| Hsc70_homo |
| ID: | 1402325012 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 646 (636) | ||
| Sequences: | 5349 (2776.4) | ||
| Seq/Len: | 8.410 | ||
| Nf(neff/√len): | 110.1 |
Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 8.410).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 225_D | 312_G | 6.275 | 1.00 |
| 141_N | 168_N | 5.218 | 1.00 |
| 236_R | 308_D | 4.561 | 1.00 |
| 237_M | 297_I | 4.180 | 1.00 |
| 69_D | 231_E | 3.751 | 1.00 |
| 198_F | 313_T | 3.583 | 1.00 |
| 351_Q | 358_E | 3.473 | 1.00 |
| 242_I | 254_S | 3.450 | 1.00 |
| 209_I | 320_A | 3.394 | 1.00 |
| 221_S | 320_A | 3.175 | 1.00 |
| 400_S | 412_V | 3.140 | 1.00 |
| 31_N | 38_T | 3.110 | 1.00 |
| 348_K | 352_D | 3.087 | 1.00 |
| 279_Q | 296_S | 2.958 | 1.00 |
| 441_Q | 455_L | 2.793 | 1.00 |
| 244_E | 301_R | 2.789 | 1.00 |
| 439_L | 460_E | 2.739 | 1.00 |
| 196_L | 207_V | 2.679 | 1.00 |
| 364_N | 367_E | 2.677 | 1.00 |
| 550_K | 578_I | 2.597 | 1.00 |
| 525_Y | 528_E | 2.568 | 1.00 |
| 141_N | 170_L | 2.566 | 1.00 |
| 6_A | 170_L | 2.561 | 1.00 |
| 512_K | 516_E | 2.547 | 1.00 |
| 412_V | 415_K | 2.485 | 1.00 |
| 241_F | 295_T | 2.464 | 1.00 |
| 340_S | 343_I | 2.442 | 1.00 |
| 347_Q | 361_K | 2.437 | 1.00 |
| 523_E | 526_K | 2.364 | 1.00 |
| 200_L | 233_F | 2.345 | 1.00 |
| 411_T | 428_F | 2.295 | 1.00 |
| 568_Q | 572_D | 2.253 | 1.00 |
| 302_F | 340_S | 2.251 | 1.00 |
| 128_K | 132_E | 2.249 | 1.00 |
| 241_F | 267_C | 2.222 | 1.00 |
| 301_R | 304_E | 2.197 | 1.00 |
| 306_N | 343_I | 2.172 | 1.00 |
| 443_Y | 455_L | 2.154 | 1.00 |
| 233_F | 309_L | 2.132 | 1.00 |
| 196_L | 209_I | 2.122 | 1.00 |
| 49_R | 122_M | 2.106 | 1.00 |
| 129_E | 133_A | 2.102 | 1.00 |
| 302_F | 343_I | 2.074 | 1.00 |
| 349_L | 352_D | 2.069 | 1.00 |
| 402_G | 410_M | 2.066 | 1.00 |
| 315_D | 319_K | 2.062 | 1.00 |
| 310_F | 345_K | 2.061 | 1.00 |
| 593_E | 597_K | 2.058 | 1.00 |
| 491_V | 498_E | 2.055 | 1.00 |
| 35_N | 53_D | 2.043 | 1.00 |
| 7_V | 18_V | 2.030 | 1.00 |
| 79_D | 96_N | 2.024 | 1.00 |
| 314_L | 353_F | 2.018 | 1.00 |
| 579_N | 582_D | 1.970 | 1.00 |
| 244_E | 247_R | 1.958 | 1.00 |
| 517_R | 521_E | 1.952 | 1.00 |
| 238_V | 264_R | 1.948 | 1.00 |
| 281_S | 294_Y | 1.941 | 1.00 |
| 608_T | 612_Q | 1.934 | 1.00 |
| 575_N | 579_N | 1.928 | 1.00 |
| 530_E | 534_D | 1.916 | 1.00 |
| 563_N | 566_D | 1.914 | 1.00 |
| 163_T | 512_K | 1.913 | 1.00 |
| 589_K | 593_E | 1.900 | 1.00 |
| 542_L | 577_I | 1.889 | 1.00 |
| 235_N | 239_N | 1.888 | 1.00 |
| 527_A | 531_K | 1.861 | 1.00 |
| 6_A | 377_A | 1.857 | 1.00 |
| 571_L | 575_N | 1.855 | 1.00 |
| 566_D | 609_K | 1.854 | 1.00 |
| 64_T | 93_M | 1.850 | 1.00 |
| 145_T | 373_A | 1.825 | 1.00 |
| 559_Q | 567_K | 1.823 | 1.00 |
| 127_M | 130_I | 1.801 | 1.00 |
| 106_E | 111_T | 1.799 | 1.00 |
| 315_D | 318_E | 1.787 | 1.00 |
| 19_G | 374_A | 1.757 | 1.00 |
| 595_Q | 598_E | 1.743 | 1.00 |
| 307_A | 311_R | 1.739 | 1.00 |
| 394_L | 419_T | 1.738 | 1.00 |
| 585_Q | 591_E | 1.732 | 1.00 |
| 516_E | 520_Q | 1.730 | 1.00 |
| 605_P | 608_T | 1.717 | 1.00 |
| 17_C | 28_I | 1.708 | 1.00 |
| 266_A | 270_A | 1.706 | 1.00 |
| 163_T | 168_N | 1.698 | 1.00 |
| 17_C | 37_T | 1.685 | 1.00 |
| 449_M | 536_V | 1.679 | 1.00 |
| 241_F | 263_L | 1.658 | 1.00 |
| 209_I | 331_I | 1.652 | 1.00 |
| 268_E | 272_R | 1.635 | 1.00 |
| 520_Q | 523_E | 1.626 | 1.00 |
| 64_T | 257_K | 1.625 | 1.00 |
| 576_E | 595_Q | 1.624 | 1.00 |
| 521_E | 524_K | 1.606 | 1.00 |
| 243_A | 254_S | 1.604 | 1.00 |
| 156_Q | 160_D | 1.604 | 1.00 |
| 282_I | 297_I | 1.603 | 1.00 |
| 441_Q | 458_K | 1.600 | 1.00 |
| 342_R | 366_D | 1.589 | 1.00 |
| 597_K | 601_K | 1.581 | 1.00 |
| 314_L | 318_E | 1.576 | 1.00 |
| 318_E | 353_F | 1.572 | 1.00 |
| 270_A | 282_I | 1.571 | 1.00 |
| 237_M | 267_C | 1.567 | 1.00 |
| 114_F | 119_V | 1.560 | 1.00 |
| 564_D | 568_Q | 1.553 | 1.00 |
| 449_M | 452_D | 1.552 | 1.00 |
| 520_Q | 524_K | 1.549 | 1.00 |
| 44_F | 122_M | 1.547 | 1.00 |
| 549_M | 603_C | 1.545 | 1.00 |
| 412_V | 448_A | 1.540 | 1.00 |
| 22_Q | 27_E | 1.539 | 1.00 |
| 573_K | 598_E | 1.532 | 1.00 |
| 333_D | 360_N | 1.530 | 1.00 |
| 126_K | 130_I | 1.524 | 1.00 |
| 327_D | 330_Q | 1.522 | 1.00 |
| 120_S | 158_T | 1.521 | 1.00 |
| 572_D | 576_E | 1.517 | 1.00 |
| 4_G | 141_N | 1.509 | 1.00 |
| 439_L | 458_K | 1.502 | 1.00 |
| 65_N | 110_E | 1.499 | 1.00 |
| 162_G | 172_I | 1.497 | 1.00 |
| 306_N | 310_F | 1.493 | 1.00 |
| 253_I | 287_L | 1.487 | 1.00 |
| 249_H | 291_I | 1.486 | 1.00 |
| 118_E | 122_M | 1.480 | 1.00 |
| 603_C | 607_I | 1.477 | 1.00 |
| 546_A | 578_I | 1.476 | 1.00 |
| 301_R | 305_L | 1.475 | 1.00 |
| 193_R | 333_D | 1.462 | 0.99 |
| 270_A | 280_A | 1.459 | 0.99 |
| 473_Q | 493_K | 1.450 | 0.99 |
| 600_E | 604_N | 1.448 | 0.99 |
| 241_F | 282_I | 1.439 | 0.99 |
| 29_I | 134_Y | 1.435 | 0.99 |
| 425_T | 477_T | 1.430 | 0.99 |
| 318_E | 322_R | 1.429 | 0.99 |
| 70_A | 123_V | 1.428 | 0.99 |
| 36_R | 367_E | 1.423 | 0.99 |
| 200_L | 205_F | 1.422 | 0.99 |
| 397_T | 480_I | 1.415 | 0.99 |
| 84_Q | 87_M | 1.410 | 0.99 |
| 98_A | 101_P | 1.409 | 0.99 |
| 319_K | 322_R | 1.398 | 0.99 |
| 304_E | 307_A | 1.393 | 0.99 |
| 220_K | 326_L | 1.386 | 0.99 |
| 240_H | 301_R | 1.375 | 0.99 |
| 233_F | 302_F | 1.375 | 0.99 |
| 303_E | 348_K | 1.375 | 0.99 |
| 209_I | 326_L | 1.374 | 0.99 |
| 117_E | 160_D | 1.370 | 0.99 |
| 122_M | 125_T | 1.368 | 0.99 |
| 350_L | 359_L | 1.366 | 0.99 |
| 546_A | 577_I | 1.360 | 0.99 |
| 43_A | 58_Q | 1.358 | 0.99 |
| 68_F | 91_P | 1.343 | 0.99 |
| 19_G | 370_A | 1.340 | 0.99 |
| 585_Q | 588_E | 1.339 | 0.99 |
| 526_K | 530_E | 1.338 | 0.99 |
| 194_N | 220_K | 1.335 | 0.99 |
| 443_Y | 450_T | 1.330 | 0.99 |
| 465_P | 494_S | 1.324 | 0.99 |
| 564_D | 571_L | 1.323 | 0.99 |
| 16_S | 127_M | 1.322 | 0.99 |
| 410_M | 443_Y | 1.320 | 0.99 |
| 573_K | 576_E | 1.316 | 0.99 |
| 11_L | 158_T | 1.312 | 0.99 |
| 236_R | 306_N | 1.311 | 0.99 |
| 233_F | 343_I | 1.304 | 0.98 |
| 240_H | 297_I | 1.304 | 0.98 |
| 298_T | 301_R | 1.303 | 0.98 |
| 425_T | 475_E | 1.300 | 0.98 |
| 609_K | 612_Q | 1.297 | 0.98 |
| 455_L | 458_K | 1.294 | 0.98 |
| 160_D | 163_T | 1.292 | 0.98 |
| 196_L | 317_V | 1.291 | 0.98 |
| 591_E | 595_Q | 1.291 | 0.98 |
| 345_K | 348_K | 1.286 | 0.98 |
| 319_K | 323_D | 1.284 | 0.98 |
| 534_D | 589_K | 1.283 | 0.98 |
| 58_Q | 62_N | 1.272 | 0.98 |
| 244_E | 295_T | 1.269 | 0.98 |
| 246_K | 254_S | 1.259 | 0.98 |
| 405_T | 411_T | 1.256 | 0.98 |
| 67_V | 119_V | 1.256 | 0.98 |
| 541_S | 596_Q | 1.251 | 0.98 |
| 604_N | 607_I | 1.251 | 0.98 |
| 314_L | 349_L | 1.245 | 0.98 |
| 28_I | 371_Y | 1.240 | 0.98 |
| 246_K | 252_D | 1.240 | 0.98 |
| 567_K | 571_L | 1.239 | 0.98 |
| 223_A | 316_P | 1.232 | 0.98 |
| 538_S | 593_E | 1.227 | 0.98 |
| 297_I | 302_F | 1.224 | 0.97 |
| 590_E | 594_H | 1.222 | 0.97 |
| 70_A | 120_S | 1.222 | 0.97 |
| 11_L | 124_L | 1.220 | 0.97 |
| 21_F | 378_A | 1.218 | 0.97 |
| 573_K | 577_I | 1.216 | 0.97 |
| 511_S | 514_D | 1.215 | 0.97 |
| 348_K | 351_Q | 1.213 | 0.97 |
| 546_A | 574_C | 1.209 | 0.97 |
| 240_H | 295_T | 1.208 | 0.97 |
| 106_E | 113_S | 1.202 | 0.97 |
| 194_N | 326_L | 1.201 | 0.97 |
| 321_L | 328_K | 1.195 | 0.97 |
| 13_T | 203_G | 1.192 | 0.97 |
| 78_F | 84_Q | 1.190 | 0.97 |
| 239_N | 254_S | 1.188 | 0.97 |
| 588_E | 591_E | 1.186 | 0.97 |
| 538_S | 596_Q | 1.184 | 0.97 |
| 607_I | 611_Y | 1.183 | 0.97 |
| 237_M | 240_H | 1.182 | 0.97 |
| 266_A | 280_A | 1.176 | 0.97 |
| 326_L | 330_Q | 1.173 | 0.96 |
| 70_A | 119_V | 1.172 | 0.96 |
| 44_F | 114_F | 1.171 | 0.96 |
| 170_L | 380_L | 1.171 | 0.96 |
| 174_N | 395_D | 1.170 | 0.96 |
| 566_D | 569_K | 1.167 | 0.96 |
| 518_M | 521_E | 1.166 | 0.96 |
| 32_D | 130_I | 1.159 | 0.96 |
| 467_A | 492_D | 1.154 | 0.96 |
| 5_P | 137_K | 1.143 | 0.96 |
| 594_H | 598_E | 1.143 | 0.96 |
| 194_N | 211_T | 1.143 | 0.96 |
| 635_G | 638_S | 1.140 | 0.96 |
| 320_A | 324_A | 1.130 | 0.95 |
| 273_T | 280_A | 1.128 | 0.95 |
| 576_E | 598_E | 1.126 | 0.95 |
| 411_T | 426_Q | 1.126 | 0.95 |
| 144_V | 162_G | 1.126 | 0.95 |
| 310_F | 349_L | 1.125 | 0.95 |
| 128_K | 167_L | 1.125 | 0.95 |
| 283_E | 294_Y | 1.121 | 0.95 |
| 104_Q | 111_T | 1.120 | 0.95 |
| 18_V | 127_M | 1.118 | 0.95 |
| 205_F | 313_T | 1.117 | 0.95 |
| 14_T | 202_G | 1.110 | 0.95 |
| 533_R | 537_S | 1.109 | 0.95 |
| 233_F | 306_N | 1.108 | 0.95 |
| 317_V | 350_L | 1.106 | 0.95 |
| 223_A | 319_K | 1.105 | 0.95 |
| 549_M | 570_I | 1.103 | 0.95 |
| 240_H | 305_L | 1.100 | 0.94 |
| 96_N | 99_G | 1.100 | 0.94 |
| 233_F | 310_F | 1.099 | 0.94 |
| 30_A | 134_Y | 1.094 | 0.94 |
| 159_K | 485_I | 1.091 | 0.94 |
| 319_K | 416_R | 1.087 | 0.94 |
| 299_R | 303_E | 1.082 | 0.94 |
| 423_K | 477_T | 1.079 | 0.94 |
| 528_E | 531_K | 1.078 | 0.94 |
| 306_N | 345_K | 1.078 | 0.94 |
| 239_N | 243_A | 1.078 | 0.94 |
| 236_R | 309_L | 1.076 | 0.94 |
| 596_Q | 600_E | 1.075 | 0.94 |
| 540_N | 547_F | 1.074 | 0.94 |
| 87_M | 94_V | 1.073 | 0.93 |
| 550_K | 554_E | 1.070 | 0.93 |
| 558_L | 610_L | 1.068 | 0.93 |
| 36_R | 364_N | 1.067 | 0.93 |
| 539_K | 581_L | 1.067 | 0.93 |
| 41_Y | 68_F | 1.063 | 0.93 |
| 418_T | 422_T | 1.056 | 0.93 |
| 86_D | 227_H | 1.055 | 0.93 |
| 605_P | 609_K | 1.054 | 0.93 |
| 62_N | 65_N | 1.053 | 0.93 |
| 42_V | 119_V | 1.052 | 0.93 |
| 594_H | 597_K | 1.049 | 0.92 |
| 345_K | 349_L | 1.048 | 0.92 |
| 121_S | 164_I | 1.048 | 0.92 |
| 11_L | 120_S | 1.047 | 0.92 |
| 245_F | 293_F | 1.044 | 0.92 |
| 447_R | 529_D | 1.042 | 0.92 |
| 84_Q | 88_K | 1.039 | 0.92 |
| 79_D | 99_G | 1.036 | 0.92 |
| 93_M | 106_E | 1.035 | 0.92 |
| 176_P | 206_D | 1.029 | 0.91 |
| 196_L | 354_F | 1.029 | 0.91 |
| 170_L | 377_A | 1.028 | 0.91 |
| 104_Q | 113_S | 1.027 | 0.91 |
| 200_L | 309_L | 1.026 | 0.91 |
| 549_M | 574_C | 1.025 | 0.91 |
| 87_M | 93_M | 1.025 | 0.91 |
| 151_N | 225_D | 1.024 | 0.91 |
| 307_A | 345_K | 1.023 | 0.91 |
| 10_D | 13_T | 1.019 | 0.91 |
| 243_A | 246_K | 1.019 | 0.91 |
| 8_G | 12_G | 1.019 | 0.91 |
| 43_A | 50_L | 1.016 | 0.91 |
| 527_A | 530_E | 1.015 | 0.91 |
| 241_F | 297_I | 1.009 | 0.90 |
| 619_G | 622_P | 1.009 | 0.90 |
| 31_N | 36_R | 1.007 | 0.90 |
| 16_S | 40_S | 1.005 | 0.90 |
| 242_I | 263_L | 1.005 | 0.90 |
| 323_D | 416_R | 1.005 | 0.90 |
| 616_G | 619_G | 1.004 | 0.90 |
| 617_M | 620_G | 1.001 | 0.90 |
| 19_G | 28_I | 1.001 | 0.90 |
| 130_I | 133_A | 1.001 | 0.90 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3d2fA | 2 | 0.9443 | 100 | 0.176 | Contact Map |
| 4jneA | 2 | 0.9149 | 100 | 0.223 | Contact Map |
| 4fl9A | 1 | 0.8344 | 100 | 0.234 | Contact Map |
| 2v7yA | 1 | 0.774 | 100 | 0.334 | Contact Map |
| 4j8fA | 1 | 0.8421 | 100 | 0.465 | Contact Map |
| 3i33A | 1 | 0.5851 | 100 | 0.491 | Contact Map |
| 3qfuA | 1 | 0.5851 | 100 | 0.495 | Contact Map |
| 4kboA | 1 | 0.5728 | 100 | 0.507 | Contact Map |
| 4gniA | 2 | 0.5743 | 100 | 0.529 | Contact Map |
| 1jceA | 1 | 0.4938 | 100 | 0.684 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)