |
| 454_V | 457_F | 1.314 | 0.99 |
| 283_V | 460_I | 1.313 | 0.99 |
| 424_F | 428_L | 1.303 | 0.99 |
| 81_G | 85_G | 1.296 | 0.99 |
| 353_F | 449_C | 1.294 | 0.99 |
| 336_V | 394_I | 1.284 | 0.99 |
| 218_R | 469_I | 1.284 | 0.99 |
| 339_M | 347_L | 1.281 | 0.99 |
| 375_V | 379_C | 1.281 | 0.99 |
| 98_R | 152_E | 1.274 | 0.99 |
| 265_V | 410_A | 1.268 | 0.99 |
| 347_L | 351_V | 1.267 | 0.99 |
| 208_L | 212_F | 1.265 | 0.99 |
| 91_P | 95_N | 1.265 | 0.99 |
| 130_V | 134_L | 1.256 | 0.99 |
| 255_R | 258_K | 1.254 | 0.99 |
| 387_H | 392_S | 1.247 | 0.99 |
| 229_A | 233_A | 1.245 | 0.98 |
| 444_I | 448_I | 1.237 | 0.98 |
| 77_M | 129_I | 1.231 | 0.98 |
| 235_R | 241_H | 1.228 | 0.98 |
| 451_L | 455_Y | 1.225 | 0.98 |
| 281_I | 415_A | 1.220 | 0.98 |
| 221_L | 227_E | 1.217 | 0.98 |
| 227_E | 247_I | 1.216 | 0.98 |
| 168_L | 326_V | 1.204 | 0.98 |
| 150_L | 162_L | 1.203 | 0.98 |
| 216_S | 219_Y | 1.199 | 0.98 |
| 110_I | 203_A | 1.187 | 0.98 |
| 228_A | 231_K | 1.185 | 0.98 |
| 350_S | 353_F | 1.180 | 0.98 |
| 220_L | 226_D | 1.177 | 0.98 |
| 112_P | 134_L | 1.176 | 0.98 |
| 298_Y | 423_N | 1.174 | 0.98 |
| 189_E | 193_P | 1.163 | 0.97 |
| 218_R | 468_F | 1.163 | 0.97 |
| 439_A | 443_V | 1.162 | 0.97 |
| 98_R | 215_E | 1.161 | 0.97 |
| 279_I | 283_V | 1.159 | 0.97 |
| 172_I | 326_V | 1.158 | 0.97 |
| 117_G | 200_G | 1.155 | 0.97 |
| 252_E | 255_R | 1.155 | 0.97 |
| 230_A | 247_I | 1.154 | 0.97 |
| 110_I | 200_G | 1.153 | 0.97 |
| 421_L | 425_T | 1.152 | 0.97 |
| 119_S | 127_M | 1.151 | 0.97 |
| 453_T | 457_F | 1.147 | 0.97 |
| 287_G | 349_F | 1.137 | 0.97 |
| 349_F | 452_T | 1.135 | 0.97 |
| 234_L | 238_R | 1.135 | 0.97 |
| 466_K | 471_I | 1.133 | 0.97 |
| 220_L | 229_A | 1.130 | 0.97 |
| 404_L | 407_S | 1.127 | 0.97 |
| 358_V | 379_C | 1.124 | 0.97 |
| 264_S | 267_K | 1.123 | 0.97 |
| 350_S | 354_T | 1.114 | 0.96 |
| 252_E | 256_A | 1.109 | 0.96 |
| 108_F | 138_C | 1.108 | 0.96 |
| 80_F | 424_F | 1.108 | 0.96 |
| 189_E | 192_W | 1.106 | 0.96 |
| 358_V | 362_A | 1.099 | 0.96 |
| 325_N | 388_A | 1.099 | 0.96 |
| 219_Y | 226_D | 1.097 | 0.96 |
| 96_L | 104_F | 1.095 | 0.96 |
| 253_E | 257_E | 1.093 | 0.96 |
| 82_G | 420_W | 1.092 | 0.96 |
| 302_Q | 305_L | 1.092 | 0.96 |
| 17_I | 21_T | 1.088 | 0.96 |
| 277_Q | 280_S | 1.083 | 0.96 |
| 291_S | 452_T | 1.080 | 0.95 |
| 127_M | 130_V | 1.071 | 0.95 |
| 235_R | 243_V | 1.070 | 0.95 |
| 175_L | 319_A | 1.069 | 0.95 |
| 410_A | 413_M | 1.067 | 0.95 |
| 85_G | 137_I | 1.064 | 0.95 |
| 347_L | 350_S | 1.062 | 0.95 |
| 148_M | 412_Y | 1.062 | 0.95 |
| 113_A | 200_G | 1.061 | 0.95 |
| 175_L | 326_V | 1.059 | 0.95 |
| 355_A | 386_G | 1.058 | 0.95 |
| 396_A | 416_G | 1.058 | 0.95 |
| 156_K | 467_T | 1.056 | 0.95 |
| 164_V | 397_L | 1.052 | 0.95 |
| 446_A | 450_L | 1.051 | 0.94 |
| 97_G | 215_E | 1.048 | 0.94 |
| 119_S | 122_A | 1.044 | 0.94 |
| 268_L | 411_A | 1.044 | 0.94 |
| 221_L | 230_A | 1.043 | 0.94 |
| 314_V | 318_T | 1.040 | 0.94 |
| 311_E | 314_V | 1.036 | 0.94 |
| 114_L | 200_G | 1.036 | 0.94 |
| 160_G | 341_R | 1.036 | 0.94 |
| 324_V | 328_I | 1.030 | 0.94 |
| 351_V | 355_A | 1.029 | 0.94 |
| 347_L | 386_G | 1.027 | 0.94 |
| 171_T | 329_T | 1.022 | 0.93 |
| 339_M | 344_L | 1.013 | 0.93 |
| 407_S | 411_A | 1.011 | 0.93 |
| 215_E | 219_Y | 1.009 | 0.93 |
| 263_I | 267_K | 1.009 | 0.93 |
| 288_Q | 419_H | 1.007 | 0.93 |
| 356_C | 360_T | 1.003 | 0.93 |
| 112_P | 135_V | 1.002 | 0.92 |
| 285_M | 395_P | 1.001 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4pypA | 1 | 0.8922 | 100 | 0.42 | Contact Map |
| 4zw9A | 1 | 0 | 100 | 0.429 | Contact Map |
| 4ldsA | 2 | 0.8383 | 100 | 0.443 | Contact Map |
| 4gc0A | 1 | 0.8922 | 100 | 0.444 | Contact Map |
| 4j05A | 2 | 0.7745 | 100 | 0.49 | Contact Map |
| 1pw4A | 1 | 0.8303 | 100 | 0.596 | Contact Map |
| 3wdoA | 1 | 0.8463 | 100 | 0.598 | Contact Map |
| 3o7qA | 1 | 0.7685 | 100 | 0.634 | Contact Map |
| 2gfpA | 2 | 0.7445 | 100 | 0.643 | Contact Map |
| 4xnjA | 1 | 0 | 100 | 0.647 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)