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OPENSEQ.org

RUTE - Probable malonic semialdehyde reductase RutE
UniProt: P75894 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13855
Length: 196 (190)
Sequences: 5499
Seq/Len: 28.94

RUTE
Paralog alert: 0.79 [within 20: 0.04] - ratio of genomes with paralogs
Cluster includes: NFNB RUTE YDJA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
29_S 32_T 4.205 1.00
35_E 139_L 3.724 1.00
30_D 58_R 3.056 1.00
54_I 128_Q 2.939 1.00
36_I 136_C 2.84 1.00
30_D 34_R 2.69 1.00
26_T 174_Y 2.592 1.00
38_A 41_K 2.384 1.00
142_D 175_G 2.379 1.00
133_I 143_T 2.324 1.00
141_L 174_Y 2.313 1.00
53_R 194_G 2.262 1.00
54_I 85_A 2.256 1.00
16_D 137_R 2.214 1.00
32_T 141_L 2.039 1.00
65_R 157_A 1.959 1.00
32_T 139_L 1.935 1.00
40_M 132_L 1.933 1.00
60_A 64_E 1.897 1.00
55_V 88_A 1.831 1.00
155_D 166_S 1.824 1.00
136_C 172_I 1.824 1.00
141_L 172_I 1.824 1.00
153_H 156_D 1.793 1.00
12_T 16_D 1.786 1.00
67_R 79_L 1.777 1.00
64_E 79_L 1.756 1.00
85_A 132_L 1.723 1.00
62_G 65_R 1.717 1.00
10_L 14_F 1.71 1.00
36_I 132_L 1.667 1.00
129_A 168_L 1.661 1.00
187_L 191_E 1.622 1.00
39_L 135_A 1.612 1.00
129_A 170_I 1.591 1.00
88_A 164_L 1.59 1.00
123_R 126_S 1.587 1.00
37_Y 193_C 1.562 1.00
36_I 172_I 1.527 1.00
32_T 174_Y 1.521 1.00
22_G 176_D 1.507 1.00
64_E 68_P 1.449 1.00
24_R 176_D 1.432 1.00
23_W 171_N 1.422 1.00
34_R 190_E 1.422 1.00
86_I 169_L 1.417 1.00
10_L 13_L 1.416 1.00
56_F 83_V 1.415 1.00
133_I 170_I 1.414 1.00
66_L 154_V 1.387 0.99
127_M 130_A 1.38 0.99
69_A 157_A 1.366 0.99
136_C 143_T 1.354 0.99
125_S 167_N 1.35 0.99
32_T 35_E 1.348 0.99
145_P 168_L 1.334 0.99
36_I 141_L 1.333 0.99
28_V 141_L 1.326 0.99
189_F 193_C 1.312 0.99
60_A 63_K 1.294 0.99
39_L 139_L 1.285 0.99
27_P 58_R 1.285 0.99
59_T 62_G 1.285 0.99
87_V 129_A 1.28 0.99
61_E 64_E 1.277 0.99
65_R 68_P 1.277 0.99
69_A 153_H 1.275 0.99
133_I 145_P 1.267 0.99
61_E 65_R 1.267 0.99
137_R 142_D 1.261 0.99
62_G 157_A 1.26 0.99
34_R 38_A 1.255 0.99
15_T 38_A 1.238 0.98
150_D 153_H 1.232 0.98
29_S 174_Y 1.213 0.98
89_W 121_A 1.197 0.98
90_D 163_T 1.191 0.98
33_L 83_V 1.164 0.97
152_Q 156_D 1.163 0.97
43_G 131_Y 1.156 0.97
19_T 144_G 1.153 0.97
12_T 15_T 1.152 0.97
14_F 134_V 1.152 0.97
88_A 160_T 1.15 0.97
53_R 164_L 1.131 0.97
56_F 193_C 1.121 0.97
31_E 34_R 1.119 0.96
28_V 32_T 1.111 0.96
126_S 145_P 1.097 0.96
143_T 172_I 1.094 0.96
58_R 83_V 1.058 0.95
86_I 166_S 1.038 0.94
28_V 174_Y 1.038 0.94
132_L 170_I 1.036 0.94
107_R 111_T 1.027 0.94
130_A 134_V 1.025 0.93
34_R 189_F 1.017 0.93
15_T 42_W 1.017 0.93
86_I 158_F 1.013 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ge6A20.97961000.138Contact Map0.786
3gbhA40.98471000.144Contact Map0.847
1noxA10.97961000.147Contact Map0.774
3bemA20.98981000.155Contact Map0.869
1icrA20.96431000.155Contact Map0.794
3of4A30.95921000.155Contact Map0.816
3eofA20.91331000.159Contact Map0.81
1zchA10.92861000.161Contact Map0.773
3gagA40.9491000.162Contact Map0.836
2h0uA10.95921000.163Contact Map0.752

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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