OPENSEQ.org - MSRC - ECOLI GREMLIN

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MSRC - Free methionine-R-sulfoxide reductase

UniProt:	P76270	Sequence MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDINWAGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDAASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFFASVAG	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG14020
Length:	165 (151)
Sequences:	817
Seq/Len:	5.41

MSRC
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
80_V	118_I	2.939	1.00
90_D	93_V	2.923	1.00
85_V	141_R	2.867	1.00
89_E	130_G	2.836	1.00
23_S	51_E	2.807	1.00
137_E	141_R	2.792	1.00
71_V	80_V	2.726	1.00
108_I	140_L	2.657	1.00
92_H	105_N	2.647	1.00
50_L	71_V	2.481	1.00
3_K	133_T	2.381	1.00
87_R	137_E	2.329	1.00
36_Y	45_A	2.318	1.00
105_N	129_F	2.299	1.00
14_F	31_T	2.136	1.00
82_R	86_Q	2.131	1.00
41_D	128_V	2.087	1.00
28_L	49_L	2.086	1.00
72_G	81_A	2.074	1.00
56_V	68_R	2.003	1.00
85_V	110_L	1.976	1.00
83_N	113_V	1.96	1.00
85_V	137_E	1.846	1.00
122_L	144_V	1.779	1.00
50_L	80_V	1.726	1.00
35_L	122_L	1.722	1.00
72_G	78_T	1.71	1.00
138_Q	142_Q	1.62	0.99
31_T	143_L	1.564	0.99
30_N	60_F	1.516	0.99
28_L	147_L	1.493	0.99
26_A	56_V	1.458	0.98
110_L	141_R	1.454	0.98
134_D	138_Q	1.437	0.98
49_L	120_G	1.432	0.98
26_A	68_R	1.392	0.98
83_N	118_I	1.384	0.98
108_I	137_E	1.341	0.97
21_E	68_R	1.336	0.97
108_I	124_I	1.315	0.96
112_L	144_V	1.315	0.96
54_T	68_R	1.3	0.96
135_E	139_G	1.267	0.95
7_Y	139_G	1.26	0.95
42_I	127_T	1.233	0.94
112_L	120_G	1.208	0.93
134_D	137_E	1.203	0.93
97_H	104_S	1.19	0.93
50_L	53_D	1.162	0.92
14_F	18_M	1.155	0.91
70_P	73_R	1.149	0.91
110_L	144_V	1.132	0.90
11_N	146_Q	1.132	0.90
78_T	94_F	1.131	0.90
126_S	131_R	1.122	0.90
11_N	139_G	1.112	0.89
21_E	26_A	1.099	0.88
43_N	127_T	1.096	0.88
11_N	142_Q	1.087	0.88
63_K	103_A	1.084	0.87
42_I	124_I	1.077	0.87
133_T	136_D	1.074	0.87
87_R	108_I	1.066	0.86
78_T	82_R	1.06	0.86
81_A	117_Q	1.059	0.86
104_S	127_T	1.058	0.86
92_H	97_H	1.049	0.85
83_N	117_Q	1.032	0.84
50_L	118_I	1.025	0.83
15_N	149_K	1.024	0.83
9_D	12_R	1.014	0.82
113_V	118_I	1.01	0.82
63_K	101_D	1.007	0.82

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
1vhmA	2	1	99.9	0.676	Contact Map	0.868
3rfbA	2	0.9758	99.9	0.687	Contact Map	0.825
1f5mA	2	0.9333	99.9	0.691	Contact Map	0.88
3kshA	1	0.9212	99.9	0.694	Contact Map	0.793
3mmhA	2	0.9515	99.7	0.739	Contact Map	0.768
3e0yA	2	0.9879	99.4	0.788	Contact Map	0.57
4g3vA	2	0.9758	99.4	0.794	Contact Map	0.672
1ykdA	2	0.9879	99.3	0.8	Contact Map	0.456
1mc0A	1	0.9939	99.3	0.805	Contact Map	0.585
3dbaA	2	0.9515	99.2	0.809	Contact Map	0.644

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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