| ftsZ |
| ID: | 1427887212 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 383 (319) | ||
| Sequences: | 1280 (711.4) | ||
| Seq/Len: | 4.013 | ||
| Nf(neff/√len): | 39.8 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 4.013).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 42_V | 60_I | 4.241 | 1.00 |
| 78_R | 152_E | 3.666 | 1.00 |
| 110_T | 149_G | 3.614 | 1.00 |
| 18_V | 112_A | 3.287 | 1.00 |
| 85_R | 118_E | 3.267 | 1.00 |
| 229_V | 305_E | 3.223 | 1.00 |
| 85_R | 119_V | 3.037 | 1.00 |
| 84_D | 87_A | 2.989 | 1.00 |
| 152_E | 155_K | 2.930 | 1.00 |
| 136_N | 166_D | 2.917 | 1.00 |
| 234_D | 238_E | 2.670 | 1.00 |
| 104_M | 150_I | 2.575 | 1.00 |
| 118_E | 156_H | 2.554 | 1.00 |
| 235_R | 274_E | 2.545 | 1.00 |
| 157_V | 160_L | 2.438 | 1.00 |
| 129_A | 157_V | 2.339 | 1.00 |
| 262_V | 279_G | 2.265 | 1.00 |
| 231_S | 305_E | 2.243 | 1.00 |
| 133_K | 143_M | 2.227 | 1.00 |
| 31_R | 53_A | 2.226 | 1.00 |
| 118_E | 122_D | 2.213 | 1.00 |
| 90_A | 93_E | 2.192 | 1.00 |
| 217_M | 313_T | 2.004 | 1.00 |
| 100_I | 113_A | 1.993 | 1.00 |
| 268_F | 300_P | 1.976 | 1.00 |
| 184_A | 187_D | 1.963 | 1.00 |
| 85_R | 89_R | 1.898 | 1.00 |
| 199_L | 208_V | 1.890 | 1.00 |
| 14_K | 95_A | 1.851 | 1.00 |
| 58_I | 91_A | 1.804 | 1.00 |
| 42_V | 116_V | 1.777 | 0.99 |
| 129_A | 160_L | 1.763 | 0.99 |
| 16_I | 92_L | 1.762 | 0.99 |
| 89_R | 122_D | 1.761 | 0.99 |
| 23_G | 48_A | 1.754 | 0.99 |
| 40_F | 54_V | 1.735 | 0.99 |
| 26_V | 41_A | 1.729 | 0.99 |
| 140_K | 144_A | 1.703 | 0.99 |
| 237_E | 277_T | 1.696 | 0.99 |
| 266_A | 270_L | 1.684 | 0.99 |
| 79_N | 82_D | 1.678 | 0.99 |
| 216_V | 259_G | 1.650 | 0.99 |
| 95_A | 98_V | 1.638 | 0.99 |
| 92_L | 98_V | 1.637 | 0.99 |
| 30_V | 54_V | 1.611 | 0.99 |
| 78_R | 82_D | 1.593 | 0.99 |
| 66_K | 96_D | 1.558 | 0.98 |
| 175_G | 273_D | 1.554 | 0.98 |
| 187_D | 190_K | 1.551 | 0.98 |
| 92_L | 123_L | 1.546 | 0.98 |
| 214_R | 218_S | 1.510 | 0.98 |
| 133_K | 147_E | 1.508 | 0.98 |
| 27_E | 52_T | 1.502 | 0.98 |
| 190_K | 194_Q | 1.501 | 0.98 |
| 258_R | 287_S | 1.496 | 0.98 |
| 238_E | 241_E | 1.486 | 0.97 |
| 244_I | 310_V | 1.471 | 0.97 |
| 176_I | 181_A | 1.461 | 0.97 |
| 151_T | 155_K | 1.457 | 0.97 |
| 269_D | 304_D | 1.447 | 0.97 |
| 159_S | 217_M | 1.445 | 0.97 |
| 81_A | 112_A | 1.431 | 0.97 |
| 100_I | 129_A | 1.418 | 0.96 |
| 229_V | 307_R | 1.412 | 0.96 |
| 28_H | 186_N | 1.395 | 0.96 |
| 270_L | 306_L | 1.380 | 0.95 |
| 275_F | 294_I | 1.369 | 0.95 |
| 87_A | 90_A | 1.368 | 0.95 |
| 237_E | 241_E | 1.353 | 0.95 |
| 27_E | 53_A | 1.347 | 0.95 |
| 240_A | 282_I | 1.343 | 0.95 |
| 72_A | 215_T | 1.331 | 0.94 |
| 270_L | 275_F | 1.329 | 0.94 |
| 133_K | 250_E | 1.313 | 0.94 |
| 259_G | 287_S | 1.308 | 0.93 |
| 25_A | 39_F | 1.307 | 0.93 |
| 177_S | 180_D | 1.307 | 0.93 |
| 75_E | 79_N | 1.298 | 0.93 |
| 119_V | 123_L | 1.297 | 0.93 |
| 89_R | 119_V | 1.288 | 0.93 |
| 205_L | 296_T | 1.280 | 0.92 |
| 41_A | 54_V | 1.271 | 0.92 |
| 95_A | 125_I | 1.259 | 0.92 |
| 25_A | 29_M | 1.255 | 0.92 |
| 172_L | 184_A | 1.253 | 0.91 |
| 40_F | 56_Q | 1.242 | 0.91 |
| 86_D | 90_A | 1.234 | 0.91 |
| 204_G | 297_S | 1.223 | 0.90 |
| 88_L | 119_V | 1.222 | 0.90 |
| 260_V | 286_A | 1.221 | 0.90 |
| 150_I | 162_T | 1.219 | 0.90 |
| 299_D | 307_R | 1.217 | 0.90 |
| 220_M | 227_S | 1.204 | 0.89 |
| 179_L | 298_L | 1.194 | 0.89 |
| 98_V | 125_I | 1.170 | 0.87 |
| 72_A | 293_V | 1.165 | 0.87 |
| 58_I | 88_L | 1.162 | 0.87 |
| 52_T | 57_T | 1.162 | 0.87 |
| 278_V | 308_V | 1.160 | 0.87 |
| 259_G | 290_A | 1.158 | 0.86 |
| 33_R | 308_V | 1.154 | 0.86 |
| 133_K | 311_V | 1.154 | 0.86 |
| 62_S | 87_A | 1.151 | 0.86 |
| 217_M | 259_G | 1.150 | 0.86 |
| 152_E | 156_H | 1.143 | 0.86 |
| 141_K | 145_F | 1.142 | 0.85 |
| 89_R | 123_L | 1.142 | 0.85 |
| 222_Y | 316_G | 1.125 | 0.84 |
| 18_V | 116_V | 1.123 | 0.84 |
| 168_L | 185_A | 1.120 | 0.84 |
| 16_I | 42_V | 1.119 | 0.84 |
| 17_G | 22_G | 1.117 | 0.84 |
| 231_S | 304_D | 1.114 | 0.83 |
| 148_Q | 151_T | 1.112 | 0.83 |
| 14_K | 40_F | 1.111 | 0.83 |
| 98_V | 116_V | 1.108 | 0.83 |
| 148_Q | 152_E | 1.101 | 0.82 |
| 177_S | 271_R | 1.093 | 0.82 |
| 41_A | 52_T | 1.091 | 0.82 |
| 144_A | 148_Q | 1.090 | 0.82 |
| 180_D | 183_G | 1.084 | 0.81 |
| 269_D | 303_N | 1.083 | 0.81 |
| 34_I | 190_K | 1.079 | 0.81 |
| 15_V | 39_F | 1.073 | 0.80 |
| 37_V | 157_V | 1.059 | 0.79 |
| 167_K | 246_S | 1.053 | 0.78 |
| 172_L | 176_I | 1.049 | 0.78 |
| 265_T | 302_M | 1.047 | 0.78 |
| 46_A | 50_R | 1.045 | 0.78 |
| 278_V | 306_L | 1.039 | 0.77 |
| 64_I | 311_V | 1.038 | 0.77 |
| 28_H | 192_A | 1.036 | 0.77 |
| 129_A | 153_L | 1.033 | 0.77 |
| 241_E | 245_S | 1.015 | 0.75 |
| 86_D | 89_R | 1.014 | 0.75 |
| 276_E | 280_N | 1.013 | 0.75 |
| 30_V | 39_F | 1.013 | 0.75 |
| 230_A | 238_E | 1.012 | 0.74 |
| 280_N | 283_R | 1.011 | 0.74 |
| 206_M | 292_V | 1.009 | 0.74 |
| 147_E | 151_T | 1.008 | 0.74 |
| 82_D | 118_E | 1.002 | 0.73 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2vawA | 1 | 0.8198 | 100 | 0.075 | Contact Map |
| 2vxyA | 1 | 0.7963 | 100 | 0.129 | Contact Map |
| 2vapA | 1 | 0.8512 | 100 | 0.21 | Contact Map |
| 2r751 | 1 | 0.8433 | 100 | 0.235 | Contact Map |
| 4m8iA | 1 | 0.812 | 100 | 0.282 | Contact Map |
| 1rq2A | 2 | 0.7859 | 100 | 0.3 | Contact Map |
| 1ofuA | 2 | 0.8016 | 100 | 0.334 | Contact Map |
| 1w5fA | 2 | 0.8146 | 100 | 0.34 | Contact Map |
| 4ei7A | 2 | 0.8747 | 100 | 0.406 | Contact Map |
| 4b45A | 1 | 0.8146 | 100 | 0.431 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)