| ank3 |
| ID: | 1430320011 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 99 (98) | ||
| Sequences: | 34310 (27611.1) | ||
| Seq/Len: | 350.102 | ||
| Nf(neff/√len): | 2789.1 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 350.102).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 51_A | 83_I | 2.350 | 1.00 |
| 19_K | 53_L | 2.332 | 1.00 |
| 18_A | 50_T | 2.279 | 1.00 |
| 52_K | 86_L | 2.195 | 1.00 |
| 53_L | 57_H | 2.184 | 1.00 |
| 20_L | 24_H | 2.168 | 1.00 |
| 31_K | 63_E | 2.041 | 1.00 |
| 64_K | 96_E | 1.961 | 1.00 |
| 86_L | 90_H | 1.858 | 1.00 |
| 36_K | 65_D | 1.763 | 1.00 |
| 69_Q | 98_D | 1.674 | 1.00 |
| 15_K | 53_L | 1.671 | 1.00 |
| 13_N | 47_S | 1.661 | 1.00 |
| 46_N | 80_S | 1.599 | 1.00 |
| 3_R | 32_D | 1.598 | 1.00 |
| 48_K | 86_L | 1.566 | 1.00 |
| 14_S | 17_T | 1.447 | 1.00 |
| 15_K | 49_E | 1.412 | 1.00 |
| 48_K | 82_E | 1.366 | 0.99 |
| 39_L | 51_A | 1.362 | 0.99 |
| 69_Q | 74_E | 1.334 | 0.99 |
| 7_H | 41_I | 1.310 | 0.99 |
| 40_H | 74_E | 1.300 | 0.99 |
| 73_F | 76_A | 1.292 | 0.99 |
| 72_L | 84_A | 1.268 | 0.99 |
| 3_R | 11_S | 1.259 | 0.99 |
| 81_R | 85_E | 1.227 | 0.98 |
| 69_Q | 77_F | 1.199 | 0.98 |
| 36_K | 41_I | 1.199 | 0.98 |
| 15_K | 50_T | 1.189 | 0.98 |
| 22_I | 53_L | 1.163 | 0.97 |
| 17_T | 20_L | 1.159 | 0.97 |
| 36_K | 44_S | 1.155 | 0.97 |
| 47_S | 50_T | 1.149 | 0.97 |
| 6_L | 18_A | 1.143 | 0.97 |
| 80_S | 83_I | 1.121 | 0.97 |
| 48_K | 52_K | 1.109 | 0.96 |
| 40_H | 73_F | 1.107 | 0.96 |
| 55_I | 86_L | 1.097 | 0.96 |
| 42_A | 51_A | 1.089 | 0.96 |
| 9_A | 18_A | 1.087 | 0.96 |
| 69_Q | 73_F | 1.086 | 0.96 |
| 15_K | 19_K | 1.080 | 0.95 |
| 48_K | 83_I | 1.072 | 0.95 |
| 60_N | 63_E | 1.058 | 0.95 |
| 72_L | 88_I | 1.053 | 0.95 |
| 3_R | 8_F | 1.053 | 0.95 |
| 55_I | 61_I | 1.049 | 0.94 |
| 76_A | 88_I | 1.037 | 0.94 |
| 22_I | 57_H | 1.037 | 0.94 |
| 55_I | 90_H | 1.037 | 0.94 |
| 8_F | 12_H | 1.036 | 0.94 |
| 93_N | 96_E | 1.021 | 0.93 |
| 75_A | 84_A | 1.017 | 0.93 |
| 65_D | 69_Q | 1.012 | 0.93 |
| 22_I | 28_I | 1.009 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4rlvA | 1 | 1 | 99.7 | -0.132 | Contact Map |
| 4cj9A | 2 | 1 | 99.6 | -0.127 | Contact Map |
| 4oavB | 2 | 1 | 99.6 | -0.125 | Contact Map |
| 3lvqE | 1 | 0.9293 | 99.6 | -0.121 | Contact Map |
| 3aaaC | 1 | 0.9899 | 99.6 | -0.11 | Contact Map |
| 4xczA | 1 | 1 | 99.6 | -0.103 | Contact Map |
| 1oy3D | 1 | 0.9899 | 99.6 | -0.094 | Contact Map |
| 2vgeA | 1 | 1 | 99.6 | -0.087 | Contact Map |
| 3ehrA | 2 | 1 | 99.6 | -0.083 | Contact Map |
| 1n11A | 1 | 0.9798 | 99.6 | -0.081 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)