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OPENSEQ.org

Moesin

ID: 1401907826 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 577 (566)
Sequences: 674 (448.9)
Seq/Len: 1.191
Nf(neff/√len): 18.9

Jackhmmer Results: (2014_04_16)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.191).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
146_Y176_H2.8271.00
445_W448_K1.8350.94
431_L445_W1.7660.92
136_D181_G1.6920.90
27_K31_D1.6110.87
338_E342_R1.5920.86
332_M335_K1.5850.86
432_E449_A1.5750.85
171_R285_M1.5650.85
442_A445_W1.5520.84
102_F107_K1.5510.84
338_E341_E1.5220.82
423_E432_E1.4740.80
424_L431_L1.4720.79
474_E477_E1.4520.78
423_E431_L1.4430.78
424_L445_W1.4300.77
490_A493_D1.4170.76
341_E345_E1.4140.75
325_N328_K1.3970.74
490_A494_L1.3900.74
426_A430_Q1.3900.74
399_A402_A1.3900.74
424_L448_K1.3890.74
58_W68_Q1.3820.73
425_T432_E1.3790.73
14_A23_N1.3690.72
467_M470_P1.3650.72
409_D413_T1.3580.71
428_I448_K1.3500.71
127_S179_H1.3480.70
256_V274_L1.3380.70
124_L179_H1.3350.69
131_Q136_D1.3300.69
509_T516_E1.3240.68
281_L290_L1.3200.68
444_E448_K1.3140.68
130_V186_D1.3000.66
428_I431_L1.2910.66
473_A477_E1.2890.65
424_L428_I1.2860.65
374_E379_R1.2860.65
453_Q456_L1.2820.65
372_A382_A1.2820.65
477_E492_A1.2740.64
413_T418_A1.2730.64
383_Q387_E1.2720.64
418_A435_R1.2690.64
479_E489_E1.2670.63
418_A424_L1.2670.63
428_I433_M1.2650.63
431_L448_K1.2540.62
471_H477_E1.2500.62
503_R510_T1.2470.62
431_L435_R1.2440.61
445_W452_V1.2400.61
409_D412_K1.2400.61
146_Y173_Q1.2400.61
432_E445_W1.2390.61
489_E497_D1.2380.61
422_A425_T1.2360.61
495_R500_A1.2360.61
375_L384_S1.2350.61
405_Q410_Q1.2330.60
124_L249_S1.2280.60
425_T446_Q1.2270.60
421_M424_L1.2240.60
428_I445_W1.2220.59
410_Q415_E1.2220.59
433_M447_Q1.2190.59
59_L65_V1.2160.59
428_I446_Q1.2140.59
404_L408_R1.2130.58
563_R566_N1.2130.58
466_A471_H1.2120.58
380_K384_S1.2100.58
378_E382_A1.2090.58
441_E453_Q1.2060.58
475_P491_S1.2040.58
425_T449_A1.2030.58
424_L432_E1.2020.57
374_E378_E1.1990.57
147_L168_W1.1980.57
442_A447_Q1.1960.57
414_Q430_Q1.1960.57
435_R442_A1.1950.57
23_N307_A1.1940.57
330_R334_E1.1940.57
448_K451_M1.1920.56
7_V177_E1.1910.56
419_L432_E1.1900.56
431_L434_A1.1880.56
410_Q413_T1.1870.56
394_Q397_E1.1860.56
445_W450_Q1.1860.56
526_T529_L1.1850.56
349_E352_K1.1800.55
428_I432_E1.1790.55
495_R498_A1.1790.55
426_A445_W1.1770.55
441_E445_W1.1750.55
339_K342_R1.1740.55
315_Q318_M1.1720.55
109_G113_D1.1720.55
372_A376_E1.1700.54
286_G290_L1.1680.54
391_K398_E1.1670.54
435_R441_E1.1640.54
464_K467_M1.1640.54
492_A498_A1.1640.54
415_E420_E1.1630.54
442_A446_Q1.1610.53
361_A377_Q1.1600.53
375_L385_E1.1600.53
338_E343_E1.1570.53
400_K403_L1.1560.53
384_S396_A1.1540.53
15_E31_D1.1530.53
106_V191_Y1.1530.53
487_G491_S1.1520.53
491_S498_A1.1520.53
429_S445_W1.1520.53
370_R374_E1.1520.53
400_K404_L1.1490.52
479_E491_S1.1480.52
382_A390_A1.1470.52
504_S507_E1.1450.52
30_F172_I1.1450.52
506_E510_T1.1440.52
361_A365_L1.1400.51
503_R511_E1.1380.51
315_Q320_R1.1380.51
480_Q484_D1.1380.51
436_Q445_W1.1340.51
485_E498_A1.1330.51
395_E402_A1.1320.51
424_L430_Q1.1310.51
407_S410_Q1.1290.50
530_A533_R1.1280.50
466_A470_P1.1270.50
480_Q483_Q1.1270.50
429_S442_A1.1270.50
445_W449_A1.1260.50
75_P296_K1.1260.50
59_L63_K1.1260.50
530_A536_S1.1240.50
423_E426_A1.1220.50
410_Q414_Q1.1210.50
427_R442_A1.1210.50
429_S434_A1.1210.50
30_F334_E1.1200.50
375_L383_Q1.1190.49
511_E515_N1.1190.49
196_Q204_N1.1190.49
386_A390_A1.1180.49
478_N489_E1.1180.49
421_M428_I1.1180.49
14_A152_L1.1180.49
424_L427_R1.1180.49
435_R445_W1.1170.49
445_W451_M1.1160.49
414_Q424_L1.1150.49
436_Q448_K1.1140.49
493_D498_A1.1140.49
436_Q441_E1.1130.49
529_L533_R1.1120.49
470_P474_E1.1110.49
234_L244_E1.1080.48
470_P473_A1.1060.48
209_K213_G1.1050.48
509_T512_A1.1040.48
441_E452_V1.1040.48
425_T447_Q1.1000.48
450_Q453_Q1.0980.47
448_K452_V1.0980.47
494_L497_D1.0960.47
318_M321_A1.0950.47
211_K254_K1.0940.47
360_K364_E1.0930.47
449_A452_V1.0890.47
399_A407_S1.0880.46
487_G490_A1.0870.46
340_I343_E1.0870.46
371_R378_E1.0870.46
102_F250_F1.0850.46
467_M473_A1.0830.46
486_N489_E1.0830.46
492_A497_D1.0810.46
124_L127_S1.0810.46
449_A453_Q1.0800.46
418_A422_A1.0800.46
489_E493_D1.0790.46
434_A445_W1.0780.45
398_E401_E1.0780.45
440_S445_W1.0760.45
474_E478_N1.0750.45
436_Q443_V1.0740.45
168_W172_I1.0720.45
470_P493_D1.0700.45
467_M471_H1.0690.45
475_P490_A1.0690.45
491_S501_K1.0690.45
426_A432_E1.0680.44
427_R430_Q1.0670.44
359_K363_Q1.0670.44
478_N497_D1.0650.44
526_T532_A1.0650.44
423_E427_R1.0650.44
379_R396_A1.0650.44
378_E381_R1.0640.44
77_L179_H1.0630.44
438_K445_W1.0620.44
511_E517_R1.0620.44
481_D497_D1.0610.44
443_V453_Q1.0610.44
364_E368_Q1.0610.44
420_E428_I1.0610.44
425_T428_I1.0600.44
427_R434_A1.0600.44
107_K122_A1.0580.44
437_K447_Q1.0580.44
367_E370_R1.0570.43
402_A408_R1.0570.43
375_L378_E1.0560.43
367_E371_R1.0550.43
475_P478_N1.0540.43
460_R470_P1.0520.43
314_H317_Q1.0510.43
471_H492_A1.0510.43
429_S444_E1.0500.43
396_A410_Q1.0500.43
432_E435_R1.0490.43
496_A500_A1.0490.43
477_E493_D1.0480.43
246_R250_F1.0470.42
221_D239_G1.0470.42
429_S433_M1.0460.42
250_F268_V1.0450.42
492_A499_M1.0450.42
350_R357_Q1.0420.42
518_V521_H1.0410.42
203_V219_G1.0410.42
406_A410_Q1.0410.42
408_R411_K1.0400.42
386_A392_E1.0400.42
405_Q411_K1.0390.42
362_Q369_T1.0380.42
504_S509_T1.0370.42
440_S448_K1.0360.41
130_V146_Y1.0350.41
430_Q437_K1.0350.41
436_Q440_S1.0340.41
404_L410_Q1.0340.41
278_K288_H1.0310.41
437_K444_E1.0300.41
481_D499_M1.0300.41
327_K336_E1.0300.41
514_K517_R1.0290.41
473_A476_A1.0290.41
316_K321_A1.0280.41
424_L435_R1.0280.41
325_N332_M1.0270.41
122_A245_I1.0270.41
356_E359_K1.0270.41
341_E344_K1.0270.41
421_M425_T1.0270.41
388_K392_E1.0270.41
438_K441_E1.0260.40
344_K347_L1.0260.40
349_E357_Q1.0250.40
120_E124_L1.0250.40
424_L433_M1.0240.40
496_A499_M1.0230.40
425_T448_K1.0230.40
413_T425_T1.0230.40
14_A108_E1.0230.40
216_L221_D1.0220.40
442_A453_Q1.0220.40
399_A403_L1.0210.40
408_R412_K1.0210.40
392_E398_E1.0200.40
444_E451_M1.0200.40
394_Q402_A1.0200.40
132_S222_A1.0190.40
411_K414_Q1.0190.40
491_S500_A1.0180.40
361_A369_T1.0180.40
431_L440_S1.0170.40
220_V249_S1.0160.40
227_I285_M1.0160.40
249_S284_C1.0150.39
373_L398_E1.0140.39
405_Q413_T1.0140.39
434_A451_M1.0130.39
441_E444_E1.0130.39
371_R374_E1.0110.39
489_E492_A1.0110.39
102_F199_E1.0110.39
416_Q424_L1.0100.39
477_E489_E1.0100.39
316_K320_R1.0100.39
503_R514_K1.0090.39
478_N481_D1.0090.39
459_T462_E1.0090.39
524_A530_A1.0090.39
348_M351_L1.0080.39
460_R466_A1.0080.39
456_L460_R1.0080.39
148_A169_E1.0080.39
442_A451_M1.0080.39
446_Q449_A1.0070.39
509_T513_E1.0070.39
510_T515_N1.0070.39
382_A386_A1.0060.39
371_R379_R1.0060.39
351_L355_E1.0050.39
342_R346_E1.0050.39
412_K424_L1.0050.39
313_K317_Q1.0040.38
361_A368_Q1.0040.38
525_L528_E1.0040.38
527_S535_E1.0030.38
468_S497_D1.0030.38
350_R355_E1.0020.38
250_F253_K1.0020.38
562_I566_N1.0010.38
132_S223_L1.0000.38
357_Q361_A1.0000.38
413_T435_R1.0000.38
504_S513_E1.0000.38
426_A429_S1.0000.38
339_K343_E1.0000.38
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (29May14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2i1jA10.82671000.262Contact Map
1h4rA20.50951000.502Contact Map
3qijA20.47491000.506Contact Map
1ef1A20.50951000.507Contact Map
2al6A20.55291000.592Contact Map
4ekuA10.5131000.608Contact Map
3ivfA10.47141000.614Contact Map
3au4A10.49221000.627Contact Map
4hdoA10.48351000.642Contact Map
3pvlA10.60831000.643Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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