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OPENSEQ.org

human EIF2A 1-420

ID: 1377320903 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 50% gaps before running GREMLIN.
Length: 420 (412)
Sequences: 413 (299.1)
Seq/Len: 1.002
Nf(neff/√len): 14.7

HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.002).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 50% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
281_W413_W2.3500.99
126_K147_N2.2250.98
374_T390_W2.1810.98
330_V372_I2.0950.97
92_T112_Y2.0890.97
45_S83_F1.9500.95
272_K290_A1.8970.94
9_T44_F1.8330.92
59_V92_T1.7960.91
285_S415_P1.6990.87
10_V17_Y1.6760.86
289_C312_F1.6530.85
184_V372_I1.6280.84
75_L94_Q1.6150.84
283_S326_G1.5650.81
195_P288_F1.5430.80
126_K144_R1.5080.77
231_K247_G1.5020.77
127_K387_Y1.4950.76
216_K288_F1.4840.76
373_L411_V1.4800.75
48_G117_G1.4790.75
61_I111_L1.4770.75
73_F157_N1.4540.74
320_A329_L1.4530.74
109_L246_T1.4530.74
334_F339_G1.4500.73
83_F283_S1.4450.73
63_S67_K1.4370.72
330_V341_M1.4320.72
228_W281_W1.4040.70
279_V288_F1.3990.69
99_S103_T1.3980.69
211_A235_V1.3910.69
52_A83_F1.3870.68
90_L343_V1.3830.68
50_L226_M1.3760.68
220_K253_E1.3730.67
190_G196_S1.3610.66
150_V170_I1.3410.65
319_A332_A1.3340.64
90_L114_V1.3280.64
31_F122_S1.3250.63
52_A322_Y1.3230.63
81_L390_W1.2990.61
16_L279_V1.2880.60
135_W289_C1.2830.60
18_M59_V1.2820.60
198_V237_V1.2770.59
321_Y387_Y1.2760.59
331_L344_W1.2760.59
228_W302_F1.2750.59
87_N367_P1.2670.58
185_A235_V1.2660.58
289_C390_W1.2630.58
289_C384_N1.2610.58
358_S384_N1.2610.58
119_C323_S1.2480.56
313_G331_L1.2440.56
19_V61_I1.2440.56
57_E81_L1.2400.56
252_G275_P1.2380.56
296_P345_D1.2380.56
10_V14_E1.2370.55
145_N364_A1.2360.55
112_Y343_V1.2360.55
327_H370_E1.2300.55
358_S389_I1.2280.55
46_K120_L1.2220.54
276_I327_H1.2180.54
358_S369_G1.2110.53
50_L89_V1.2090.53
82_E329_L1.2090.53
47_D326_G1.2010.52
289_C329_L1.1920.51
331_L343_V1.1860.51
60_N92_T1.1780.50
189_P300_T1.1770.50
323_S332_A1.1770.50
4_S416_F1.1760.50
76_L90_L1.1750.50
139_E178_G1.1750.50
98_T101_D1.1720.49
94_Q106_I1.1720.49
202_Q264_E1.1700.49
281_W365_W1.1690.49
165_L204_P1.1690.49
360_S374_T1.1670.49
82_E397_L1.1650.49
346_V349_Y1.1650.49
60_N70_L1.1640.49
109_L144_R1.1630.49
16_L87_N1.1590.48
198_V397_L1.1560.48
376_T386_G1.1540.48
51_F92_T1.1450.47
288_F365_W1.1410.47
19_V114_V1.1410.47
314_T341_M1.1380.46
300_T308_P1.1280.45
378_A382_R1.1260.45
119_C373_L1.1260.45
200_L389_I1.1240.45
247_G360_S1.1230.45
143_A184_V1.1210.45
118_T343_V1.1200.45
152_F177_P1.1200.45
59_V355_P1.1180.44
305_K352_I1.1160.44
6_P33_R1.1160.44
256_L303_N1.1120.44
175_L301_I1.1110.44
360_S369_G1.1100.44
46_K87_N1.1080.44
270_L365_W1.1060.43
90_L388_K1.1050.43
69_L282_N1.1020.43
375_A408_L1.1010.43
273_N387_Y1.1010.43
166_H191_S1.1010.43
138_D141_L1.1000.43
9_T20_N1.0990.43
92_T142_C1.0980.43
247_G256_L1.0980.43
195_P216_K1.0950.42
187_Y190_G1.0930.42
170_I346_V1.0890.42
8_L369_G1.0890.42
254_Q279_V1.0860.42
280_V390_W1.0850.41
52_A90_L1.0840.41
125_Q375_A1.0820.41
228_W318_N1.0820.41
111_L114_V1.0790.41
45_S84_S1.0760.41
62_I140_T1.0760.41
133_P279_V1.0740.40
115_K200_L1.0730.40
278_D335_G1.0730.40
301_I330_V1.0720.40
39_C98_T1.0710.40
274_G277_Y1.0710.40
356_V390_W1.0700.40
44_F260_A1.0700.40
146_V165_L1.0690.40
187_Y254_Q1.0690.40
26_T41_V1.0680.40
87_N366_C1.0660.40
304_L372_I1.0620.39
96_Y100_K1.0620.39
379_P382_R1.0610.39
75_L78_A1.0600.39
226_M284_S1.0590.39
51_F329_L1.0560.39
50_L141_L1.0560.39
147_N153_F1.0540.39
44_F178_G1.0530.39
102_G105_G1.0530.39
47_D282_N1.0520.39
143_A185_A1.0510.38
4_S417_L1.0500.38
323_S327_H1.0480.38
131_W228_W1.0470.38
333_G339_G1.0470.38
205_N307_D1.0470.38
126_K153_F1.0450.38
84_S369_G1.0430.38
99_S102_G1.0430.38
276_I355_P1.0430.38
63_S265_S1.0430.38
110_Q119_C1.0430.38
245_K345_D1.0420.38
42_C319_A1.0400.37
314_T389_I1.0390.37
113_D167_L1.0380.37
86_K167_L1.0370.37
70_L120_L1.0370.37
235_V350_K1.0360.37
193_G388_K1.0360.37
196_S296_P1.0340.37
73_F90_L1.0340.37
276_I330_V1.0340.37
176_S228_W1.0340.37
19_V410_Q1.0310.37
46_K200_L1.0300.37
259_I302_F1.0290.37
341_M388_K1.0290.37
290_A296_P1.0280.36
272_K376_T1.0270.36
333_G336_N1.0270.36
45_S326_G1.0260.36
217_S375_A1.0250.36
294_F305_K1.0250.36
159_N233_T1.0250.36
96_Y99_S1.0250.36
162_A372_I1.0240.36
330_V355_P1.0240.36
70_L91_A1.0240.36
276_I292_Y1.0240.36
131_W256_L1.0230.36
60_N69_L1.0230.36
193_G216_K1.0220.36
309_V370_E1.0210.36
231_K304_L1.0200.36
228_W413_W1.0190.36
123_F393_T1.0180.36
18_M212_A1.0180.36
230_K387_Y1.0180.36
294_F381_L1.0170.35
115_K152_F1.0170.35
201_Y235_V1.0140.35
90_L136_S1.0140.35
170_I174_V1.0140.35
139_E329_L1.0140.35
52_A390_W1.0130.35
24_H414_Q1.0120.35
336_N379_P1.0120.35
8_L63_S1.0110.35
109_L134_S1.0110.35
218_F318_N1.0090.35
128_M284_S1.0080.35
111_L276_I1.0060.35
320_A394_G1.0060.35
65_T120_L1.0050.34
299_A331_L1.0020.34
24_H417_L1.0000.34
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (08Feb14)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3sfzA10.90241000.389Contact Map
1nr0A10.89761000.407Contact Map
2ymuA20.87621000.462Contact Map
2oajA10.89051000.479Contact Map
1pguA20.88811000.484Contact Map
3mkqA30.86191000.486Contact Map
1vyhC100.71431000.5Contact Map
2j04B20.77381000.504Contact Map
4bzjA20.82621000.51Contact Map
3jroA10.75711000.513Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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