| Q8IWU4 ZNT8_HUMAN |
| ID: | 1396483829 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 369 (294) | ||
| Sequences: | 2439 (1785.5) | ||
| Seq/Len: | 8.296 | ||
| Nf(neff/√len): | 104.1 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 8.296).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 246_I | 250_I | 4.024 | 1.00 |
| 301_H | 318_H | 3.485 | 1.00 |
| 317_A | 349_I | 2.752 | 1.00 |
| 91_G | 181_V | 2.676 | 1.00 |
| 159_V | 253_F | 2.663 | 1.00 |
| 319_V | 334_I | 2.616 | 1.00 |
| 129_S | 136_W | 2.460 | 1.00 |
| 291_I | 334_I | 2.278 | 1.00 |
| 291_I | 317_A | 2.273 | 1.00 |
| 132_L | 136_W | 2.212 | 1.00 |
| 261_A | 265_T | 2.187 | 1.00 |
| 290_L | 342_F | 2.114 | 1.00 |
| 139_A | 275_M | 2.098 | 1.00 |
| 89_V | 104_A | 2.096 | 1.00 |
| 328_Q | 350_Q | 2.083 | 1.00 |
| 200_C | 215_R | 2.029 | 1.00 |
| 92_G | 101_V | 1.898 | 1.00 |
| 237_I | 247_A | 1.851 | 1.00 |
| 301_H | 352_E | 1.791 | 1.00 |
| 148_I | 267_L | 1.787 | 1.00 |
| 272_I | 277_G | 1.757 | 1.00 |
| 129_S | 132_L | 1.735 | 1.00 |
| 289_E | 293_A | 1.735 | 1.00 |
| 180_I | 184_C | 1.690 | 1.00 |
| 160_Y | 164_E | 1.682 | 1.00 |
| 286_G | 341_S | 1.674 | 1.00 |
| 285_S | 289_E | 1.666 | 1.00 |
| 158_L | 252_T | 1.653 | 1.00 |
| 257_I | 261_A | 1.646 | 1.00 |
| 298_L | 322_A | 1.636 | 1.00 |
| 290_L | 341_S | 1.598 | 1.00 |
| 134_F | 316_S | 1.597 | 1.00 |
| 253_F | 257_I | 1.573 | 1.00 |
| 75_C | 79_A | 1.570 | 1.00 |
| 288_K | 292_L | 1.564 | 1.00 |
| 303_L | 317_A | 1.548 | 1.00 |
| 215_R | 269_D | 1.539 | 1.00 |
| 88_E | 107_L | 1.536 | 1.00 |
| 279_P | 305_I | 1.536 | 1.00 |
| 304_H | 346_S | 1.534 | 1.00 |
| 152_W | 263_T | 1.527 | 1.00 |
| 183_S | 232_L | 1.514 | 1.00 |
| 318_H | 352_E | 1.509 | 1.00 |
| 294_V | 337_A | 1.502 | 1.00 |
| 117_S | 216_A | 1.490 | 1.00 |
| 166_L | 253_F | 1.489 | 1.00 |
| 276_E | 314_I | 1.488 | 1.00 |
| 106_H | 220_H | 1.483 | 1.00 |
| 176_T | 239_Y | 1.475 | 0.99 |
| 229_I | 233_I | 1.468 | 0.99 |
| 103_D | 228_S | 1.434 | 0.99 |
| 115_L | 119_F | 1.432 | 0.99 |
| 71_K | 125_S | 1.413 | 0.99 |
| 190_I | 222_L | 1.400 | 0.99 |
| 268_K | 272_I | 1.394 | 0.99 |
| 336_K | 340_K | 1.393 | 0.99 |
| 106_H | 110_D | 1.372 | 0.99 |
| 301_H | 320_A | 1.367 | 0.99 |
| 159_V | 256_S | 1.363 | 0.99 |
| 189_N | 221_A | 1.344 | 0.99 |
| 294_V | 330_V | 1.338 | 0.99 |
| 76_S | 195_V | 1.329 | 0.99 |
| 331_R | 350_Q | 1.314 | 0.99 |
| 95_A | 178_M | 1.310 | 0.98 |
| 71_K | 122_W | 1.289 | 0.98 |
| 162_A | 252_T | 1.288 | 0.98 |
| 202_G | 205_H | 1.286 | 0.98 |
| 103_D | 106_H | 1.283 | 0.98 |
| 329_V | 333_E | 1.282 | 0.98 |
| 131_R | 136_W | 1.276 | 0.98 |
| 163_C | 253_F | 1.270 | 0.98 |
| 299_S | 320_A | 1.258 | 0.98 |
| 142_L | 275_M | 1.241 | 0.98 |
| 193_T | 221_A | 1.241 | 0.98 |
| 217_A | 220_H | 1.240 | 0.98 |
| 166_L | 246_I | 1.239 | 0.98 |
| 133_T | 278_V | 1.231 | 0.98 |
| 291_I | 300_V | 1.225 | 0.97 |
| 334_I | 349_I | 1.218 | 0.97 |
| 247_A | 251_C | 1.208 | 0.97 |
| 290_L | 337_A | 1.193 | 0.97 |
| 141_I | 273_L | 1.189 | 0.97 |
| 198_Q | 202_G | 1.186 | 0.97 |
| 296_G | 330_V | 1.185 | 0.97 |
| 98_L | 162_A | 1.183 | 0.97 |
| 331_R | 351_M | 1.181 | 0.97 |
| 121_L | 213_S | 1.166 | 0.96 |
| 184_C | 188_A | 1.162 | 0.96 |
| 194_V | 198_Q | 1.156 | 0.96 |
| 84_F | 192_L | 1.153 | 0.96 |
| 163_C | 167_L | 1.148 | 0.96 |
| 152_W | 156_G | 1.140 | 0.96 |
| 335_A | 339_S | 1.140 | 0.96 |
| 155_T | 260_L | 1.138 | 0.96 |
| 134_F | 306_W | 1.132 | 0.95 |
| 179_I | 232_L | 1.123 | 0.95 |
| 152_W | 264_I | 1.122 | 0.95 |
| 138_R | 276_E | 1.117 | 0.95 |
| 156_G | 260_L | 1.116 | 0.95 |
| 80_I | 188_A | 1.104 | 0.94 |
| 157_V | 160_Y | 1.098 | 0.94 |
| 197_H | 218_F | 1.097 | 0.94 |
| 294_V | 333_E | 1.093 | 0.94 |
| 294_V | 334_I | 1.093 | 0.94 |
| 304_H | 316_S | 1.093 | 0.94 |
| 162_A | 249_P | 1.093 | 0.94 |
| 284_Y | 305_I | 1.092 | 0.94 |
| 156_G | 160_Y | 1.090 | 0.94 |
| 234_S | 251_C | 1.089 | 0.94 |
| 302_S | 346_S | 1.082 | 0.94 |
| 230_S | 251_C | 1.082 | 0.94 |
| 231_V | 234_S | 1.079 | 0.94 |
| 180_I | 183_S | 1.078 | 0.94 |
| 250_I | 254_I | 1.078 | 0.94 |
| 328_Q | 352_E | 1.077 | 0.93 |
| 86_I | 90_V | 1.076 | 0.93 |
| 325_R | 329_V | 1.072 | 0.93 |
| 328_Q | 353_S | 1.072 | 0.93 |
| 90_V | 94_I | 1.072 | 0.93 |
| 172_Q | 175_A | 1.071 | 0.93 |
| 196_L | 221_A | 1.071 | 0.93 |
| 120_S | 143_G | 1.070 | 0.93 |
| 332_R | 335_A | 1.069 | 0.93 |
| 107_L | 227_Q | 1.063 | 0.93 |
| 92_G | 104_A | 1.061 | 0.93 |
| 78_S | 111_L | 1.058 | 0.93 |
| 223_G | 266_I | 1.058 | 0.93 |
| 287_V | 338_L | 1.058 | 0.93 |
| 82_F | 86_I | 1.057 | 0.93 |
| 161_L | 164_E | 1.056 | 0.93 |
| 315_L | 344_M | 1.050 | 0.92 |
| 82_F | 111_L | 1.046 | 0.92 |
| 286_G | 342_F | 1.041 | 0.92 |
| 323_A | 355_V | 1.040 | 0.92 |
| 182_S | 235_A | 1.039 | 0.92 |
| 246_I | 249_P | 1.039 | 0.92 |
| 276_E | 306_W | 1.038 | 0.92 |
| 72_W | 195_V | 1.036 | 0.92 |
| 264_I | 268_K | 1.036 | 0.92 |
| 279_P | 282_L | 1.036 | 0.92 |
| 220_H | 223_G | 1.035 | 0.92 |
| 327_S | 351_M | 1.034 | 0.92 |
| 124_S | 128_P | 1.031 | 0.91 |
| 315_L | 349_I | 1.022 | 0.91 |
| 75_C | 118_L | 1.022 | 0.91 |
| 286_G | 289_E | 1.015 | 0.91 |
| 81_C | 110_D | 1.015 | 0.91 |
| 81_C | 106_H | 1.014 | 0.90 |
| 290_L | 294_V | 1.011 | 0.90 |
| 84_F | 188_A | 1.005 | 0.90 |
| 179_I | 239_Y | 1.004 | 0.90 |
| 300_V | 317_A | 1.000 | 0.90 |
| 280_K | 283_N | 1.000 | 0.90 |
| 74_L | 117_S | 1.000 | 0.90 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3j1zP | 2 | 0.794 | 100 | 0.334 | Contact Map |
| 3h90A | 4 | 0.7588 | 100 | 0.351 | Contact Map |
| 3bypA | 2 | 0.2087 | 99.6 | 0.823 | Contact Map |
| 2zztA | 1 | 0.2087 | 99.6 | 0.827 | Contact Map |
| 3cq1A | 1 | 0.2439 | 44.6 | 0.962 | Contact Map |
| 3lnoA | 6 | 0.2575 | 39.2 | 0.963 | Contact Map |
| 2jsxA | 1 | 0.2547 | 30.6 | 0.965 | Contact Map |
| 4k0jA | 6 | 0.3333 | 23.9 | 0.967 | Contact Map |
| 1uwdA | 1 | 0.2466 | 18.4 | 0.968 | Contact Map |
| 1t4aA | 2 | 0.2168 | 16.7 | 0.969 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)