| A2AR_LABOS_1e_40_iter1 |
| ID: | 1427865511 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 310 (284) | ||
| Sequences: | 1652 (1038.5) | ||
| Seq/Len: | 5.817 | ||
| Nf(neff/√len): | 61.6 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 5.817).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 260_C | 263_Y | 3.984 | 1.00 |
| 46_T | 82_A | 3.466 | 1.00 |
| 57_V | 72_V | 3.176 | 1.00 |
| 98_W | 101_G | 2.208 | 1.00 |
| 166_P | 169_S | 2.036 | 1.00 |
| 47_V | 82_A | 1.861 | 1.00 |
| 215_R | 219_I | 1.849 | 1.00 |
| 103_V | 107_I | 1.812 | 1.00 |
| 73_S | 122_H | 1.788 | 1.00 |
| 125_A | 155_V | 1.751 | 1.00 |
| 66_P | 70_F | 1.706 | 1.00 |
| 65_A | 68_N | 1.686 | 1.00 |
| 126_I | 236_L | 1.686 | 1.00 |
| 82_A | 291_N | 1.681 | 1.00 |
| 150_C | 154_I | 1.672 | 1.00 |
| 77_A | 122_H | 1.665 | 1.00 |
| 242_V | 286_I | 1.645 | 1.00 |
| 46_T | 281_S | 1.616 | 0.99 |
| 69_L | 152_I | 1.600 | 0.99 |
| 56_A | 301_I | 1.597 | 0.99 |
| 54_V | 77_A | 1.595 | 0.99 |
| 81_V | 86_M | 1.576 | 0.99 |
| 38_L | 41_F | 1.558 | 0.99 |
| 61_R | 144_T | 1.551 | 0.99 |
| 201_M | 240_M | 1.542 | 0.99 |
| 286_I | 290_F | 1.510 | 0.99 |
| 113_V | 117_T | 1.498 | 0.99 |
| 207_R | 210_Q | 1.492 | 0.99 |
| 133_S | 137_A | 1.491 | 0.99 |
| 63_L | 68_N | 1.472 | 0.99 |
| 233_T | 237_A | 1.440 | 0.98 |
| 266_P | 269_L | 1.435 | 0.98 |
| 150_C | 157_L | 1.429 | 0.98 |
| 151_I | 155_V | 1.412 | 0.98 |
| 184_W | 188_S | 1.406 | 0.98 |
| 57_V | 64_K | 1.402 | 0.98 |
| 129_D | 290_F | 1.390 | 0.98 |
| 212_A | 216_K | 1.364 | 0.98 |
| 201_M | 205_Y | 1.362 | 0.98 |
| 54_V | 76_T | 1.360 | 0.98 |
| 123_L | 201_M | 1.359 | 0.98 |
| 283_N | 287_Y | 1.337 | 0.97 |
| 143_R | 165_P | 1.306 | 0.97 |
| 241_G | 246_C | 1.301 | 0.97 |
| 209_Y | 233_T | 1.297 | 0.97 |
| 129_D | 231_R | 1.286 | 0.96 |
| 194_F | 197_P | 1.281 | 0.96 |
| 53_V | 129_D | 1.277 | 0.96 |
| 105_C | 177_C | 1.274 | 0.96 |
| 27_Y | 30_A | 1.265 | 0.96 |
| 79_I | 115_F | 1.264 | 0.96 |
| 35_I | 39_V | 1.263 | 0.96 |
| 250_F | 254_Y | 1.257 | 0.96 |
| 112_D | 116_C | 1.257 | 0.96 |
| 88_F | 104_W | 1.255 | 0.96 |
| 54_V | 72_V | 1.246 | 0.95 |
| 238_V | 290_F | 1.246 | 0.95 |
| 169_S | 172_S | 1.244 | 0.95 |
| 134_V | 138_V | 1.244 | 0.95 |
| 26_G | 29_L | 1.239 | 0.95 |
| 284_P | 287_Y | 1.238 | 0.95 |
| 118_S | 159_S | 1.238 | 0.95 |
| 101_G | 105_C | 1.236 | 0.95 |
| 144_T | 147_R | 1.232 | 0.95 |
| 70_F | 179_L | 1.225 | 0.95 |
| 81_V | 286_I | 1.222 | 0.95 |
| 165_P | 188_S | 1.221 | 0.95 |
| 190_S | 195_F | 1.214 | 0.94 |
| 252_F | 256_L | 1.211 | 0.94 |
| 196_A | 200_I | 1.210 | 0.94 |
| 112_D | 277_Y | 1.206 | 0.94 |
| 50_N | 53_V | 1.200 | 0.94 |
| 47_V | 79_I | 1.198 | 0.94 |
| 125_A | 151_I | 1.197 | 0.94 |
| 212_A | 241_G | 1.184 | 0.93 |
| 198_C | 244_V | 1.172 | 0.93 |
| 29_L | 32_I | 1.172 | 0.93 |
| 117_T | 274_W | 1.164 | 0.93 |
| 21_W | 24_D | 1.160 | 0.93 |
| 268_T | 272_F | 1.157 | 0.92 |
| 189_S | 193_S | 1.156 | 0.92 |
| 57_V | 63_L | 1.154 | 0.92 |
| 37_A | 41_F | 1.153 | 0.92 |
| 129_D | 143_R | 1.151 | 0.92 |
| 202_I | 206_I | 1.150 | 0.92 |
| 280_S | 283_N | 1.148 | 0.92 |
| 24_D | 28_S | 1.147 | 0.92 |
| 155_V | 245_V | 1.147 | 0.92 |
| 47_V | 289_I | 1.144 | 0.92 |
| 191_M | 196_A | 1.143 | 0.92 |
| 277_Y | 280_S | 1.140 | 0.92 |
| 28_S | 31_A | 1.140 | 0.92 |
| 139_Y | 144_T | 1.138 | 0.92 |
| 60_S | 63_L | 1.136 | 0.91 |
| 42_L | 278_C | 1.136 | 0.91 |
| 104_W | 114_L | 1.131 | 0.91 |
| 237_A | 248_F | 1.130 | 0.91 |
| 132_W | 151_I | 1.129 | 0.91 |
| 120_I | 250_F | 1.129 | 0.91 |
| 90_L | 160_A | 1.123 | 0.91 |
| 278_C | 282_L | 1.122 | 0.91 |
| 117_T | 193_S | 1.120 | 0.91 |
| 40_S | 44_L | 1.118 | 0.91 |
| 56_A | 60_S | 1.112 | 0.90 |
| 155_V | 250_F | 1.107 | 0.90 |
| 208_I | 236_L | 1.107 | 0.90 |
| 25_S | 29_L | 1.106 | 0.90 |
| 43_I | 90_L | 1.104 | 0.90 |
| 70_F | 148_V | 1.103 | 0.90 |
| 153_V | 245_V | 1.102 | 0.90 |
| 85_V | 116_C | 1.097 | 0.90 |
| 85_V | 274_W | 1.094 | 0.89 |
| 216_K | 219_I | 1.089 | 0.89 |
| 64_K | 95_M | 1.087 | 0.89 |
| 25_S | 30_A | 1.086 | 0.89 |
| 67_Q | 71_L | 1.080 | 0.89 |
| 138_V | 143_R | 1.076 | 0.88 |
| 112_D | 194_F | 1.071 | 0.88 |
| 248_F | 252_F | 1.070 | 0.88 |
| 240_M | 282_L | 1.063 | 0.87 |
| 194_F | 243_F | 1.054 | 0.87 |
| 194_F | 250_F | 1.054 | 0.87 |
| 23_A | 26_G | 1.051 | 0.87 |
| 26_G | 30_A | 1.051 | 0.87 |
| 210_Q | 214_T | 1.047 | 0.86 |
| 51_I | 55_I | 1.044 | 0.86 |
| 186_I | 190_S | 1.043 | 0.86 |
| 67_Q | 236_L | 1.043 | 0.86 |
| 54_V | 291_N | 1.043 | 0.86 |
| 166_P | 170_I | 1.042 | 0.86 |
| 221_E | 272_F | 1.040 | 0.86 |
| 247_W | 276_G | 1.035 | 0.86 |
| 73_S | 156_W | 1.033 | 0.85 |
| 152_I | 159_S | 1.025 | 0.85 |
| 129_D | 133_S | 1.023 | 0.85 |
| 88_F | 100_F | 1.018 | 0.84 |
| 222_K | 245_V | 1.015 | 0.84 |
| 60_S | 64_K | 1.014 | 0.84 |
| 82_A | 281_S | 1.014 | 0.84 |
| 108_Y | 112_D | 1.013 | 0.84 |
| 82_A | 135_T | 1.013 | 0.84 |
| 206_I | 210_Q | 1.012 | 0.84 |
| 122_H | 228_R | 1.011 | 0.84 |
| 112_D | 250_F | 1.006 | 0.83 |
| 121_V | 158_I | 1.004 | 0.83 |
| 24_D | 29_L | 1.001 | 0.83 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2rh1A | 1 | 0.8903 | 100 | 0.291 | Contact Map |
| 2z73A | 2 | 0.9806 | 100 | 0.33 | Contact Map |
| 3uonA | 1 | 0.8871 | 100 | 0.332 | Contact Map |
| 3pblA | 2 | 0.8677 | 100 | 0.334 | Contact Map |
| 4pxzA | 1 | 0.8613 | 100 | 0.336 | Contact Map |
| 3rzeA | 1 | 0.871 | 100 | 0.342 | Contact Map |
| 4u15A | 2 | 0.8871 | 100 | 0.343 | Contact Map |
| 3oduA | 2 | 0.929 | 100 | 0.344 | Contact Map |
| 4phuA | 1 | 0.8226 | 100 | 0.346 | Contact Map |
| 3vw7A | 1 | 0.8677 | 100 | 0.349 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)