| Q8IUS5 |
| ID: | 1446753312 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 325 (290) | ||
| Sequences: | 18553 (12649.8) | ||
| Seq/Len: | 63.976 | ||
| Nf(neff/√len): | 742.8 |
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 63.976).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 127_V | 151_K | 5.111 | 1.00 |
| 91_R | 113_D | 5.094 | 1.00 |
| 50_A | 84_R | 4.902 | 1.00 |
| 268_E | 295_S | 3.747 | 1.00 |
| 263_L | 316_F | 3.234 | 1.00 |
| 125_K | 150_M | 3.102 | 1.00 |
| 75_Q | 310_N | 3.082 | 1.00 |
| 292_T | 312_L | 2.872 | 1.00 |
| 115_K | 141_I | 2.755 | 1.00 |
| 135_G | 157_F | 2.687 | 1.00 |
| 305_Q | 308_I | 2.593 | 1.00 |
| 119_D | 148_M | 2.575 | 1.00 |
| 128_L | 138_A | 2.550 | 1.00 |
| 142_A | 152_L | 2.538 | 1.00 |
| 118_L | 126_C | 2.487 | 1.00 |
| 156_N | 270_D | 2.480 | 1.00 |
| 127_V | 150_M | 2.360 | 1.00 |
| 290_R | 319_E | 2.359 | 1.00 |
| 155_I | 309_V | 2.336 | 1.00 |
| 45_R | 97_D | 2.320 | 1.00 |
| 112_T | 144_C | 2.308 | 1.00 |
| 294_L | 312_L | 2.187 | 1.00 |
| 268_E | 293_I | 2.175 | 1.00 |
| 48_Y | 117_I | 2.152 | 1.00 |
| 115_K | 145_Y | 2.135 | 1.00 |
| 127_V | 153_I | 2.096 | 1.00 |
| 89_D | 94_G | 2.001 | 1.00 |
| 62_L | 114_I | 1.991 | 1.00 |
| 275_V | 293_I | 1.984 | 1.00 |
| 58_L | 86_V | 1.980 | 1.00 |
| 115_K | 119_D | 1.935 | 1.00 |
| 153_I | 313_I | 1.929 | 1.00 |
| 88_L | 117_I | 1.916 | 1.00 |
| 141_I | 145_Y | 1.910 | 1.00 |
| 138_A | 152_L | 1.896 | 1.00 |
| 282_K | 289_F | 1.893 | 1.00 |
| 47_H | 95_E | 1.877 | 1.00 |
| 49_V | 69_W | 1.873 | 1.00 |
| 82_E | 314_W | 1.872 | 1.00 |
| 114_I | 141_I | 1.859 | 1.00 |
| 46_F | 96_T | 1.853 | 1.00 |
| 36_Y | 45_R | 1.848 | 1.00 |
| 62_L | 138_A | 1.827 | 1.00 |
| 127_V | 317_L | 1.822 | 1.00 |
| 88_L | 114_I | 1.794 | 1.00 |
| 108_D | 144_C | 1.789 | 1.00 |
| 312_L | 315_T | 1.780 | 1.00 |
| 311_K | 315_T | 1.768 | 1.00 |
| 60_L | 118_L | 1.761 | 1.00 |
| 38_R | 45_R | 1.748 | 1.00 |
| 63_H | 93_Y | 1.717 | 1.00 |
| 62_L | 90_L | 1.706 | 1.00 |
| 297_A | 305_Q | 1.696 | 1.00 |
| 263_L | 292_T | 1.683 | 1.00 |
| 58_L | 84_R | 1.651 | 1.00 |
| 115_K | 148_M | 1.607 | 1.00 |
| 151_K | 320_E | 1.587 | 1.00 |
| 50_A | 86_V | 1.571 | 1.00 |
| 140_L | 144_C | 1.559 | 1.00 |
| 153_I | 263_L | 1.536 | 1.00 |
| 141_I | 149_V | 1.535 | 1.00 |
| 145_Y | 148_M | 1.528 | 1.00 |
| 152_L | 262_T | 1.526 | 1.00 |
| 64_G | 132_D | 1.523 | 1.00 |
| 132_D | 299_H | 1.522 | 1.00 |
| 35_C | 48_Y | 1.505 | 1.00 |
| 40_K | 116_D | 1.491 | 1.00 |
| 106_K | 109_C | 1.478 | 1.00 |
| 154_V | 157_F | 1.447 | 1.00 |
| 63_H | 90_L | 1.447 | 1.00 |
| 51_A | 87_A | 1.442 | 1.00 |
| 66_P | 132_D | 1.436 | 1.00 |
| 86_V | 117_I | 1.433 | 1.00 |
| 90_L | 137_I | 1.416 | 1.00 |
| 60_L | 114_I | 1.396 | 0.99 |
| 118_L | 123_Y | 1.392 | 0.99 |
| 61_L | 129_I | 1.391 | 0.99 |
| 46_F | 113_D | 1.385 | 0.99 |
| 47_H | 89_D | 1.379 | 0.99 |
| 58_L | 123_Y | 1.368 | 0.99 |
| 61_L | 85_V | 1.356 | 0.99 |
| 76_L | 87_A | 1.349 | 0.99 |
| 308_I | 312_L | 1.330 | 0.99 |
| 47_H | 69_W | 1.312 | 0.99 |
| 78_E | 310_N | 1.311 | 0.99 |
| 118_L | 141_I | 1.297 | 0.99 |
| 279_E | 291_L | 1.287 | 0.99 |
| 52_G | 84_R | 1.282 | 0.99 |
| 139_W | 154_V | 1.279 | 0.99 |
| 69_W | 89_D | 1.260 | 0.99 |
| 308_I | 311_K | 1.253 | 0.99 |
| 40_K | 43_G | 1.238 | 0.98 |
| 62_L | 88_L | 1.231 | 0.98 |
| 268_E | 296_E | 1.231 | 0.98 |
| 265_L | 312_L | 1.222 | 0.98 |
| 151_K | 261_P | 1.220 | 0.98 |
| 128_L | 149_V | 1.220 | 0.98 |
| 279_E | 293_I | 1.201 | 0.98 |
| 111_I | 137_I | 1.201 | 0.98 |
| 254_K | 257_M | 1.194 | 0.98 |
| 83_Y | 318_K | 1.192 | 0.98 |
| 119_D | 145_Y | 1.182 | 0.98 |
| 123_Y | 126_C | 1.178 | 0.98 |
| 90_L | 133_W | 1.168 | 0.97 |
| 83_Y | 127_V | 1.152 | 0.97 |
| 220_T | 223_D | 1.150 | 0.97 |
| 60_L | 126_C | 1.135 | 0.97 |
| 56_K | 84_R | 1.132 | 0.97 |
| 307_D | 311_K | 1.130 | 0.97 |
| 294_L | 297_A | 1.129 | 0.97 |
| 76_L | 85_V | 1.128 | 0.97 |
| 269_N | 296_E | 1.127 | 0.97 |
| 129_I | 313_I | 1.126 | 0.97 |
| 67_E | 71_S | 1.123 | 0.97 |
| 115_K | 144_C | 1.109 | 0.96 |
| 151_K | 316_F | 1.109 | 0.96 |
| 130_G | 135_G | 1.107 | 0.96 |
| 117_I | 121_L | 1.093 | 0.96 |
| 86_V | 121_L | 1.092 | 0.96 |
| 111_I | 140_L | 1.091 | 0.96 |
| 280_V | 284_Y | 1.089 | 0.96 |
| 297_A | 301_L | 1.088 | 0.96 |
| 279_E | 283_I | 1.083 | 0.96 |
| 53_E | 84_R | 1.083 | 0.96 |
| 34_H | 47_H | 1.077 | 0.95 |
| 69_W | 95_E | 1.072 | 0.95 |
| 126_C | 149_V | 1.071 | 0.95 |
| 141_I | 148_M | 1.070 | 0.95 |
| 265_L | 309_V | 1.067 | 0.95 |
| 309_V | 313_I | 1.062 | 0.95 |
| 266_W | 275_V | 1.058 | 0.95 |
| 290_R | 312_L | 1.057 | 0.95 |
| 128_L | 141_I | 1.038 | 0.94 |
| 83_Y | 317_L | 1.034 | 0.94 |
| 290_R | 315_T | 1.029 | 0.94 |
| 297_A | 304_D | 1.022 | 0.93 |
| 74_Y | 77_R | 1.011 | 0.93 |
| 57_P | 83_Y | 1.008 | 0.93 |
| 153_I | 316_F | 1.007 | 0.93 |
| 44_L | 91_R | 1.000 | 0.92 |
| 130_G | 134_G | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4inzA | 2 | 0.8492 | 100 | 0.276 | Contact Map |
| 1ehyA | 4 | 0.8338 | 100 | 0.288 | Contact Map |
| 3oosA | 1 | 0.8277 | 100 | 0.321 | Contact Map |
| 3om8A | 2 | 0.8092 | 100 | 0.331 | Contact Map |
| 4psuA | 1 | 0.8708 | 100 | 0.333 | Contact Map |
| 3r40A | 2 | 0.8646 | 100 | 0.338 | Contact Map |
| 2xuaA | 2 | 0.7908 | 100 | 0.342 | Contact Map |
| 5bovA | 4 | 0 | 100 | 0.343 | Contact Map |
| 2pujA | 1 | 0.8369 | 100 | 0.344 | Contact Map |
| 1iupA | 1 | 0.8062 | 100 | 0.352 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)