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Mad1 -force run -force run

ID: 1415975311 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 718 (702)
Sequences: 248 (197.1)
Seq/Len: 0.353
Nf(neff/√len): 7.4

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 0.353).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
265_E270_R2.3270.90
64_L67_V2.0210.79
512_R515_L1.9580.76
479_M482_S1.9400.75
693_H702_A1.9140.73
380_L383_E1.8970.72
191_Q565_Q1.8290.68
262_L266_S1.7430.63
633_C712_F1.7100.61
665_F675_M1.6990.60
83_V348_L1.6820.59
37_A41_L1.6630.57
625_K643_I1.6510.57
442_V445_V1.6470.56
449_S452_M1.5750.51
36_S40_S1.5750.51
36_S41_L1.5640.51
643_I649_Y1.5620.50
700_I708_T1.5550.50
643_I693_H1.5430.49
123_K128_L1.5400.49
628_E649_Y1.5360.49
281_E421_E1.5220.48
625_K628_E1.5150.47
41_L46_Q1.5140.47
317_W331_P1.5140.47
628_E635_T1.5120.47
52_E56_E1.5040.46
699_S702_A1.4920.45
702_A712_F1.4890.45
504_K509_E1.4850.45
134_C575_L1.4830.45
317_W421_E1.4730.44
712_F715_Q1.4620.43
662_C675_M1.4590.43
35_T38_P1.4510.43
41_L51_L1.4340.41
547_L552_V1.4270.41
421_E675_M1.4230.41
511_E516_E1.4090.40
441_M445_V1.3990.39
628_E704_L1.3990.39
643_I647_N1.3930.39
634_Y654_L1.3870.38
630_R699_S1.3810.38
41_L45_Y1.3800.38
693_H697_Q1.3800.38
694_L699_S1.3750.38
693_H713_S1.3710.37
289_K452_M1.3710.37
306_E311_L1.3700.37
32_D43_M1.3630.37
628_E647_N1.3600.37
262_L442_V1.3560.36
635_T643_I1.3510.36
98_R350_D1.3490.36
604_L615_N1.3450.36
518_E525_Q1.3450.36
38_P42_Q1.3440.35
633_C697_Q1.3410.35
297_Q570_R1.3360.35
699_S712_F1.3350.35
665_F673_S1.3290.35
74_M633_C1.3260.34
628_E652_T1.3090.33
646_E649_Y1.3070.33
255_L709_L1.3060.33
665_F700_I1.3040.33
38_P46_Q1.3010.33
632_A675_M1.2960.32
630_R642_D1.2960.32
694_L711_L1.2940.32
216_E565_Q1.2940.32
649_Y652_T1.2940.32
478_K481_K1.2910.32
369_L488_E1.2910.32
300_L303_L1.2910.32
427_Y430_Q1.2850.32
37_A40_S1.2830.32
616_Q620_E1.2810.32
647_N676_Q1.2800.31
139_D177_E1.2800.31
177_E254_R1.2800.31
701_P708_T1.2780.31
630_R652_T1.2770.31
44_Q51_L1.2770.31
38_P41_L1.2750.31
400_R546_S1.2750.31
40_S48_S1.2740.31
568_C647_N1.2730.31
93_A309_R1.2720.31
630_R665_F1.2700.31
678_L699_S1.2670.31
61_K74_M1.2650.31
438_A441_M1.2640.31
269_L608_V1.2620.30
43_M46_Q1.2590.30
556_R561_H1.2580.30
628_E650_R1.2570.30
635_T647_N1.2570.30
173_V301_V1.2540.30
180_V183_L1.2540.30
248_E541_K1.2530.30
141_A146_R1.2520.30
40_S44_Q1.2520.30
366_R369_L1.2510.30
287_Q611_A1.2490.30
675_M710_E1.2480.30
72_M75_E1.2390.29
435_M438_A1.2330.29
636_L707_L1.2330.29
36_S46_Q1.2310.29
314_L409_D1.2280.28
628_E643_I1.2280.28
155_A159_I1.2270.28
41_L48_S1.2250.28
289_K294_E1.2250.28
187_K190_L1.2240.28
40_S45_Y1.2210.28
477_L575_L1.2190.28
31_L35_T1.2180.28
456_L459_A1.2150.28
122_E126_E1.2150.28
35_T41_L1.2130.28
649_Y685_T1.2120.28
46_Q54_R1.2110.27
486_S489_Q1.2080.27
643_I676_Q1.2040.27
40_S46_Q1.2030.27
355_V607_Q1.2030.27
439_E442_V1.2030.27
666_K676_Q1.2010.27
628_E637_T1.2000.27
301_V398_Q1.2000.27
697_Q702_A1.1990.27
632_A688_E1.1990.27
80_R702_A1.1980.27
568_C643_I1.1940.27
38_P45_Y1.1940.27
530_A535_Y1.1940.27
434_R439_E1.1940.27
82_R633_C1.1930.26
39_G42_Q1.1910.26
608_V611_A1.1910.26
630_R636_L1.1910.26
637_T647_N1.1900.26
82_R646_E1.1830.26
248_E342_Q1.1810.26
417_S421_E1.1810.26
701_P705_S1.1790.26
633_C690_I1.1750.25
42_Q48_S1.1730.25
652_T662_C1.1730.25
585_P589_E1.1700.25
142_S145_L1.1700.25
384_R689_L1.1690.25
690_I708_T1.1680.25
210_E565_Q1.1680.25
86_E415_L1.1650.25
238_D258_E1.1650.25
704_L713_S1.1640.25
145_L149_E1.1630.25
31_L43_M1.1620.25
511_E619_K1.1620.25
138_L668_T1.1620.25
77_S680_T1.1600.25
643_I702_A1.1590.25
459_A694_L1.1580.25
21_I25_V1.1580.25
45_Y48_S1.1570.25
539_R703_F1.1570.25
36_S43_M1.1570.25
42_Q45_Y1.1570.25
299_T304_E1.1570.25
665_F710_E1.1560.24
29_S33_I1.1550.24
680_T694_L1.1550.24
185_S240_A1.1550.24
41_L44_Q1.1540.24
32_D35_T1.1540.24
49_M54_R1.1540.24
41_L47_Q1.1540.24
353_S357_S1.1510.24
262_L400_R1.1500.24
631_K646_E1.1500.24
257_R710_E1.1490.24
635_T649_Y1.1480.24
625_K676_Q1.1480.24
39_G48_S1.1480.24
431_L633_C1.1470.24
29_S35_T1.1440.24
452_M456_L1.1440.24
570_R655_Y1.1430.24
545_M548_N1.1410.24
441_M446_H1.1410.24
30_G35_T1.1410.24
38_P43_M1.1410.24
47_Q51_L1.1410.24
505_V509_E1.1360.23
256_E317_W1.1350.23
650_R699_S1.1350.23
630_R639_Y1.1330.23
43_M47_Q1.1330.23
32_D37_A1.1320.23
278_L281_E1.1300.23
124_M620_E1.1280.23
102_R451_E1.1280.23
44_Q47_Q1.1270.23
225_I228_L1.1270.23
622_F711_L1.1260.23
27_G35_T1.1260.23
353_S705_S1.1260.23
37_A48_S1.1260.23
358_S622_F1.1240.23
94_R282_E1.1230.23
138_L152_L1.1230.23
415_L608_V1.1210.23
691_E696_R1.1210.23
289_K434_R1.1200.23
700_I704_L1.1200.23
607_Q612_E1.1200.23
282_E618_L1.1180.23
632_A662_C1.1180.23
33_I43_M1.1170.23
626_I643_I1.1170.23
649_Y697_Q1.1160.22
313_K317_W1.1160.22
262_L545_M1.1140.22
430_Q620_E1.1130.22
48_S52_E1.1130.22
677_L686_V1.1120.22
692_V696_R1.1120.22
115_E304_E1.1120.22
188_Q191_Q1.1090.22
90_S97_E1.1070.22
287_Q698_D1.1070.22
652_T675_M1.1050.22
418_Y442_V1.1050.22
656_A712_F1.1040.22
23_Q27_G1.1040.22
692_V697_Q1.1030.22
34_S37_A1.1020.22
51_L54_R1.1020.22
214_S637_T1.0990.22
541_K697_Q1.0990.22
47_Q50_Q1.0980.22
650_R702_A1.0980.22
708_T712_F1.0970.22
35_T45_Y1.0960.22
689_L693_H1.0950.21
44_Q49_M1.0940.21
294_E453_E1.0920.21
27_G31_L1.0920.21
275_T636_L1.0910.21
24_R27_G1.0910.21
599_K607_Q1.0910.21
27_G33_I1.0910.21
611_A614_K1.0900.21
180_V214_S1.0890.21
693_H706_S1.0890.21
242_V362_L1.0850.21
68_E190_L1.0840.21
675_M700_I1.0810.21
61_K714_R1.0810.21
649_Y695_R1.0810.21
622_F629_F1.0810.21
317_W629_F1.0810.21
49_M53_E1.0800.21
248_E251_R1.0790.21
637_T692_V1.0780.21
283_L421_E1.0770.21
630_R634_Y1.0760.21
48_S51_L1.0760.21
541_K635_T1.0740.20
47_Q52_E1.0730.20
51_L64_L1.0710.20
274_E412_R1.0700.20
127_Q212_Q1.0700.20
640_Q649_Y1.0700.20
625_K693_H1.0680.20
287_Q612_E1.0680.20
410_G692_V1.0680.20
19_N25_V1.0670.20
647_N675_M1.0660.20
194_L641_I1.0660.20
386_K542_V1.0650.20
667_A675_M1.0650.20
418_Y646_E1.0650.20
35_T39_G1.0640.20
25_V28_G1.0620.20
558_R562_S1.0620.20
253_P651_L1.0610.20
139_D552_V1.0610.20
36_S39_G1.0600.20
656_A699_S1.0600.20
633_C693_H1.0600.20
28_G31_L1.0570.20
647_N665_F1.0570.20
84_E636_L1.0560.20
676_Q702_A1.0560.20
384_R396_R1.0550.20
291_G508_L1.0550.20
37_A42_Q1.0550.20
126_E481_K1.0540.20
143_K234_L1.0530.20
533_G542_V1.0520.19
73_Q273_R1.0520.19
568_C572_R1.0490.19
88_A99_E1.0490.19
155_A158_T1.0490.19
54_R57_Q1.0480.19
620_E634_Y1.0480.19
396_R665_F1.0460.19
628_E675_M1.0440.19
37_A51_L1.0440.19
52_E419_D1.0440.19
27_G30_G1.0420.19
476_E481_K1.0420.19
421_E652_T1.0410.19
214_S344_R1.0410.19
73_Q655_Y1.0410.19
643_I697_Q1.0390.19
28_G35_T1.0390.19
651_L705_S1.0370.19
694_L716_T1.0370.19
138_L142_S1.0370.19
542_V702_A1.0370.19
36_S45_Y1.0360.19
648_Q676_Q1.0360.19
568_C625_K1.0350.19
663_L678_L1.0340.19
295_K397_L1.0340.19
33_I37_A1.0340.19
262_L398_Q1.0340.19
173_V176_Q1.0340.19
435_M439_E1.0330.19
643_I704_L1.0320.19
665_F708_T1.0310.19
92_S523_E1.0310.19
69_R568_C1.0310.19
701_P704_L1.0310.19
28_G33_I1.0300.19
658_H661_D1.0290.19
408_R633_C1.0290.19
255_L397_L1.0290.19
63_H359_A1.0280.18
35_T43_M1.0280.18
25_V29_S1.0270.18
700_I710_E1.0270.18
652_T697_Q1.0260.18
86_E500_T1.0260.18
201_C521_M1.0240.18
77_S557_L1.0240.18
607_Q611_A1.0230.18
122_E351_K1.0230.18
481_K484_S1.0220.18
57_Q64_L1.0200.18
694_L703_F1.0200.18
256_E627_Q1.0200.18
398_Q700_I1.0200.18
278_L342_Q1.0190.18
245_M353_S1.0190.18
370_Q624_T1.0180.18
455_Q590_A1.0180.18
74_M415_L1.0180.18
228_L396_R1.0180.18
29_S34_S1.0160.18
159_I168_E1.0150.18
519_K526_L1.0140.18
692_V713_S1.0130.18
280_Q311_L1.0120.18
19_N23_Q1.0110.18
278_L712_F1.0100.18
85_L89_A1.0090.18
680_T683_S1.0080.18
75_E323_T1.0080.18
673_S686_V1.0080.18
49_M52_E1.0080.18
33_I42_Q1.0070.18
96_Y183_L1.0070.18
283_L286_L1.0070.18
553_A634_Y1.0060.18
31_L34_S1.0050.18
36_S42_Q1.0050.18
219_A222_E1.0050.18
548_N628_E1.0040.17
258_E439_E1.0040.17
693_H696_R1.0030.17
557_L666_K1.0030.17
199_K203_E1.0020.17
536_D539_R1.0020.17
34_S38_P1.0020.17
699_S703_F1.0010.17
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Sep14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4dzoA20.16851000.728Contact Map
1go4E40.139399.80.87Contact Map
1i84S20.200698.80.929Contact Map
1c1gA40.395598.30.941Contact Map
4linA120.35196.30.958Contact Map
4cgkA20.45496.20.958Contact Map
2v71A20.217393.70.964Contact Map
4iloA20.307891.30.967Contact Map
2efrA40.215990.80.967Contact Map
3na7A10.309290.80.967Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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