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OPENSEQ.org

B4F292_PROMH

ID: 1407723711 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 1272 (1270)
Sequences: 1493 (1227.4)
Seq/Len: 1.176
Nf(neff/√len): 34.4

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.176).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
610_V626_I4.2071.00
49_N62_G3.6281.00
984_F993_L2.7151.00
941_L966_L2.6781.00
1220_S1228_Q2.6511.00
840_V853_G2.5211.00
29_Q32_L2.4080.99
903_L917_G2.3700.99
459_N487_T2.3600.99
1098_R1116_E2.3120.99
555_A577_V2.2970.99
1116_E1133_K2.2630.99
1145_I1156_A2.2500.99
999_I1002_A2.2080.98
1227_L1246_Y2.1520.98
962_N981_D2.1410.98
853_G885_L2.1390.98
1062_L1081_V2.1240.98
1061_R1085_R2.1180.98
963_G980_P2.0830.98
786_V815_A2.0700.97
1202_S1221_G2.0450.97
458_W488_G2.0350.97
887_G892_P2.0120.97
719_C728_C1.9760.96
1077_L1108_V1.9730.96
769_G788_L1.9670.96
1093_Q1119_R1.9600.96
28_T31_G1.9300.96
458_W486_T1.9020.95
28_T32_L1.8900.95
838_G855_V1.8870.95
1064_G1106_G1.8840.95
1110_Q1139_D1.8770.95
994_D1040_Q1.8180.94
75_G100_D1.7870.93
979_N1000_P1.7820.93
888_N892_P1.7810.93
996_R1044_A1.7750.92
1231_Y1265_D1.7600.92
561_G565_L1.7590.92
727_L745_L1.7570.92
982_L995_G1.7570.92
729_V743_I1.7540.92
1089_K1094_D1.7510.92
561_G567_P1.7460.92
1249_M1252_L1.7300.91
1131_G1146_F1.7210.91
1068_V1077_L1.7140.91
1019_D1119_R1.7130.91
992_K1040_Q1.6880.90
1095_L1131_G1.6810.89
1110_Q1137_L1.6680.89
647_G652_H1.6620.89
612_G624_L1.6600.89
257_G260_P1.6540.88
1042_N1067_K1.6410.88
439_A442_G1.6200.87
745_L748_F1.6190.87
784_A817_I1.6150.87
749_D752_M1.6030.86
40_A44_P1.5980.86
743_I771_A1.5900.86
790_G793_V1.5850.85
994_D1042_N1.5800.85
980_P997_I1.5770.85
900_I903_L1.5660.84
698_L701_A1.5630.84
856_D882_Q1.5550.84
1222_K1225_D1.5480.84
25_I28_T1.5370.83
1179_M1183_L1.5370.83
1234_G1237_D1.5330.83
1002_A1051_V1.5300.83
462_L484_I1.5260.82
26_L29_Q1.5260.82
1088_F1141_P1.5250.82
1095_L1119_R1.5230.82
488_G497_L1.5210.82
1099_K1114_N1.5180.82
401_Y425_G1.5030.81
826_W838_G1.5000.81
482_G488_G1.4970.81
101_V141_T1.4960.81
980_P1232_G1.4920.80
750_L765_G1.4920.80
1268_Y1272_F1.4840.80
469_T478_A1.4820.80
936_T966_L1.4810.80
1106_G1265_D1.4770.79
430_I450_A1.4760.79
1095_L1245_R1.4730.79
1057_G1092_G1.4590.78
26_L32_L1.4550.78
1135_T1144_E1.4510.78
1228_Q1247_R1.4510.78
1119_R1259_G1.4460.77
958_V983_V1.4460.77
1097_V1115_I1.4400.77
1222_K1228_Q1.4360.77
665_G673_F1.4300.76
539_N559_A1.4270.76
442_G467_I1.4230.76
1121_P1159_Y1.4230.76
1155_E1159_Y1.4230.76
1101_Q1114_N1.4180.75
423_A430_I1.4120.75
1232_G1243_T1.4110.75
466_G479_K1.4080.75
998_D1046_N1.4070.75
26_L31_G1.4060.75
1257_V1263_A1.4050.74
323_K329_L1.4010.74
876_Q904_K1.3980.74
119_S122_L1.3950.74
1019_D1131_G1.3940.74
27_G32_L1.3920.73
1252_L1266_L1.3870.73
594_A630_Q1.3860.73
857_I881_A1.3820.73
103_V143_V1.3820.73
32_L35_A1.3810.73
1159_Y1165_G1.3800.73
1106_G1117_A1.3730.72
138_L141_T1.3710.72
470_A478_A1.3670.72
372_S390_T1.3660.71
444_A464_I1.3640.71
687_T720_W1.3610.71
745_L769_G1.3560.71
1046_N1065_A1.3530.70
652_H680_W1.3480.70
914_I934_I1.3480.70
1119_R1131_G1.3440.70
98_T101_V1.3430.70
118_P121_P1.3420.70
1095_L1259_G1.3410.69
765_G792_G1.3380.69
1206_L1221_G1.3380.69
1093_Q1106_G1.3320.69
974_V1156_A1.3320.69
851_L887_G1.3310.69
904_K913_D1.3310.69
423_A448_G1.3290.68
775_W782_P1.3260.68
1100_G1117_A1.3240.68
1249_M1253_Y1.3230.68
1156_A1233_M1.3220.68
40_A43_V1.3220.68
1245_R1255_Q1.3190.68
927_T946_N1.3180.68
775_W779_G1.3180.68
854_T884_R1.3150.67
960_A983_V1.3110.67
967_R970_L1.3110.67
594_A597_I1.3100.67
1233_M1236_F1.3080.67
415_P442_G1.3070.67
1003_R1050_D1.3060.67
1134_V1137_L1.3030.66
1060_A1083_I1.3020.66
696_W722_N1.3010.66
911_P1110_Q1.3000.66
1192_V1246_Y1.3000.66
1141_P1200_G1.2980.66
1181_I1184_G1.2970.66
1093_Q1265_D1.2940.65
696_W725_A1.2930.65
1230_K1243_T1.2930.65
1151_K1154_Q1.2920.65
1155_E1165_G1.2910.65
1062_L1119_R1.2900.65
1151_K1159_Y1.2900.65
1227_L1244_L1.2870.65
196_V201_A1.2860.65
936_T1119_R1.2850.65
432_L448_G1.2840.65
25_I29_Q1.2830.65
1158_S1272_F1.2830.65
1113_L1134_V1.2820.64
1047_I1051_V1.2810.64
478_A508_L1.2790.64
1057_G1095_L1.2760.64
706_L713_V1.2730.64
99_R139_A1.2730.64
76_E99_R1.2720.64
713_V1266_L1.2690.63
899_G920_D1.2690.63
631_L638_I1.2690.63
941_L1221_G1.2670.63
1260_M1263_A1.2660.63
1132_V1223_I1.2620.63
591_D611_K1.2610.63
902_Q916_K1.2600.62
1098_R1118_I1.2570.62
980_P1146_F1.2550.62
1047_I1064_G1.2540.62
996_R1046_N1.2530.62
1090_A1095_L1.2530.62
439_A497_L1.2510.62
1081_V1097_V1.2500.62
980_P1236_F1.2500.62
739_G775_W1.2480.61
1088_F1119_R1.2480.61
1164_E1267_L1.2470.61
1230_K1265_D1.2460.61
797_Q1095_L1.2440.61
958_V985_E1.2430.61
464_I482_G1.2390.61
997_I1045_I1.2370.60
1246_Y1267_L1.2370.60
228_K231_L1.2360.60
809_D1265_D1.2360.60
1257_V1262_Q1.2350.60
936_T1111_P1.2350.60
545_G581_G1.2340.60
682_G716_G1.2340.60
1064_G1111_P1.2320.60
416_P441_Q1.2310.60
1088_F1130_A1.2280.59
10_T14_I1.2260.59
210_T213_E1.2250.59
973_M1062_L1.2250.59
102_I142_R1.2230.59
1176_M1187_Q1.2210.59
448_G460_A1.2210.59
815_A1102_I1.2200.59
1104_F1163_G1.2200.59
846_E1180_L1.2180.59
1070_Q1075_L1.2180.59
1221_G1231_Y1.2150.58
1231_Y1236_F1.2150.58
446_I460_A1.2150.58
980_P1159_Y1.2140.58
1057_G1259_G1.2140.58
1091_Y1265_D1.2120.58
1196_G1200_G1.2100.58
1231_Y1242_L1.2100.58
790_G794_G1.2090.58
1064_G1090_A1.2080.58
881_A973_M1.2070.58
865_I875_A1.2050.57
1062_L1095_L1.2040.57
936_T974_V1.2020.57
34_F38_S1.2010.57
1158_S1166_L1.1990.57
990_L1038_P1.1990.57
926_A948_D1.1980.57
442_G488_G1.1970.57
1116_E1220_S1.1970.57
327_A343_L1.1950.56
573_I594_A1.1950.56
355_A368_L1.1940.56
1090_A1093_Q1.1910.56
1119_R1165_G1.1910.56
1111_P1149_P1.1900.56
824_L840_V1.1900.56
1229_V1244_L1.1900.56
1081_V1265_D1.1890.56
1127_N1212_G1.1880.56
606_G630_Q1.1860.56
60_L63_V1.1840.55
559_A569_L1.1830.55
1119_R1159_Y1.1810.55
930_L945_G1.1810.55
1230_K1255_Q1.1800.55
719_C980_P1.1800.55
532_L543_I1.1800.55
971_P1243_T1.1790.55
765_G986_A1.1790.55
448_G459_N1.1790.55
928_S949_W1.1760.55
963_G1236_F1.1760.55
1206_L1215_S1.1750.54
1190_Q1193_G1.1750.54
1048_G1051_V1.1740.54
464_I467_I1.1740.54
590_A612_G1.1710.54
1159_Y1255_Q1.1700.54
573_I581_G1.1700.54
1223_I1227_L1.1700.54
993_L997_I1.1670.54
1093_Q1145_I1.1670.54
1095_L1146_F1.1670.54
806_I809_D1.1660.54
1230_K1234_G1.1660.54
25_I32_L1.1660.54
467_I472_Q1.1650.53
1252_L1259_G1.1630.53
862_L1064_G1.1620.53
1129_I1151_K1.1620.53
978_V1134_V1.1600.53
423_A432_L1.1590.53
911_P1221_G1.1590.53
1263_A1270_F1.1590.53
3_W6_R1.1570.53
1216_Q1243_T1.1570.53
1130_A1160_L1.1570.53
392_L405_L1.1550.53
1132_V1153_Q1.1520.52
1052_T1061_R1.1520.52
1062_L1259_G1.1520.52
1146_F1159_Y1.1490.52
1120_N1129_I1.1490.52
538_K541_L1.1490.52
1098_R1129_I1.1470.52
1048_G1061_R1.1460.52
1108_V1119_R1.1460.52
581_G586_P1.1450.52
85_C92_C1.1450.52
979_N998_D1.1440.51
973_M1220_S1.1440.51
857_I860_L1.1430.51
628_A641_L1.1430.51
986_A1265_D1.1420.51
1106_G1248_L1.1410.51
64_E73_N1.1410.51
883_L894_L1.1410.51
1204_L1217_V1.1410.51
1019_D1022_M1.1410.51
900_I917_G1.1390.51
1201_I1204_L1.1390.51
1067_K1113_L1.1390.51
474_P488_G1.1380.51
1230_K1233_M1.1380.51
1202_S1220_S1.1380.51
1118_I1129_I1.1350.51
728_C980_P1.1350.51
1062_L1091_Y1.1340.51
26_L30_S1.1330.50
976_I1143_V1.1330.50
1152_T1165_G1.1330.50
982_L1043_L1.1330.50
331_L339_L1.1330.50
460_A488_G1.1310.50
1215_S1265_D1.1310.50
45_G67_M1.1280.50
973_M1005_V1.1270.50
1041_S1047_I1.1270.50
1104_F1111_P1.1250.50
1217_V1248_L1.1240.50
924_N927_T1.1240.50
23_A27_G1.1230.49
1064_G1081_V1.1210.49
984_F1229_V1.1200.49
911_P971_P1.1200.49
634_A958_V1.1180.49
1192_V1196_G1.1170.49
257_G261_V1.1160.49
1231_Y1234_G1.1150.49
1146_F1231_Y1.1150.49
579_V584_N1.1150.49
321_E329_L1.1120.48
321_E327_A1.1120.48
936_T1062_L1.1120.48
1118_I1146_F1.1120.48
1099_K1140_K1.1110.48
1064_G1265_D1.1090.48
126_N139_A1.1070.48
1036_S1049_D1.1070.48
1017_S1159_Y1.1060.48
787_N814_N1.1050.48
161_W164_R1.1040.48
1216_Q1232_G1.1040.48
767_F947_A1.1040.48
25_I35_A1.1040.48
82_N94_E1.1020.47
79_L91_L1.1010.47
968_V1115_I1.0990.47
1153_Q1184_G1.0990.47
27_G30_S1.0990.47
1090_A1119_R1.0980.47
974_V1095_L1.0970.47
32_L38_S1.0970.47
76_E97_G1.0960.47
1106_G1259_G1.0940.47
187_G190_A1.0940.47
1091_Y1119_R1.0930.47
316_M323_K1.0930.47
366_L396_G1.0920.46
1098_R1148_E1.0920.46
1146_F1156_A1.0910.46
970_L1101_Q1.0910.46
1022_M1095_L1.0910.46
1233_M1240_A1.0900.46
421_L439_A1.0900.46
158_A170_P1.0890.46
922_R931_S1.0880.46
25_I30_S1.0880.46
1145_I1157_L1.0880.46
1247_R1269_Q1.0880.46
1136_G1163_G1.0880.46
1019_D1064_G1.0870.46
558_N574_K1.0860.46
1093_Q1160_L1.0860.46
1217_V1253_Y1.0850.46
1064_G1128_V1.0840.46
885_L1066_L1.0830.46
1054_D1059_K1.0820.45
840_V852_S1.0820.45
1021_V1119_R1.0810.45
1007_Q1010_P1.0810.45
731_K1021_V1.0800.45
463_T483_R1.0790.45
1157_L1162_R1.0790.45
949_W1221_G1.0780.45
631_L842_V1.0780.45
1091_Y1146_F1.0780.45
416_P497_L1.0760.45
966_L1272_F1.0760.45
1091_Y1131_G1.0750.45
643_L656_L1.0750.45
1233_M1245_R1.0750.45
1232_G1238_S1.0750.45
886_G895_F1.0750.45
1022_M1119_R1.0750.45
1090_A1131_G1.0750.45
1004_I1153_Q1.0740.45
1183_L1195_I1.0740.45
919_V986_A1.0730.45
922_R929_D1.0720.45
118_P122_L1.0720.45
941_L1197_E1.0680.44
980_P1002_A1.0670.44
879_V1115_I1.0670.44
1174_A1186_G1.0670.44
1221_G1269_Q1.0670.44
590_A1130_A1.0660.44
1091_Y1159_Y1.0650.44
648_T652_H1.0640.44
897_Q920_D1.0630.44
758_P979_N1.0630.44
589_I613_D1.0630.44
1001_W1050_D1.0630.44
1092_G1259_G1.0630.44
1112_Y1137_L1.0600.43
1247_R1255_Q1.0590.43
416_P439_A1.0590.43
372_S378_P1.0590.43
900_I1232_G1.0580.43
1099_K1116_E1.0580.43
1231_Y1235_I1.0570.43
791_Q810_A1.0560.43
631_L634_A1.0560.43
114_Q117_P1.0560.43
7_I11_L1.0560.43
1077_L1095_L1.0560.43
782_P817_I1.0550.43
1062_L1252_L1.0540.43
1243_T1255_Q1.0540.43
1019_D1132_V1.0540.43
1106_G1232_G1.0530.43
1225_D1236_F1.0530.43
423_A543_I1.0520.43
680_W1091_Y1.0510.43
1175_Q1204_L1.0510.43
968_V1207_D1.0510.43
937_P967_R1.0510.43
873_E907_G1.0500.42
974_V1159_Y1.0490.42
1217_V1264_I1.0490.42
1233_M1265_D1.0480.42
923_F1235_I1.0480.42
1132_V1145_I1.0470.42
1093_Q1246_Y1.0470.42
1156_A1159_Y1.0470.42
1196_G1231_Y1.0460.42
1166_L1185_V1.0460.42
1020_E1083_I1.0460.42
1093_Q1131_G1.0460.42
825_Q839_H1.0450.42
392_L396_G1.0450.42
1196_G1258_S1.0450.42
854_T882_Q1.0440.42
17_L21_A1.0440.42
432_L446_I1.0440.42
277_V286_A1.0440.42
541_L557_I1.0440.42
1002_A1128_V1.0440.42
995_G1149_P1.0430.42
1149_P1163_G1.0430.42
1022_M1056_F1.0430.42
1091_Y1218_V1.0430.42
555_A590_A1.0420.42
1218_V1245_R1.0420.42
1162_R1180_L1.0410.42
174_N184_P1.0410.42
286_A289_G1.0400.41
1119_R1146_F1.0400.41
900_I1238_S1.0400.41
1119_R1130_A1.0390.41
417_A439_A1.0390.41
957_A986_A1.0380.41
567_P667_L1.0370.41
782_P788_L1.0370.41
1119_R1149_P1.0370.41
719_C963_G1.0370.41
414_I439_A1.0350.41
769_G773_A1.0350.41
976_I1002_A1.0350.41
210_T216_Q1.0350.41
1156_A1243_T1.0340.41
793_V797_Q1.0330.41
1222_K1226_D1.0330.41
260_P289_G1.0320.41
458_W490_L1.0310.41
1095_L1268_Y1.0300.41
120_E124_E1.0300.41
1081_V1153_Q1.0300.41
28_T35_A1.0300.41
581_G584_N1.0300.41
961_A1102_I1.0290.40
497_L501_E1.0280.40
521_G527_W1.0280.40
100_D140_N1.0280.40
1047_I1159_Y1.0280.40
866_K1043_L1.0280.40
1056_F1255_Q1.0270.40
1022_M1111_P1.0270.40
1215_S1255_Q1.0260.40
1079_G1086_G1.0260.40
1131_G1159_Y1.0250.40
64_E71_D1.0250.40
295_G299_L1.0250.40
995_G999_I1.0240.40
1184_G1246_Y1.0240.40
277_V287_P1.0240.40
794_G807_D1.0220.40
1015_S1249_M1.0220.40
765_G1077_L1.0220.40
1056_F1259_G1.0210.40
1000_P1265_D1.0210.40
852_S885_L1.0210.40
897_Q922_R1.0200.40
515_A519_A1.0200.40
656_L727_L1.0200.40
980_P1000_P1.0200.40
517_G543_I1.0200.40
166_V180_L1.0190.40
997_I1184_G1.0190.40
769_G1165_G1.0190.40
1115_I1221_G1.0190.40
229_P232_A1.0180.39
970_L1270_F1.0170.39
1138_A1186_G1.0170.39
832_G978_V1.0160.39
764_R794_G1.0160.39
1133_K1144_E1.0140.39
1180_L1255_Q1.0140.39
680_W1090_A1.0130.39
221_T226_F1.0130.39
963_G1000_P1.0130.39
195_D201_A1.0120.39
196_V199_V1.0120.39
1159_Y1236_F1.0120.39
1110_Q1138_A1.0120.39
206_A210_T1.0120.39
917_G934_I1.0110.39
1092_G1119_R1.0100.39
539_N652_H1.0100.39
3_W14_I1.0090.39
857_I1232_G1.0090.39
1233_M1259_G1.0090.39
1108_V1111_P1.0080.39
936_T1115_I1.0080.39
1128_V1165_G1.0080.39
429_K451_D1.0070.38
28_T34_F1.0060.38
986_A1263_A1.0060.38
437_L444_A1.0060.38
1098_R1220_S1.0050.38
264_N285_D1.0050.38
1110_Q1253_Y1.0050.38
367_K395_N1.0050.38
199_V206_A1.0050.38
181_P184_P1.0030.38
442_G481_D1.0030.38
1152_T1155_E1.0030.38
995_G1019_D1.0030.38
640_Q658_M1.0030.38
869_L872_G1.0030.38
1189_G1226_D1.0030.38
717_S730_P1.0030.38
1253_Y1270_F1.0030.38
263_I286_A1.0030.38
31_G34_F1.0030.38
515_A534_L1.0020.38
484_I641_L1.0020.38
1226_D1252_L1.0020.38
1232_G1236_F1.0010.38
959_V1191_L1.0010.38
442_G474_P1.0010.38
652_H682_G1.0010.38
1128_V1192_V1.0000.38
32_L39_A1.0000.38
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (17Jul14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3lycA160.177741.50.984Contact Map
3jx8A60.185537.30.985Contact Map
4c69X10.183230.80.985Contact Map
4opwA20.184724.80.986Contact Map
3petA20.162721.30.986Contact Map
3hpeA20.116416.20.987Contact Map
1m56D20.01818.70.989Contact Map
1qleD10.01818.50.989Contact Map
1wubA10.12347.90.989Contact Map
2lmeA30.07557.10.989Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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