| DENV2 methyltransferase |
| ID: | 1414490830 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 50% gaps before running GREMLIN. |
| Length: | 305 (137) | ||
| Sequences: | 46104 (29525.3) | ||
| Seq/Len: | 336.526 | ||
| Nf(neff/√len): | 2522.5 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 336.526).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 110_K | 149_C | 3.412 | 1.00 |
| 87_V | 149_C | 3.118 | 1.00 |
| 150_D | 182_S | 3.036 | 1.00 |
| 91_C | 111_G | 2.843 | 1.00 |
| 85_K | 108_E | 2.828 | 1.00 |
| 181_L | 185_T | 2.688 | 1.00 |
| 97_S | 111_G | 2.676 | 1.00 |
| 176_L | 179_N | 2.359 | 1.00 |
| 108_E | 131_N | 2.315 | 1.00 |
| 177_V | 187_F | 2.314 | 1.00 |
| 113_T | 119_H | 2.223 | 1.00 |
| 110_K | 133_V | 2.219 | 1.00 |
| 116_G | 134_R | 2.155 | 1.00 |
| 97_S | 109_V | 2.123 | 1.00 |
| 119_H | 132_L | 2.067 | 1.00 |
| 119_H | 134_R | 2.032 | 1.00 |
| 81_T | 84_G | 1.991 | 1.00 |
| 116_G | 120_E | 1.989 | 1.00 |
| 85_K | 110_K | 1.984 | 1.00 |
| 90_G | 157_G | 1.930 | 1.00 |
| 84_G | 151_T | 1.782 | 1.00 |
| 114_K | 140_D | 1.780 | 1.00 |
| 85_K | 149_C | 1.760 | 1.00 |
| 122_P | 132_L | 1.747 | 1.00 |
| 104_K | 107_R | 1.653 | 1.00 |
| 85_K | 150_D | 1.629 | 1.00 |
| 176_L | 180_W | 1.625 | 1.00 |
| 140_D | 143_F | 1.608 | 1.00 |
| 91_C | 122_P | 1.564 | 1.00 |
| 101_G | 129_G | 1.563 | 1.00 |
| 86_V | 151_T | 1.550 | 1.00 |
| 181_L | 187_F | 1.546 | 1.00 |
| 91_C | 97_S | 1.482 | 1.00 |
| 152_L | 181_L | 1.461 | 1.00 |
| 152_L | 177_V | 1.399 | 0.99 |
| 146_P | 179_N | 1.396 | 0.99 |
| 98_Y | 126_S | 1.393 | 0.99 |
| 110_K | 131_N | 1.386 | 0.99 |
| 86_V | 100_C | 1.362 | 0.99 |
| 89_L | 177_V | 1.352 | 0.99 |
| 88_D | 91_C | 1.350 | 0.99 |
| 121_E | 124_P | 1.345 | 0.99 |
| 85_K | 182_S | 1.327 | 0.99 |
| 90_G | 154_C | 1.277 | 0.99 |
| 98_Y | 125_M | 1.271 | 0.99 |
| 119_H | 123_I | 1.247 | 0.99 |
| 99_Y | 103_L | 1.243 | 0.98 |
| 154_C | 187_F | 1.220 | 0.98 |
| 112_L | 139_V | 1.217 | 0.98 |
| 84_G | 150_D | 1.209 | 0.98 |
| 148_R | 182_S | 1.204 | 0.98 |
| 120_E | 123_I | 1.197 | 0.98 |
| 101_G | 130_W | 1.196 | 0.98 |
| 115_G | 118_G | 1.180 | 0.98 |
| 154_C | 157_G | 1.167 | 0.97 |
| 126_S | 132_L | 1.148 | 0.97 |
| 96_W | 155_D | 1.138 | 0.97 |
| 133_V | 139_V | 1.122 | 0.97 |
| 87_V | 152_L | 1.116 | 0.96 |
| 99_Y | 102_G | 1.111 | 0.96 |
| 80_V | 84_G | 1.109 | 0.96 |
| 148_R | 180_W | 1.100 | 0.96 |
| 117_P | 120_E | 1.098 | 0.96 |
| 85_K | 148_R | 1.085 | 0.96 |
| 152_L | 180_W | 1.049 | 0.94 |
| 88_D | 111_G | 1.042 | 0.94 |
| 111_G | 132_L | 1.034 | 0.94 |
| 89_L | 141_V | 1.027 | 0.94 |
| 93_R | 118_G | 1.012 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3lkzA | 2 | 0.8492 | 100 | 0.125 | Contact Map |
| 2p41A | 1 | 0.8492 | 100 | 0.168 | Contact Map |
| 4k6mA | 2 | 0.9279 | 100 | 0.183 | Contact Map |
| 4ctjA | 2 | 0.8393 | 100 | 0.249 | Contact Map |
| 2px2A | 2 | 0.8295 | 100 | 0.306 | Contact Map |
| 3eldA | 1 | 0.8885 | 100 | 0.315 | Contact Map |
| 2oxtA | 4 | 0.8459 | 100 | 0.341 | Contact Map |
| 2wa2A | 2 | 0.8459 | 100 | 0.421 | Contact Map |
| 3gczA | 1 | 0.8197 | 100 | 0.442 | Contact Map |
| 3evfA | 1 | 0.8295 | 100 | 0.447 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)