| human EIF2A 1-420 |
| ID: | 1377319292 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 420 (394) | ||
| Sequences: | 7273 (5706.4) | ||
| Seq/Len: | 18.459 | ||
| Nf(neff/√len): | 287.5 |
HHblits Results: (2013_03)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 18.459).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 59_V | 92_T | 3.175 | 1.00 |
| 75_L | 92_T | 2.865 | 1.00 |
| 272_K | 290_A | 2.712 | 1.00 |
| 53_W | 62_I | 2.711 | 1.00 |
| 52_A | 83_F | 2.708 | 1.00 |
| 92_T | 112_Y | 2.509 | 1.00 |
| 319_A | 332_A | 2.472 | 1.00 |
| 126_K | 144_R | 2.456 | 1.00 |
| 52_A | 90_L | 2.376 | 1.00 |
| 41_V | 54_G | 2.373 | 1.00 |
| 341_M | 374_T | 2.366 | 1.00 |
| 362_Y | 375_A | 2.314 | 1.00 |
| 331_L | 344_W | 2.298 | 1.00 |
| 290_A | 302_F | 2.282 | 1.00 |
| 144_R | 153_F | 2.248 | 1.00 |
| 373_L | 413_W | 2.240 | 1.00 |
| 35_S | 53_W | 2.223 | 1.00 |
| 132_C | 145_N | 2.158 | 1.00 |
| 374_T | 390_W | 2.157 | 1.00 |
| 313_G | 331_L | 2.149 | 1.00 |
| 80_C | 93_W | 2.138 | 1.00 |
| 376_T | 384_N | 2.110 | 1.00 |
| 330_V | 372_I | 2.068 | 1.00 |
| 330_V | 365_W | 2.065 | 1.00 |
| 358_S | 374_T | 2.039 | 1.00 |
| 299_A | 331_L | 1.983 | 1.00 |
| 75_L | 78_A | 1.979 | 1.00 |
| 289_C | 322_Y | 1.972 | 1.00 |
| 289_C | 329_L | 1.949 | 1.00 |
| 52_A | 81_L | 1.943 | 1.00 |
| 109_L | 144_R | 1.937 | 1.00 |
| 143_A | 175_L | 1.932 | 1.00 |
| 113_D | 116_T | 1.930 | 1.00 |
| 91_A | 135_W | 1.926 | 1.00 |
| 324_P | 369_G | 1.914 | 1.00 |
| 219_F | 237_V | 1.910 | 1.00 |
| 278_D | 291_V | 1.898 | 1.00 |
| 110_Q | 119_C | 1.893 | 1.00 |
| 16_L | 53_W | 1.868 | 1.00 |
| 94_Q | 106_I | 1.846 | 1.00 |
| 60_N | 72_S | 1.822 | 1.00 |
| 110_Q | 122_S | 1.821 | 1.00 |
| 46_K | 87_N | 1.806 | 1.00 |
| 126_K | 147_N | 1.803 | 1.00 |
| 219_F | 223_K | 1.794 | 1.00 |
| 91_A | 142_C | 1.777 | 1.00 |
| 321_Y | 364_A | 1.766 | 1.00 |
| 63_S | 66_N | 1.749 | 1.00 |
| 172_D | 192_K | 1.744 | 1.00 |
| 272_K | 296_P | 1.742 | 1.00 |
| 60_N | 69_L | 1.729 | 1.00 |
| 225_T | 238_I | 1.727 | 1.00 |
| 150_V | 191_S | 1.723 | 1.00 |
| 191_S | 201_Y | 1.722 | 1.00 |
| 185_A | 228_W | 1.716 | 1.00 |
| 91_A | 111_L | 1.714 | 1.00 |
| 198_V | 237_V | 1.707 | 1.00 |
| 388_K | 399_K | 1.699 | 1.00 |
| 185_A | 200_L | 1.672 | 1.00 |
| 345_D | 348_N | 1.663 | 1.00 |
| 10_V | 19_V | 1.649 | 1.00 |
| 373_L | 411_V | 1.629 | 1.00 |
| 333_G | 338_R | 1.618 | 1.00 |
| 254_Q | 290_A | 1.602 | 1.00 |
| 342_E | 354_K | 1.597 | 1.00 |
| 280_V | 321_Y | 1.589 | 1.00 |
| 45_S | 83_F | 1.586 | 1.00 |
| 323_S | 365_W | 1.574 | 1.00 |
| 404_S | 408_L | 1.573 | 1.00 |
| 330_V | 343_V | 1.565 | 1.00 |
| 52_A | 61_I | 1.560 | 1.00 |
| 9_T | 44_F | 1.556 | 1.00 |
| 300_T | 311_D | 1.551 | 1.00 |
| 43_I | 82_E | 1.524 | 1.00 |
| 282_N | 322_Y | 1.506 | 1.00 |
| 236_L | 281_W | 1.497 | 1.00 |
| 289_C | 301_I | 1.479 | 1.00 |
| 283_S | 326_G | 1.462 | 1.00 |
| 237_V | 257_H | 1.455 | 1.00 |
| 272_K | 276_I | 1.449 | 1.00 |
| 143_A | 184_V | 1.428 | 1.00 |
| 338_R | 342_E | 1.428 | 1.00 |
| 330_V | 363_F | 1.420 | 1.00 |
| 272_K | 292_Y | 1.416 | 1.00 |
| 92_T | 110_Q | 1.412 | 1.00 |
| 236_L | 288_F | 1.397 | 0.99 |
| 376_T | 386_G | 1.390 | 0.99 |
| 143_A | 152_F | 1.375 | 0.99 |
| 85_P | 139_E | 1.362 | 0.99 |
| 82_E | 134_S | 1.362 | 0.99 |
| 91_A | 133_P | 1.361 | 0.99 |
| 94_Q | 108_N | 1.360 | 0.99 |
| 177_P | 232_A | 1.359 | 0.99 |
| 69_L | 119_C | 1.353 | 0.99 |
| 363_F | 374_T | 1.347 | 0.99 |
| 244_D | 251_Y | 1.338 | 0.99 |
| 35_S | 39_C | 1.336 | 0.99 |
| 20_N | 23_P | 1.333 | 0.99 |
| 119_C | 122_S | 1.330 | 0.99 |
| 289_C | 320_A | 1.323 | 0.99 |
| 236_L | 256_L | 1.322 | 0.99 |
| 313_G | 333_G | 1.322 | 0.99 |
| 313_G | 317_R | 1.303 | 0.99 |
| 166_H | 195_P | 1.303 | 0.99 |
| 283_S | 322_Y | 1.302 | 0.99 |
| 50_L | 61_I | 1.301 | 0.99 |
| 53_W | 60_N | 1.299 | 0.99 |
| 279_V | 290_A | 1.288 | 0.99 |
| 301_I | 322_Y | 1.279 | 0.99 |
| 89_V | 111_L | 1.272 | 0.99 |
| 300_T | 308_P | 1.272 | 0.99 |
| 384_N | 388_K | 1.271 | 0.99 |
| 193_G | 197_F | 1.266 | 0.99 |
| 358_S | 384_N | 1.262 | 0.99 |
| 343_V | 365_W | 1.259 | 0.99 |
| 330_V | 341_M | 1.255 | 0.99 |
| 272_K | 302_F | 1.254 | 0.99 |
| 42_C | 53_W | 1.252 | 0.99 |
| 310_F | 398_H | 1.249 | 0.99 |
| 75_L | 106_I | 1.243 | 0.98 |
| 366_C | 413_W | 1.242 | 0.98 |
| 357_A | 374_T | 1.241 | 0.98 |
| 258_Y | 261_T | 1.235 | 0.98 |
| 51_F | 64_V | 1.226 | 0.98 |
| 134_S | 174_V | 1.226 | 0.98 |
| 230_K | 285_S | 1.225 | 0.98 |
| 75_L | 94_Q | 1.220 | 0.98 |
| 137_E | 179_P | 1.215 | 0.98 |
| 333_G | 340_Q | 1.213 | 0.98 |
| 342_E | 351_L | 1.202 | 0.98 |
| 126_K | 130_N | 1.202 | 0.98 |
| 374_T | 384_N | 1.202 | 0.98 |
| 374_T | 388_K | 1.200 | 0.98 |
| 43_I | 81_L | 1.198 | 0.98 |
| 46_K | 83_F | 1.196 | 0.98 |
| 202_Q | 205_N | 1.179 | 0.98 |
| 143_A | 173_F | 1.173 | 0.98 |
| 185_A | 235_V | 1.172 | 0.98 |
| 84_S | 87_N | 1.172 | 0.98 |
| 291_V | 317_R | 1.171 | 0.98 |
| 373_L | 389_I | 1.165 | 0.97 |
| 323_S | 327_H | 1.164 | 0.97 |
| 328_I | 343_V | 1.162 | 0.97 |
| 136_S | 175_L | 1.149 | 0.97 |
| 81_L | 92_T | 1.149 | 0.97 |
| 134_S | 173_F | 1.146 | 0.97 |
| 328_I | 371_H | 1.145 | 0.97 |
| 287_E | 328_I | 1.140 | 0.97 |
| 176_S | 228_W | 1.137 | 0.97 |
| 50_L | 89_V | 1.127 | 0.97 |
| 282_N | 286_T | 1.126 | 0.97 |
| 364_A | 412_S | 1.125 | 0.97 |
| 228_W | 365_W | 1.116 | 0.96 |
| 317_R | 331_L | 1.113 | 0.96 |
| 137_E | 175_L | 1.110 | 0.96 |
| 313_G | 338_R | 1.106 | 0.96 |
| 296_P | 300_T | 1.103 | 0.96 |
| 84_S | 135_W | 1.102 | 0.96 |
| 280_V | 320_A | 1.091 | 0.96 |
| 366_C | 370_E | 1.091 | 0.96 |
| 321_Y | 363_F | 1.089 | 0.96 |
| 10_V | 17_Y | 1.087 | 0.96 |
| 52_A | 59_V | 1.086 | 0.96 |
| 185_A | 198_V | 1.082 | 0.96 |
| 144_R | 151_H | 1.079 | 0.95 |
| 199_R | 215_N | 1.069 | 0.95 |
| 154_E | 157_N | 1.069 | 0.95 |
| 55_N | 58_K | 1.066 | 0.95 |
| 69_L | 396_I | 1.066 | 0.95 |
| 55_N | 60_N | 1.060 | 0.95 |
| 81_L | 90_L | 1.057 | 0.95 |
| 281_W | 288_F | 1.056 | 0.95 |
| 233_T | 286_T | 1.045 | 0.94 |
| 155_N | 346_V | 1.042 | 0.94 |
| 360_S | 374_T | 1.041 | 0.94 |
| 331_L | 342_E | 1.039 | 0.94 |
| 141_L | 183_K | 1.030 | 0.94 |
| 341_M | 363_F | 1.030 | 0.94 |
| 366_C | 373_L | 1.027 | 0.94 |
| 54_G | 78_A | 1.024 | 0.93 |
| 327_H | 370_E | 1.022 | 0.93 |
| 292_Y | 298_K | 1.019 | 0.93 |
| 281_W | 304_L | 1.018 | 0.93 |
| 324_P | 365_W | 1.015 | 0.93 |
| 68_G | 307_D | 1.015 | 0.93 |
| 226_M | 235_V | 1.014 | 0.93 |
| 89_V | 141_L | 1.012 | 0.93 |
| 366_C | 369_G | 1.009 | 0.93 |
| 277_Y | 318_N | 1.008 | 0.93 |
| 54_G | 81_L | 1.006 | 0.93 |
| 195_P | 199_R | 1.005 | 0.93 |
| 332_A | 374_T | 1.002 | 0.92 |
| 281_W | 322_Y | 1.002 | 0.92 |
| 328_I | 365_W | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3sfzA | 1 | 0.8881 | 100 | 0.236 | Contact Map |
| 1nr0A | 1 | 0.8952 | 100 | 0.263 | Contact Map |
| 2ymuA | 2 | 0.8762 | 100 | 0.324 | Contact Map |
| 1pguA | 2 | 0.8738 | 100 | 0.343 | Contact Map |
| 2oajA | 1 | 0.8929 | 100 | 0.343 | Contact Map |
| 3mkqA | 3 | 0.8619 | 100 | 0.35 | Contact Map |
| 2j04B | 2 | 0.7762 | 100 | 0.383 | Contact Map |
| 1vyhC | 10 | 0.6833 | 100 | 0.39 | Contact Map |
| 3jroA | 1 | 0.7595 | 100 | 0.396 | Contact Map |
| 4bzjA | 2 | 0.8738 | 100 | 0.399 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)