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OPENSEQ.org

Mad1

ID: 1411720429 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 718 (669)
Sequences: 4861 (4607.2)
Seq/Len: 7.266
Nf(neff/√len): 178.1

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 7.266).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
561_H564_L2.1611.00
338_V341_L2.0921.00
259_L262_L2.0111.00
512_R515_L1.9431.00
201_C204_A1.9291.00
266_S269_L1.9221.00
451_E455_Q1.9141.00
359_A362_L1.8641.00
340_E344_R1.8571.00
518_E521_M1.8301.00
652_T656_A1.8281.00
601_V605_K1.8201.00
394_A397_L1.7971.00
579_E583_T1.7661.00
373_L376_V1.7511.00
662_C665_F1.7491.00
173_V176_Q1.7421.00
307_N310_L1.7391.00
55_A58_I1.7371.00
366_R369_L1.7031.00
131_N134_C1.6981.00
57_Q61_K1.6721.00
256_E260_K1.6641.00
345_E348_L1.6621.00
283_L286_L1.6571.00
187_K190_L1.6561.00
657_E660_G1.6531.00
82_R85_L1.6421.00
658_H661_D1.6241.00
380_L383_E1.6201.00
367_Q370_Q1.6171.00
575_L578_M1.6161.00
313_K316_S1.6101.00
330_T333_D1.5861.00
652_T655_Y1.5861.00
352_N355_V1.5861.00
396_R400_R1.5821.00
254_R258_E1.5791.00
166_I169_L1.5791.00
207_K210_E1.5701.00
194_L197_Q1.5681.00
449_S452_M1.5491.00
472_M476_E1.5431.00
333_D337_F1.5351.00
69_R72_M1.5341.00
393_L396_R1.5320.99
311_L315_Q1.5310.99
314_L317_W1.5270.99
651_L656_A1.5260.99
442_V445_V1.5250.99
233_S236_E1.5180.99
601_V604_L1.5170.99
567_E571_L1.5150.99
526_L529_R1.5120.99
457_S461_E1.5040.99
474_E478_K1.5000.99
126_E130_R1.4940.99
91_T95_N1.4930.99
347_A351_K1.4930.99
70_E74_M1.4860.99
505_V508_L1.4790.99
408_R412_R1.4780.99
119_G123_K1.4760.99
568_C571_L1.4740.99
62_S65_I1.4610.99
456_L459_A1.4590.99
283_L287_Q1.4550.99
152_L155_A1.4520.99
657_E661_D1.4520.99
554_R557_L1.4490.99
117_E120_A1.4400.99
434_R437_E1.4400.99
491_F494_S1.4350.99
375_Q379_Q1.4320.99
208_I211_L1.4130.99
149_E153_A1.4120.99
110_I113_L1.4110.99
401_V404_L1.4110.99
525_Q528_R1.4080.99
223_Q227_D1.4060.99
189_E193_Q1.4030.99
63_H66_Q1.3990.99
410_G414_I1.3980.99
479_M483_Q1.3770.99
360_R363_E1.3720.99
374_R377_S1.3710.99
299_T302_G1.3710.99
619_K623_Q1.3700.99
444_K448_H1.3700.99
437_E441_M1.3680.99
275_T278_L1.3640.99
361_G365_A1.3620.99
176_Q179_R1.3620.99
409_D412_R1.3600.99
235_Q239_A1.3600.99
477_L480_L1.3590.99
172_S176_Q1.3570.98
651_L655_Y1.3570.98
507_E511_E1.3560.98
253_P256_E1.3550.98
657_E665_F1.3530.98
657_E662_C1.3510.98
570_R574_L1.3490.98
40_S44_Q1.3480.98
349_K353_S1.3480.98
520_R523_E1.3470.98
436_R439_E1.3460.98
402_L405_T1.3440.98
98_R102_R1.3400.98
199_K203_E1.3340.98
89_A92_S1.3340.98
133_Q137_N1.3340.98
342_Q346_L1.3310.98
331_P334_L1.3300.98
399_K403_L1.3280.98
368_Q372_E1.3270.98
222_E226_K1.3270.98
661_D667_A1.3270.98
154_Q158_T1.3260.98
97_E101_D1.3230.98
395_R398_Q1.3230.98
139_D142_S1.3200.98
443_Q446_H1.3200.98
357_S361_G1.3200.98
135_Q139_D1.3190.98
225_I229_E1.3180.98
93_A97_E1.3170.98
553_A556_R1.3110.98
105_E109_R1.3060.98
481_K485_S1.3050.98
138_L141_A1.3040.98
188_Q191_Q1.3010.98
373_L377_S1.3000.98
231_K235_Q1.3000.98
608_V611_A1.2990.98
394_A398_Q1.2980.98
165_R170_Q1.2970.98
186_E189_E1.2950.98
661_D665_F1.2940.98
250_V254_R1.2940.98
263_R267_A1.2930.98
435_M438_A1.2920.98
124_M127_Q1.2860.98
182_R186_E1.2840.98
610_S614_K1.2840.98
418_Y421_E1.2790.98
226_K229_E1.2770.98
26_E30_G1.2760.98
69_R73_Q1.2740.97
50_Q53_E1.2720.97
251_R256_E1.2720.97
167_S170_Q1.2710.97
53_E57_Q1.2690.97
22_S30_G1.2690.97
42_Q45_Y1.2680.97
493_F497_E1.2620.97
262_L265_E1.2580.97
300_L303_L1.2570.97
180_V183_L1.2550.97
512_R516_E1.2540.97
443_Q447_S1.2510.97
415_L419_D1.2500.97
115_E118_A1.2490.97
227_D231_K1.2480.97
315_Q318_E1.2460.97
86_E90_S1.2460.97
298_E302_G1.2430.97
382_E386_K1.2410.97
112_Q116_R1.2410.97
165_R168_E1.2390.97
145_L148_K1.2390.97
433_R437_E1.2390.97
200_K204_A1.2340.97
660_G665_F1.2330.97
511_E514_R1.2320.97
498_A501_L1.2320.97
478_K481_K1.2310.97
611_A615_N1.2300.97
577_A581_G1.2300.97
662_C667_A1.2280.97
132_R135_Q1.2280.97
568_C572_R1.2250.97
261_Q265_E1.2240.97
48_S51_L1.2210.97
306_E309_R1.2210.97
661_D666_K1.2180.96
122_E126_E1.2150.96
667_A671_S1.2120.96
519_K522_L1.2110.96
446_H450_A1.2100.96
147_E151_S1.2090.96
667_A672_G1.2090.96
243_K247_S1.2070.96
117_E121_E1.2070.96
158_T161_A1.2070.96
252_L256_E1.2070.96
486_S490_S1.2050.96
379_Q382_E1.2040.96
195_D198_H1.2040.96
186_E191_Q1.2040.96
442_V446_H1.2000.96
301_V304_E1.1990.96
123_K126_E1.1980.96
514_R518_E1.1960.96
131_N135_Q1.1940.96
72_M75_E1.1930.96
234_L238_D1.1920.96
655_Y658_H1.1910.96
109_R113_L1.1900.96
353_S356_T1.1890.96
232_L235_Q1.1860.96
187_K191_Q1.1860.96
504_K509_E1.1850.96
573_G577_A1.1850.96
258_E261_Q1.1840.96
391_E395_R1.1820.96
412_R416_G1.1820.96
284_E287_Q1.1800.96
66_Q70_E1.1800.96
282_E285_G1.1780.95
196_L200_K1.1770.95
458_Q462_E1.1750.95
79_K82_R1.1750.95
558_R562_S1.1740.95
205_N209_Q1.1730.95
41_L44_Q1.1730.95
574_L579_E1.1720.95
390_H393_L1.1710.95
141_A144_R1.1700.95
177_E181_K1.1700.95
294_E297_Q1.1690.95
456_L460_L1.1660.95
332_E335_S1.1650.95
356_T360_R1.1630.95
18_N27_G1.1630.95
160_N163_K1.1620.95
662_C666_K1.1620.95
556_R560_D1.1610.95
416_G419_D1.1590.95
92_S95_N1.1550.95
660_G667_A1.1550.95
502_R506_E1.1520.95
614_K617_R1.1500.95
54_R59_R1.1490.95
506_E509_E1.1480.95
297_Q301_V1.1480.95
209_Q212_Q1.1470.95
304_E308_E1.1470.95
200_K203_E1.1460.95
495_R499_D1.1440.94
416_G420_S1.1430.94
401_V405_T1.1430.94
407_E410_G1.1420.94
251_R254_R1.1410.94
103_N106_L1.1390.94
17_L20_F1.1350.94
302_G306_E1.1340.94
575_L579_E1.1340.94
184_E188_Q1.1340.94
584_V588_L1.1320.94
662_C668_T1.1310.94
282_E287_Q1.1290.94
656_A660_G1.1290.94
434_R439_E1.1280.94
307_N311_L1.1260.94
644_T649_Y1.1250.94
441_M445_V1.1250.94
245_M249_L1.1240.94
175_D179_R1.1240.94
504_K507_E1.1230.94
203_E207_K1.1230.94
240_A244_N1.1220.94
605_K609_E1.1220.94
439_E443_Q1.1190.94
358_S361_G1.1190.94
660_G663_L1.1170.94
140_A144_R1.1170.94
582_G586_A1.1170.94
658_H662_C1.1160.93
262_L266_S1.1140.93
415_L418_Y1.1140.93
518_E523_E1.1120.93
404_L408_R1.1120.93
248_E251_R1.1100.93
430_Q434_R1.1090.93
606_K610_S1.1080.93
224_Q229_E1.1080.93
572_R575_L1.1080.93
308_E312_A1.1070.93
13_T16_S1.1060.93
54_R57_Q1.1060.93
629_F641_I1.1060.93
235_Q238_D1.1010.93
202_Q205_N1.0980.93
255_L259_L1.0950.93
179_R184_E1.0940.93
159_I162_L1.0930.93
408_R411_M1.0920.92
626_I630_R1.0900.92
103_N107_L1.0890.92
107_L111_R1.0890.92
496_E500_T1.0890.92
70_E73_Q1.0880.92
96_Y99_E1.0870.92
36_S40_S1.0860.92
90_S93_A1.0860.92
116_R120_A1.0830.92
521_M525_Q1.0810.92
651_L654_L1.0810.92
371_E375_Q1.0810.92
163_K167_S1.0800.92
146_R149_E1.0790.92
295_K299_T1.0740.92
224_Q227_D1.0710.92
245_M248_E1.0710.92
498_A502_R1.0700.91
18_N22_S1.0680.91
38_P42_Q1.0680.91
657_E666_K1.0670.91
658_H665_F1.0660.91
649_Y652_T1.0660.91
282_E286_L1.0650.91
159_I163_K1.0650.91
280_Q284_E1.0650.91
666_K672_G1.0640.91
497_E501_L1.0630.91
134_C137_N1.0630.91
18_N26_E1.0620.91
303_L306_E1.0600.91
656_A661_D1.0600.91
174_M177_E1.0600.91
99_E103_N1.0580.91
660_G666_K1.0550.91
457_S460_L1.0540.91
650_R654_L1.0540.91
39_G42_Q1.0530.91
505_V509_E1.0530.91
649_Y654_L1.0530.91
170_Q174_M1.0520.91
232_L236_E1.0520.91
435_M439_E1.0510.90
485_S488_E1.0510.90
583_T587_D1.0480.90
248_E252_L1.0460.90
198_H202_Q1.0450.90
663_L666_K1.0450.90
440_D444_K1.0440.90
549_P553_A1.0420.90
413_A417_S1.0420.90
337_F340_E1.0420.90
124_M128_L1.0410.90
470_A474_E1.0410.90
632_A636_L1.0410.90
390_H394_A1.0400.90
128_L132_R1.0390.90
655_Y660_G1.0380.90
500_T504_K1.0380.90
332_E336_R1.0370.90
44_Q47_Q1.0350.90
405_T409_D1.0320.89
452_M455_Q1.0300.89
657_E667_A1.0300.89
150_D153_A1.0290.89
47_Q51_L1.0290.89
252_L255_L1.0270.89
350_D354_A1.0260.89
573_G576_R1.0260.89
386_K389_T1.0260.89
650_R656_A1.0240.89
417_S421_E1.0240.89
628_E631_K1.0240.89
90_S94_R1.0230.89
71_K75_E1.0200.89
106_L109_R1.0200.89
379_Q383_E1.0180.89
462_E465_G1.0140.88
164_G168_E1.0130.88
630_R634_Y1.0130.88
483_Q486_S1.0120.88
363_E367_Q1.0110.88
515_L519_K1.0110.88
598_S602_A1.0100.88
663_L667_A1.0100.88
582_G585_P1.0090.88
660_G668_T1.0080.88
179_R183_L1.0070.88
88_A93_A1.0050.88
484_S488_E1.0050.88
404_L407_E1.0050.88
161_A165_R1.0050.88
320_L324_M1.0040.88
354_A358_S1.0040.88
434_R438_A1.0030.88
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Sep14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4dzoA20.168599.90.711Contact Map
1i84S20.160299.30.83Contact Map
1c1gA40.395599.30.833Contact Map
3ktaB20.143597.80.897Contact Map
1hciA20.586497.30.906Contact Map
1u4qA20.42997.30.907Contact Map
3na7A10.318997.20.908Contact Map
3kbtA20.394297.20.908Contact Map
3edvA20.433197.10.91Contact Map
3r6nA20.536296.70.914Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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