| A2AR_LABOS_1e_40 |
| ID: | 1427838413 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 310 (282) | ||
| Sequences: | 7148 (4356.4) | ||
| Seq/Len: | 25.348 | ||
| Nf(neff/√len): | 259.4 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 25.348).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 46_T | 82_A | 5.009 | 1.00 |
| 203_L | 207_R | 3.232 | 1.00 |
| 131_Y | 136_Q | 2.692 | 1.00 |
| 98_W | 101_G | 2.691 | 1.00 |
| 103_V | 107_I | 2.653 | 1.00 |
| 102_K | 106_G | 2.572 | 1.00 |
| 73_S | 122_H | 2.570 | 1.00 |
| 125_A | 155_V | 2.558 | 1.00 |
| 260_C | 263_Y | 2.457 | 1.00 |
| 207_R | 210_Q | 2.304 | 1.00 |
| 110_A | 114_L | 2.266 | 1.00 |
| 242_V | 286_I | 2.092 | 1.00 |
| 73_S | 156_W | 2.021 | 1.00 |
| 57_V | 72_V | 2.014 | 1.00 |
| 129_D | 143_R | 1.982 | 1.00 |
| 33_A | 37_A | 1.929 | 1.00 |
| 125_A | 151_I | 1.857 | 1.00 |
| 69_L | 152_I | 1.823 | 1.00 |
| 150_C | 154_I | 1.821 | 1.00 |
| 205_Y | 208_I | 1.805 | 1.00 |
| 65_A | 68_N | 1.776 | 1.00 |
| 63_L | 68_N | 1.757 | 1.00 |
| 247_W | 276_G | 1.684 | 1.00 |
| 131_Y | 207_R | 1.676 | 1.00 |
| 51_I | 79_I | 1.674 | 1.00 |
| 149_K | 153_V | 1.666 | 1.00 |
| 164_S | 168_L | 1.645 | 1.00 |
| 122_H | 152_I | 1.627 | 1.00 |
| 206_I | 210_Q | 1.624 | 1.00 |
| 144_T | 147_R | 1.618 | 1.00 |
| 200_I | 204_V | 1.613 | 1.00 |
| 146_K | 150_C | 1.607 | 1.00 |
| 268_T | 272_F | 1.596 | 1.00 |
| 72_V | 76_T | 1.587 | 1.00 |
| 202_I | 206_I | 1.582 | 1.00 |
| 131_Y | 135_T | 1.559 | 1.00 |
| 54_V | 72_V | 1.552 | 1.00 |
| 166_P | 169_S | 1.537 | 1.00 |
| 75_A | 78_D | 1.513 | 1.00 |
| 201_M | 205_Y | 1.505 | 1.00 |
| 235_V | 290_F | 1.502 | 1.00 |
| 91_A | 100_F | 1.492 | 1.00 |
| 46_T | 281_S | 1.492 | 1.00 |
| 186_I | 189_S | 1.454 | 1.00 |
| 151_I | 155_V | 1.453 | 1.00 |
| 47_V | 79_I | 1.442 | 1.00 |
| 283_N | 287_Y | 1.431 | 1.00 |
| 51_I | 55_I | 1.430 | 1.00 |
| 188_S | 192_A | 1.423 | 1.00 |
| 184_W | 188_S | 1.418 | 1.00 |
| 194_F | 248_F | 1.417 | 1.00 |
| 110_A | 167_L | 1.415 | 1.00 |
| 47_V | 82_A | 1.407 | 0.99 |
| 194_F | 198_C | 1.401 | 0.99 |
| 114_L | 160_A | 1.391 | 0.99 |
| 88_F | 104_W | 1.377 | 0.99 |
| 105_C | 177_C | 1.376 | 0.99 |
| 198_C | 244_V | 1.369 | 0.99 |
| 215_R | 219_I | 1.350 | 0.99 |
| 123_L | 201_M | 1.335 | 0.99 |
| 207_R | 211_V | 1.310 | 0.99 |
| 244_V | 248_F | 1.308 | 0.99 |
| 237_A | 241_G | 1.305 | 0.99 |
| 118_S | 159_S | 1.275 | 0.99 |
| 250_F | 254_Y | 1.273 | 0.99 |
| 248_F | 252_F | 1.272 | 0.99 |
| 60_S | 297_A | 1.267 | 0.99 |
| 101_G | 104_W | 1.255 | 0.99 |
| 232_F | 235_V | 1.250 | 0.99 |
| 252_F | 256_L | 1.249 | 0.99 |
| 104_W | 107_I | 1.240 | 0.98 |
| 43_I | 90_L | 1.236 | 0.98 |
| 190_S | 255_S | 1.234 | 0.98 |
| 278_C | 282_L | 1.231 | 0.98 |
| 133_S | 137_A | 1.231 | 0.98 |
| 208_I | 212_A | 1.227 | 0.98 |
| 133_S | 138_V | 1.221 | 0.98 |
| 118_S | 122_H | 1.198 | 0.98 |
| 121_V | 158_I | 1.187 | 0.98 |
| 131_Y | 203_L | 1.186 | 0.98 |
| 69_L | 149_K | 1.172 | 0.98 |
| 111_L | 115_F | 1.167 | 0.97 |
| 22_S | 263_Y | 1.159 | 0.97 |
| 146_K | 149_K | 1.157 | 0.97 |
| 66_P | 148_V | 1.156 | 0.97 |
| 129_D | 133_S | 1.154 | 0.97 |
| 54_V | 79_I | 1.147 | 0.97 |
| 250_F | 276_G | 1.147 | 0.97 |
| 172_S | 175_P | 1.144 | 0.97 |
| 36_A | 90_L | 1.138 | 0.97 |
| 53_V | 288_T | 1.135 | 0.97 |
| 209_Y | 212_A | 1.134 | 0.97 |
| 190_S | 195_F | 1.131 | 0.97 |
| 53_V | 57_V | 1.131 | 0.97 |
| 154_I | 158_I | 1.129 | 0.97 |
| 238_V | 286_I | 1.128 | 0.97 |
| 273_F | 277_Y | 1.126 | 0.97 |
| 101_G | 105_C | 1.118 | 0.96 |
| 132_W | 142_K | 1.118 | 0.96 |
| 54_V | 75_A | 1.116 | 0.96 |
| 77_A | 118_S | 1.115 | 0.96 |
| 135_T | 211_V | 1.115 | 0.96 |
| 54_V | 57_V | 1.112 | 0.96 |
| 127_S | 236_L | 1.111 | 0.96 |
| 77_A | 119_S | 1.104 | 0.96 |
| 169_S | 172_S | 1.101 | 0.96 |
| 66_P | 70_F | 1.092 | 0.96 |
| 35_I | 39_V | 1.090 | 0.96 |
| 273_F | 276_G | 1.086 | 0.96 |
| 110_A | 160_A | 1.086 | 0.96 |
| 88_F | 108_Y | 1.085 | 0.96 |
| 24_D | 27_Y | 1.083 | 0.96 |
| 117_T | 193_S | 1.082 | 0.96 |
| 57_V | 63_L | 1.082 | 0.96 |
| 85_V | 89_S | 1.080 | 0.95 |
| 296_R | 299_Q | 1.078 | 0.95 |
| 299_Q | 303_C | 1.075 | 0.95 |
| 251_F | 254_Y | 1.075 | 0.95 |
| 297_A | 300_K | 1.072 | 0.95 |
| 112_D | 120_I | 1.072 | 0.95 |
| 126_I | 129_D | 1.071 | 0.95 |
| 201_M | 240_M | 1.070 | 0.95 |
| 91_A | 95_M | 1.066 | 0.95 |
| 70_F | 148_V | 1.065 | 0.95 |
| 198_C | 248_F | 1.064 | 0.95 |
| 209_Y | 213_K | 1.061 | 0.95 |
| 278_C | 281_S | 1.059 | 0.95 |
| 209_Y | 233_T | 1.047 | 0.94 |
| 194_F | 243_F | 1.045 | 0.94 |
| 81_V | 86_M | 1.037 | 0.94 |
| 233_T | 239_V | 1.035 | 0.94 |
| 128_L | 204_V | 1.029 | 0.94 |
| 186_I | 190_S | 1.029 | 0.94 |
| 187_L | 191_M | 1.025 | 0.93 |
| 58_L | 72_V | 1.025 | 0.93 |
| 113_V | 163_S | 1.023 | 0.93 |
| 121_V | 159_S | 1.020 | 0.93 |
| 238_V | 290_F | 1.017 | 0.93 |
| 53_V | 283_N | 1.007 | 0.93 |
| 95_M | 99_Y | 1.007 | 0.93 |
| 164_S | 167_L | 1.006 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2rh1A | 1 | 0.8903 | 100 | 0.259 | Contact Map |
| 3uonA | 1 | 0.8871 | 100 | 0.295 | Contact Map |
| 4pxzA | 1 | 0.8613 | 100 | 0.3 | Contact Map |
| 3pblA | 2 | 0.8677 | 100 | 0.301 | Contact Map |
| 2z73A | 2 | 0.9806 | 100 | 0.304 | Contact Map |
| 3oduA | 2 | 0.929 | 100 | 0.31 | Contact Map |
| 4eiyA | 1 | 0.9032 | 100 | 0.314 | Contact Map |
| 4u15A | 2 | 0.8871 | 100 | 0.318 | Contact Map |
| 3vw7A | 1 | 0.8677 | 100 | 0.319 | Contact Map |
| 3rzeA | 1 | 0.8742 | 100 | 0.32 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)