| CHEY_P0AW67 |
| ID: | 1444814564 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 206 (177) | ||
| Sequences: | 2805 (2414.5) | ||
| Seq/Len: | 15.847 | ||
| Nf(neff/√len): | 181.5 |
HHblits Results: (2015_06)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 15.847).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 110_V | 128_Y | 3.955 | 1.00 |
| 95_R | 112_E | 3.083 | 1.00 |
| 133_S | 145_L | 2.835 | 1.00 |
| 105_L | 194_E | 2.560 | 1.00 |
| 46_E | 49_H | 2.444 | 1.00 |
| 43_Y | 53_C | 2.411 | 1.00 |
| 141_D | 144_E | 2.253 | 1.00 |
| 42_E | 54_H | 2.251 | 1.00 |
| 114_E | 118_D | 2.201 | 1.00 |
| 146_L | 160_V | 2.188 | 1.00 |
| 148_T | 151_A | 2.178 | 1.00 |
| 45_S | 51_R | 2.137 | 1.00 |
| 88_V | 119_A | 2.091 | 1.00 |
| 115_D | 139_N | 2.010 | 1.00 |
| 113_A | 122_K | 1.988 | 1.00 |
| 120_L | 145_L | 1.960 | 1.00 |
| 99_R | 103_K | 1.951 | 1.00 |
| 84_K | 108_N | 1.937 | 1.00 |
| 80_D | 107_F | 1.933 | 1.00 |
| 87_V | 98_V | 1.883 | 1.00 |
| 150_R | 181_S | 1.877 | 1.00 |
| 84_K | 110_V | 1.852 | 1.00 |
| 86_L | 113_A | 1.800 | 1.00 |
| 95_R | 114_E | 1.759 | 1.00 |
| 45_S | 48_C | 1.742 | 1.00 |
| 117_V | 139_N | 1.738 | 1.00 |
| 116_G | 140_M | 1.736 | 1.00 |
| 162_M | 180_A | 1.716 | 1.00 |
| 182_G | 199_K | 1.702 | 1.00 |
| 130_F | 197_L | 1.652 | 1.00 |
| 85_F | 107_F | 1.639 | 1.00 |
| 135_W | 162_M | 1.577 | 1.00 |
| 24_H | 28_T | 1.568 | 1.00 |
| 116_G | 145_L | 1.548 | 1.00 |
| 124_Q | 152_D | 1.530 | 1.00 |
| 89_D | 95_R | 1.514 | 1.00 |
| 161_L | 196_K | 1.503 | 1.00 |
| 110_V | 122_K | 1.476 | 1.00 |
| 87_V | 109_N | 1.474 | 1.00 |
| 120_L | 140_M | 1.449 | 1.00 |
| 123_L | 149_I | 1.446 | 1.00 |
| 133_S | 146_L | 1.433 | 1.00 |
| 89_D | 137_M | 1.431 | 1.00 |
| 25_E | 29_A | 1.419 | 1.00 |
| 136_N | 166_E | 1.404 | 0.99 |
| 146_L | 180_A | 1.404 | 0.99 |
| 163_V | 188_F | 1.397 | 0.99 |
| 117_V | 121_N | 1.383 | 0.99 |
| 26_M | 30_X | 1.368 | 0.99 |
| 115_D | 118_D | 1.357 | 0.99 |
| 83_L | 107_F | 1.357 | 0.99 |
| 84_K | 128_Y | 1.351 | 0.99 |
| 164_T | 183_Y | 1.347 | 0.99 |
| 99_R | 112_E | 1.324 | 0.99 |
| 84_K | 129_G | 1.294 | 0.99 |
| 82_E | 107_F | 1.265 | 0.99 |
| 144_E | 147_K | 1.254 | 0.99 |
| 189_T | 192_T | 1.250 | 0.99 |
| 85_F | 130_F | 1.248 | 0.99 |
| 134_D | 186_K | 1.247 | 0.99 |
| 131_V | 149_I | 1.241 | 0.98 |
| 120_L | 148_T | 1.235 | 0.98 |
| 88_V | 116_G | 1.234 | 0.98 |
| 90_D | 134_D | 1.219 | 0.98 |
| 80_D | 83_L | 1.216 | 0.98 |
| 45_S | 50_E | 1.214 | 0.98 |
| 144_E | 148_T | 1.190 | 0.98 |
| 90_D | 165_A | 1.188 | 0.98 |
| 83_L | 201_F | 1.185 | 0.98 |
| 182_G | 196_K | 1.182 | 0.98 |
| 25_E | 28_T | 1.169 | 0.97 |
| 131_V | 158_L | 1.168 | 0.97 |
| 44_O | 54_H | 1.153 | 0.97 |
| 83_L | 198_N | 1.141 | 0.97 |
| 48_C | 51_R | 1.131 | 0.97 |
| 101_L | 190_A | 1.123 | 0.97 |
| 146_L | 162_M | 1.117 | 0.96 |
| 92_S | 95_R | 1.108 | 0.96 |
| 98_V | 188_F | 1.083 | 0.96 |
| 90_D | 137_M | 1.082 | 0.96 |
| 123_L | 153_G | 1.080 | 0.95 |
| 105_L | 190_A | 1.067 | 0.95 |
| 22_I | 25_E | 1.065 | 0.95 |
| 132_I | 193_L | 1.061 | 0.95 |
| 90_D | 164_T | 1.059 | 0.95 |
| 50_E | 53_C | 1.054 | 0.95 |
| 148_T | 152_D | 1.053 | 0.95 |
| 26_M | 29_A | 1.041 | 0.94 |
| 135_W | 143_L | 1.039 | 0.94 |
| 116_G | 120_L | 1.035 | 0.94 |
| 41_H | 45_S | 1.028 | 0.94 |
| 44_O | 52_I | 1.026 | 0.94 |
| 45_S | 49_H | 1.020 | 0.93 |
| 132_I | 163_V | 1.017 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3mm4A | 1 | 0.6214 | 100 | 0.429 | Contact Map |
| 3r0jA | 2 | 0.6019 | 99.9 | 0.527 | Contact Map |
| 1w25A | 2 | 0.6019 | 99.9 | 0.533 | Contact Map |
| 3dzdA | 2 | 0.5874 | 99.9 | 0.535 | Contact Map |
| 3eq2A | 2 | 0.5971 | 99.9 | 0.54 | Contact Map |
| 3breA | 2 | 0.6262 | 99.9 | 0.543 | Contact Map |
| 4l4uA | 1 | 0.6262 | 99.9 | 0.545 | Contact Map |
| 4g97A | 1 | 0.8398 | 99.9 | 0.547 | Contact Map |
| 2ayxA | 1 | 0.9806 | 99.9 | 0.553 | Contact Map |
| 1jbeA | 1 | 0.6068 | 99.9 | 0.554 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)