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OPENSEQ.org

BSU27600 SpoT

ID: 1422435391 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 50% gaps before running GREMLIN.
Length: 734 (713)
Sequences: 1025 (904.3)
Seq/Len: 1.438
Nf(neff/√len): 33.9

HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.438).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 50% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
91_K96_E4.1751.00
417_Y425_N3.9941.00
433_N447_D3.7661.00
80_V87_L3.0931.00
66_M139_L3.0901.00
432_V437_V2.7971.00
274_S277_D2.7921.00
228_R231_E2.7811.00
413_I467_L2.7661.00
468_A477_I2.7631.00
30_K93_A2.6641.00
150_R186_E2.6511.00
490_V534_A2.6481.00
717_V729_V2.6451.00
237_D330_F2.6001.00
663_I720_I2.4991.00
235_K273_N2.4601.00
423_I453_T2.3861.00
271_L330_F2.3771.00
668_R674_E2.3410.99
443_L449_V2.3270.99
497_V536_V2.2460.99
28_V71_I2.1970.99
69_S97_E2.1870.99
80_V90_L2.1560.99
157_Q160_Q2.1020.99
273_N330_F2.0880.98
225_V285_I2.0530.98
239_S333_H2.0410.98
257_K262_I2.0230.98
398_T404_I2.0040.98
217_Y317_G1.9450.97
442_K469_Q1.9060.97
61_L70_T1.8910.97
470_T473_A1.8740.96
705_I716_V1.8700.96
27_F72_A1.8350.96
66_M70_T1.8340.96
206_L259_F1.8050.95
196_N199_Q1.8010.95
45_K155_L1.7850.95
526_F535_A1.7850.95
665_G671_L1.7840.95
152_L163_I1.7610.94
661_I717_V1.7340.94
689_V703_M1.7310.94
203_I245_I1.7180.93
34_Y85_V1.7120.93
152_L194_Y1.6810.92
668_R671_L1.6740.92
87_L103_D1.6720.92
207_M245_I1.6680.92
15_K63_D1.6590.92
58_A71_I1.6530.92
82_D148_N1.6450.91
78_D82_D1.6240.91
82_D107_K1.6060.90
272_V281_V1.6030.90
165_N169_E1.6020.90
616_T622_S1.5940.90
528_N531_D1.5650.88
81_E148_N1.5580.88
148_N151_T1.5560.88
660_E704_A1.5520.88
152_L164_S1.5480.88
243_K251_K1.5270.87
54_P148_N1.5220.86
432_V441_H1.5190.86
215_E219_D1.5170.86
431_K450_E1.5100.86
273_N277_D1.5060.86
78_D81_E1.4990.85
35_A51_I1.4960.85
16_A28_V1.4920.85
78_D148_N1.4890.85
149_M187_L1.4830.84
44_R82_D1.4760.84
441_H444_R1.4690.84
44_R151_T1.4640.83
107_K148_N1.4640.83
679_V686_I1.4590.83
226_K230_E1.4530.83
29_E33_L1.4500.83
89_D92_E1.4480.82
147_H151_T1.4430.82
276_K280_A1.4400.82
486_R531_D1.4400.82
164_S191_A1.4290.81
81_E151_T1.4280.81
421_S425_N1.4230.81
144_D148_N1.4200.81
343_H346_Y1.4200.81
82_D151_T1.4180.81
412_P430_A1.4170.81
81_E106_T1.4100.80
684_T712_H1.4080.80
39_H78_D1.4050.80
44_R50_Y1.4040.80
168_L246_Y1.4030.80
218_V240_G1.3960.79
612_V631_V1.3920.79
39_H148_N1.3890.79
603_C628_C1.3890.79
410_S720_I1.3890.79
77_H81_E1.3880.79
82_D106_T1.3870.79
39_H53_H1.3790.78
160_Q194_Y1.3770.78
39_H81_E1.3660.77
220_E319_K1.3620.77
157_Q161_R1.3610.77
251_K324_E1.3600.77
44_R148_N1.3560.76
204_V245_I1.3520.76
295_R314_T1.3510.76
286_H313_T1.3480.76
44_R47_G1.3480.76
281_V284_I1.3470.76
202_R206_L1.3400.75
53_H147_H1.3360.75
39_H50_Y1.3350.75
85_V89_D1.3300.74
100_M133_Q1.3300.74
670_G685_N1.3270.74
53_H77_H1.3260.74
27_F69_S1.3240.74
462_Q481_F1.3190.73
104_G137_V1.3150.73
679_V716_V1.3120.73
82_D144_D1.3100.73
39_H82_D1.3050.72
61_L71_I1.3030.72
246_Y250_R1.3020.72
624_H628_C1.3020.72
104_G126_K1.3010.72
54_P81_E1.3000.72
87_L100_M1.2980.72
227_K231_E1.2980.72
107_K144_D1.2890.71
44_R81_E1.2870.71
33_L36_E1.2870.71
671_L674_E1.2850.71
53_H78_D1.2850.71
26_A30_K1.2840.71
78_D83_T1.2840.71
39_H77_H1.2800.70
77_H148_N1.2790.70
277_D280_A1.2780.70
54_P82_D1.2780.70
679_V705_I1.2770.70
667_D699_A1.2750.70
613_G641_I1.2740.70
243_K343_H1.2720.70
87_L91_K1.2700.69
39_H42_Q1.2700.69
587_V611_I1.2690.69
82_D147_H1.2680.69
149_M152_L1.2650.69
427_T439_L1.2640.69
210_K213_E1.2610.69
39_H44_R1.2600.69
57_V144_D1.2600.69
53_H148_N1.2600.69
251_K343_H1.2570.68
58_A74_G1.2560.68
156_P159_K1.2510.68
155_L159_K1.2470.67
44_R77_H1.2470.67
81_E147_H1.2460.67
228_R288_C1.2440.67
44_R78_D1.2440.67
50_Y78_D1.2420.67
222_V226_K1.2400.67
603_C624_H1.2400.67
217_Y318_P1.2390.67
44_R147_H1.2390.67
602_C628_C1.2390.67
330_F334_E1.2370.66
68_P72_A1.2350.66
614_F639_R1.2340.66
209_K321_D1.2310.66
279_Y286_H1.2310.66
44_R107_K1.2280.66
77_H147_H1.2220.65
211_R242_P1.2190.65
54_P78_D1.2170.65
27_F30_K1.2160.65
47_G53_H1.2130.64
78_D106_T1.2120.64
518_Q522_D1.2110.64
341_A347_K1.2110.64
155_L163_I1.2080.64
78_D107_K1.2080.64
412_P432_V1.2070.64
48_E53_H1.2060.64
283_G286_H1.2050.63
490_V530_E1.2030.63
663_I703_M1.2000.63
81_E144_D1.1980.63
39_H54_P1.1970.63
185_W244_H1.1960.63
54_P107_K1.1950.63
50_Y148_N1.1900.62
185_W211_R1.1890.62
50_Y107_K1.1870.62
671_L723_I1.1850.62
71_I74_G1.1830.61
54_P77_H1.1820.61
106_T148_N1.1820.61
418_R472_Q1.1810.61
333_H337_E1.1810.61
78_D151_T1.1810.61
366_R369_L1.1800.61
343_H347_K1.1780.61
199_Q202_R1.1780.61
77_H82_D1.1770.61
77_H151_T1.1750.61
83_T148_N1.1750.61
610_D626_E1.1750.61
623_V641_I1.1740.61
668_R699_A1.1740.61
267_A343_H1.1710.60
77_H144_D1.1660.60
60_I150_R1.1660.60
61_L74_G1.1650.60
39_H106_T1.1650.60
50_Y77_H1.1640.60
44_R54_P1.1620.59
54_P147_H1.1610.59
31_A35_A1.1610.59
86_T89_D1.1610.59
285_I315_V1.1590.59
78_D147_H1.1570.59
53_H82_D1.1540.59
590_K644_E1.1530.58
616_T630_N1.1520.58
341_A346_Y1.1520.58
106_T144_D1.1490.58
61_L105_V1.1470.58
39_H107_K1.1470.58
324_E343_H1.1400.57
107_K151_T1.1400.57
279_Y283_G1.1350.57
50_Y82_D1.1330.56
83_T151_T1.1330.56
396_V443_L1.1320.56
44_R53_H1.1280.56
87_L99_A1.1270.56
269_R347_K1.1240.56
16_A21_S1.1230.55
223_N227_K1.1230.55
201_Y204_V1.1220.55
44_R144_D1.1210.55
39_H151_T1.1200.55
229_V281_V1.1180.55
605_P628_C1.1160.55
53_H151_T1.1150.55
521_A529_E1.1140.55
10_E14_D1.1120.54
83_T86_T1.1110.54
219_D222_V1.1100.54
50_Y147_H1.1100.54
50_Y53_H1.1090.54
82_D159_K1.1070.54
39_H147_H1.1050.54
221_V323_L1.1040.53
77_H106_T1.1030.53
602_C624_H1.1030.53
623_V652_Q1.1020.53
38_A89_D1.0970.53
271_L439_L1.0940.52
47_G148_N1.0920.52
203_I259_F1.0910.52
378_A721_K1.0890.52
81_E159_K1.0880.52
144_D151_T1.0880.52
149_M167_T1.0870.52
80_V106_T1.0870.52
60_I186_E1.0830.51
243_K347_K1.0820.51
243_K324_E1.0820.51
53_H57_V1.0820.51
107_K147_H1.0820.51
91_K99_A1.0820.51
47_G77_H1.0800.51
78_D167_T1.0790.51
103_D126_K1.0770.51
83_T107_K1.0740.50
131_M135_I1.0740.50
80_V103_D1.0730.50
668_R723_I1.0730.50
623_V643_V1.0710.50
691_G701_I1.0690.50
53_H81_E1.0670.50
39_H47_G1.0620.49
39_H83_T1.0610.49
279_Y282_L1.0610.49
616_T639_R1.0600.49
40_R270_I1.0580.49
72_A96_E1.0580.49
367_E371_F1.0580.49
340_V410_S1.0560.49
526_F532_M1.0560.49
81_E141_K1.0550.48
54_P151_T1.0540.48
27_F98_V1.0540.48
144_D147_H1.0530.48
226_K237_D1.0510.48
225_V325_V1.0510.48
671_L725_D1.0500.48
668_R725_D1.0490.48
60_I145_R1.0490.48
243_K267_A1.0490.48
634_N640_L1.0480.48
127_M291_P1.0480.48
251_K269_R1.0470.48
249_Y253_V1.0470.48
54_P83_T1.0470.48
243_K341_A1.0460.48
42_Q82_D1.0460.48
421_S476_K1.0440.47
39_H159_K1.0440.47
106_T147_H1.0430.47
47_G144_D1.0430.47
101_L140_I1.0420.47
494_R533_Y1.0420.47
431_K436_M1.0410.47
269_R341_A1.0410.47
161_R325_V1.0400.47
251_K341_A1.0390.47
324_E341_A1.0390.47
50_Y151_T1.0380.47
192_L199_Q1.0370.47
592_I595_L1.0370.47
309_Q335_I1.0370.47
77_H107_K1.0360.47
57_V82_D1.0360.47
207_M265_L1.0350.46
146_L183_I1.0340.46
324_E347_K1.0340.46
13_I25_I1.0320.46
159_K163_I1.0300.46
42_Q148_N1.0290.46
34_Y413_I1.0260.46
269_R326_Q1.0240.45
19_Y65_E1.0230.45
251_K326_Q1.0230.45
535_A541_I1.0220.45
78_D144_D1.0220.45
484_Q487_E1.0220.45
409_G442_K1.0210.45
717_V721_K1.0210.45
34_Y93_A1.0200.45
233_N449_V1.0200.45
269_R346_Y1.0200.45
269_R343_H1.0200.45
520_V546_V1.0190.45
44_R106_T1.0180.45
486_R490_V1.0180.45
73_G101_L1.0170.45
44_R83_T1.0160.45
251_K267_A1.0110.44
88_D449_V1.0110.44
282_L286_H1.0110.44
672_L689_V1.0090.44
669_R685_N1.0070.44
386_K413_I1.0070.44
62_V192_L1.0060.44
76_L98_V1.0060.44
286_H296_F1.0050.43
269_R324_E1.0050.43
386_K390_F1.0050.43
687_S706_F1.0010.43
602_C605_P1.0010.43
46_S82_D1.0010.43
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Sep14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1vj7A20.45641000.521Contact Map
3l9dA20.25481000.798Contact Map
2be3A20.26161000.833Contact Map
3nqwA20.23711000.845Contact Map
3nr1A20.2331000.848Contact Map
2ekiA10.106399.90.917Contact Map
4ce4c10.324399.50.943Contact Map
3hvzA60.087299.50.944Contact Map
1wxqA10.404699.50.945Contact Map
1wwtA10.118599.30.951Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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