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OPENSEQ.org
PUUA - Gamma-glutamylputrescine synthetase PuuA
UniProt: P78061 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13908
Length: 472 (448)
Sequences: 2643
Seq/Len: 5.90
PUUA
Paralog alert: 0.38 [within 20: 0.08] - ratio of genomes with paralogs
Cluster includes: GLNA PUUA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
312_A 398_L 5.394 1.00
428_I 450_N 5.069 1.00
285_I 310_M 4.687 1.00
310_M 390_I 3.795 1.00
146_Q 395_D 3.598 1.00
123_T 141_N 3.529 1.00
207_D 262_H 3.344 1.00
425_G 450_N 3.221 1.00
145_Q 149_Q 3.209 1.00
29_R 33_R 3.2 1.00
392_H 396_N 3.174 1.00
309_K 397_E 3.081 1.00
150_R 395_D 3.02 1.00
285_I 386_I 2.974 1.00
139_V 441_F 2.971 1.00
163_Y 227_S 2.962 1.00
126_D 130_A 2.797 1.00
462_E 466_E 2.738 1.00
295_V 306_L 2.713 1.00
305_P 397_E 2.697 1.00
316_D 396_N 2.685 1.00
317_L 430_N 2.683 1.00
143_L 388_A 2.606 1.00
320_S 430_N 2.597 1.00
207_D 261_K 2.45 1.00
145_Q 148_R 2.444 1.00
239_D 243_E 2.435 1.00
164_L 204_V 2.384 1.00
30_Y 34_Y 2.348 1.00
434_R 439_E 2.298 1.00
439_E 443_H 2.28 1.00
308_K 399_P 2.233 1.00
38_Q 210_E 2.2 1.00
424_L 446_H 2.196 1.00
255_V 266_A 2.194 1.00
309_K 398_L 2.186 1.00
325_L 382_V 2.182 1.00
309_K 312_A 2.161 1.00
144_W 242_L 2.14 1.00
156_V 383_M 2.126 1.00
204_V 208_I 2.094 1.00
239_D 247_D 2.061 1.00
243_E 247_D 2.051 1.00
144_W 241_V 2.026 1.00
240_N 243_E 2.021 1.00
426_E 429_E 1.981 1.00
419_R 422_D 1.98 1.00
421_S 457_E 1.972 1.00
215_Q 254_L 1.954 1.00
323_A 427_F 1.954 1.00
165_L 273_Y 1.94 1.00
454_L 457_E 1.904 1.00
320_S 433_L 1.89 1.00
312_A 400_L 1.885 1.00
25_Q 29_R 1.858 1.00
305_P 399_P 1.852 1.00
37_T 109_P 1.851 1.00
377_A 382_V 1.841 1.00
317_L 392_H 1.841 1.00
418_I 422_D 1.838 1.00
251_L 255_V 1.831 1.00
305_P 309_K 1.828 1.00
324_L 385_A 1.824 1.00
244_A 247_D 1.755 1.00
319_P 426_E 1.732 1.00
306_L 394_L 1.717 1.00
201_F 231_F 1.715 1.00
59_S 213_Q 1.712 1.00
427_F 442_C 1.697 1.00
455_Q 467_W 1.694 1.00
317_L 389_G 1.662 1.00
106_S 121_L 1.662 1.00
432_H 436_Y 1.656 1.00
147_L 154_P 1.647 1.00
212_A 254_L 1.637 1.00
431_D 435_R 1.632 1.00
27_V 104_L 1.629 1.00
45_T 249_L 1.629 1.00
321_S 325_L 1.625 1.00
208_I 255_V 1.621 0.99
148_R 153_F 1.589 0.99
428_I 443_H 1.586 0.99
310_M 386_I 1.566 0.99
43_L 119_Q 1.559 0.99
245_C 383_M 1.556 0.99
316_D 398_L 1.554 0.99
34_Y 116_F 1.536 0.99
133_D 440_R 1.534 0.99
428_I 431_D 1.523 0.99
42_V 122_L 1.519 0.99
26_E 29_R 1.515 0.99
303_D 307_L 1.511 0.99
311_L 368_V 1.499 0.99
239_D 244_A 1.493 0.99
235_L 244_A 1.493 0.99
29_R 32_E 1.492 0.99
305_P 308_K 1.488 0.99
435_R 439_E 1.475 0.99
31_L 58_V 1.473 0.99
407_N 410_E 1.473 0.99
312_A 401_Q 1.47 0.99
166_D 264_M 1.458 0.99
141_N 242_L 1.452 0.99
421_S 425_G 1.452 0.99
221_G 234_N 1.444 0.99
51_F 121_L 1.44 0.99
144_W 240_N 1.439 0.99
143_L 384_A 1.428 0.98
431_D 439_E 1.427 0.98
274_E 460_I 1.423 0.98
152_L 295_V 1.416 0.98
218_P 236_Y 1.393 0.98
211_L 254_L 1.39 0.98
119_Q 250_A 1.39 0.98
211_L 258_M 1.39 0.98
285_I 390_I 1.386 0.98
105_G 243_E 1.381 0.98
428_I 439_E 1.379 0.98
101_V 125_V 1.376 0.98
422_D 425_G 1.368 0.98
227_S 277_A 1.359 0.98
283_I 386_I 1.352 0.98
31_L 61_L 1.348 0.98
315_I 356_L 1.334 0.97
319_P 322_M 1.331 0.97
94_G 407_N 1.329 0.97
434_R 442_C 1.327 0.97
160_L 248_A 1.325 0.97
155_V 237_H 1.318 0.97
324_L 427_F 1.315 0.97
323_A 424_L 1.313 0.97
384_A 437_L 1.309 0.97
165_L 267_T 1.306 0.97
297_S 302_E 1.301 0.97
332_Y 420_Q 1.3 0.97
449_K 452_E 1.284 0.96
388_A 436_Y 1.28 0.96
212_A 217_I 1.279 0.96
312_A 399_P 1.267 0.96
163_Y 279_S 1.267 0.96
147_L 391_L 1.251 0.96
147_L 150_R 1.246 0.96
306_L 309_K 1.245 0.95
430_N 433_L 1.238 0.95
322_M 372_V 1.238 0.95
143_L 146_Q 1.236 0.95
289_N 293_E 1.235 0.95
454_L 458_R 1.211 0.95
133_D 438_G 1.211 0.95
100_C 124_M 1.199 0.94
38_Q 213_Q 1.191 0.94
424_L 453_L 1.184 0.94
309_K 399_P 1.179 0.93
308_K 400_L 1.178 0.93
425_G 429_E 1.177 0.93
107_L 118_G 1.172 0.93
211_L 214_L 1.171 0.93
270_A 376_D 1.17 0.93
163_Y 277_A 1.163 0.93
456_F 463_T 1.162 0.93
356_L 368_V 1.158 0.93
370_Y 386_I 1.157 0.93
324_L 442_C 1.152 0.92
320_S 426_E 1.152 0.92
321_S 385_A 1.15 0.92
220_D 236_Y 1.149 0.92
164_L 259_A 1.148 0.92
269_M 330_N 1.141 0.92
139_V 384_A 1.136 0.92
311_L 356_L 1.135 0.92
428_I 446_H 1.134 0.92
309_K 394_L 1.133 0.92
37_T 118_G 1.131 0.91
103_V 123_T 1.128 0.91
108_T 119_Q 1.125 0.91
425_G 428_I 1.104 0.90
140_L 241_V 1.099 0.90
347_W 358_I 1.097 0.90
317_L 396_N 1.093 0.90
328_N 376_D 1.083 0.89
146_Q 388_A 1.08 0.89
164_L 266_A 1.08 0.89
207_D 211_L 1.079 0.89
333_R 464_E 1.078 0.89
225_E 232_E 1.078 0.89
427_F 433_L 1.07 0.88
225_E 282_H 1.068 0.88
164_L 201_F 1.068 0.88
314_M 370_Y 1.06 0.88
152_L 306_L 1.059 0.87
301_G 308_K 1.057 0.87
319_P 422_D 1.055 0.87
135_E 138_N 1.049 0.87
27_V 120_M 1.048 0.87
270_A 374_G 1.042 0.86
443_H 447_A 1.038 0.86
27_V 107_L 1.035 0.86
131_P 138_N 1.034 0.86
434_R 443_H 1.03 0.85
460_I 464_E 1.029 0.85
39_Y 210_E 1.024 0.85
156_V 310_M 1.021 0.85
217_I 250_A 1.016 0.84
248_A 281_M 1.012 0.84
226_A 277_A 1.012 0.84
446_H 450_N 1.012 0.84
159_E 225_E 1.002 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4lniA120.90891000.142Contact Map0.841
3qajA120.9111000.144Contact Map0.892
4hppA10.89191000.15Contact Map0.705
4acfA60.93861000.15Contact Map0.792
1f52A120.91951000.16Contact Map0.815
3ng0A10.92581000.161Contact Map0.718
2j9iA120.86861000.208Contact Map0.321
3o6xA60.95131000.372Contact Map0.425
3fkyA200.76061000.381Contact Map0.542
2ojwA50.76911000.388Contact Map0.6
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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