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OPENSEQ.org
SCS1-2-3 -force run
ID: 1455807288 Query Sequence

 Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 970 (905)
Sequences: 57 (29.5)
Seq/Len: 0.063
Nf(neff/√len): 1.0
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 0.063).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.
Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:
GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
81_S85_G1.9490.29
75_E78_L1.6800.20
414_S457_H1.6740.20
885_G902_T1.6470.19
308_N757_V1.6090.18
88_Y111_K1.6040.18
471_M497_T1.6000.18
170_S179_L1.5690.17
193_V201_D1.5560.17
61_I79_W1.5390.16
197_N204_H1.5360.16
80_R85_G1.5300.16
287_I645_S1.5250.16
754_Y857_N1.5160.16
62_L79_W1.5130.16
64_K73_I1.5100.15
514_T532_V1.5070.15
442_S634_Y1.4990.15
354_K605_Y1.4850.15
108_P111_K1.4770.15
536_Y736_F1.4740.15
136_Y141_L1.4640.14
955_A959_A1.4620.14
658_A834_I1.4520.14
85_G110_N1.4490.14
76_S79_W1.4360.14
313_D685_G1.4360.14
75_E79_W1.4360.14
642_K719_L1.4330.14
167_L170_S1.4300.14
83_D88_Y1.4270.14
167_L174_G1.4270.14
167_L177_W1.4250.14
497_T532_V1.4200.14
62_L76_S1.4150.13
62_L78_L1.4150.13
155_E161_Y1.4150.13
820_G826_V1.4150.13
62_L75_E1.4140.13
105_Y111_K1.4140.13
129_S143_F1.4080.13
81_S110_N1.4050.13
197_N206_E1.4050.13
81_S84_Y1.4020.13
605_Y824_I1.4000.13
125_S138_K1.3990.13
256_G605_Y1.3970.13
275_L830_A1.3910.13
172_E176_R1.3900.13
80_R110_N1.3900.13
61_I107_C1.3810.13
129_S151_H1.3780.13
88_Y108_P1.3780.13
275_L771_V1.3720.13
319_F536_Y1.3530.12
170_S174_G1.3520.12
87_T101_L1.3500.12
497_T514_T1.3460.12
884_T899_L1.3430.12
174_G184_V1.3410.12
804_P807_T1.3370.12
134_A145_I1.3310.12
134_A138_K1.3310.12
328_S519_V1.3310.12
174_G177_W1.3310.12
170_S177_W1.3290.12
333_E471_M1.3250.12
170_S184_V1.3250.12
79_W85_G1.3250.12
85_G88_Y1.3230.12
64_K78_L1.3220.12
206_E244_V1.3200.12
64_K76_S1.3160.11
193_V203_V1.3160.11
64_K75_E1.3150.11
69_N145_I1.3100.11
125_S134_A1.3040.11
328_S567_R1.3030.11
442_S870_R1.3030.11
326_V517_M1.3010.11
295_F705_Q1.3010.11
61_I109_T1.2910.11
275_L835_L1.2880.11
261_Y737_R1.2840.11
956_H959_A1.2820.11
81_S88_Y1.2780.11
602_D639_G1.2780.11
902_T938_N1.2760.11
90_K111_K1.2730.11
197_N244_V1.2720.11
168_Y179_L1.2720.11
295_F572_R1.2700.11
155_E160_A1.2690.11
414_S798_Y1.2670.11
73_I78_L1.2660.11
414_S676_A1.2570.10
897_P900_P1.2570.10
223_N884_T1.2570.10
642_K652_V1.2540.10
73_I76_S1.2520.10
88_Y110_N1.2510.10
273_M332_P1.2510.10
643_V652_V1.2490.10
907_V919_R1.2490.10
255_S417_T1.2480.10
604_G628_C1.2480.10
937_Q962_L1.2470.10
86_T90_K1.2470.10
556_S571_K1.2470.10
249_V635_L1.2390.10
83_D111_K1.2380.10
167_L179_L1.2360.10
103_Y106_V1.2360.10
240_D582_K1.2330.10
319_F945_K1.2330.10
525_H688_V1.2270.10
356_I732_N1.2270.10
567_R706_V1.2240.10
782_A880_L1.2200.10
65_L70_L1.2200.10
328_S497_T1.2180.10
131_D150_F1.2180.10
251_V955_A1.2170.10
295_F719_L1.2140.10
259_P795_V1.2130.10
68_Y74_T1.2110.10
254_T451_Q1.2100.10
272_Q784_L1.2070.10
73_I79_W1.2050.10
806_I828_V1.2040.10
511_G757_V1.2040.10
472_K634_Y1.2040.10
511_G664_P1.2020.10
308_N511_G1.2010.10
658_A876_I1.1990.10
780_A824_I1.1990.10
757_V778_V1.1960.09
93_D106_V1.1930.09
179_L184_V1.1920.09
308_N342_E1.1890.09
748_S785_W1.1830.09
938_N957_L1.1830.09
96_G111_K1.1830.09
520_F529_W1.1810.09
85_G111_K1.1770.09
83_D110_N1.1770.09
427_N905_L1.1770.09
92_N105_Y1.1760.09
177_W206_E1.1740.09
699_V717_V1.1740.09
531_L554_L1.1740.09
64_K79_W1.1730.09
88_Y105_Y1.1730.09
689_T865_I1.1710.09
379_D732_N1.1710.09
71_G113_K1.1700.09
143_F151_H1.1700.09
131_D151_H1.1680.09
471_M514_T1.1680.09
332_P902_T1.1680.09
808_L836_Q1.1680.09
126_L573_K1.1670.09
284_M311_I1.1620.09
448_T784_L1.1610.09
137_Q156_D1.1610.09
131_D143_F1.1610.09
448_T739_T1.1590.09
83_D105_Y1.1580.09
105_Y108_P1.1560.09
886_V899_L1.1560.09
897_P901_T1.1560.09
403_S440_F1.1550.09
326_V775_N1.1520.09
262_Y370_R1.1520.09
61_I66_Y1.1500.09
351_L473_H1.1490.09
483_L508_G1.1470.09
326_V469_V1.1470.09
174_G179_L1.1460.09
638_T689_T1.1450.09
820_G843_V1.1450.09
399_H510_P1.1430.09
143_F160_A1.1400.09
371_D950_V1.1370.09
347_K950_V1.1370.09
304_N961_P1.1370.09
774_K881_V1.1360.09
800_N835_L1.1360.09
414_S478_I1.1360.09
256_G899_L1.1340.09
326_V778_V1.1340.09
788_Q830_A1.1340.09
81_S111_K1.1330.09
107_C110_N1.1330.09
292_N471_M1.1330.09
328_S474_T1.1330.09
611_G694_L1.1310.09
261_Y298_V1.1300.09
350_F399_H1.1300.09
287_I752_V1.1280.09
151_H161_Y1.1270.08
287_I478_I1.1270.08
88_Y109_T1.1270.08
100_I448_T1.1250.08
84_Y92_N1.1250.08
547_E569_Y1.1240.08
471_M532_V1.1240.08
266_R792_L1.1220.08
399_H471_M1.1220.08
129_S160_A1.1220.08
638_T680_L1.1190.08
662_K872_I1.1190.08
262_Y652_V1.1190.08
414_S724_D1.1190.08
667_A673_I1.1190.08
275_L287_I1.1190.08
563_M646_N1.1180.08
739_T925_E1.1180.08
808_L820_G1.1160.08
204_H244_V1.1160.08
892_L906_F1.1160.08
272_Q475_S1.1150.08
634_Y887_P1.1150.08
96_G100_I1.1130.08
858_L868_W1.1130.08
754_Y829_S1.1130.08
138_K275_L1.1130.08
203_V206_E1.1100.08
68_Y73_I1.1100.08
80_R84_Y1.1100.08
522_H531_L1.1100.08
643_V737_R1.1100.08
418_A469_V1.1090.08
567_R570_K1.1090.08
572_R634_Y1.1080.08
366_Y371_D1.1080.08
76_S83_D1.1060.08
891_I895_V1.1060.08
105_Y113_K1.1050.08
583_Y920_S1.1050.08
66_Y79_W1.1050.08
156_D757_V1.1050.08
225_T824_I1.1050.08
240_D441_V1.1050.08
333_E497_T1.1050.08
351_L416_D1.1050.08
79_W84_Y1.1030.08
529_W533_K1.1030.08
739_T784_L1.1030.08
105_Y110_N1.1020.08
90_K110_N1.1020.08
290_D448_T1.1000.08
353_N369_G1.1000.08
353_N372_W1.1000.08
353_N375_L1.1000.08
353_N796_W1.1000.08
369_G372_W1.1000.08
369_G375_L1.1000.08
369_G796_W1.1000.08
372_W375_L1.1000.08
372_W796_W1.1000.08
375_L796_W1.1000.08
77_S80_R1.1000.08
535_D548_D1.1000.08
412_I424_A1.1000.08
311_I792_L1.0990.08
457_H722_M1.0990.08
151_H160_A1.0990.08
203_V254_T1.0990.08
80_R88_Y1.0970.08
61_I81_S1.0970.08
440_F501_L1.0970.08
469_V517_M1.0970.08
571_K643_V1.0970.08
327_Q894_A1.0960.08
921_T956_H1.0960.08
64_K77_S1.0940.08
85_G101_L1.0940.08
75_E83_D1.0930.08
62_L73_I1.0930.08
147_S157_W1.0910.08
589_S872_I1.0900.08
102_S106_V1.0880.08
135_T948_V1.0880.08
371_D778_V1.0880.08
397_H503_V1.0880.08
308_N778_V1.0880.08
418_A778_V1.0870.08
134_A155_E1.0850.08
217_L667_A1.0850.08
677_D736_F1.0840.08
87_T108_P1.0820.08
67_D74_T1.0820.08
730_Y738_V1.0810.08
464_Q954_A1.0790.08
556_S672_R1.0790.08
389_C820_G1.0790.08
778_V799_G1.0780.08
815_R833_A1.0780.08
126_L515_L1.0760.08
782_A908_L1.0760.08
891_I900_P1.0760.08
216_Y324_E1.0760.08
157_W164_D1.0760.08
73_I77_S1.0750.08
83_D90_K1.0750.08
78_L83_D1.0750.08
80_R83_D1.0750.08
319_F736_F1.0750.08
85_G90_K1.0740.08
279_A472_K1.0740.08
536_Y925_E1.0740.08
223_N332_P1.0720.08
105_Y109_T1.0720.08
931_L950_V1.0720.08
275_L935_L1.0720.08
101_L106_V1.0710.08
328_S514_T1.0710.08
669_R812_I1.0690.08
223_N899_L1.0690.08
308_N333_E1.0680.08
589_S949_R1.0680.08
295_F865_I1.0660.08
265_Y368_K1.0660.08
73_I80_R1.0660.08
61_I85_G1.0660.08
85_G105_Y1.0660.08
212_G781_T1.0650.08
117_L157_W1.0650.08
151_H156_D1.0650.08
153_K351_L1.0630.08
367_N827_Q1.0630.08
81_S101_L1.0620.08
238_D908_L1.0620.08
255_S545_A1.0600.08
326_V342_E1.0600.08
335_N689_T1.0600.08
94_K101_L1.0600.08
447_N800_N1.0590.08
515_L532_V1.0590.08
85_G113_K1.0590.08
117_L147_S1.0590.08
569_Y582_K1.0590.08
102_S113_K1.0590.08
88_Y96_G1.0570.08
923_D927_I1.0570.08
64_K74_T1.0570.08
62_L81_S1.0570.08
87_T111_K1.0570.08
117_L123_E1.0570.08
857_N870_R1.0540.08
688_V881_V1.0540.08
502_F581_G1.0540.08
399_H497_T1.0540.08
124_S130_S1.0530.08
570_K588_E1.0530.08
634_Y905_L1.0530.08
667_A688_V1.0510.08
315_R330_R1.0510.08
467_V740_V1.0510.08
764_V925_E1.0500.07
595_T721_S1.0500.07
508_G515_L1.0500.07
554_L563_M1.0480.07
284_M919_R1.0480.07
527_S531_L1.0480.07
254_T365_T1.0480.07
843_V895_V1.0480.07
175_R776_K1.0470.07
333_E399_H1.0450.07
286_V556_S1.0450.07
830_A835_L1.0450.07
402_V483_L1.0450.07
602_D628_C1.0450.07
604_G639_G1.0450.07
628_C639_G1.0450.07
240_D689_T1.0450.07
151_H191_W1.0440.07
61_I572_R1.0420.07
129_S155_E1.0410.07
667_A889_Q1.0410.07
167_L184_V1.0410.07
408_T724_D1.0410.07
574_S600_D1.0410.07
374_L556_S1.0410.07
824_I837_D1.0400.07
88_Y92_N1.0400.07
597_A721_S1.0400.07
310_Y316_G1.0400.07
312_S316_G1.0400.07
70_L142_N1.0400.07
275_L284_M1.0400.07
293_Q749_D1.0400.07
418_A513_E1.0400.07
117_L127_L1.0400.07
924_L961_P1.0380.07
937_Q961_P1.0380.07
93_D102_S1.0380.07
329_S783_V1.0370.07
804_P826_V1.0370.07
279_A442_S1.0350.07
829_S840_T1.0350.07
123_E149_L1.0350.07
351_L899_L1.0350.07
517_M775_N1.0350.07
61_I76_S1.0340.07
775_N778_V1.0340.07
91_L154_Q1.0340.07
804_P820_G1.0340.07
519_V596_E1.0340.07
669_R774_K1.0320.07
61_I75_E1.0320.07
61_I78_L1.0320.07
553_Q844_Y1.0320.07
117_L124_S1.0310.07
90_K108_P1.0310.07
904_E907_V1.0290.07
595_T848_R1.0290.07
81_S90_K1.0290.07
92_N96_G1.0280.07
80_R111_K1.0280.07
77_S90_K1.0280.07
136_Y759_C1.0280.07
525_H727_K1.0280.07
131_D152_P1.0280.07
130_S142_N1.0260.07
162_S189_F1.0260.07
626_K691_M1.0260.07
141_L192_S1.0260.07
542_R643_V1.0260.07
810_G928_S1.0260.07
397_H450_R1.0250.07
310_Y339_D1.0250.07
312_S339_D1.0250.07
316_G339_D1.0250.07
66_Y77_S1.0250.07
62_L85_G1.0230.07
328_S471_M1.0230.07
367_N646_N1.0230.07
471_M645_S1.0230.07
81_S105_Y1.0220.07
125_S275_L1.0220.07
275_L298_V1.0220.07
527_S554_L1.0220.07
346_I776_K1.0220.07
680_L809_E1.0220.07
667_A872_I1.0200.07
469_V778_V1.0200.07
399_H474_T1.0200.07
106_V111_K1.0190.07
328_S884_T1.0190.07
589_S667_A1.0190.07
318_Y325_N1.0170.07
895_V900_P1.0170.07
365_T371_D1.0160.07
126_L706_V1.0160.07
606_E631_G1.0160.07
839_K928_S1.0160.07
177_W197_N1.0140.07
850_L865_I1.0140.07
180_I475_S1.0130.07
343_V353_N1.0130.07
343_V369_G1.0130.07
343_V372_W1.0130.07
343_V375_L1.0130.07
343_V796_W1.0130.07
481_L502_F1.0130.07
519_V768_L1.0130.07
274_K727_K1.0130.07
67_D78_L1.0130.07
483_L512_E1.0130.07
65_L93_D1.0110.07
284_M569_Y1.0110.07
417_T559_E1.0110.07
150_F161_Y1.0100.07
124_S157_W1.0100.07
808_L837_D1.0100.07
61_I80_R1.0100.07
376_Q857_N1.0100.07
232_P274_K1.0080.07
393_Y411_I1.0080.07
166_K287_I1.0080.07
67_D75_E1.0080.07
812_I865_I1.0080.07
862_N869_R1.0070.07
843_V901_T1.0070.07
141_L308_N1.0070.07
478_I645_S1.0060.07
138_K145_I1.0040.07
562_I921_T1.0040.07
167_L206_E1.0040.07
67_D76_S1.0030.07
98_K101_L1.0030.07
123_E147_S1.0030.07
351_L886_V1.0030.07
460_L515_L1.0030.07
773_T795_V1.0030.07
627_D768_L1.0030.07
285_H302_N1.0010.07
425_S483_L1.0000.07
519_V567_R1.0000.07
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features 		HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3wsxA10.58871000.744Contact Map
3f6kA10.64541000.766Contact Map
1wgoA10.12681000.91Contact Map
2cn3A20.609399.90.953Contact Map
4lgnA10.699.70.965Contact Map
1sqjA20.557799.50.969Contact Map
3a0fA10.560899.20.974Contact Map
2c4xA10.244399.10.975Contact Map
1b4rA10.0825990.976Contact Map
2xbgA10.30198.80.977Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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