| 1aon1xck |
| ID: | 1433330176 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 524 (524) | ||
| Sequences: | 3402 (1549.7) | ||
| Seq/Len: | 6.492 | ||
| Nf(neff/√len): | 67.7 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.492).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 130_L | 496_T | 4.685 | 1.00 |
| 125_A | 128_E | 4.246 | 1.00 |
| 486_N | 489_D | 4.190 | 1.00 |
| 134_S | 496_T | 4.149 | 1.00 |
| 273_A | 324_I | 3.820 | 1.00 |
| 478_N | 485_G | 3.535 | 1.00 |
| 140_S | 163_E | 3.462 | 1.00 |
| 129_E | 132_A | 3.423 | 1.00 |
| 478_N | 481_T | 3.395 | 1.00 |
| 464_V | 484_Y | 3.366 | 1.00 |
| 144_A | 159_K | 3.114 | 1.00 |
| 159_K | 163_E | 3.098 | 1.00 |
| 485_G | 490_M | 2.994 | 1.00 |
| 221_L | 297_G | 2.973 | 1.00 |
| 431_Q | 439_I | 2.841 | 1.00 |
| 123_V | 503_L | 2.839 | 1.00 |
| 477_Y | 482_E | 2.716 | 1.00 |
| 50_K | 55_V | 2.715 | 1.00 |
| 121_K | 435_Q | 2.683 | 1.00 |
| 140_S | 166_D | 2.569 | 1.00 |
| 101_E | 444_R | 2.552 | 1.00 |
| 459_E | 477_Y | 2.537 | 1.00 |
| 418_L | 499_T | 2.532 | 1.00 |
| 320_K | 334_G | 2.526 | 1.00 |
| 98_I | 119_I | 2.523 | 1.00 |
| 450_L | 468_V | 2.446 | 1.00 |
| 425_L | 439_I | 2.419 | 1.00 |
| 119_I | 507_A | 2.339 | 1.00 |
| 123_V | 507_A | 2.323 | 1.00 |
| 130_L | 417_A | 2.234 | 1.00 |
| 414_G | 487_M | 2.182 | 1.00 |
| 128_E | 132_A | 2.122 | 1.00 |
| 20_N | 24_D | 2.120 | 1.00 |
| 213_E | 321_R | 2.096 | 1.00 |
| 338_E | 342_Q | 2.054 | 1.00 |
| 90_T | 502_A | 2.033 | 1.00 |
| 221_L | 291_I | 2.024 | 1.00 |
| 447_E | 469_K | 2.015 | 1.00 |
| 120_D | 511_G | 2.015 | 1.00 |
| 71_Q | 74_K | 2.005 | 1.00 |
| 467_T | 470_G | 2.001 | 1.00 |
| 337_E | 340_A | 1.998 | 1.00 |
| 186_L | 380_V | 1.968 | 1.00 |
| 410_V | 493_L | 1.965 | 1.00 |
| 125_A | 428_L | 1.961 | 1.00 |
| 161_I | 395_V | 1.960 | 1.00 |
| 474_N | 486_N | 1.940 | 1.00 |
| 478_N | 490_M | 1.934 | 1.00 |
| 358_D | 361_R | 1.926 | 1.00 |
| 454_V | 459_E | 1.922 | 1.00 |
| 426_A | 443_L | 1.914 | 1.00 |
| 393_A | 396_E | 1.883 | 1.00 |
| 98_I | 442_A | 1.879 | 1.00 |
| 167_K | 190_E | 1.871 | 1.00 |
| 219_I | 319_A | 1.854 | 1.00 |
| 355_A | 361_R | 1.835 | 1.00 |
| 5_V | 518_C | 1.829 | 1.00 |
| 128_E | 131_K | 1.822 | 1.00 |
| 142_A | 145_Q | 1.820 | 1.00 |
| 131_K | 500_R | 1.793 | 1.00 |
| 459_E | 482_E | 1.781 | 1.00 |
| 126_A | 421_V | 1.777 | 1.00 |
| 129_E | 424_K | 1.766 | 1.00 |
| 471_G | 475_Y | 1.746 | 1.00 |
| 17_R | 66_E | 1.745 | 1.00 |
| 127_V | 131_K | 1.744 | 1.00 |
| 99_I | 513_M | 1.740 | 1.00 |
| 126_A | 446_M | 1.708 | 1.00 |
| 216_S | 244_K | 1.693 | 1.00 |
| 27_K | 96_Q | 1.690 | 1.00 |
| 325_N | 328_T | 1.680 | 1.00 |
| 124_T | 128_E | 1.676 | 1.00 |
| 442_A | 507_A | 1.673 | 1.00 |
| 148_T | 155_E | 1.668 | 1.00 |
| 446_M | 503_L | 1.661 | 1.00 |
| 286_A | 289_Q | 1.659 | 1.00 |
| 16_L | 20_N | 1.648 | 1.00 |
| 298_T | 315_D | 1.644 | 1.00 |
| 97_A | 100_T | 1.642 | 1.00 |
| 165_M | 406_V | 1.633 | 1.00 |
| 342_Q | 346_A | 1.632 | 1.00 |
| 23_A | 96_Q | 1.627 | 1.00 |
| 14_K | 17_R | 1.620 | 1.00 |
| 228_N | 231_E | 1.616 | 1.00 |
| 125_A | 424_K | 1.613 | 1.00 |
| 425_L | 443_L | 1.611 | 1.00 |
| 81_N | 393_A | 1.610 | 1.00 |
| 216_S | 320_K | 1.609 | 1.00 |
| 485_G | 492_I | 1.609 | 1.00 |
| 20_N | 96_Q | 1.599 | 1.00 |
| 102_G | 119_I | 1.590 | 1.00 |
| 215_E | 321_R | 1.576 | 0.99 |
| 419_I | 469_K | 1.574 | 0.99 |
| 116_K | 120_D | 1.571 | 0.99 |
| 378_I | 395_V | 1.556 | 0.99 |
| 319_A | 332_I | 1.556 | 0.99 |
| 17_R | 21_V | 1.551 | 0.99 |
| 237_E | 241_K | 1.542 | 0.99 |
| 218_F | 318_Q | 1.539 | 0.99 |
| 214_L | 247_L | 1.537 | 0.99 |
| 249_I | 291_I | 1.537 | 0.99 |
| 155_E | 159_K | 1.536 | 0.99 |
| 348_I | 368_V | 1.535 | 0.99 |
| 105_A | 110_M | 1.532 | 0.99 |
| 216_S | 318_Q | 1.529 | 0.99 |
| 47_T | 393_A | 1.525 | 0.99 |
| 139_D | 142_A | 1.520 | 0.99 |
| 129_E | 133_L | 1.510 | 0.99 |
| 300_I | 311_A | 1.504 | 0.99 |
| 81_N | 396_E | 1.494 | 0.99 |
| 447_E | 451_R | 1.493 | 0.99 |
| 4_D | 523_L | 1.483 | 0.99 |
| 467_T | 483_E | 1.482 | 0.99 |
| 81_N | 400_H | 1.473 | 0.99 |
| 140_S | 159_K | 1.473 | 0.99 |
| 428_L | 439_I | 1.465 | 0.99 |
| 448_A | 452_Q | 1.464 | 0.99 |
| 312_T | 315_D | 1.453 | 0.99 |
| 50_K | 393_A | 1.424 | 0.99 |
| 114_D | 435_Q | 1.422 | 0.99 |
| 238_A | 241_K | 1.411 | 0.99 |
| 382_A | 388_M | 1.399 | 0.99 |
| 221_L | 299_V | 1.388 | 0.98 |
| 214_L | 217_P | 1.382 | 0.98 |
| 431_Q | 435_Q | 1.374 | 0.98 |
| 105_A | 437_V | 1.370 | 0.98 |
| 160_L | 186_L | 1.366 | 0.98 |
| 417_A | 421_V | 1.364 | 0.98 |
| 453_I | 479_A | 1.361 | 0.98 |
| 416_V | 420_R | 1.354 | 0.98 |
| 9_N | 13_V | 1.350 | 0.98 |
| 15_M | 18_G | 1.348 | 0.98 |
| 292_A | 299_V | 1.335 | 0.98 |
| 220_L | 311_A | 1.335 | 0.98 |
| 160_L | 378_I | 1.335 | 0.98 |
| 454_V | 461_P | 1.324 | 0.98 |
| 424_K | 427_D | 1.317 | 0.98 |
| 187_D | 379_K | 1.313 | 0.97 |
| 259_A | 263_V | 1.303 | 0.97 |
| 423_S | 427_D | 1.297 | 0.97 |
| 212_V | 273_A | 1.292 | 0.97 |
| 352_I | 368_V | 1.287 | 0.97 |
| 100_T | 448_A | 1.284 | 0.97 |
| 119_I | 442_A | 1.282 | 0.97 |
| 339_A | 342_Q | 1.280 | 0.97 |
| 362_E | 365_Q | 1.268 | 0.97 |
| 135_V | 488_I | 1.253 | 0.96 |
| 76_V | 91_A | 1.252 | 0.96 |
| 217_P | 319_A | 1.250 | 0.96 |
| 487_M | 492_I | 1.246 | 0.96 |
| 45_A | 386_V | 1.245 | 0.96 |
| 408_E | 497_K | 1.242 | 0.96 |
| 126_A | 425_L | 1.241 | 0.96 |
| 340_A | 343_G | 1.239 | 0.96 |
| 178_D | 389_K | 1.232 | 0.96 |
| 233_L | 237_E | 1.232 | 0.96 |
| 39_L | 520_V | 1.230 | 0.96 |
| 475_Y | 484_Y | 1.229 | 0.96 |
| 436_N | 440_K | 1.229 | 0.96 |
| 130_L | 421_V | 1.227 | 0.96 |
| 130_L | 500_R | 1.226 | 0.96 |
| 49_T | 390_E | 1.226 | 0.96 |
| 350_Q | 354_E | 1.223 | 0.96 |
| 125_A | 439_I | 1.218 | 0.96 |
| 90_T | 449_P | 1.205 | 0.95 |
| 238_A | 312_T | 1.202 | 0.95 |
| 51_D | 54_S | 1.199 | 0.95 |
| 265_T | 271_K | 1.199 | 0.95 |
| 117_R | 435_Q | 1.196 | 0.95 |
| 500_R | 504_Q | 1.192 | 0.95 |
| 68_M | 519_M | 1.189 | 0.95 |
| 420_R | 473_G | 1.188 | 0.95 |
| 163_E | 190_E | 1.187 | 0.95 |
| 125_A | 129_E | 1.183 | 0.95 |
| 52_G | 89_T | 1.178 | 0.94 |
| 363_K | 366_E | 1.175 | 0.94 |
| 352_I | 358_D | 1.174 | 0.94 |
| 256_E | 260_T | 1.170 | 0.94 |
| 322_V | 331_I | 1.159 | 0.94 |
| 146_V | 410_V | 1.157 | 0.94 |
| 20_N | 100_T | 1.156 | 0.94 |
| 265_T | 268_G | 1.156 | 0.94 |
| 441_V | 444_R | 1.155 | 0.94 |
| 68_M | 521_T | 1.154 | 0.94 |
| 319_A | 331_I | 1.145 | 0.93 |
| 122_A | 442_A | 1.145 | 0.93 |
| 346_A | 350_Q | 1.137 | 0.93 |
| 412_A | 416_V | 1.136 | 0.93 |
| 422_A | 469_K | 1.132 | 0.93 |
| 4_D | 522_D | 1.128 | 0.93 |
| 97_A | 448_A | 1.124 | 0.92 |
| 127_V | 504_Q | 1.122 | 0.92 |
| 143_I | 147_G | 1.118 | 0.92 |
| 138_S | 142_A | 1.118 | 0.92 |
| 118_G | 122_A | 1.116 | 0.92 |
| 121_K | 125_A | 1.113 | 0.92 |
| 132_A | 424_K | 1.107 | 0.92 |
| 180_T | 183_Q | 1.105 | 0.92 |
| 4_D | 521_T | 1.104 | 0.92 |
| 239_V | 242_A | 1.097 | 0.91 |
| 145_Q | 491_G | 1.088 | 0.91 |
| 380_V | 388_M | 1.087 | 0.91 |
| 345_V | 349_R | 1.085 | 0.91 |
| 127_V | 500_R | 1.085 | 0.91 |
| 101_E | 104_K | 1.079 | 0.90 |
| 42_S | 64_K | 1.078 | 0.90 |
| 412_A | 488_I | 1.077 | 0.90 |
| 214_L | 246_L | 1.072 | 0.90 |
| 101_E | 445_A | 1.072 | 0.90 |
| 320_K | 333_D | 1.065 | 0.89 |
| 419_I | 450_L | 1.055 | 0.89 |
| 440_K | 444_R | 1.054 | 0.89 |
| 55_V | 393_A | 1.054 | 0.89 |
| 135_V | 142_A | 1.053 | 0.89 |
| 206_K | 209_T | 1.051 | 0.89 |
| 307_E | 310_K | 1.051 | 0.89 |
| 179_G | 182_L | 1.042 | 0.88 |
| 12_R | 18_G | 1.038 | 0.88 |
| 459_E | 479_A | 1.038 | 0.88 |
| 407_E | 497_K | 1.033 | 0.87 |
| 417_A | 496_T | 1.033 | 0.87 |
| 19_V | 513_M | 1.033 | 0.87 |
| 432_N | 439_I | 1.032 | 0.87 |
| 16_L | 99_I | 1.031 | 0.87 |
| 248_I | 272_V | 1.028 | 0.87 |
| 49_T | 55_V | 1.026 | 0.87 |
| 19_V | 73_V | 1.025 | 0.87 |
| 253_V | 274_A | 1.024 | 0.87 |
| 316_L | 398_A | 1.022 | 0.87 |
| 320_K | 335_V | 1.019 | 0.87 |
| 349_R | 372_A | 1.016 | 0.86 |
| 27_K | 452_Q | 1.011 | 0.86 |
| 122_A | 439_I | 1.008 | 0.86 |
| 346_A | 349_R | 1.005 | 0.85 |
| 224_K | 302_E | 1.000 | 0.85 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1kp8A | 14 | 1 | 100 | 0.021 | Contact Map |
| 1iokA | 7 | 0.9179 | 100 | 0.022 | Contact Map |
| 3rtkA | 2 | 0.8569 | 100 | 0.025 | Contact Map |
| 1a6dB | 1 | 0.8588 | 100 | 0.177 | Contact Map |
| 1q3qA | 4 | 0.8798 | 100 | 0.178 | Contact Map |
| 3ko1A | 9 | 0.8626 | 100 | 0.178 | Contact Map |
| 1a6dA | 1 | 0.8645 | 100 | 0.179 | Contact Map |
| 3ruvA | 4 | 0.8779 | 100 | 0.181 | Contact Map |
| 3j1bA | 16 | 0.8721 | 100 | 0.181 | Contact Map |
| 3iygA | 1 | 0.8893 | 100 | 0.183 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)