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OPENSEQ.org
RSMF - Ribosomal RNA small subunit methyltransferase F
UniProt: P76273 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14023
Length: 479 (464)
Sequences: 534
Seq/Len: 1.15
RSMF
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
132_T 162_I 3.829 1.00
125_A 193_L 3.005 1.00
132_T 147_A 2.747 1.00
258_C 308_L 2.53 1.00
241_T 307_R 2.514 1.00
191_I 236_L 2.486 0.99
15_Q 252_E 2.386 0.99
230_D 264_T 2.289 0.99
11_A 215_P 2.255 0.99
241_T 309_R 2.203 0.98
193_L 229_I 2.096 0.98
136_S 167_I 2.068 0.97
108_V 135_I 2.06 0.97
402_V 442_L 2.059 0.97
270_E 309_R 2.054 0.97
261_L 265_Y 2.048 0.97
15_Q 250_N 2.022 0.97
164_R 468_L 1.934 0.96
453_A 460_L 1.894 0.95
119_Q 190_A 1.856 0.94
212_N 217_S 1.838 0.94
226_R 260_W 1.788 0.93
443_V 450_I 1.78 0.92
270_E 311_T 1.773 0.92
262_K 271_F 1.764 0.92
92_H 149_E 1.751 0.91
41_I 97_F 1.717 0.90
233_F 268_A 1.71 0.90
149_E 209_A 1.67 0.89
41_I 76_I 1.659 0.88
175_F 179_V 1.638 0.87
87_G 149_E 1.635 0.87
10_D 14_T 1.624 0.87
120_R 237_R 1.602 0.86
110_A 274_L 1.585 0.85
147_A 192_L 1.579 0.85
43_V 53_F 1.569 0.84
32_A 35_R 1.564 0.84
104_S 303_F 1.564 0.84
87_G 468_L 1.563 0.84
145_I 439_D 1.558 0.84
209_A 221_I 1.557 0.84
243_V 307_R 1.549 0.83
193_L 228_L 1.527 0.82
423_E 428_R 1.525 0.82
149_E 160_A 1.524 0.82
251_Q 256_A 1.523 0.82
363_E 386_K 1.515 0.81
364_L 403_I 1.507 0.81
149_E 164_R 1.5 0.80
149_E 468_L 1.496 0.80
134_Q 137_A 1.492 0.80
92_H 209_A 1.491 0.80
214_S 218_N 1.485 0.79
107_P 134_Q 1.477 0.79
17_R 27_D 1.471 0.78
155_V 172_L 1.462 0.78
165_C 224_T 1.458 0.78
417_T 420_E 1.449 0.77
200_E 248_T 1.445 0.77
92_H 164_R 1.445 0.77
87_G 164_R 1.421 0.75
98_Y 161_N 1.413 0.74
233_F 261_L 1.407 0.74
155_V 381_S 1.399 0.73
41_I 99_I 1.392 0.73
443_V 460_L 1.388 0.73
26_F 30_L 1.378 0.72
293_V 298_Y 1.365 0.71
184_V 235_A 1.364 0.71
44_N 47_K 1.357 0.70
388_A 395_Y 1.347 0.69
69_W 73_G 1.341 0.69
108_V 134_Q 1.335 0.68
149_E 221_I 1.334 0.68
399_H 464_Y 1.33 0.68
167_I 170_V 1.324 0.67
162_I 167_I 1.306 0.66
20_M 24_L 1.305 0.66
273_P 289_G 1.303 0.66
290_F 304_F 1.296 0.65
255_E 464_Y 1.293 0.65
440_D 452_L 1.287 0.64
186_E 239_G 1.283 0.64
236_L 310_K 1.281 0.64
231_S 234_H 1.279 0.63
255_E 292_H 1.277 0.63
465_P 468_L 1.269 0.63
107_P 151_S 1.268 0.62
454_K 462_N 1.266 0.62
205_K 213_W 1.264 0.62
132_T 145_I 1.258 0.62
388_A 398_Q 1.254 0.61
222_A 226_R 1.254 0.61
149_E 214_S 1.247 0.61
221_I 466_R 1.243 0.60
414_F 457_G 1.242 0.60
287_E 290_F 1.235 0.59
229_I 261_L 1.233 0.59
40_S 102_A 1.23 0.59
101_E 295_P 1.225 0.59
226_R 257_V 1.217 0.58
399_H 468_L 1.216 0.58
338_G 393_K 1.215 0.58
114_D 135_I 1.214 0.58
13_L 26_F 1.213 0.57
189_D 237_R 1.212 0.57
92_H 468_L 1.211 0.57
440_D 463_S 1.21 0.57
399_H 465_P 1.21 0.57
92_H 160_A 1.202 0.56
124_V 193_L 1.2 0.56
117_A 142_E 1.195 0.56
181_G 235_A 1.189 0.55
132_T 216_E 1.187 0.55
12_F 213_W 1.187 0.55
48_I 53_F 1.184 0.55
40_S 303_F 1.184 0.55
35_R 299_D 1.182 0.54
135_I 192_L 1.182 0.54
38_R 192_L 1.18 0.54
107_P 149_E 1.177 0.54
65_T 299_D 1.176 0.54
430_V 460_L 1.175 0.54
363_E 389_E 1.173 0.54
180_F 184_V 1.17 0.53
359_Q 362_K 1.169 0.53
107_P 192_L 1.164 0.53
209_A 466_R 1.159 0.52
110_A 305_V 1.156 0.52
111_L 379_R 1.156 0.52
209_A 399_H 1.154 0.52
217_S 220_E 1.153 0.52
67_I 402_V 1.153 0.52
76_I 99_I 1.151 0.51
421_A 445_F 1.151 0.51
219_Q 252_E 1.142 0.51
86_L 249_L 1.141 0.51
7_Y 362_K 1.14 0.50
364_L 390_T 1.137 0.50
105_M 291_L 1.134 0.50
221_I 224_T 1.132 0.50
87_G 205_K 1.132 0.50
276_D 469_V 1.131 0.50
31_A 34_Q 1.13 0.49
271_F 308_L 1.129 0.49
160_A 164_R 1.129 0.49
40_S 104_S 1.126 0.49
181_G 228_L 1.125 0.49
149_E 466_R 1.125 0.49
146_L 173_T 1.122 0.49
298_Y 452_L 1.12 0.49
73_G 206_D 1.119 0.48
300_C 391_H 1.117 0.48
31_A 35_R 1.115 0.48
87_G 209_A 1.114 0.48
70_C 109_A 1.113 0.48
72_E 138_R 1.111 0.48
67_I 73_G 1.109 0.47
241_T 464_Y 1.108 0.47
227_E 237_R 1.108 0.47
387_L 400_E 1.107 0.47
144_A 187_M 1.098 0.46
423_E 426_R 1.094 0.46
15_Q 251_Q 1.092 0.46
130_S 397_W 1.089 0.46
18_E 27_D 1.089 0.46
29_F 366_L 1.088 0.45
134_Q 165_C 1.084 0.45
168_S 309_R 1.084 0.45
92_H 107_P 1.083 0.45
69_W 421_A 1.082 0.45
143_G 394_G 1.078 0.44
42_R 213_W 1.078 0.44
178_R 231_S 1.077 0.44
69_W 402_V 1.076 0.44
11_A 14_T 1.075 0.44
218_N 221_I 1.074 0.44
117_A 140_N 1.069 0.44
120_R 238_P 1.069 0.44
53_F 74_F 1.067 0.43
147_A 231_S 1.067 0.43
243_V 443_V 1.066 0.43
28_D 31_A 1.066 0.43
135_I 261_L 1.066 0.43
209_A 468_L 1.061 0.43
36_P 107_P 1.06 0.43
105_M 125_A 1.056 0.42
69_W 97_F 1.055 0.42
239_G 308_L 1.054 0.42
135_I 138_R 1.052 0.42
391_H 396_R 1.051 0.42
119_Q 237_R 1.051 0.42
139_M 145_I 1.05 0.42
280_G 387_L 1.049 0.42
42_R 133_T 1.049 0.42
175_F 378_V 1.049 0.42
201_G 301_E 1.046 0.41
153_S 416_L 1.043 0.41
191_I 424_W 1.041 0.41
108_V 138_R 1.04 0.41
284_A 469_V 1.039 0.41
106_L 293_V 1.038 0.41
432_P 457_G 1.037 0.41
454_K 461_K 1.037 0.41
136_S 195_A 1.036 0.41
36_P 63_T 1.034 0.40
45_T 74_F 1.032 0.40
93_L 160_A 1.031 0.40
75_W 299_D 1.029 0.40
96_L 254_N 1.028 0.40
277_L 469_V 1.027 0.40
115_G 138_R 1.024 0.39
66_P 74_F 1.023 0.39
261_L 268_A 1.023 0.39
202_V 301_E 1.018 0.39
203_V 300_C 1.018 0.39
91_E 446_Q 1.017 0.39
69_W 206_D 1.016 0.39
160_A 221_I 1.016 0.39
15_Q 19_A 1.016 0.39
130_S 203_V 1.015 0.39
73_G 105_M 1.014 0.39
105_M 402_V 1.013 0.38
101_E 204_R 1.011 0.38
160_A 468_L 1.01 0.38
102_A 303_F 1.009 0.38
290_F 306_A 1.006 0.38
139_M 428_R 1.006 0.38
326_N 342_Q 1.006 0.38
43_V 137_A 1.001 0.37
87_G 466_R 1.001 0.37
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2frxA411000.311Contact Map0.533
3m4xA10.92691000.371Contact Map0.546
3m6wA10.93111000.399Contact Map0.503
1ixkA10.62841000.622Contact Map0.588
2yxlA10.62211000.631Contact Map0.554
1sqgA10.60751000.648Contact Map0.526
2b9eA10.5951000.649Contact Map0.573
3ajdA10.56161000.678Contact Map0.62
4fzvA10.62841000.694Contact Map0.501
2vdvE20.478199.90.845Contact Map0.31
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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