| cI_L |
| ID: | 1436819385 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 613 (601) | ||
| Sequences: | 3771 (1947.2) | ||
| Seq/Len: | 6.275 | ||
| Nf(neff/√len): | 79.4 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.275).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 307_V | 362_I | 3.737 | 1.00 |
| 136_L | 269_T | 3.625 | 1.00 |
| 287_G | 324_G | 3.226 | 1.00 |
| 308_L | 349_S | 3.162 | 1.00 |
| 196_N | 199_E | 2.984 | 1.00 |
| 160_D | 244_A | 2.851 | 1.00 |
| 376_Y | 438_H | 2.851 | 1.00 |
| 321_L | 418_G | 2.812 | 1.00 |
| 530_L | 535_W | 2.720 | 1.00 |
| 89_L | 128_V | 2.718 | 1.00 |
| 453_H | 465_T | 2.713 | 1.00 |
| 353_I | 360_Q | 2.661 | 1.00 |
| 39_G | 97_F | 2.622 | 1.00 |
| 194_T | 199_E | 2.580 | 1.00 |
| 136_L | 200_M | 2.564 | 1.00 |
| 453_H | 456_P | 2.548 | 1.00 |
| 383_G | 429_T | 2.541 | 1.00 |
| 173_V | 237_T | 2.525 | 1.00 |
| 169_K | 241_D | 2.448 | 1.00 |
| 129_V | 142_G | 2.399 | 1.00 |
| 163_N | 244_A | 2.354 | 1.00 |
| 359_E | 364_K | 2.348 | 1.00 |
| 82_D | 268_R | 2.327 | 1.00 |
| 133_A | 138_L | 2.326 | 1.00 |
| 233_L | 289_V | 2.311 | 1.00 |
| 89_L | 132_L | 2.299 | 1.00 |
| 362_I | 436_V | 2.277 | 1.00 |
| 384_A | 389_L | 2.238 | 1.00 |
| 160_D | 163_N | 2.169 | 1.00 |
| 456_P | 460_L | 2.169 | 1.00 |
| 351_S | 448_V | 2.153 | 1.00 |
| 389_L | 392_V | 2.136 | 1.00 |
| 155_G | 167_A | 2.131 | 1.00 |
| 194_T | 200_M | 2.052 | 1.00 |
| 184_A | 225_G | 2.021 | 1.00 |
| 42_A | 94_G | 2.004 | 1.00 |
| 504_G | 508_A | 1.997 | 1.00 |
| 380_L | 433_I | 1.994 | 1.00 |
| 146_V | 258_M | 1.977 | 1.00 |
| 337_T | 394_A | 1.971 | 1.00 |
| 302_T | 363_F | 1.969 | 1.00 |
| 420_V | 424_M | 1.925 | 1.00 |
| 403_L | 422_A | 1.903 | 1.00 |
| 18_L | 34_G | 1.894 | 1.00 |
| 300_V | 531_L | 1.887 | 1.00 |
| 49_G | 90_S | 1.864 | 1.00 |
| 91_V | 336_M | 1.846 | 1.00 |
| 91_V | 339_A | 1.839 | 1.00 |
| 403_L | 418_G | 1.834 | 1.00 |
| 223_L | 279_V | 1.825 | 1.00 |
| 110_E | 157_Y | 1.814 | 1.00 |
| 296_F | 535_W | 1.812 | 1.00 |
| 362_I | 435_I | 1.812 | 1.00 |
| 14_G | 37_S | 1.789 | 1.00 |
| 267_A | 332_I | 1.789 | 1.00 |
| 372_I | 375_V | 1.783 | 1.00 |
| 323_L | 331_A | 1.776 | 1.00 |
| 589_G | 594_Y | 1.772 | 1.00 |
| 283_V | 324_G | 1.766 | 1.00 |
| 333_F | 398_S | 1.750 | 1.00 |
| 344_L | 382_G | 1.749 | 1.00 |
| 375_V | 447_A | 1.747 | 1.00 |
| 228_G | 235_L | 1.739 | 1.00 |
| 85_S | 264_Y | 1.731 | 1.00 |
| 430_F | 434_F | 1.726 | 1.00 |
| 369_R | 376_Y | 1.722 | 1.00 |
| 42_A | 46_A | 1.719 | 1.00 |
| 168_M | 172_V | 1.717 | 1.00 |
| 112_G | 115_R | 1.700 | 1.00 |
| 142_G | 145_G | 1.686 | 1.00 |
| 266_I | 273_F | 1.671 | 1.00 |
| 96_G | 124_I | 1.671 | 1.00 |
| 220_L | 282_L | 1.653 | 1.00 |
| 416_V | 420_V | 1.647 | 1.00 |
| 292_L | 421_G | 1.633 | 1.00 |
| 219_T | 279_V | 1.632 | 1.00 |
| 162_K | 244_A | 1.629 | 1.00 |
| 465_T | 468_G | 1.615 | 1.00 |
| 299_L | 427_L | 1.604 | 1.00 |
| 456_P | 465_T | 1.595 | 1.00 |
| 92_V | 131_V | 1.581 | 0.99 |
| 267_A | 323_L | 1.579 | 0.99 |
| 266_I | 323_L | 1.525 | 0.99 |
| 453_H | 460_L | 1.519 | 0.99 |
| 453_H | 458_I | 1.518 | 0.99 |
| 288_A | 414_L | 1.512 | 0.99 |
| 35_V | 101_M | 1.505 | 0.99 |
| 126_S | 145_G | 1.505 | 0.99 |
| 573_A | 577_R | 1.497 | 0.99 |
| 12_L | 125_A | 1.493 | 0.99 |
| 87_T | 336_M | 1.491 | 0.99 |
| 181_L | 265_L | 1.489 | 0.99 |
| 497_S | 501_A | 1.488 | 0.99 |
| 68_M | 75_I | 1.476 | 0.99 |
| 191_E | 218_A | 1.467 | 0.99 |
| 354_L | 358_H | 1.465 | 0.99 |
| 46_A | 94_G | 1.463 | 0.99 |
| 219_T | 273_F | 1.462 | 0.99 |
| 289_V | 413_N | 1.461 | 0.99 |
| 334_H | 398_S | 1.445 | 0.99 |
| 287_G | 317_G | 1.445 | 0.99 |
| 183_F | 221_M | 1.440 | 0.99 |
| 87_T | 332_I | 1.433 | 0.99 |
| 355_A | 372_I | 1.431 | 0.99 |
| 379_F | 432_M | 1.428 | 0.99 |
| 35_V | 97_F | 1.419 | 0.99 |
| 120_T | 249_V | 1.416 | 0.99 |
| 315_Q | 425_T | 1.388 | 0.98 |
| 429_T | 432_M | 1.375 | 0.98 |
| 422_A | 500_V | 1.372 | 0.98 |
| 97_F | 101_M | 1.370 | 0.98 |
| 187_I | 218_A | 1.362 | 0.98 |
| 566_N | 570_N | 1.362 | 0.98 |
| 386_L | 429_T | 1.351 | 0.98 |
| 103_A | 116_F | 1.344 | 0.98 |
| 354_L | 446_H | 1.343 | 0.98 |
| 236_Q | 541_F | 1.341 | 0.98 |
| 356_C | 365_M | 1.340 | 0.98 |
| 227_V | 286_V | 1.334 | 0.98 |
| 371_S | 445_A | 1.328 | 0.98 |
| 180_F | 224_G | 1.313 | 0.97 |
| 426_S | 501_A | 1.303 | 0.97 |
| 98_L | 343_A | 1.300 | 0.97 |
| 583_L | 587_E | 1.294 | 0.97 |
| 99_I | 347_L | 1.287 | 0.97 |
| 137_L | 195_L | 1.285 | 0.97 |
| 376_Y | 433_I | 1.281 | 0.97 |
| 161_P | 165_A | 1.278 | 0.97 |
| 454_S | 465_T | 1.277 | 0.97 |
| 188_L | 200_M | 1.269 | 0.97 |
| 194_T | 197_F | 1.268 | 0.96 |
| 354_L | 400_D | 1.258 | 0.96 |
| 17_L | 33_V | 1.249 | 0.96 |
| 491_L | 495_I | 1.248 | 0.96 |
| 334_H | 341_F | 1.245 | 0.96 |
| 188_L | 218_A | 1.241 | 0.96 |
| 201_V | 271_G | 1.240 | 0.96 |
| 506_L | 510_W | 1.237 | 0.96 |
| 383_G | 433_I | 1.231 | 0.96 |
| 295_G | 314_S | 1.220 | 0.95 |
| 84_L | 332_I | 1.220 | 0.95 |
| 230_S | 257_T | 1.219 | 0.95 |
| 43_L | 47_F | 1.218 | 0.95 |
| 419_L | 496_T | 1.218 | 0.95 |
| 304_I | 365_M | 1.216 | 0.95 |
| 273_F | 283_V | 1.214 | 0.95 |
| 509_A | 513_L | 1.213 | 0.95 |
| 534_W | 543_W | 1.213 | 0.95 |
| 533_T | 536_Y | 1.209 | 0.95 |
| 136_L | 188_L | 1.208 | 0.95 |
| 216_M | 276_T | 1.206 | 0.95 |
| 315_Q | 334_H | 1.205 | 0.95 |
| 378_C | 458_I | 1.205 | 0.95 |
| 220_L | 224_G | 1.200 | 0.95 |
| 570_N | 573_A | 1.195 | 0.95 |
| 368_L | 372_I | 1.190 | 0.94 |
| 502_V | 506_L | 1.189 | 0.94 |
| 291_L | 421_G | 1.187 | 0.94 |
| 99_I | 121_N | 1.176 | 0.94 |
| 295_G | 421_G | 1.176 | 0.94 |
| 223_L | 283_V | 1.175 | 0.94 |
| 503_V | 507_L | 1.171 | 0.94 |
| 458_I | 465_T | 1.169 | 0.94 |
| 94_G | 98_L | 1.162 | 0.94 |
| 546_D | 550_V | 1.161 | 0.93 |
| 296_F | 424_M | 1.159 | 0.93 |
| 105_W | 452_T | 1.158 | 0.93 |
| 352_V | 368_L | 1.156 | 0.93 |
| 200_M | 269_T | 1.156 | 0.93 |
| 460_L | 465_T | 1.156 | 0.93 |
| 348_A | 378_C | 1.155 | 0.93 |
| 499_V | 503_V | 1.154 | 0.93 |
| 174_T | 360_Q | 1.150 | 0.93 |
| 415_M | 493_L | 1.149 | 0.93 |
| 217_W | 221_M | 1.149 | 0.93 |
| 284_G | 321_L | 1.148 | 0.93 |
| 5_A | 8_I | 1.147 | 0.93 |
| 337_T | 398_S | 1.146 | 0.93 |
| 362_I | 365_M | 1.145 | 0.93 |
| 453_H | 468_G | 1.144 | 0.93 |
| 427_L | 507_L | 1.139 | 0.93 |
| 403_L | 419_L | 1.137 | 0.93 |
| 219_T | 272_L | 1.136 | 0.93 |
| 103_A | 120_T | 1.133 | 0.92 |
| 311_S | 428_Y | 1.132 | 0.92 |
| 392_V | 397_F | 1.130 | 0.92 |
| 303_D | 360_Q | 1.128 | 0.92 |
| 36_G | 40_L | 1.128 | 0.92 |
| 352_V | 379_F | 1.127 | 0.92 |
| 344_L | 457_L | 1.125 | 0.92 |
| 263_V | 320_F | 1.124 | 0.92 |
| 430_F | 505_I | 1.119 | 0.92 |
| 216_M | 278_E | 1.115 | 0.92 |
| 458_I | 461_L | 1.112 | 0.91 |
| 123_F | 127_M | 1.110 | 0.91 |
| 308_L | 429_T | 1.110 | 0.91 |
| 429_T | 433_I | 1.109 | 0.91 |
| 555_G | 559_L | 1.107 | 0.91 |
| 400_D | 404_A | 1.105 | 0.91 |
| 459_V | 463_L | 1.105 | 0.91 |
| 66_T | 74_N | 1.101 | 0.91 |
| 81_L | 86_L | 1.099 | 0.91 |
| 237_T | 545_Y | 1.095 | 0.91 |
| 276_T | 279_V | 1.095 | 0.91 |
| 284_G | 414_L | 1.093 | 0.90 |
| 383_G | 387_S | 1.093 | 0.90 |
| 531_L | 535_W | 1.093 | 0.90 |
| 81_L | 85_S | 1.092 | 0.90 |
| 75_I | 137_L | 1.088 | 0.90 |
| 7_T | 44_V | 1.084 | 0.90 |
| 93_T | 128_V | 1.084 | 0.90 |
| 528_G | 534_W | 1.079 | 0.90 |
| 129_V | 145_G | 1.079 | 0.90 |
| 450_G | 453_H | 1.077 | 0.90 |
| 389_L | 501_A | 1.076 | 0.90 |
| 181_L | 222_L | 1.074 | 0.89 |
| 187_I | 191_E | 1.074 | 0.89 |
| 426_S | 505_I | 1.074 | 0.89 |
| 27_E | 31_A | 1.073 | 0.89 |
| 31_A | 35_V | 1.072 | 0.89 |
| 127_M | 257_T | 1.071 | 0.89 |
| 215_L | 276_T | 1.070 | 0.89 |
| 454_S | 458_I | 1.068 | 0.89 |
| 21_S | 26_S | 1.066 | 0.89 |
| 401_E | 404_A | 1.066 | 0.89 |
| 257_T | 261_A | 1.065 | 0.89 |
| 292_L | 296_F | 1.062 | 0.89 |
| 394_A | 464_S | 1.060 | 0.89 |
| 220_L | 279_V | 1.060 | 0.89 |
| 579_A | 583_L | 1.059 | 0.89 |
| 564_P | 568_M | 1.059 | 0.89 |
| 216_M | 279_V | 1.058 | 0.88 |
| 7_T | 48_I | 1.058 | 0.88 |
| 251_A | 347_L | 1.057 | 0.88 |
| 423_F | 504_G | 1.057 | 0.88 |
| 26_S | 29_V | 1.055 | 0.88 |
| 302_T | 361_N | 1.055 | 0.88 |
| 457_L | 465_T | 1.055 | 0.88 |
| 109_G | 358_H | 1.054 | 0.88 |
| 318_Y | 422_A | 1.053 | 0.88 |
| 266_I | 320_F | 1.052 | 0.88 |
| 107_M | 110_E | 1.050 | 0.88 |
| 38_V | 41_A | 1.050 | 0.88 |
| 12_L | 16_V | 1.049 | 0.88 |
| 227_V | 324_G | 1.047 | 0.88 |
| 549_F | 553_F | 1.045 | 0.88 |
| 544_L | 548_V | 1.044 | 0.88 |
| 5_A | 9_I | 1.042 | 0.87 |
| 232_Q | 243_M | 1.040 | 0.87 |
| 421_G | 424_M | 1.037 | 0.87 |
| 406_A | 495_I | 1.037 | 0.87 |
| 588_N | 593_W | 1.037 | 0.87 |
| 61_S | 134_D | 1.036 | 0.87 |
| 333_F | 337_T | 1.036 | 0.87 |
| 263_V | 323_L | 1.035 | 0.87 |
| 184_A | 188_L | 1.035 | 0.87 |
| 574_V | 578_F | 1.034 | 0.87 |
| 450_G | 468_G | 1.033 | 0.87 |
| 233_L | 541_F | 1.031 | 0.87 |
| 554_L | 557_A | 1.031 | 0.87 |
| 6_L | 10_L | 1.031 | 0.87 |
| 341_F | 395_G | 1.030 | 0.87 |
| 65_W | 568_M | 1.028 | 0.87 |
| 100_H | 120_T | 1.027 | 0.86 |
| 240_A | 310_Y | 1.026 | 0.86 |
| 284_G | 324_G | 1.025 | 0.86 |
| 383_G | 386_L | 1.024 | 0.86 |
| 325_V | 406_A | 1.015 | 0.86 |
| 362_I | 432_M | 1.012 | 0.85 |
| 40_L | 43_L | 1.012 | 0.85 |
| 433_I | 438_H | 1.012 | 0.85 |
| 99_I | 142_G | 1.010 | 0.85 |
| 29_V | 32_I | 1.008 | 0.85 |
| 187_I | 217_W | 1.006 | 0.85 |
| 574_V | 582_G | 1.002 | 0.85 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3rkoL | 2 | 0.9902 | 100 | 0.252 | Contact Map |
| 4he8L | 2 | 0.956 | 100 | 0.291 | Contact Map |
| 3rkoM | 2 | 0.7781 | 100 | 0.485 | Contact Map |
| 4he8M | 2 | 0.7488 | 100 | 0.486 | Contact Map |
| 3rkoN | 2 | 0.7341 | 100 | 0.53 | Contact Map |
| 4he8N | 2 | 0.6933 | 100 | 0.578 | Contact Map |
| 4he8K | 2 | 0.1517 | 42.2 | 0.979 | Contact Map |
| 3rkoK | 2 | 0.1582 | 23.2 | 0.982 | Contact Map |
| 1s5lX | 2 | 0.0636 | 3.8 | 0.987 | Contact Map |
| 2kv5A | 1 | 0.0538 | 3.5 | 0.988 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)