| ADRB2_HUMAN_LS |
| ID: | 1402944767 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 413 (357) | ||
| Sequences: | 5892 (3841) | ||
| Seq/Len: | 16.504 | ||
| Nf(neff/√len): | 203.3 |
Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 16.504).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 47_I | 83_G | 5.193 | 1.00 |
| 132_Y | 137_S | 3.444 | 1.00 |
| 74_S | 123_T | 2.894 | 1.00 |
| 99_W | 102_G | 2.875 | 1.00 |
| 104_F | 108_F | 2.827 | 1.00 |
| 217_F | 221_R | 2.820 | 1.00 |
| 126_V | 157_V | 2.755 | 1.00 |
| 64_L | 69_N | 2.691 | 1.00 |
| 58_I | 73_T | 2.672 | 1.00 |
| 186_A | 190_C | 2.592 | 1.00 |
| 74_S | 158_W | 2.445 | 1.00 |
| 70_Y | 154_I | 2.407 | 1.00 |
| 55_I | 73_T | 2.348 | 1.00 |
| 111_S | 115_L | 2.287 | 1.00 |
| 103_N | 107_E | 2.211 | 1.00 |
| 286_W | 315_G | 2.159 | 1.00 |
| 184_C | 187_N | 2.040 | 1.00 |
| 126_V | 153_I | 2.006 | 1.00 |
| 130_D | 145_L | 1.986 | 1.00 |
| 214_I | 218_V | 1.961 | 1.00 |
| 281_T | 325_I | 1.834 | 1.00 |
| 92_A | 101_F | 1.830 | 1.00 |
| 184_C | 189_T | 1.821 | 1.00 |
| 212_L | 283_T | 1.816 | 1.00 |
| 52_V | 56_T | 1.812 | 1.00 |
| 186_A | 191_C | 1.781 | 1.00 |
| 111_S | 169_I | 1.777 | 1.00 |
| 119_A | 161_S | 1.759 | 1.00 |
| 184_C | 188_E | 1.754 | 1.00 |
| 34_V | 38_I | 1.724 | 1.00 |
| 181_A | 184_C | 1.719 | 1.00 |
| 52_V | 80_L | 1.670 | 1.00 |
| 166_F | 170_Q | 1.662 | 1.00 |
| 204_S | 209_Y | 1.660 | 1.00 |
| 115_L | 162_G | 1.655 | 1.00 |
| 186_A | 189_T | 1.648 | 1.00 |
| 287_L | 291_I | 1.632 | 1.00 |
| 283_T | 287_L | 1.627 | 1.00 |
| 204_S | 294_I | 1.615 | 1.00 |
| 102_G | 105_W | 1.605 | 1.00 |
| 79_D | 322_N | 1.605 | 1.00 |
| 208_F | 287_L | 1.588 | 1.00 |
| 47_I | 320_G | 1.582 | 1.00 |
| 299_Q | 302_L | 1.574 | 1.00 |
| 31_V | 35_G | 1.560 | 1.00 |
| 289_F | 293_N | 1.557 | 1.00 |
| 129_V | 218_V | 1.552 | 1.00 |
| 124_L | 215_M | 1.546 | 1.00 |
| 148_N | 152_V | 1.542 | 1.00 |
| 56_T | 339_L | 1.535 | 1.00 |
| 221_R | 224_Q | 1.520 | 1.00 |
| 152_V | 156_M | 1.505 | 1.00 |
| 82_M | 319_S | 1.498 | 1.00 |
| 71_F | 150_A | 1.480 | 1.00 |
| 48_V | 80_L | 1.466 | 1.00 |
| 66_T | 69_N | 1.454 | 1.00 |
| 44_V | 91_A | 1.453 | 1.00 |
| 208_F | 282_F | 1.452 | 1.00 |
| 223_F | 272_L | 1.450 | 1.00 |
| 277_I | 325_I | 1.434 | 1.00 |
| 55_I | 80_L | 1.421 | 1.00 |
| 83_G | 319_S | 1.418 | 1.00 |
| 123_T | 154_I | 1.409 | 1.00 |
| 201_I | 298_I | 1.397 | 0.99 |
| 198_A | 202_A | 1.389 | 0.99 |
| 58_I | 64_L | 1.380 | 0.99 |
| 132_Y | 221_R | 1.371 | 0.99 |
| 50_G | 76_A | 1.371 | 0.99 |
| 224_Q | 228_R | 1.367 | 0.99 |
| 268_E | 272_L | 1.360 | 0.99 |
| 197_Q | 298_I | 1.344 | 0.99 |
| 220_S | 224_Q | 1.340 | 0.99 |
| 86_V | 90_G | 1.331 | 0.99 |
| 120_S | 318_N | 1.325 | 0.99 |
| 317_V | 321_F | 1.322 | 0.99 |
| 37_G | 95_L | 1.315 | 0.99 |
| 170_Q | 173_W | 1.304 | 0.99 |
| 229_Q | 233_I | 1.299 | 0.99 |
| 125_C | 214_I | 1.288 | 0.99 |
| 134_A | 138_P | 1.279 | 0.99 |
| 48_V | 83_G | 1.266 | 0.99 |
| 216_V | 220_S | 1.260 | 0.99 |
| 37_G | 91_A | 1.255 | 0.99 |
| 50_G | 322_N | 1.251 | 0.99 |
| 61_F | 335_A | 1.248 | 0.99 |
| 132_Y | 136_T | 1.237 | 0.98 |
| 44_V | 83_G | 1.221 | 0.98 |
| 74_S | 154_I | 1.218 | 0.98 |
| 73_T | 77_C | 1.216 | 0.98 |
| 54_V | 58_I | 1.210 | 0.98 |
| 363_Q | 366_Y | 1.209 | 0.98 |
| 71_F | 145_L | 1.208 | 0.98 |
| 133_F | 137_S | 1.208 | 0.98 |
| 278_I | 325_I | 1.202 | 0.98 |
| 54_V | 323_P | 1.201 | 0.98 |
| 113_D | 316_Y | 1.193 | 0.98 |
| 76_A | 322_N | 1.193 | 0.98 |
| 291_I | 295_V | 1.192 | 0.98 |
| 137_S | 140_K | 1.192 | 0.98 |
| 30_E | 34_V | 1.190 | 0.98 |
| 50_G | 320_G | 1.190 | 0.98 |
| 285_C | 321_F | 1.180 | 0.98 |
| 46_A | 320_G | 1.180 | 0.98 |
| 153_I | 157_V | 1.178 | 0.98 |
| 223_F | 227_K | 1.174 | 0.98 |
| 125_C | 218_V | 1.174 | 0.98 |
| 111_S | 162_G | 1.166 | 0.97 |
| 118_T | 207_S | 1.152 | 0.97 |
| 226_A | 269_H | 1.151 | 0.97 |
| 307_V | 311_L | 1.145 | 0.97 |
| 289_F | 315_G | 1.144 | 0.97 |
| 289_F | 312_N | 1.135 | 0.97 |
| 133_F | 144_L | 1.135 | 0.97 |
| 78_A | 120_S | 1.134 | 0.97 |
| 286_W | 322_N | 1.134 | 0.97 |
| 54_V | 327_C | 1.133 | 0.97 |
| 155_L | 159_I | 1.133 | 0.97 |
| 122_E | 160_V | 1.124 | 0.97 |
| 144_L | 149_K | 1.119 | 0.96 |
| 221_R | 225_E | 1.119 | 0.96 |
| 208_F | 285_C | 1.118 | 0.96 |
| 202_A | 206_V | 1.112 | 0.96 |
| 141_Y | 226_A | 1.112 | 0.96 |
| 227_K | 231_Q | 1.110 | 0.96 |
| 89_F | 101_F | 1.108 | 0.96 |
| 152_V | 155_L | 1.107 | 0.96 |
| 132_Y | 217_F | 1.105 | 0.96 |
| 55_I | 77_C | 1.098 | 0.96 |
| 37_G | 41_S | 1.096 | 0.96 |
| 292_V | 311_L | 1.095 | 0.96 |
| 158_W | 323_P | 1.095 | 0.96 |
| 89_F | 105_W | 1.092 | 0.96 |
| 130_D | 271_A | 1.091 | 0.96 |
| 64_L | 336_F | 1.089 | 0.96 |
| 36_M | 40_M | 1.087 | 0.96 |
| 55_I | 76_A | 1.086 | 0.96 |
| 226_A | 229_Q | 1.070 | 0.95 |
| 223_F | 226_A | 1.069 | 0.95 |
| 58_I | 65_Q | 1.067 | 0.95 |
| 76_A | 79_D | 1.067 | 0.95 |
| 215_M | 279_M | 1.067 | 0.95 |
| 78_A | 318_N | 1.066 | 0.95 |
| 361_G | 364_S | 1.061 | 0.95 |
| 208_F | 283_T | 1.059 | 0.95 |
| 89_F | 109_W | 1.054 | 0.95 |
| 46_A | 49_F | 1.053 | 0.95 |
| 121_I | 282_F | 1.051 | 0.94 |
| 145_L | 149_K | 1.051 | 0.94 |
| 290_F | 315_G | 1.039 | 0.94 |
| 362_E | 365_G | 1.039 | 0.94 |
| 198_A | 201_I | 1.033 | 0.94 |
| 168_P | 172_H | 1.032 | 0.94 |
| 337_Q | 341_C | 1.027 | 0.94 |
| 76_A | 332_F | 1.025 | 0.93 |
| 352_N | 356_S | 1.023 | 0.93 |
| 146_T | 149_K | 1.022 | 0.93 |
| 113_D | 117_V | 1.021 | 0.93 |
| 208_F | 212_L | 1.019 | 0.93 |
| 105_W | 108_F | 1.019 | 0.93 |
| 207_S | 211_P | 1.017 | 0.93 |
| 281_T | 321_F | 1.011 | 0.93 |
| 141_Y | 145_L | 1.008 | 0.93 |
| 114_V | 165_S | 1.006 | 0.93 |
| 90_G | 312_N | 1.004 | 0.93 |
| 54_V | 322_N | 1.003 | 0.93 |
| 121_I | 290_F | 1.002 | 0.92 |
| 171_M | 174_Y | 1.001 | 0.92 |
| 225_E | 228_R | 1.001 | 0.92 |
| 119_A | 123_T | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2r4rA | 1 | 0.523 | 100 | 0.284 | Contact Map |
| 2rh1A | 1 | 0.7603 | 100 | 0.325 | Contact Map |
| 4iarA | 1 | 0.7409 | 100 | 0.355 | Contact Map |
| 4bvnA | 1 | 0.6998 | 100 | 0.356 | Contact Map |
| 4pxzA | 1 | 0.7264 | 100 | 0.378 | Contact Map |
| 3uonA | 1 | 0.7409 | 100 | 0.38 | Contact Map |
| 4grvA | 1 | 0.7482 | 100 | 0.382 | Contact Map |
| 4mbsA | 2 | 0.7676 | 100 | 0.388 | Contact Map |
| 3vw7A | 1 | 0.7312 | 100 | 0.389 | Contact Map |
| 4dklA | 1 | 0.7506 | 100 | 0.393 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)