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OPENSEQ.org
THIK - Thiamine kinase
UniProt: P75948 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13432
Length: 274 (258)
Sequences: 1063
Seq/Len: 4.12
THIK
Paralog alert: 0.12 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
168_M 193_G 2.817 1.00
78_K 90_D 2.564 1.00
38_L 45_R 2.504 1.00
189_Y 246_W 2.455 1.00
80_H 90_D 2.338 1.00
210_H 236_F 2.262 1.00
107_A 213_L 2.228 1.00
61_F 64_Q 2.149 1.00
164_S 195_I 2.092 1.00
31_G 36_S 1.971 1.00
49_R 64_Q 1.924 1.00
109_L 112_Y 1.887 1.00
83_L 86_W 1.791 1.00
201_A 205_E 1.786 1.00
262_L 266_T 1.744 0.99
258_Q 262_L 1.726 0.99
205_E 243_K 1.712 0.99
47_V 51_P 1.696 0.99
94_G 176_V 1.687 0.99
257_Q 266_T 1.652 0.99
147_R 150_K 1.595 0.99
166_L 193_G 1.586 0.99
128_L 242_L 1.571 0.98
211_R 229_W 1.565 0.98
214_V 229_W 1.563 0.98
34_G 61_F 1.537 0.98
204_V 240_L 1.522 0.98
130_E 153_R 1.514 0.98
203_W 210_H 1.511 0.98
109_L 184_L 1.508 0.98
52_H 56_A 1.447 0.97
32_L 171_H 1.438 0.97
195_I 231_Q 1.438 0.97
44_Q 90_D 1.415 0.96
126_L 153_R 1.414 0.96
52_H 55_D 1.41 0.96
203_W 213_L 1.4 0.96
132_Y 242_L 1.393 0.96
200_A 232_V 1.363 0.95
211_R 226_A 1.358 0.95
150_K 154_K 1.344 0.95
260_I 267_W 1.338 0.95
243_K 247_F 1.329 0.94
265_D 268_R 1.315 0.94
110_L 170_V 1.312 0.94
114_H 217_Y 1.304 0.94
13_E 16_S 1.303 0.94
108_G 216_D 1.298 0.93
133_W 245_G 1.293 0.93
113_L 184_L 1.291 0.93
109_L 182_L 1.287 0.93
71_L 74_C 1.269 0.92
240_L 243_K 1.263 0.92
103_T 202_V 1.257 0.92
31_G 171_H 1.249 0.92
13_E 17_R 1.234 0.91
33_S 64_Q 1.23 0.91
168_M 197_L 1.227 0.91
170_V 184_L 1.226 0.91
32_L 49_R 1.215 0.90
13_E 21_Q 1.215 0.90
262_L 267_W 1.214 0.90
49_R 89_V 1.197 0.89
14_L 20_P 1.192 0.89
119_F 165_P 1.189 0.89
196_A 231_Q 1.188 0.89
38_L 91_Y 1.186 0.89
16_S 21_Q 1.176 0.88
261_R 265_D 1.176 0.88
48_V 88_V 1.174 0.88
175_L 184_L 1.167 0.88
75_I 93_P 1.167 0.88
49_R 189_Y 1.164 0.87
77_P 90_D 1.164 0.87
200_A 230_R 1.153 0.87
51_P 71_L 1.147 0.86
32_L 189_Y 1.141 0.86
51_P 89_V 1.137 0.86
264_D 269_Q 1.134 0.85
17_R 21_Q 1.123 0.85
104_N 216_D 1.123 0.85
188_E 246_W 1.12 0.84
149_L 153_R 1.115 0.84
17_R 24_P 1.112 0.84
94_G 97_K 1.107 0.84
187_W 247_F 1.105 0.83
202_V 206_N 1.092 0.82
123_I 132_Y 1.091 0.82
134_Q 154_K 1.088 0.82
220_R 228_L 1.082 0.82
256_D 263_A 1.076 0.81
92_L 183_K 1.075 0.81
31_G 132_Y 1.07 0.81
55_D 61_F 1.068 0.80
67_A 165_P 1.058 0.80
61_F 189_Y 1.056 0.79
68_L 79_P 1.055 0.79
108_G 115_Q 1.054 0.79
52_H 61_F 1.053 0.79
49_R 188_E 1.052 0.79
19_F 24_P 1.048 0.79
87_M 171_H 1.046 0.78
165_P 193_G 1.044 0.78
257_Q 262_L 1.041 0.78
33_S 60_A 1.041 0.78
190_A 249_Y 1.04 0.78
184_L 235_W 1.037 0.78
129_L 241_M 1.034 0.77
72_P 79_P 1.029 0.77
61_F 188_E 1.028 0.77
92_L 104_N 1.025 0.76
256_D 259_F 1.025 0.76
205_E 240_L 1.023 0.76
26_T 40_E 1.021 0.76
258_Q 266_T 1.018 0.76
131_L 249_Y 1.006 0.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1nw1A20.99641000.337Contact Map0.549
2qg7A411000.354Contact Map0.596
3c5iA411000.382Contact Map0.544
3dxqA20.95621000.383Contact Map0.502
4da5A211000.39Contact Map0.55
3mesA211000.393Contact Map0.515
3f2sA211000.402Contact Map0.552
3fegA111000.408Contact Map0.574
1zylA10.981899.90.595Contact Map0.459
3r70A10.959999.90.604Contact Map0.421
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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