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OPENSEQ.org
SAD - Succinate semialdehyde dehydrogenase [NAD(P)+] Sad
UniProt: P76149 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13817
Length: 462 (454)
Sequences: 17858
Seq/Len: 39.33
SAD
Paralog alert: 0.85 [within 20: 0.24] - ratio of genomes with paralogs
Cluster includes: ABDH ALDA ALDB ASTD BETB EUTE FEAB GABD PROA PUUC SAD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
278_E 377_K 5.373 1.00
222_A 226_A 3.704 1.00
278_E 375_I 3.172 1.00
250_E 287_R 3.105 1.00
245_N 277_E 2.839 1.00
39_A 204_R 2.756 1.00
313_F 317_D 2.75 1.00
65_R 98_D 2.749 1.00
10_I 22_V 2.746 1.00
356_T 359_M 2.746 1.00
281_A 375_I 2.722 1.00
324_E 328_A 2.703 1.00
338_E 348_P 2.625 1.00
245_N 279_G 2.608 1.00
325_K 360_T 2.591 1.00
254_K 291_A 2.558 1.00
67_R 70_E 2.533 1.00
285_T 373_I 2.515 1.00
171_L 174_Q 2.445 1.00
177_K 183_Q 2.399 1.00
310_M 319_L 2.374 1.00
399_T 405_A 2.365 1.00
323_V 347_Y 2.356 1.00
39_A 206_A 2.245 1.00
407_Q 411_R 2.197 1.00
289_V 293_A 2.139 1.00
27_G 30_D 2.125 1.00
10_I 19_Q 2.122 1.00
379_A 407_Q 2.047 1.00
379_A 404_Q 2.016 1.00
320_H 324_E 2.015 1.00
63_A 178_D 1.968 1.00
68_S 91_A 1.945 1.00
60_I 176_F 1.941 1.00
332_R 354_N 1.935 1.00
352_L 361_A 1.931 1.00
283_A 287_R 1.929 1.00
36_Q 204_R 1.924 1.00
275_I 382_A 1.92 1.00
286_E 290_A 1.907 1.00
327_L 333_L 1.891 1.00
56_K 181_I 1.885 1.00
325_K 329_Q 1.882 1.00
164_N 312_R 1.874 1.00
241_F 256_A 1.851 1.00
274_F 371_A 1.846 1.00
69_E 87_R 1.845 1.00
390_E 436_K 1.838 1.00
334_L 353_A 1.811 1.00
63_A 66_A 1.808 1.00
125_R 455_Q 1.806 1.00
62_K 66_A 1.801 1.00
320_H 339_K 1.791 1.00
246_D 404_Q 1.779 1.00
97_C 148_V 1.778 1.00
360_T 364_E 1.773 1.00
197_S 201_K 1.771 1.00
332_R 353_A 1.767 1.00
124_Y 452_C 1.764 1.00
201_K 223_Q 1.753 1.00
320_H 347_Y 1.748 1.00
377_K 381_H 1.747 1.00
32_E 36_Q 1.738 1.00
32_E 198_Q 1.736 1.00
70_E 171_L 1.72 1.00
282_S 375_I 1.696 1.00
63_A 175_V 1.687 1.00
292_A 351_V 1.687 1.00
381_H 384_E 1.685 1.00
218_A 436_K 1.679 1.00
42_F 46_R 1.668 1.00
275_I 385_L 1.662 1.00
413_E 435_K 1.653 1.00
245_N 404_Q 1.649 1.00
62_K 98_D 1.638 1.00
289_V 334_L 1.626 1.00
281_A 285_T 1.613 1.00
380_E 411_R 1.612 1.00
333_L 352_L 1.611 1.00
69_E 73_Q 1.61 1.00
395_A 414_C 1.609 1.00
173_A 188_W 1.604 1.00
357_P 385_L 1.59 1.00
156_G 185_V 1.578 1.00
58_R 98_D 1.561 1.00
219_A 222_A 1.559 1.00
63_A 179_A 1.557 1.00
277_E 376_A 1.542 1.00
362_F 374_T 1.542 1.00
291_A 294_A 1.536 1.00
255_A 398_F 1.534 1.00
49_N 52_Y 1.533 1.00
283_A 286_E 1.53 1.00
331_A 352_L 1.516 1.00
59_D 62_K 1.511 1.00
277_E 378_D 1.503 1.00
296_K 304_E 1.499 1.00
256_A 284_F 1.485 1.00
67_R 178_D 1.483 1.00
65_R 94_A 1.478 1.00
249_L 287_R 1.468 1.00
32_E 202_D 1.457 1.00
276_I 284_F 1.455 1.00
317_D 321_H 1.452 1.00
383_L 408_M 1.45 1.00
52_Y 55_E 1.448 1.00
244_L 404_Q 1.442 1.00
321_H 360_T 1.441 1.00
287_R 290_A 1.438 1.00
74_M 171_L 1.432 1.00
47_E 411_R 1.42 1.00
324_E 327_L 1.415 1.00
378_D 381_H 1.41 1.00
124_Y 449_H 1.404 0.99
290_A 294_A 1.401 0.99
19_Q 22_V 1.391 0.99
73_Q 77_R 1.384 0.99
42_F 156_G 1.381 0.99
313_F 345_N 1.371 0.99
12_I 19_Q 1.365 0.99
256_A 274_F 1.352 0.99
316_R 320_H 1.351 0.99
73_Q 301_R 1.35 0.99
261_Y 292_A 1.348 0.99
253_V 287_R 1.337 0.99
103_H 106_A 1.332 0.99
281_A 353_A 1.329 0.99
123_E 410_A 1.328 0.99
329_Q 360_T 1.327 0.99
174_Q 178_D 1.32 0.99
290_A 293_A 1.303 0.99
132_A 157_Y 1.303 0.99
13_N 16_T 1.3 0.99
326_T 352_L 1.296 0.99
61_G 98_D 1.293 0.99
322_Q 366_M 1.29 0.99
381_H 385_L 1.285 0.99
35_L 202_D 1.281 0.99
71_M 171_L 1.281 0.99
244_L 376_A 1.277 0.99
36_Q 40_A 1.276 0.99
295_L 307_L 1.266 0.99
293_A 335_L 1.261 0.99
316_R 347_Y 1.257 0.99
97_C 144_M 1.253 0.99
197_S 223_Q 1.251 0.99
221_G 439_F 1.248 0.99
176_F 186_Y 1.246 0.98
30_D 33_N 1.233 0.98
18_E 343_A 1.23 0.98
48_T 52_Y 1.228 0.98
241_F 271_A 1.225 0.98
207_A 232_V 1.223 0.98
60_I 97_C 1.215 0.98
275_I 376_A 1.212 0.98
380_E 407_Q 1.211 0.98
376_A 385_L 1.205 0.98
83_I 87_R 1.205 0.98
313_F 316_R 1.204 0.98
197_S 219_A 1.203 0.98
76_T 86_A 1.203 0.98
244_L 408_M 1.202 0.98
59_D 63_A 1.198 0.98
143_V 159_L 1.194 0.98
60_I 175_V 1.193 0.98
409_A 457_V 1.189 0.98
383_L 411_R 1.179 0.98
242_I 382_A 1.173 0.98
61_G 65_R 1.172 0.98
21_S 167_G 1.17 0.97
35_L 204_R 1.166 0.97
363_R 388_D 1.166 0.97
132_A 209_T 1.165 0.97
383_L 387_N 1.164 0.97
123_E 457_V 1.16 0.97
68_S 87_R 1.154 0.97
219_A 223_Q 1.15 0.97
319_L 368_G 1.149 0.97
173_A 186_Y 1.148 0.97
13_N 299_D 1.147 0.97
363_R 390_E 1.146 0.97
67_R 171_L 1.144 0.97
299_D 302_D 1.14 0.97
58_R 105_P 1.137 0.97
31_I 196_V 1.126 0.97
71_M 175_V 1.124 0.97
446_F 449_H 1.121 0.97
395_A 417_V 1.12 0.96
285_T 289_V 1.118 0.96
76_T 300_P 1.116 0.96
358_E 363_R 1.114 0.96
37_L 184_G 1.11 0.96
147_A 186_Y 1.109 0.96
8_H 22_V 1.109 0.96
67_R 175_V 1.099 0.96
379_A 408_M 1.096 0.96
284_F 288_F 1.093 0.96
280_I 283_A 1.093 0.96
124_Y 454_I 1.093 0.96
216_A 219_A 1.088 0.96
288_F 371_A 1.084 0.96
282_S 286_E 1.081 0.95
316_R 339_K 1.079 0.95
380_E 384_E 1.076 0.95
48_T 56_K 1.071 0.95
277_E 280_I 1.066 0.95
357_P 388_D 1.064 0.95
327_L 352_L 1.063 0.95
244_L 277_E 1.059 0.95
401_D 404_Q 1.057 0.95
37_L 187_G 1.056 0.95
121_V 406_R 1.055 0.95
60_I 181_I 1.055 0.95
184_G 187_G 1.054 0.95
445_H 449_H 1.052 0.95
321_H 325_K 1.052 0.95
17_G 345_N 1.048 0.94
382_A 385_L 1.046 0.94
242_I 408_M 1.045 0.94
43_R 384_E 1.044 0.94
251_L 400_T 1.041 0.94
296_K 306_A 1.038 0.94
221_G 231_C 1.037 0.94
131_L 205_I 1.035 0.94
129_T 205_I 1.033 0.94
138_F 267_V 1.033 0.94
285_T 334_L 1.032 0.94
331_A 359_M 1.03 0.94
293_A 348_P 1.029 0.94
213_S 216_A 1.029 0.94
298_G 305_N 1.026 0.94
127_L 155_N 1.025 0.93
289_V 335_L 1.024 0.93
405_A 419_I 1.022 0.93
282_S 285_T 1.018 0.93
218_A 228_L 1.011 0.93
240_P 395_A 1.008 0.93
446_F 454_I 1.002 0.92
40_A 43_R 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4i8pA21100-0.002Contact Map0.791
1a4sA40.99571000.004Contact Map0.805
3k2wA80.99781000.004Contact Map0.881
3ek1A80.99351000.005Contact Map0.813
1bxsA40.99781000.006Contact Map0.836
4i9bA211000.006Contact Map0.77
1o04A80.99781000.007Contact Map0.842
1wndA40.99131000.007Contact Map0.757
4f3xA40.99571000.009Contact Map0.733
3ed6A20.99781000.009Contact Map0.78
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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