| 1149 |
| ID: | 1445009442 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 81 (68) | ||
| Sequences: | 10362 (6687.6) | ||
| Seq/Len: | 152.382 | ||
| Nf(neff/√len): | 811.0 |
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 152.382).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 31_Y | 67_R | 4.733 | 1.00 |
| 18_S | 70_Y | 2.933 | 1.00 |
| 34_L | 54_E | 2.821 | 1.00 |
| 14_E | 74_L | 2.284 | 1.00 |
| 38_K | 50_E | 2.185 | 1.00 |
| 29_D | 36_E | 1.891 | 1.00 |
| 22_R | 28_A | 1.872 | 1.00 |
| 69_D | 72_E | 1.733 | 1.00 |
| 18_S | 22_R | 1.664 | 1.00 |
| 61_D | 64_N | 1.572 | 1.00 |
| 65_D | 69_D | 1.553 | 1.00 |
| 25_D | 30_G | 1.550 | 1.00 |
| 60_G | 76_F | 1.533 | 1.00 |
| 23_M | 43_A | 1.529 | 1.00 |
| 26_K | 39_I | 1.518 | 1.00 |
| 38_K | 53_I | 1.425 | 1.00 |
| 60_G | 68_I | 1.397 | 0.99 |
| 49_T | 52_D | 1.357 | 0.99 |
| 22_R | 70_Y | 1.317 | 0.99 |
| 34_L | 57_M | 1.296 | 0.99 |
| 70_Y | 74_L | 1.285 | 0.99 |
| 62_K | 75_E | 1.275 | 0.99 |
| 27_N | 36_E | 1.256 | 0.99 |
| 25_D | 36_E | 1.246 | 0.98 |
| 24_F | 40_M | 1.241 | 0.98 |
| 38_K | 42_Q | 1.224 | 0.98 |
| 16_E | 19_D | 1.162 | 0.97 |
| 14_E | 18_S | 1.135 | 0.97 |
| 31_Y | 69_D | 1.132 | 0.97 |
| 29_D | 33_D | 1.126 | 0.97 |
| 65_D | 72_E | 1.124 | 0.97 |
| 48_I | 52_D | 1.083 | 0.96 |
| 61_D | 65_D | 1.059 | 0.95 |
| 33_D | 36_E | 1.045 | 0.94 |
| 54_E | 58_K | 1.038 | 0.94 |
| 26_K | 36_E | 1.032 | 0.94 |
| 50_E | 53_I | 1.026 | 0.94 |
| 21_F | 70_Y | 1.016 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3u0kA | 1 | 0.8519 | 98.8 | 0.129 | Contact Map |
| 2kz2A | 1 | 0.9506 | 98.8 | 0.132 | Contact Map |
| 4d1eA | 1 | 1 | 98.8 | 0.134 | Contact Map |
| 4i2yA | 2 | 0.9136 | 98.8 | 0.136 | Contact Map |
| 4wpxA | 2 | 0 | 98.8 | 0.141 | Contact Map |
| 4ociA | 1 | 0.963 | 98.7 | 0.144 | Contact Map |
| 2mysB | 1 | 0.8765 | 98.7 | 0.146 | Contact Map |
| 3mwuA | 1 | 0.7531 | 98.7 | 0.149 | Contact Map |
| 3j04B | 2 | 0.963 | 98.7 | 0.152 | Contact Map |
| 4ds7A | 4 | 0.9259 | 98.7 | 0.154 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)