| ELAV4_HUMAN |
| ID: | 1430407653 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 71 (71) | ||
| Sequences: | 12912 (8076) | ||
| Seq/Len: | 181.859 | ||
| Nf(neff/√len): | 958.4 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 181.859).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 49_D | 52_D | 2.515 | 1.00 |
| 24_E | 48_I | 2.396 | 1.00 |
| 11_T | 14_E | 2.369 | 1.00 |
| 22_I | 59_T | 2.266 | 1.00 |
| 26_E | 48_I | 2.125 | 1.00 |
| 14_E | 66_Q | 2.106 | 1.00 |
| 55_K | 59_T | 1.997 | 1.00 |
| 8_Q | 68_K | 1.818 | 1.00 |
| 22_I | 60_L | 1.733 | 1.00 |
| 50_P | 54_E | 1.614 | 1.00 |
| 1_L | 57_I | 1.602 | 1.00 |
| 33_D | 36_T | 1.585 | 1.00 |
| 27_S | 46_N | 1.580 | 1.00 |
| 16_R | 20_G | 1.559 | 1.00 |
| 64_R | 69_T | 1.533 | 1.00 |
| 24_E | 52_D | 1.489 | 1.00 |
| 4_N | 71_K | 1.429 | 1.00 |
| 26_E | 46_N | 1.428 | 1.00 |
| 65_L | 70_I | 1.425 | 1.00 |
| 31_V | 42_Y | 1.423 | 1.00 |
| 7_P | 10_M | 1.417 | 1.00 |
| 19_F | 45_V | 1.413 | 1.00 |
| 12_Q | 16_R | 1.410 | 1.00 |
| 9_N | 66_Q | 1.341 | 0.99 |
| 18_L | 60_L | 1.326 | 0.99 |
| 18_L | 63_L | 1.302 | 0.99 |
| 48_I | 52_D | 1.298 | 0.99 |
| 62_G | 69_T | 1.298 | 0.99 |
| 15_F | 43_G | 1.288 | 0.99 |
| 30_L | 39_S | 1.287 | 0.99 |
| 7_P | 66_Q | 1.227 | 0.98 |
| 8_Q | 38_Q | 1.214 | 0.98 |
| 10_M | 65_L | 1.168 | 0.97 |
| 64_R | 67_T | 1.152 | 0.97 |
| 54_E | 58_N | 1.135 | 0.97 |
| 31_V | 44_F | 1.135 | 0.97 |
| 3_V | 19_F | 1.102 | 0.96 |
| 60_L | 63_L | 1.096 | 0.96 |
| 51_K | 55_K | 1.095 | 0.96 |
| 17_S | 20_G | 1.060 | 0.95 |
| 18_L | 65_L | 1.057 | 0.95 |
| 47_Y | 56_A | 1.022 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3pgwS | 2 | 1 | 99.5 | -0.088 | Contact Map |
| 2x1fA | 1 | 1 | 99.5 | -0.079 | Contact Map |
| 3bs9A | 2 | 1 | 99.5 | -0.078 | Contact Map |
| 3q2sC | 2 | 1 | 99.5 | -0.06 | Contact Map |
| 2rs2A | 1 | 0.9859 | 99.5 | -0.052 | Contact Map |
| 2yh0A | 1 | 1 | 99.5 | -0.05 | Contact Map |
| 3n9uC | 2 | 1 | 99.5 | -0.045 | Contact Map |
| 2ywkA | 1 | 0.9859 | 99.5 | -0.041 | Contact Map |
| 1rk8A | 1 | 1 | 99.4 | -0.033 | Contact Map |
| 2mjnA | 1 | 1 | 99.4 | -0.029 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)