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OPENSEQ.org

A0LXY7_GRAFK -force run

ID: 1407783562 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 902 (857)
Sequences: 358 (333.3)
Seq/Len: 0.418
Nf(neff/√len): 11.4

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 0.418).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
46_V71_I3.1040.99
49_K68_D2.7200.97
115_A160_S2.4890.95
38_M46_V2.4420.94
6_K10_F2.2130.89
674_A715_L1.9580.80
573_N593_N1.8390.74
561_A564_V1.7260.67
735_D800_N1.6890.64
114_K159_N1.6550.62
93_S96_E1.6490.62
514_S518_F1.6440.61
59_F62_A1.6300.60
121_V127_A1.6190.59
479_G510_S1.5940.58
86_E114_K1.5750.56
836_R839_K1.5570.55
122_D126_N1.5430.54
844_K849_A1.5330.53
759_K762_G1.5290.53
844_K847_S1.5100.52
847_S852_S1.5060.51
330_P605_V1.5050.51
54_S59_F1.4950.50
238_A553_L1.4920.50
820_L823_K1.4790.49
624_D655_L1.4790.49
850_V854_K1.4770.49
274_F738_V1.4570.48
605_V657_G1.4480.47
614_F657_G1.4420.47
695_D699_N1.4290.46
157_I160_S1.4190.45
657_G713_T1.4120.44
835_K840_K1.4090.44
303_G437_G1.4060.44
835_K838_P1.4050.44
681_A688_A1.4030.44
63_T121_V1.3960.43
317_I340_M1.3930.43
66_I771_K1.3880.43
752_L799_L1.3850.42
844_K848_T1.3840.42
836_R849_A1.3680.41
822_G830_D1.3670.41
852_S855_T1.3660.41
462_L724_I1.3650.41
846_D850_V1.3640.41
64_L385_L1.3620.41
388_K415_D1.3520.40
25_I34_V1.3520.40
842_F848_T1.3500.40
157_I162_V1.3500.40
842_F847_S1.3490.40
42_L119_I1.3420.39
681_A800_N1.3420.39
330_P657_G1.3400.39
822_G832_L1.3400.39
851_N858_T1.3390.39
18_L157_I1.3390.39
437_G464_F1.3380.39
735_D752_L1.3370.39
839_K854_K1.3350.39
752_L788_I1.3290.38
278_I752_L1.3240.38
437_G487_G1.3230.38
847_S855_T1.3230.38
17_I26_F1.3170.38
841_P846_D1.3170.38
233_F466_Q1.3150.38
34_V42_L1.3100.37
843_N849_A1.3070.37
837_D847_S1.3020.37
696_D699_N1.3020.37
596_N606_S1.2940.36
752_L801_M1.2910.36
39_N131_I1.2910.36
848_T854_K1.2860.36
839_K847_S1.2840.35
317_I748_M1.2810.35
227_E797_I1.2810.35
409_N416_I1.2800.35
827_A836_R1.2760.35
836_R843_N1.2740.35
853_Q857_S1.2700.34
155_Y274_F1.2660.34
278_I801_M1.2660.34
843_N847_S1.2640.34
848_T852_S1.2610.34
28_S31_K1.2610.34
845_N849_A1.2590.34
748_M788_I1.2570.34
839_K844_K1.2570.34
854_K857_S1.2570.34
278_I727_M1.2560.34
849_A852_S1.2540.33
847_S850_V1.2540.33
11_V18_L1.2510.33
735_D780_T1.2430.33
69_L85_G1.2420.33
731_I786_I1.2410.33
844_K855_T1.2390.32
479_G517_D1.2350.32
460_A474_L1.2340.32
828_I836_R1.2320.32
833_Q836_R1.2310.32
175_I178_L1.2270.32
674_A678_L1.2270.32
502_L510_S1.2270.32
836_R846_D1.2260.32
261_D502_L1.2240.31
830_D833_Q1.2240.31
278_I780_T1.2210.31
674_A681_A1.2200.31
818_K823_K1.2190.31
481_T510_S1.2190.31
344_N437_G1.2190.31
752_L781_T1.2170.31
50_D67_E1.2170.31
809_T813_V1.2140.31
844_K852_S1.2140.31
35_K48_F1.2130.31
837_D841_P1.2110.31
322_A582_D1.2090.31
695_D702_N1.2090.31
706_I797_I1.2080.30
278_I800_N1.2080.30
434_K585_A1.2060.30
640_P720_G1.2050.30
640_P727_M1.2040.30
83_A313_M1.2030.30
113_N158_N1.2010.30
86_E473_N1.2010.30
618_L653_L1.1990.30
847_S854_K1.1990.30
338_L768_T1.1960.30
834_G858_T1.1950.30
17_I145_S1.1940.30
733_G757_P1.1940.30
258_T783_E1.1900.29
262_V772_L1.1900.29
802_Q806_N1.1900.29
841_P848_T1.1890.29
815_Q819_N1.1890.29
728_N739_A1.1880.29
835_K841_P1.1880.29
125_G166_D1.1870.29
838_P844_K1.1860.29
833_Q838_P1.1860.29
590_I720_G1.1860.29
845_N848_T1.1850.29
115_A326_Y1.1840.29
425_I797_I1.1840.29
90_L446_Y1.1810.29
742_H750_Y1.1800.29
313_M795_P1.1790.29
655_L670_L1.1760.29
677_E710_K1.1760.29
815_Q834_G1.1760.29
110_L754_L1.1740.28
81_T430_Y1.1730.28
849_A857_S1.1710.28
845_N850_V1.1680.28
840_K849_A1.1670.28
819_N835_K1.1660.28
811_K814_A1.1660.28
31_K50_D1.1660.28
850_V853_Q1.1660.28
317_I324_F1.1660.28
818_K822_G1.1650.28
846_D854_K1.1640.28
843_N854_K1.1630.28
855_T859_G1.1630.28
850_V857_S1.1620.28
210_Y499_D1.1620.28
843_N850_V1.1610.28
846_D849_A1.1610.28
625_I632_L1.1590.28
845_N857_S1.1580.27
831_I834_G1.1580.27
853_Q858_T1.1560.27
155_Y180_H1.1560.27
843_N856_D1.1540.27
124_L680_T1.1530.27
166_D249_G1.1530.27
844_K850_V1.1530.27
441_L460_A1.1530.27
837_D849_A1.1520.27
717_F724_I1.1520.27
162_V382_L1.1510.27
250_Y683_I1.1510.27
839_K842_F1.1510.27
755_D758_A1.1500.27
812_V821_E1.1480.27
110_L433_V1.1480.27
249_G284_K1.1470.27
660_N664_T1.1460.27
849_A854_K1.1460.27
847_S858_T1.1450.27
119_I462_L1.1440.27
91_E148_F1.1410.26
261_D326_Y1.1410.26
97_L792_F1.1400.26
781_T786_I1.1380.26
377_I743_G1.1380.26
833_Q842_F1.1350.26
291_T504_G1.1340.26
71_I246_T1.1340.26
780_T801_M1.1330.26
835_K848_T1.1330.26
114_K170_K1.1330.26
839_K850_V1.1330.26
812_V819_N1.1300.26
12_I571_L1.1290.26
836_R851_N1.1290.26
828_I834_G1.1280.26
396_I489_L1.1280.26
841_P851_N1.1280.26
831_I846_D1.1280.26
818_K833_Q1.1280.26
657_G662_D1.1280.26
849_A855_T1.1270.26
834_G840_K1.1250.26
6_K18_L1.1240.26
756_V759_K1.1230.25
831_I845_N1.1230.25
838_P846_D1.1230.25
829_N842_F1.1220.25
834_G845_N1.1210.25
826_D835_K1.1210.25
839_K845_N1.1210.25
840_K847_S1.1210.25
769_L773_S1.1180.25
844_K856_D1.1180.25
313_M724_I1.1180.25
831_I836_R1.1180.25
752_L780_T1.1150.25
851_N854_K1.1140.25
838_P848_T1.1130.25
460_A657_G1.1130.25
851_N859_G1.1120.25
836_R850_V1.1080.25
815_Q826_D1.1070.25
37_T40_D1.1060.25
333_V678_L1.1060.25
49_K69_L1.1050.24
838_P850_V1.1050.24
846_D852_S1.1030.24
848_T857_S1.1030.24
550_P628_S1.1020.24
822_G829_N1.1020.24
821_E829_N1.1010.24
839_K857_S1.1010.24
152_L674_A1.1000.24
849_A853_Q1.0990.24
278_I735_D1.0990.24
839_K843_N1.0990.24
825_V831_I1.0990.24
709_S746_M1.0970.24
98_F119_I1.0960.24
855_T858_T1.0950.24
721_M743_G1.0940.24
854_K859_G1.0940.24
815_Q822_G1.0930.24
812_V824_A1.0930.24
810_Q821_E1.0930.24
850_V855_T1.0930.24
572_K699_N1.0910.24
828_I832_L1.0900.24
834_G842_F1.0890.24
828_I847_S1.0890.24
4_A7_I1.0880.24
849_A856_D1.0860.23
846_D855_T1.0860.23
847_S857_S1.0860.23
834_G843_N1.0860.23
809_T814_A1.0860.23
822_G841_P1.0850.23
161_R174_D1.0850.23
11_V22_A1.0840.23
164_Y275_L1.0840.23
766_G769_L1.0830.23
261_D302_F1.0830.23
36_K135_D1.0830.23
278_I781_T1.0830.23
225_D232_T1.0820.23
854_K858_T1.0820.23
46_V160_S1.0810.23
4_A9_G1.0810.23
321_N603_G1.0800.23
835_K859_G1.0790.23
605_V614_F1.0790.23
856_D859_G1.0790.23
568_N691_L1.0790.23
830_D837_D1.0780.23
326_Y504_G1.0780.23
848_T851_N1.0780.23
829_N850_V1.0760.23
66_I590_I1.0760.23
605_V674_A1.0760.23
841_P854_K1.0760.23
843_N846_D1.0760.23
837_D845_N1.0760.23
519_M683_I1.0750.23
813_V826_D1.0750.23
287_E735_D1.0740.23
207_G211_L1.0740.23
823_K830_D1.0730.23
275_L745_D1.0720.23
824_A832_L1.0720.23
107_I110_L1.0710.23
15_I544_K1.0700.23
835_K843_N1.0690.23
148_F164_Y1.0680.23
840_K843_N1.0680.23
833_Q839_K1.0680.23
601_L649_L1.0680.23
803_Q807_N1.0680.23
115_A157_I1.0680.23
285_N289_V1.0670.22
401_P750_Y1.0670.22
842_F845_N1.0670.22
842_F850_V1.0660.22
439_A483_I1.0660.22
387_L749_N1.0650.22
655_L701_I1.0640.22
162_V582_D1.0630.22
734_I781_T1.0630.22
839_K846_D1.0630.22
7_I12_I1.0630.22
415_D446_Y1.0630.22
460_A473_N1.0630.22
821_E824_A1.0630.22
834_G841_P1.0620.22
849_A858_T1.0610.22
843_N855_T1.0610.22
824_A834_G1.0610.22
818_K832_L1.0610.22
34_V37_T1.0610.22
48_F494_G1.0610.22
94_I683_I1.0580.22
61_E511_N1.0580.22
278_I681_A1.0580.22
319_S756_V1.0580.22
240_V247_F1.0570.22
578_L741_S1.0560.22
840_K856_D1.0560.22
814_A822_G1.0560.22
852_S858_T1.0540.22
15_I575_K1.0540.22
802_Q805_V1.0530.22
18_L25_I1.0520.22
755_D759_K1.0510.22
841_P852_S1.0500.22
635_M742_H1.0490.22
313_M742_H1.0480.22
459_N473_N1.0480.22
44_A96_E1.0470.21
487_G502_L1.0470.21
831_I856_D1.0470.21
814_A830_D1.0460.21
439_A717_F1.0460.21
835_K850_V1.0460.21
767_N773_S1.0460.21
836_R844_K1.0460.21
842_F846_D1.0450.21
835_K858_T1.0440.21
841_P857_S1.0430.21
331_K786_I1.0430.21
812_V828_I1.0430.21
510_S515_V1.0430.21
164_Y728_N1.0430.21
88_V341_E1.0430.21
851_N856_D1.0420.21
200_L205_Y1.0410.21
731_I799_L1.0400.21
843_N848_T1.0400.21
823_K837_D1.0400.21
832_L836_R1.0390.21
837_D843_N1.0390.21
832_L843_N1.0380.21
6_K691_L1.0380.21
392_N653_L1.0380.21
491_N629_F1.0370.21
837_D842_F1.0360.21
743_G746_M1.0360.21
829_N840_K1.0340.21
46_V72_I1.0330.21
827_A830_D1.0330.21
844_K851_N1.0330.21
818_K835_K1.0330.21
845_N856_D1.0330.21
839_K855_T1.0330.21
167_K749_N1.0330.21
37_T43_N1.0320.21
781_T801_M1.0320.21
477_T515_V1.0300.21
605_V662_D1.0300.21
841_P844_K1.0290.21
835_K849_A1.0290.21
832_L841_P1.0290.21
841_P858_T1.0280.21
50_D222_I1.0270.20
814_A823_K1.0260.20
354_I477_T1.0260.20
842_F852_S1.0250.20
823_K836_R1.0250.20
261_D703_F1.0250.20
31_K38_M1.0240.20
807_N811_K1.0240.20
848_T856_D1.0240.20
512_T518_F1.0230.20
852_S857_S1.0230.20
815_Q838_P1.0230.20
809_T817_K1.0230.20
820_L830_D1.0220.20
823_K838_P1.0210.20
852_S859_G1.0200.20
147_P591_S1.0190.20
822_G833_Q1.0180.20
806_N810_Q1.0180.20
841_P855_T1.0180.20
735_D801_M1.0180.20
624_D648_K1.0170.20
815_Q818_K1.0170.20
104_P708_L1.0160.20
5_L8_L1.0160.20
786_I799_L1.0150.20
66_I152_L1.0150.20
163_K390_T1.0150.20
485_A788_I1.0150.20
838_P847_S1.0130.20
843_N853_Q1.0130.20
287_E801_M1.0130.20
550_P607_T1.0130.20
838_P856_D1.0120.20
826_D829_N1.0100.20
845_N853_Q1.0100.20
289_V752_L1.0090.20
496_L660_N1.0080.20
361_F364_D1.0080.20
657_G792_F1.0080.20
233_F455_V1.0080.20
841_P845_N1.0080.20
829_N841_P1.0080.20
840_K844_K1.0070.20
440_S805_V1.0070.20
841_P850_V1.0070.20
752_L762_G1.0070.20
167_K822_G1.0070.20
9_G16_I1.0070.20
107_I678_L1.0060.19
83_A713_T1.0050.19
851_N855_T1.0050.19
818_K831_I1.0050.19
74_N571_L1.0050.19
837_D846_D1.0050.19
833_Q843_N1.0050.19
815_Q844_K1.0050.19
832_L846_D1.0050.19
5_L9_G1.0040.19
643_S828_I1.0040.19
829_N835_K1.0030.19
838_P843_N1.0030.19
835_K847_S1.0030.19
51_I490_Q1.0030.19
478_T520_V1.0020.19
813_V823_K1.0020.19
812_V823_K1.0010.19
394_A438_T1.0000.19
577_L596_N1.0000.19
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (17Jul14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1s5lU20.10256.10.978Contact Map
2j5lA1020.50.983Contact Map
1spfA10.028817.60.983Contact Map
1ej6B20.06117.10.983Contact Map
4efaE10.11211.50.984Contact Map
3v6iA20.106411.50.984Contact Map
2lk9A10.026610.20.985Contact Map
4jkvA20.1128.10.986Contact Map
1b6aA107.30.986Contact Map
1m56D20.02667.30.986Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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