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OPENSEQ.org
RUTA - Pyrimidine monooxygenase RutA
UniProt: P75898 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13859
Length: 382 (355)
Sequences: 5330
Seq/Len: 15.01
RUTA
Paralog alert: 0.66 [within 20: 0.18] - ratio of genomes with paralogs
Cluster includes: RUTA SSUD YHBW
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
268_D 329_S 7.091 1.00
268_D 326_S 3.87 1.00
54_Q 96_V 3.418 1.00
182_K 187_T 3.365 1.00
161_D 184_D 3.07 1.00
360_F 364_I 3.044 1.00
55_K 58_H 2.95 1.00
335_D 339_S 2.905 1.00
164_T 218_R 2.886 1.00
328_A 367_L 2.849 1.00
55_K 358_E 2.833 1.00
267_T 270_A 2.766 1.00
57_E 99_R 2.741 1.00
90_M 102_I 2.725 1.00
333_M 336_E 2.707 1.00
268_D 272_R 2.67 1.00
59_Y 361_G 2.648 1.00
237_A 241_A 2.556 1.00
214_A 246_A 2.517 1.00
217_A 246_A 2.488 1.00
358_E 362_E 2.409 1.00
266_E 363_R 2.343 1.00
269_D 273_A 2.338 1.00
367_L 370_C 2.304 1.00
241_A 245_Q 2.3 1.00
331_A 367_L 2.291 1.00
50_K 54_Q 2.257 1.00
54_Q 58_H 2.223 1.00
326_S 329_S 2.22 1.00
240_A 256_S 2.207 1.00
265_D 270_A 2.165 1.00
53_V 64_A 2.077 1.00
247_A 254_V 2.068 1.00
328_A 332_R 2.061 1.00
47_E 128_G 2.031 1.00
355_S 358_E 2.014 1.00
276_E 280_A 1.991 1.00
101_Q 132_G 1.944 1.00
175_G 200_P 1.942 1.00
51_A 54_Q 1.927 1.00
161_D 185_F 1.919 1.00
94_A 127_S 1.906 1.00
93_L 102_I 1.897 1.00
154_D 158_R 1.897 1.00
158_R 184_D 1.891 1.00
168_Q 219_Y 1.869 1.00
165_E 183_G 1.865 1.00
50_K 96_V 1.832 1.00
120_A 131_F 1.825 1.00
60_H 369_Q 1.824 1.00
124_D 129_G 1.762 1.00
53_V 93_L 1.751 1.00
172_D 176_T 1.742 1.00
336_E 340_V 1.703 1.00
270_A 274_K 1.7 1.00
266_E 270_A 1.696 1.00
258_V 343_A 1.688 1.00
58_H 98_S 1.662 1.00
180_D 190_D 1.659 1.00
213_M 239_T 1.652 1.00
264_A 330_V 1.635 1.00
94_A 102_I 1.634 1.00
327_Y 359_T 1.575 1.00
168_Q 181_F 1.557 1.00
64_A 100_I 1.548 1.00
214_A 249_Q 1.548 1.00
214_A 218_R 1.535 1.00
359_T 363_R 1.531 1.00
264_A 359_T 1.526 1.00
245_Q 249_Q 1.524 1.00
56_A 357_I 1.515 1.00
135_L 167_V 1.514 1.00
180_D 189_N 1.486 1.00
57_E 98_S 1.485 1.00
55_K 59_Y 1.482 1.00
270_A 273_A 1.482 1.00
329_S 332_R 1.478 1.00
246_A 249_Q 1.476 1.00
66_S 90_M 1.447 1.00
168_Q 172_D 1.444 1.00
365_Q 369_Q 1.434 1.00
323_L 330_V 1.434 1.00
65_L 103_Y 1.416 1.00
158_R 161_D 1.411 1.00
103_Y 132_G 1.409 1.00
171_R 200_P 1.403 0.99
214_A 250_T 1.4 0.99
85_E 88_T 1.399 0.99
59_Y 357_I 1.388 0.99
51_A 354_L 1.385 0.99
164_T 219_Y 1.38 0.99
158_R 185_F 1.379 0.99
85_E 119_M 1.378 0.99
217_A 250_T 1.378 0.99
267_T 271_A 1.371 0.99
238_P 241_A 1.367 0.99
50_K 92_G 1.366 0.99
39_A 42_Y 1.361 0.99
47_E 51_A 1.359 0.99
264_A 327_Y 1.352 0.99
61_F 361_G 1.338 0.99
111_L 119_M 1.321 0.99
335_D 338_A 1.313 0.99
55_K 354_L 1.307 0.99
235_A 238_P 1.295 0.99
51_A 55_K 1.294 0.99
169_V 188_M 1.294 0.99
120_A 133_V 1.29 0.99
172_D 179_S 1.286 0.99
237_A 340_V 1.282 0.99
23_G 63_F 1.276 0.99
274_K 359_T 1.266 0.99
171_R 203_V 1.259 0.99
255_G 344_E 1.256 0.99
336_E 339_S 1.256 0.99
47_E 50_K 1.253 0.99
363_R 367_L 1.247 0.99
169_V 181_F 1.246 0.98
92_G 126_I 1.242 0.98
275_W 324_V 1.241 0.98
66_S 93_L 1.237 0.98
213_M 246_A 1.236 0.98
46_F 126_I 1.22 0.98
21_K 62_D 1.216 0.98
273_A 276_E 1.213 0.98
171_R 219_Y 1.202 0.98
172_D 181_F 1.202 0.98
205_C 212_G 1.198 0.98
169_V 186_F 1.19 0.98
332_R 335_D 1.184 0.98
242_R 246_A 1.183 0.98
312_P 316_V 1.178 0.98
210_D 246_A 1.177 0.98
275_W 279_K 1.177 0.98
260_F 334_L 1.176 0.98
265_D 271_A 1.173 0.98
331_A 335_D 1.171 0.98
207_G 223_N 1.164 0.97
92_G 95_A 1.164 0.97
266_E 355_S 1.159 0.97
148_G 151_P 1.157 0.97
169_V 172_D 1.148 0.97
218_R 250_T 1.147 0.97
332_R 336_E 1.146 0.97
332_R 371_R 1.142 0.97
367_L 371_R 1.137 0.97
171_R 221_D 1.133 0.97
157_S 211_A 1.128 0.97
354_L 358_E 1.128 0.97
84_L 122_T 1.126 0.97
182_K 189_N 1.125 0.97
94_A 131_F 1.125 0.97
350_F 360_F 1.123 0.97
62_D 369_Q 1.121 0.97
188_M 191_C 1.12 0.96
262_V 360_F 1.115 0.96
331_A 364_I 1.113 0.96
47_E 98_S 1.108 0.96
141_K 145_E 1.105 0.96
164_T 168_Q 1.097 0.96
145_E 189_N 1.097 0.96
123_I 131_F 1.091 0.96
355_S 359_T 1.084 0.96
124_D 198_S 1.075 0.95
181_F 186_F 1.069 0.95
325_G 330_V 1.069 0.95
53_V 100_I 1.069 0.95
115_I 119_M 1.065 0.95
88_T 91_A 1.063 0.95
53_V 97_T 1.055 0.95
237_A 341_P 1.051 0.94
161_D 211_A 1.047 0.94
263_I 274_K 1.041 0.94
92_G 96_V 1.034 0.94
215_F 219_Y 1.031 0.94
58_H 358_E 1.028 0.94
238_P 242_R 1.026 0.94
204_I 257_Y 1.024 0.93
167_V 219_Y 1.012 0.93
348_L 360_F 1.009 0.93
46_F 92_G 1.009 0.93
57_E 97_T 1.006 0.93
20_M 338_A 1.001 0.92
273_A 277_H 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3b9oA20.95031000.293Contact Map0.767
1tvlA10.95811000.294Contact Map0.66
1nqkA10.90841000.295Contact Map0.7
3sdoA20.96071000.297Contact Map0.666
2wgkA20.87961000.312Contact Map0.66
1rhcA10.83251000.318Contact Map0.605
1lucA10.86651000.318Contact Map0.628
3raoA20.9451000.322Contact Map0.725
1ezwA10.84031000.325Contact Map0.688
3c8nA40.8771000.328Contact Map0.643
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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