| P60492 RL21_THET8 |
| ID: | 1379205224 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 101 (100) | ||
| Sequences: | 1522 (968.1) | ||
| Seq/Len: | 15.220 | ||
| Nf(neff/√len): | 96.8 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 15.220).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 60_E | 97_K | 3.882 | 1.00 |
| 28_E | 31_A | 3.453 | 1.00 |
| 16_P | 98_E | 3.361 | 1.00 |
| 6_K | 37_V | 3.292 | 1.00 |
| 66_R | 90_P | 3.249 | 1.00 |
| 27_A | 61_V | 3.115 | 1.00 |
| 71_L | 84_K | 2.946 | 1.00 |
| 15_E | 18_L | 2.715 | 1.00 |
| 34_E | 56_S | 2.613 | 1.00 |
| 59_A | 96_I | 2.517 | 1.00 |
| 60_E | 95_L | 2.473 | 1.00 |
| 21_R | 93_E | 2.199 | 1.00 |
| 32_T | 58_V | 2.122 | 1.00 |
| 76_K | 81_Y | 2.087 | 1.00 |
| 56_S | 100_R | 1.981 | 1.00 |
| 40_L | 45_T | 1.874 | 1.00 |
| 22_V | 94_L | 1.873 | 1.00 |
| 35_L | 94_L | 1.873 | 1.00 |
| 19_K | 93_E | 1.788 | 1.00 |
| 75_F | 78_K | 1.784 | 1.00 |
| 7_T | 22_V | 1.681 | 1.00 |
| 59_A | 94_L | 1.635 | 1.00 |
| 34_E | 58_V | 1.551 | 1.00 |
| 6_K | 51_V | 1.514 | 1.00 |
| 10_K | 23_E | 1.506 | 1.00 |
| 63_G | 93_E | 1.473 | 1.00 |
| 62_L | 95_L | 1.430 | 1.00 |
| 19_K | 95_L | 1.411 | 1.00 |
| 65_G | 91_Y | 1.411 | 1.00 |
| 5_V | 35_L | 1.339 | 0.99 |
| 40_L | 47_V | 1.337 | 0.99 |
| 74_K | 81_Y | 1.334 | 0.99 |
| 21_R | 67_G | 1.315 | 0.99 |
| 7_T | 23_E | 1.302 | 0.99 |
| 35_L | 59_A | 1.264 | 0.99 |
| 72_V | 85_K | 1.220 | 0.98 |
| 32_T | 97_K | 1.206 | 0.98 |
| 27_A | 31_A | 1.198 | 0.98 |
| 73_S | 84_K | 1.141 | 0.97 |
| 58_V | 98_E | 1.140 | 0.97 |
| 47_V | 50_P | 1.135 | 0.97 |
| 52_V | 55_A | 1.126 | 0.97 |
| 50_P | 54_G | 1.107 | 0.96 |
| 73_S | 82_R | 1.099 | 0.96 |
| 29_P | 63_G | 1.079 | 0.95 |
| 37_V | 51_V | 1.070 | 0.95 |
| 19_K | 62_L | 1.054 | 0.95 |
| 41_G | 44_K | 1.053 | 0.95 |
| 7_T | 12_Y | 1.025 | 0.93 |
| 61_V | 92_T | 1.016 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3bboT | 1 | 1 | 100 | 0.029 | Contact Map |
| 3v2dV | 1 | 1 | 100 | 0.029 | Contact Map |
| 4kixR | 1 | 1 | 100 | 0.04 | Contact Map |
| 3j3wR | 1 | 1 | 100 | 0.043 | Contact Map |
| 4ce4V | 1 | 0.9901 | 100 | 0.05 | Contact Map |
| 2zjrO | 1 | 0.9307 | 100 | 0.07 | Contact Map |
| 2y4xA | 2 | 0.5149 | 42.9 | 0.9 | Contact Map |
| 2lc4A | 1 | 0.6337 | 27.1 | 0.91 | Contact Map |
| 4av2M | 12 | 0.5347 | 26.1 | 0.911 | Contact Map |
| 2ekiA | 1 | 0.3069 | 14.5 | 0.921 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)