| MalT |
| ID: | 1417088089 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 806 (765) | ||
| Sequences: | 2938 (2610.6) | ||
| Seq/Len: | 3.841 | ||
| Nf(neff/√len): | 94.4 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.841).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 665_D | 668_A | 5.950 | 1.00 |
| 585_R | 588_E | 5.938 | 1.00 |
| 624_D | 627_N | 5.801 | 1.00 |
| 543_F | 546_T | 5.253 | 1.00 |
| 503_E | 506_R | 4.779 | 1.00 |
| 703_E | 706_P | 4.621 | 1.00 |
| 49_I | 158_L | 4.524 | 1.00 |
| 464_N | 467_E | 4.301 | 1.00 |
| 424_R | 427_E | 4.273 | 1.00 |
| 743_R | 746_D | 3.914 | 1.00 |
| 344_H | 363_H | 3.312 | 1.00 |
| 277_L | 347_A | 3.043 | 1.00 |
| 535_S | 551_Q | 2.805 | 1.00 |
| 47_T | 51_Q | 2.801 | 1.00 |
| 191_A | 222_L | 2.770 | 1.00 |
| 130_Y | 157_V | 2.645 | 1.00 |
| 548_W | 552_E | 2.341 | 1.00 |
| 519_R | 553_K | 2.327 | 1.00 |
| 188_H | 209_S | 2.307 | 1.00 |
| 53_A | 126_V | 2.303 | 1.00 |
| 37_I | 49_I | 2.291 | 1.00 |
| 264_D | 300_E | 2.284 | 1.00 |
| 538_L | 543_F | 2.280 | 1.00 |
| 191_A | 212_C | 2.230 | 1.00 |
| 552_E | 576_R | 2.205 | 1.00 |
| 495_G | 511_M | 2.205 | 1.00 |
| 187_T | 190_E | 2.186 | 1.00 |
| 590_E | 622_R | 2.186 | 1.00 |
| 508_L | 541_Q | 2.154 | 1.00 |
| 348_A | 364_A | 2.129 | 1.00 |
| 39_S | 43_Y | 2.117 | 1.00 |
| 548_W | 583_W | 2.115 | 1.00 |
| 548_W | 576_R | 2.108 | 1.00 |
| 211_I | 222_L | 2.108 | 1.00 |
| 274_S | 320_Y | 2.106 | 1.00 |
| 538_L | 546_T | 2.056 | 1.00 |
| 26_L | 52_W | 2.045 | 1.00 |
| 429_N | 433_A | 2.029 | 1.00 |
| 616_I | 632_L | 2.008 | 1.00 |
| 195_F | 226_A | 2.006 | 1.00 |
| 712_E | 734_L | 2.001 | 1.00 |
| 619_S | 624_D | 1.989 | 1.00 |
| 267_T | 300_E | 1.971 | 1.00 |
| 49_I | 126_V | 1.969 | 1.00 |
| 601_S | 634_R | 1.962 | 1.00 |
| 590_E | 594_R | 1.952 | 1.00 |
| 629_R | 633_N | 1.942 | 1.00 |
| 275_A | 329_R | 1.934 | 1.00 |
| 734_L | 738_Y | 1.926 | 1.00 |
| 267_T | 301_I | 1.911 | 1.00 |
| 128_D | 158_L | 1.899 | 1.00 |
| 636_E | 656_R | 1.881 | 1.00 |
| 629_R | 663_T | 1.874 | 1.00 |
| 619_S | 627_N | 1.859 | 1.00 |
| 20_E | 190_E | 1.797 | 0.99 |
| 512_Q | 534_Q | 1.783 | 0.99 |
| 329_R | 340_L | 1.759 | 0.99 |
| 731_L | 753_D | 1.759 | 0.99 |
| 27_S | 52_W | 1.750 | 0.99 |
| 594_R | 615_L | 1.741 | 0.99 |
| 698_Q | 706_P | 1.731 | 0.99 |
| 576_R | 592_S | 1.705 | 0.99 |
| 660_W | 665_D | 1.701 | 0.99 |
| 389_E | 392_L | 1.660 | 0.99 |
| 587_D | 622_R | 1.646 | 0.99 |
| 125_L | 155_L | 1.643 | 0.99 |
| 577_A | 593_A | 1.637 | 0.99 |
| 272_L | 339_E | 1.635 | 0.99 |
| 51_Q | 54_A | 1.631 | 0.99 |
| 41_A | 160_R | 1.629 | 0.99 |
| 633_N | 656_R | 1.627 | 0.99 |
| 160_R | 321_H | 1.618 | 0.99 |
| 559_N | 595_S | 1.611 | 0.99 |
| 123_L | 153_L | 1.603 | 0.98 |
| 325_G | 329_R | 1.601 | 0.98 |
| 532_I | 575_I | 1.600 | 0.98 |
| 660_W | 668_A | 1.598 | 0.98 |
| 419_M | 427_E | 1.594 | 0.98 |
| 264_D | 267_T | 1.591 | 0.98 |
| 719_R | 753_D | 1.590 | 0.98 |
| 531_L | 553_K | 1.570 | 0.98 |
| 580_L | 588_E | 1.567 | 0.98 |
| 575_I | 614_M | 1.566 | 0.98 |
| 26_L | 35_A | 1.566 | 0.98 |
| 268_R | 272_L | 1.564 | 0.98 |
| 590_E | 615_L | 1.557 | 0.98 |
| 629_R | 656_R | 1.552 | 0.98 |
| 580_L | 592_S | 1.543 | 0.98 |
| 508_L | 534_Q | 1.539 | 0.98 |
| 738_Y | 746_D | 1.536 | 0.98 |
| 735_N | 751_L | 1.534 | 0.98 |
| 16_T | 47_T | 1.533 | 0.98 |
| 519_R | 527_A | 1.533 | 0.98 |
| 555_F | 569_H | 1.512 | 0.98 |
| 708_E | 743_R | 1.509 | 0.97 |
| 508_L | 512_Q | 1.508 | 0.97 |
| 186_F | 194_F | 1.504 | 0.97 |
| 16_T | 19_R | 1.504 | 0.97 |
| 345_R | 349_E | 1.498 | 0.97 |
| 664_G | 669_A | 1.498 | 0.97 |
| 619_S | 631_Q | 1.488 | 0.97 |
| 708_E | 737_L | 1.487 | 0.97 |
| 715_N | 730_N | 1.483 | 0.97 |
| 531_L | 557_L | 1.479 | 0.97 |
| 276_I | 343_I | 1.476 | 0.97 |
| 697_A | 701_L | 1.463 | 0.97 |
| 748_Q | 752_L | 1.457 | 0.97 |
| 276_I | 329_R | 1.452 | 0.97 |
| 580_L | 585_R | 1.452 | 0.97 |
| 273_K | 347_A | 1.450 | 0.97 |
| 765_S | 769_I | 1.440 | 0.96 |
| 352_M | 372_M | 1.439 | 0.96 |
| 75_A | 78_L | 1.439 | 0.96 |
| 702_G | 707_A | 1.437 | 0.96 |
| 50_S | 61_W | 1.434 | 0.96 |
| 435_A | 438_E | 1.433 | 0.96 |
| 544_L | 579_L | 1.424 | 0.96 |
| 579_L | 583_W | 1.418 | 0.96 |
| 589_A | 618_C | 1.406 | 0.96 |
| 22_L | 48_L | 1.395 | 0.95 |
| 520_Q | 560_E | 1.393 | 0.95 |
| 587_D | 667_A | 1.390 | 0.95 |
| 545_Q | 583_W | 1.387 | 0.95 |
| 587_D | 626_D | 1.386 | 0.95 |
| 49_I | 53_A | 1.378 | 0.95 |
| 195_F | 208_S | 1.376 | 0.95 |
| 128_D | 131_H | 1.375 | 0.95 |
| 627_N | 631_Q | 1.373 | 0.95 |
| 557_L | 638_L | 1.367 | 0.95 |
| 728_N | 758_A | 1.366 | 0.95 |
| 329_R | 344_H | 1.364 | 0.95 |
| 548_W | 579_L | 1.356 | 0.94 |
| 505_T | 545_Q | 1.352 | 0.94 |
| 520_Q | 641_N | 1.348 | 0.94 |
| 738_Y | 750_V | 1.347 | 0.94 |
| 708_E | 712_E | 1.343 | 0.94 |
| 538_L | 550_T | 1.338 | 0.94 |
| 332_C | 336_L | 1.337 | 0.94 |
| 576_R | 580_L | 1.334 | 0.94 |
| 342_E | 346_A | 1.332 | 0.94 |
| 708_E | 734_L | 1.329 | 0.94 |
| 171_R | 178_E | 1.329 | 0.94 |
| 615_L | 631_Q | 1.326 | 0.94 |
| 58_D | 121_S | 1.325 | 0.94 |
| 53_A | 124_Y | 1.323 | 0.93 |
| 531_L | 550_T | 1.320 | 0.93 |
| 537_I | 541_Q | 1.315 | 0.93 |
| 494_L | 498_L | 1.314 | 0.93 |
| 260_L | 327_F | 1.313 | 0.93 |
| 551_Q | 575_I | 1.312 | 0.93 |
| 130_Y | 139_H | 1.312 | 0.93 |
| 218_W | 221_A | 1.309 | 0.93 |
| 18_V | 22_L | 1.305 | 0.93 |
| 40_P | 43_Y | 1.303 | 0.93 |
| 599_V | 638_L | 1.301 | 0.93 |
| 528_L | 554_A | 1.300 | 0.93 |
| 257_D | 327_F | 1.298 | 0.93 |
| 735_N | 750_V | 1.297 | 0.93 |
| 210_R | 214_D | 1.295 | 0.92 |
| 495_G | 514_T | 1.295 | 0.92 |
| 708_E | 741_A | 1.295 | 0.92 |
| 612_L | 631_Q | 1.290 | 0.92 |
| 731_L | 750_V | 1.289 | 0.92 |
| 619_S | 622_R | 1.288 | 0.92 |
| 633_N | 637_N | 1.287 | 0.92 |
| 555_F | 576_R | 1.286 | 0.92 |
| 124_Y | 156_V | 1.284 | 0.92 |
| 535_S | 550_T | 1.283 | 0.92 |
| 556_Q | 637_N | 1.278 | 0.92 |
| 252_S | 330_Q | 1.272 | 0.92 |
| 469_E | 494_L | 1.271 | 0.92 |
| 752_L | 756_K | 1.269 | 0.91 |
| 476_L | 494_L | 1.259 | 0.91 |
| 480_P | 487_R | 1.259 | 0.91 |
| 548_W | 585_R | 1.258 | 0.91 |
| 670_A | 674_R | 1.256 | 0.91 |
| 755_L | 759_N | 1.254 | 0.91 |
| 544_L | 586_L | 1.254 | 0.91 |
| 128_D | 322_P | 1.253 | 0.91 |
| 718_A | 730_N | 1.251 | 0.91 |
| 369_D | 372_M | 1.249 | 0.91 |
| 715_N | 734_L | 1.246 | 0.90 |
| 16_T | 51_Q | 1.242 | 0.90 |
| 188_H | 192_K | 1.241 | 0.90 |
| 204_E | 207_E | 1.238 | 0.90 |
| 629_R | 660_W | 1.235 | 0.90 |
| 738_Y | 743_R | 1.229 | 0.90 |
| 18_V | 23_L | 1.226 | 0.90 |
| 516_Q | 556_Q | 1.223 | 0.89 |
| 670_A | 701_L | 1.222 | 0.89 |
| 695_A | 714_L | 1.218 | 0.89 |
| 731_L | 757_L | 1.216 | 0.89 |
| 728_N | 754_A | 1.214 | 0.89 |
| 598_E | 637_N | 1.214 | 0.89 |
| 57_N | 124_Y | 1.213 | 0.89 |
| 191_A | 215_V | 1.213 | 0.89 |
| 705_E | 741_A | 1.212 | 0.89 |
| 469_E | 473_K | 1.210 | 0.89 |
| 474_L | 595_S | 1.209 | 0.89 |
| 519_R | 531_L | 1.206 | 0.88 |
| 699_I | 737_L | 1.203 | 0.88 |
| 560_E | 641_N | 1.197 | 0.88 |
| 36_L | 159_S | 1.195 | 0.88 |
| 727_L | 731_L | 1.194 | 0.88 |
| 624_D | 665_D | 1.192 | 0.88 |
| 590_E | 619_S | 1.191 | 0.88 |
| 698_Q | 703_E | 1.190 | 0.88 |
| 517_M | 557_L | 1.189 | 0.88 |
| 728_N | 757_L | 1.189 | 0.88 |
| 464_N | 543_F | 1.189 | 0.88 |
| 746_D | 750_V | 1.188 | 0.87 |
| 19_R | 22_L | 1.185 | 0.87 |
| 36_L | 130_Y | 1.184 | 0.87 |
| 590_E | 618_C | 1.178 | 0.87 |
| 177_L | 181_S | 1.177 | 0.87 |
| 134_T | 137_V | 1.175 | 0.87 |
| 416_A | 432_L | 1.174 | 0.87 |
| 415_Q | 419_M | 1.171 | 0.86 |
| 144_F | 148_H | 1.171 | 0.86 |
| 444_E | 447_M | 1.170 | 0.86 |
| 735_N | 754_A | 1.166 | 0.86 |
| 535_S | 542_G | 1.160 | 0.86 |
| 280_M | 320_Y | 1.160 | 0.86 |
| 192_K | 208_S | 1.158 | 0.86 |
| 212_C | 215_V | 1.158 | 0.86 |
| 666_K | 669_A | 1.154 | 0.85 |
| 559_N | 601_S | 1.153 | 0.85 |
| 698_Q | 738_Y | 1.150 | 0.85 |
| 517_M | 638_L | 1.148 | 0.85 |
| 456_A | 468_A | 1.145 | 0.85 |
| 520_Q | 602_S | 1.144 | 0.85 |
| 269_H | 339_E | 1.144 | 0.85 |
| 306_L | 324_F | 1.143 | 0.85 |
| 498_L | 619_S | 1.142 | 0.85 |
| 23_L | 52_W | 1.142 | 0.85 |
| 494_L | 510_L | 1.140 | 0.84 |
| 270_F | 274_S | 1.139 | 0.84 |
| 511_M | 530_S | 1.136 | 0.84 |
| 224_L | 259_V | 1.136 | 0.84 |
| 474_L | 477_E | 1.132 | 0.84 |
| 747_A | 754_A | 1.130 | 0.84 |
| 456_A | 472_A | 1.130 | 0.84 |
| 22_L | 190_E | 1.129 | 0.84 |
| 41_A | 131_H | 1.128 | 0.84 |
| 469_E | 629_R | 1.127 | 0.83 |
| 626_D | 663_T | 1.123 | 0.83 |
| 583_W | 622_R | 1.120 | 0.83 |
| 573_V | 592_S | 1.117 | 0.83 |
| 616_I | 695_A | 1.116 | 0.83 |
| 190_E | 193_Q | 1.116 | 0.83 |
| 625_L | 666_K | 1.116 | 0.83 |
| 528_L | 609_L | 1.115 | 0.83 |
| 552_E | 588_E | 1.115 | 0.83 |
| 670_A | 703_E | 1.113 | 0.82 |
| 553_K | 595_S | 1.113 | 0.82 |
| 498_L | 503_E | 1.111 | 0.82 |
| 267_T | 304_Q | 1.109 | 0.82 |
| 556_Q | 598_E | 1.106 | 0.82 |
| 322_P | 326_N | 1.104 | 0.82 |
| 746_D | 749_R | 1.102 | 0.82 |
| 131_H | 134_T | 1.102 | 0.82 |
| 737_L | 741_A | 1.101 | 0.81 |
| 640_G | 675_H | 1.098 | 0.81 |
| 220_T | 321_H | 1.096 | 0.81 |
| 512_Q | 594_R | 1.096 | 0.81 |
| 511_M | 534_Q | 1.096 | 0.81 |
| 43_Y | 218_W | 1.092 | 0.81 |
| 503_E | 585_R | 1.092 | 0.81 |
| 625_L | 663_T | 1.090 | 0.81 |
| 519_R | 559_N | 1.089 | 0.80 |
| 618_C | 622_R | 1.087 | 0.80 |
| 633_N | 712_E | 1.084 | 0.80 |
| 732_L | 758_A | 1.081 | 0.80 |
| 513_Q | 517_M | 1.079 | 0.80 |
| 216_S | 322_P | 1.078 | 0.80 |
| 196_D | 208_S | 1.078 | 0.80 |
| 580_L | 619_S | 1.077 | 0.79 |
| 129_D | 305_G | 1.077 | 0.79 |
| 591_A | 671_N | 1.077 | 0.79 |
| 191_A | 217_G | 1.076 | 0.79 |
| 364_A | 372_M | 1.076 | 0.79 |
| 626_D | 667_A | 1.076 | 0.79 |
| 554_A | 558_I | 1.075 | 0.79 |
| 188_H | 213_D | 1.074 | 0.79 |
| 630_S | 671_N | 1.074 | 0.79 |
| 516_Q | 598_E | 1.070 | 0.79 |
| 711_L | 737_L | 1.070 | 0.79 |
| 39_S | 45_K | 1.068 | 0.79 |
| 719_R | 722_R | 1.066 | 0.78 |
| 47_T | 223_Q | 1.064 | 0.78 |
| 149_Q | 153_L | 1.059 | 0.78 |
| 224_L | 227_L | 1.057 | 0.78 |
| 508_L | 537_I | 1.057 | 0.78 |
| 513_Q | 634_R | 1.057 | 0.78 |
| 53_A | 59_I | 1.056 | 0.78 |
| 714_L | 717_N | 1.056 | 0.78 |
| 719_R | 727_L | 1.055 | 0.77 |
| 627_N | 630_S | 1.055 | 0.77 |
| 436_E | 455_R | 1.053 | 0.77 |
| 263_V | 267_T | 1.053 | 0.77 |
| 534_Q | 550_T | 1.053 | 0.77 |
| 208_S | 212_C | 1.053 | 0.77 |
| 585_R | 591_A | 1.050 | 0.77 |
| 465_P | 544_L | 1.050 | 0.77 |
| 503_E | 543_F | 1.047 | 0.77 |
| 488_I | 528_L | 1.045 | 0.77 |
| 657_V | 673_L | 1.043 | 0.76 |
| 513_Q | 553_K | 1.042 | 0.76 |
| 272_L | 332_C | 1.042 | 0.76 |
| 498_L | 506_R | 1.042 | 0.76 |
| 543_F | 624_D | 1.042 | 0.76 |
| 668_A | 706_P | 1.040 | 0.76 |
| 577_A | 596_G | 1.039 | 0.76 |
| 503_E | 703_E | 1.038 | 0.76 |
| 193_Q | 197_C | 1.038 | 0.76 |
| 188_H | 217_G | 1.037 | 0.76 |
| 159_S | 162_L | 1.037 | 0.76 |
| 393_L | 397_L | 1.033 | 0.75 |
| 754_A | 758_A | 1.030 | 0.75 |
| 660_W | 738_Y | 1.029 | 0.75 |
| 42_G | 45_K | 1.029 | 0.75 |
| 195_F | 221_A | 1.029 | 0.75 |
| 215_V | 222_L | 1.027 | 0.75 |
| 195_F | 222_L | 1.027 | 0.75 |
| 336_L | 339_E | 1.026 | 0.75 |
| 473_K | 476_L | 1.024 | 0.74 |
| 281_N | 284_L | 1.021 | 0.74 |
| 518_A | 530_S | 1.021 | 0.74 |
| 146_I | 155_L | 1.017 | 0.74 |
| 332_C | 343_I | 1.016 | 0.74 |
| 144_F | 147_R | 1.015 | 0.74 |
| 593_A | 711_L | 1.015 | 0.74 |
| 554_A | 557_L | 1.014 | 0.73 |
| 210_R | 213_D | 1.014 | 0.73 |
| 749_R | 753_D | 1.012 | 0.73 |
| 633_N | 668_A | 1.012 | 0.73 |
| 495_G | 577_A | 1.012 | 0.73 |
| 22_L | 35_A | 1.011 | 0.73 |
| 549_E | 671_N | 1.009 | 0.73 |
| 594_R | 598_E | 1.008 | 0.73 |
| 19_R | 23_L | 1.008 | 0.73 |
| 573_V | 599_V | 1.007 | 0.73 |
| 535_S | 554_A | 1.006 | 0.73 |
| 612_L | 634_R | 1.004 | 0.72 |
| 280_M | 285_I | 1.003 | 0.72 |
| 588_E | 668_A | 1.003 | 0.72 |
| 276_I | 347_A | 1.002 | 0.72 |
| 265_L | 268_R | 1.000 | 0.72 |
| 512_Q | 674_R | 1.000 | 0.72 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1z6tA | 4 | 0.5236 | 100 | 0.69 | Contact Map |
| 1hz4A | 1 | 0.4541 | 100 | 0.713 | Contact Map |
| 2a5yB | 2 | 0.428 | 100 | 0.726 | Contact Map |
| 2fnaA | 2 | 0.3797 | 100 | 0.764 | Contact Map |
| 2qenA | 1 | 0.3871 | 100 | 0.765 | Contact Map |
| 3sf4A | 3 | 0.4181 | 100 | 0.767 | Contact Map |
| 3ro2A | 1 | 0.3834 | 100 | 0.77 | Contact Map |
| 4gpkA | 12 | 0.428 | 99.9 | 0.788 | Contact Map |
| 4a1sA | 2 | 0.3846 | 99.9 | 0.789 | Contact Map |
| 2xpiA | 2 | 0.5558 | 99.9 | 0.799 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)