OPENSEQ.org - YAFP - ECOLI GREMLIN

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YAFP - Uncharacterized N-acetyltransferase YafP

UniProt:	Q47158	Sequence MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQISAWAQIDESRWKEKLAKSQVWVAIINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIKSESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG13153
Length:	150 (145)
Sequences:	10257
Seq/Len:	70.74

YAFP
Paralog alert: 0.87 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: ATDA PHNO RIMI RIMJ RIML YAFP YEDL YHHY YIAC YJAB YJGM YNCA YPEA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
7_R	62_I	3.821	1.00
7_R	13_D	2.907	1.00
23_R	84_D	2.88	1.00
94_S	122_Y	2.871	1.00
101_I	124_F	2.544	1.00
71_I	100_L	2.471	1.00
94_S	121_R	2.406	1.00
59_W	99_P	2.353	1.00
61_A	92_V	2.322	1.00
99_P	102_K	2.312	1.00
68_V	87_Y	2.259	1.00
116_K	120_E	2.222	1.00
9_Y	17_L	2.22	1.00
101_I	146_Y	2.198	1.00
60_V	67_P	2.027	1.00
120_E	126_T	2.021	1.00
98_K	122_Y	1.943	1.00
126_T	129_Q	1.896	1.00
63_I	68_V	1.855	1.00
117_P	120_E	1.784	1.00
97_L	122_Y	1.737	1.00
16_Q	19_A	1.719	1.00
87_Y	90_R	1.713	1.00
63_I	87_Y	1.689	1.00
69_G	83_V	1.663	1.00
62_I	65_A	1.577	1.00
94_S	98_K	1.562	1.00
127_V	145_R	1.515	1.00
73_R	100_L	1.51	1.00
15_Q	46_E	1.486	1.00
113_I	117_P	1.479	1.00
23_R	85_P	1.445	1.00
126_T	142_F	1.417	1.00
63_I	90_R	1.412	1.00
101_I	105_S	1.409	1.00
5_Q	62_I	1.392	0.99
66_Q	86_E	1.388	0.99
19_A	23_R	1.368	0.99
66_Q	84_D	1.36	0.99
16_Q	67_P	1.346	0.99
116_K	126_T	1.327	0.99
11_P	50_K	1.292	0.99
83_V	92_V	1.279	0.99
88_T	114_T	1.276	0.99
16_Q	20_I	1.269	0.99
46_E	50_K	1.252	0.99
58_V	72_S	1.241	0.98
110_D	129_Q	1.237	0.98
16_Q	66_Q	1.228	0.98
129_Q	140_T	1.224	0.98
110_D	140_T	1.18	0.98
99_P	103_S	1.173	0.98
71_I	96_L	1.165	0.97
97_L	124_F	1.163	0.97
84_D	87_Y	1.16	0.97
115_A	119_F	1.159	0.97
19_A	84_D	1.158	0.97
6_I	59_W	1.157	0.97
9_Y	60_V	1.154	0.97
68_V	92_V	1.133	0.97
62_I	67_P	1.118	0.96
110_D	142_F	1.104	0.96
131_R	140_T	1.092	0.96
18_C	22_I	1.09	0.96
109_V	112_S	1.088	0.96
116_K	144_M	1.085	0.96
89_R	114_T	1.079	0.95
7_R	60_V	1.078	0.95
91_G	94_S	1.064	0.95
113_I	116_K	1.061	0.95
21_F	70_F	1.054	0.95
20_I	24_A	1.054	0.95
16_Q	84_D	1.035	0.94
95_A	99_P	1.034	0.94
15_Q	19_A	1.02	0.93
95_A	98_K	1.018	0.93
70_F	82_F	1.016	0.93

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2fiwA	1	1	99.8	0.259	Contact Map	0.681
4e2aA	2	0.9933	99.8	0.273	Contact Map	0.766
2j8mA	2	0.9667	99.8	0.273	Contact Map	0.738
3fncA	2	0.9867	99.8	0.274	Contact Map	0.733
3dr6A	3	0.9667	99.8	0.279	Contact Map	0.836
1tiqA	2	0.9933	99.8	0.29	Contact Map	0.743
1gheA	2	0.9667	99.8	0.297	Contact Map	0.672
3kkwA	1	0.96	99.8	0.303	Contact Map	0.642
4l8aA	1	0.96	99.8	0.306	Contact Map	0.647
3fixA	4	0.9333	99.8	0.307	Contact Map	0.777

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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